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The map label for this gene is atoC [H]

Identifier: 82703784

GI number: 82703784

Start: 3067053

End: 3068402

Strand: Reverse

Name: atoC [H]

Synonym: Nmul_A2671

Alternate gene names: 82703784

Gene position: 3068402-3067053 (Counterclockwise)

Preceding gene: 82703785

Following gene: 82703783

Centisome position: 96.36

GC content: 53.85

Gene sequence:

>1350_bases
ATGAGTCAGGACAAGAAGAGTCTGTTGGTAATCGAGGATGATCCCGGATTGCAACGCCAATTGCGCTGGAGCTTCGATAC
TTATGAAGTGTTGGTGGCAAGCGACCGCGAAAGCGCGCTTGCTCTGGTCAGACGGCATGAGCCCGCCGTCGTCACCATGG
ATCTGGGTCTTCCTCCCGATGCGGATGGCGCTTCCGAAGGTTTGGCTACGTTGGAACAGATACTTGAACTGGTGCCGGAT
ACGAAGGTAATTGTTCTCACCGGGAATCAGGACCATGGAAATGCTCTCAAGGCTATCGCTGCAGGGGCTTATGACTTTCA
TCAGAAGCCCTTTGATCCCAATATCCTGAGCCTGGTGATAGAACGGGCATACTATCTCTATGCTCTGCAGCAGGAGAATC
GCAAGCTGTTGCAGGCCCAGATGAACTCTCCCATCAACGGCCTCATAACCCGCGATCCCGTCTTGAGCAAGGTATGCCGC
AACGTGGAAAAAGTCGCACCCTCGGACGCTACGGTTATACTGCTGGGCGAGAGTGGTACCGGCAAGGAAATTCTGGCACG
GGCGCTGCATCAGTTGAGCGCCCGGCGGGAGAAGCGCTTCATGGCCATTAATTGTGCAGCCATCCCGGAGTCGTTGCTGG
AGAGTGAATTGTTCGGCTATGAAAAGGGTGCTTATACGGGAGCCGTCAAACAGACGCCCGGAAAAATCGAGCTCGCGCAC
GAAGGCACTTTTTTTCTTGACGAAGTGGGCGATCTGCCCATGCCCTTGCAGGCAAAGCTTCTCCGTTTTTTGCAGGAAAG
GGTGATCGAGCGAATAGGGGGTCGGAAGGAAATACCCGTGGATGTGCGCGTGGTATGCGCCACTCATCAGAATCTCAAGA
AACTGATCGAGGATGGACGTTTTCGCGAAGATTTGTATTACCGCTTGAGTGAAATCGTGATAACCATTCCTCCCTTGCGA
CAGCGGGTGGGGGATGCCGCGCTGCTTGCCCATCATTTCAAGAACAAGTTTTGCATGCAGGAGAAACGTTCCTCTCTGAA
TTTCAGCCAGGAAGCGCTGGCTGCCATTGAAAATCATTCGTGGCCGGGCAACGTGCGCGAAATGGAAAACTGTATCAAGC
GTGCGGTCATCATGGCAGAGGGTTCGGTAATCGGCGCGGATGACCTCGGCCTGTACGGTACAAGCGCAGCTGAGCCTATC
AACCTTCGCCAGATACGGGACAGGGCGGAATGCAATGCGCTGATGAAAGCATTGGCGAGAGTGGATGGGAACGTGGTGAA
GGCGGCTGAATTACTGGGCGTCAGCCGTCCCACTATTTATGATCTGATGAATCGCCATGGTTTGAAGTAA

Upstream 100 bases:

>100_bases
GATCATTCTGCCTCTTTACGGAGCGCGGACTGTGGGTATTCAGAAGGCGGGCTGATTTAATGAATTCGAGGGACCGTAAT
ACCGGTAATAAGAGGACTGA

Downstream 100 bases:

>100_bases
TACGGTGCAGATGCGCATTCCATATCAAAATTACAGACAACAGGCTTGCTTCCAATGACGCACCTTATCCAGGATCTCGT
TTTCGAATCTGCCAATCGTT

Product: two component sigma-54 specific Fis family transcriptional regulator

Products: NA

Alternate protein names: Ornithine decarboxylase antizyme; Ornithine/arginine decarboxylase inhibitor [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MSQDKKSLLVIEDDPGLQRQLRWSFDTYEVLVASDRESALALVRRHEPAVVTMDLGLPPDADGASEGLATLEQILELVPD
TKVIVLTGNQDHGNALKAIAAGAYDFHQKPFDPNILSLVIERAYYLYALQQENRKLLQAQMNSPINGLITRDPVLSKVCR
NVEKVAPSDATVILLGESGTGKEILARALHQLSARREKRFMAINCAAIPESLLESELFGYEKGAYTGAVKQTPGKIELAH
EGTFFLDEVGDLPMPLQAKLLRFLQERVIERIGGRKEIPVDVRVVCATHQNLKKLIEDGRFREDLYYRLSEIVITIPPLR
QRVGDAALLAHHFKNKFCMQEKRSSLNFSQEALAAIENHSWPGNVREMENCIKRAVIMAEGSVIGADDLGLYGTSAAEPI
NLRQIRDRAECNALMKALARVDGNVVKAAELLGVSRPTIYDLMNRHGLK

Sequences:

>Translated_449_residues
MSQDKKSLLVIEDDPGLQRQLRWSFDTYEVLVASDRESALALVRRHEPAVVTMDLGLPPDADGASEGLATLEQILELVPD
TKVIVLTGNQDHGNALKAIAAGAYDFHQKPFDPNILSLVIERAYYLYALQQENRKLLQAQMNSPINGLITRDPVLSKVCR
NVEKVAPSDATVILLGESGTGKEILARALHQLSARREKRFMAINCAAIPESLLESELFGYEKGAYTGAVKQTPGKIELAH
EGTFFLDEVGDLPMPLQAKLLRFLQERVIERIGGRKEIPVDVRVVCATHQNLKKLIEDGRFREDLYYRLSEIVITIPPLR
QRVGDAALLAHHFKNKFCMQEKRSSLNFSQEALAAIENHSWPGNVREMENCIKRAVIMAEGSVIGADDLGLYGTSAAEPI
NLRQIRDRAECNALMKALARVDGNVVKAAELLGVSRPTIYDLMNRHGLK
>Mature_448_residues
SQDKKSLLVIEDDPGLQRQLRWSFDTYEVLVASDRESALALVRRHEPAVVTMDLGLPPDADGASEGLATLEQILELVPDT
KVIVLTGNQDHGNALKAIAAGAYDFHQKPFDPNILSLVIERAYYLYALQQENRKLLQAQMNSPINGLITRDPVLSKVCRN
VEKVAPSDATVILLGESGTGKEILARALHQLSARREKRFMAINCAAIPESLLESELFGYEKGAYTGAVKQTPGKIELAHE
GTFFLDEVGDLPMPLQAKLLRFLQERVIERIGGRKEIPVDVRVVCATHQNLKKLIEDGRFREDLYYRLSEIVITIPPLRQ
RVGDAALLAHHFKNKFCMQEKRSSLNFSQEALAAIENHSWPGNVREMENCIKRAVIMAEGSVIGADDLGLYGTSAAEPIN
LRQIRDRAECNALMKALARVDGNVVKAAELLGVSRPTIYDLMNRHGLK

Specific function: Member of the two-component regulatory system AtoS/AtoC involved in the transcriptional regulation of the ato genes for acetoacetate metabolism. Also an inhibitor of polyamine biosynthesis [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=455, Percent_Identity=39.5604395604396, Blast_Score=333, Evalue=2e-92,
Organism=Escherichia coli, GI1790437, Length=456, Percent_Identity=38.3771929824561, Blast_Score=301, Evalue=7e-83,
Organism=Escherichia coli, GI1788905, Length=453, Percent_Identity=37.7483443708609, Blast_Score=281, Evalue=8e-77,
Organism=Escherichia coli, GI1790299, Length=430, Percent_Identity=37.4418604651163, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI87082117, Length=340, Percent_Identity=43.5294117647059, Blast_Score=266, Evalue=2e-72,
Organism=Escherichia coli, GI1789087, Length=313, Percent_Identity=43.4504792332268, Blast_Score=254, Evalue=9e-69,
Organism=Escherichia coli, GI1789233, Length=269, Percent_Identity=46.0966542750929, Blast_Score=234, Evalue=7e-63,
Organism=Escherichia coli, GI87082152, Length=318, Percent_Identity=39.622641509434, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI87081872, Length=307, Percent_Identity=38.4364820846906, Blast_Score=215, Evalue=4e-57,
Organism=Escherichia coli, GI1786524, Length=312, Percent_Identity=39.4230769230769, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI1787583, Length=318, Percent_Identity=35.2201257861635, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI87081858, Length=306, Percent_Identity=32.3529411764706, Blast_Score=150, Evalue=1e-37,
Organism=Escherichia coli, GI1789828, Length=294, Percent_Identity=30.952380952381, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49906; Mature: 49775

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQDKKSLLVIEDDPGLQRQLRWSFDTYEVLVASDRESALALVRRHEPAVVTMDLGLPPD
CCCCCCCEEEEECCCCCHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCEEEEEECCCCCC
ADGASEGLATLEQILELVPDTKVIVLTGNQDHGNALKAIAAGAYDFHQKPFDPNILSLVI
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCHHHCCCCCCCHHHHHHH
ERAYYLYALQQENRKLLQAQMNSPINGLITRDPVLSKVCRNVEKVAPSDATVILLGESGT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHCCCCCEEEEEECCCC
GKEILARALHQLSARREKRFMAINCAAIPESLLESELFGYEKGAYTGAVKQTPGKIELAH
CHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEE
EGTFFLDEVGDLPMPLQAKLLRFLQERVIERIGGRKEIPVDVRVVCATHQNLKKLIEDGR
CCCEEHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHCCC
FREDLYYRLSEIVITIPPLRQRVGDAALLAHHFKNKFCMQEKRSSLNFSQEALAAIENHS
HHHHHHHHHHHHEEECCHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
WPGNVREMENCIKRAVIMAEGSVIGADDLGLYGTSAAEPINLRQIRDRAECNALMKALAR
CCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VDGNVVKAAELLGVSRPTIYDLMNRHGLK
HCCCHHHHHHHHCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
SQDKKSLLVIEDDPGLQRQLRWSFDTYEVLVASDRESALALVRRHEPAVVTMDLGLPPD
CCCCCCEEEEECCCCCHHHHHCCCCCEEEEEECCCHHHHHHHHCCCCEEEEEECCCCCC
ADGASEGLATLEQILELVPDTKVIVLTGNQDHGNALKAIAAGAYDFHQKPFDPNILSLVI
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCHHHCCCCCCCHHHHHHH
ERAYYLYALQQENRKLLQAQMNSPINGLITRDPVLSKVCRNVEKVAPSDATVILLGESGT
HHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHCCCCCEEEEEECCCC
GKEILARALHQLSARREKRFMAINCAAIPESLLESELFGYEKGAYTGAVKQTPGKIELAH
CHHHHHHHHHHHHHHHHHHEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEE
EGTFFLDEVGDLPMPLQAKLLRFLQERVIERIGGRKEIPVDVRVVCATHQNLKKLIEDGR
CCCEEHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHCCC
FREDLYYRLSEIVITIPPLRQRVGDAALLAHHFKNKFCMQEKRSSLNFSQEALAAIENHS
HHHHHHHHHHHHEEECCHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCC
WPGNVREMENCIKRAVIMAEGSVIGADDLGLYGTSAAEPINLRQIRDRAECNALMKALAR
CCCCHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
VDGNVVKAAELLGVSRPTIYDLMNRHGLK
HCCCHHHHHHHHCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 8346225 [H]