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The map label for this gene is asnB [H]

Identifier: 82703781

GI number: 82703781

Start: 3062155

End: 3064044

Strand: Reverse

Name: asnB [H]

Synonym: Nmul_A2668

Alternate gene names: 82703781

Gene position: 3064044-3062155 (Counterclockwise)

Preceding gene: 82703782

Following gene: 82703780

Centisome position: 96.23

GC content: 54.02

Gene sequence:

>1890_bases
GTGAGTGGATTATGCGGTTGGCTGGGATATGGCCATTCAGTTACTGAAAACCTGGCGCTGCTCCGGCAAATGGCAAGCCC
GCTCGTACGGTTTGACAGTTGTAAGTTGCACGCCTGCGCGAGCGGGAATAGCGCTTTAGCAACCGCTGCTTCGGACGGGC
GTGCTCACATTTATCAGAGGGATGGGCTTCTTGCCGCGTTGTGGGGACGGGGAAATTTTCTGGAACCCCGCCTGGATGAA
CAGGCGCAACGCGAAGGCATGGCGAAAACGCTGGCCGAAAATTGGCGGGGAAGGAGTGGAGGGGACGGAAGAGGCGCAAA
GATTTTCGAGAGCCTGAGCGGTGCGTTTGCATGCTGTATTGTCGATGAAAATGCGAATGAGGTCGTTCTGGCGACAGACC
GTATGGGCACTTACCCACTCTCCTACCAGGCTCTGGCCGGAGGCTTGGTATTTGCCTCGTCATCAGATGCCATCCGCCTG
CATCCTTCGATCAGGTCGGAAATCGACCTCCAGAGTCTGTATAACTACGTTTATTTTCATATGGTGCCTAGTCCTGACAC
CATTTATGAGGGGCAGAAACGCCTTATGCCGGGCGAATATCTCGTCTACAGTAAGGGCGGCATCAAGACTGAAAAATACT
GGCTGATGCGGTTTCAGGAAGATGAAAGACACCCTTTCCAGGAACTCAAGCAGGAGTTTCTGGAAGTATTGCATTCCAGT
GTGCGGACCGCCATTGGCGATGACGAAACGGGAGCCTTTCTCAGTGGCGGAACCGACAGTTCGACGATTGCCGGGATACT
GGGCGAGGTGAGCGGACAGCCAGCACGCACCTATTCCATTGGTTTCGATGCAACGGGTTATGATGAAATGGGATATGCCC
GGATCGCAGCCAGGCATTTCGGTACGCGGCATCAGGAGTACTACGTTACACCGGAGAATATCGTCTCCGCCATTCCCCAG
GTTGCAGCGGTATTCGATCAGCCCTTCGGCAACTCTTCCGCGTTACCCGCCTACTACTGCGCGCAGATGGCAAAATCCGA
CGGCCTCAGCAGAATGCTCGGCGGAGATGGGGGGGATGAGCTTTTTGGCGGTAACGTACGTTATGCCAAACAACACATTT
TCTCGCTGTATGAGCAGACTCCCTTGTTATTGCGCAAGGGACTCATCGAACCGGTTGTTTTTGGTCTTCCTGGGGGAGGC
GCGCTTCCCCTGATACGGAAAGCGCGCAGTTATGTCGAGCAGGCATCCATCCCCATGCCTGCACGCACCGAGACTTACAA
TCTTCTTGAACGTTACGGACGCGACGCTATCTTTACCCCTGAATTTCTCGATGCGGTAAATCCGGGTTATCCGGCGCGCC
TGCTGGACGAGACTTATCATGAGAATGATGCCAAAAGCCTGATCAATCAAATGCTTGCATTTGATCGCAAATTCACCCTC
GCCGATAACGACTTGCCCAAGGTCGCCAAGGCGTGCGAGTTGGCGGGAATGGACGTTGCGTTTCCACTCATCAGCGATGA
GATCGTTGCTTTTTCCCTGCGGCTCGAACCTCACCTGAAGCTCAAGGGGACGAAGTTGCGCTACTTCTTCAAGGAAGCCC
TGCGGGGATTTTTGCCGGACGATATTATTGCCAAGCAAAAACATGGTTTCGGATTACCTTTCGGGGTGTGGCTTCAAACC
CATAAAAACCTGCAGGACCTGGCGTGGGATAGTCTGAGCGATCTCAAATCGAGAAACATCATCCGCGGTGACTTTATCGA
GAAGCTGCTGCAGCAGCACCTGGAGGAGCATGCCGGATATCACGGCACCATGGTATGGGTGCTGATGATGCTGGAGCAAT
GGTACAGGCAGCGACAGGACTCTCGAAGTGCAATGCATTCTCAGGCATGA

Upstream 100 bases:

>100_bases
GTTATGCCTTTTGACCAAGAGGGTGGCAAAAAGCAAATAATCCACAAAAAATCCATCAGATAACGAGAAGCACAATGCCA
AGAAAACGTGGAGAATTCCT

Downstream 100 bases:

>100_bases
GAACCGGGATAAGTAACAGATGATCCGTTGCGTTTCCAGGCCCAAGAAGTGCTGAATAACAGAGCAGGTCCTCCTGAATA
CTCCTGCCCTGACGGTTTGC

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 629; Mature: 628

Protein sequence:

>629_residues
MSGLCGWLGYGHSVTENLALLRQMASPLVRFDSCKLHACASGNSALATAASDGRAHIYQRDGLLAALWGRGNFLEPRLDE
QAQREGMAKTLAENWRGRSGGDGRGAKIFESLSGAFACCIVDENANEVVLATDRMGTYPLSYQALAGGLVFASSSDAIRL
HPSIRSEIDLQSLYNYVYFHMVPSPDTIYEGQKRLMPGEYLVYSKGGIKTEKYWLMRFQEDERHPFQELKQEFLEVLHSS
VRTAIGDDETGAFLSGGTDSSTIAGILGEVSGQPARTYSIGFDATGYDEMGYARIAARHFGTRHQEYYVTPENIVSAIPQ
VAAVFDQPFGNSSALPAYYCAQMAKSDGLSRMLGGDGGDELFGGNVRYAKQHIFSLYEQTPLLLRKGLIEPVVFGLPGGG
ALPLIRKARSYVEQASIPMPARTETYNLLERYGRDAIFTPEFLDAVNPGYPARLLDETYHENDAKSLINQMLAFDRKFTL
ADNDLPKVAKACELAGMDVAFPLISDEIVAFSLRLEPHLKLKGTKLRYFFKEALRGFLPDDIIAKQKHGFGLPFGVWLQT
HKNLQDLAWDSLSDLKSRNIIRGDFIEKLLQQHLEEHAGYHGTMVWVLMMLEQWYRQRQDSRSAMHSQA

Sequences:

>Translated_629_residues
MSGLCGWLGYGHSVTENLALLRQMASPLVRFDSCKLHACASGNSALATAASDGRAHIYQRDGLLAALWGRGNFLEPRLDE
QAQREGMAKTLAENWRGRSGGDGRGAKIFESLSGAFACCIVDENANEVVLATDRMGTYPLSYQALAGGLVFASSSDAIRL
HPSIRSEIDLQSLYNYVYFHMVPSPDTIYEGQKRLMPGEYLVYSKGGIKTEKYWLMRFQEDERHPFQELKQEFLEVLHSS
VRTAIGDDETGAFLSGGTDSSTIAGILGEVSGQPARTYSIGFDATGYDEMGYARIAARHFGTRHQEYYVTPENIVSAIPQ
VAAVFDQPFGNSSALPAYYCAQMAKSDGLSRMLGGDGGDELFGGNVRYAKQHIFSLYEQTPLLLRKGLIEPVVFGLPGGG
ALPLIRKARSYVEQASIPMPARTETYNLLERYGRDAIFTPEFLDAVNPGYPARLLDETYHENDAKSLINQMLAFDRKFTL
ADNDLPKVAKACELAGMDVAFPLISDEIVAFSLRLEPHLKLKGTKLRYFFKEALRGFLPDDIIAKQKHGFGLPFGVWLQT
HKNLQDLAWDSLSDLKSRNIIRGDFIEKLLQQHLEEHAGYHGTMVWVLMMLEQWYRQRQDSRSAMHSQA
>Mature_628_residues
SGLCGWLGYGHSVTENLALLRQMASPLVRFDSCKLHACASGNSALATAASDGRAHIYQRDGLLAALWGRGNFLEPRLDEQ
AQREGMAKTLAENWRGRSGGDGRGAKIFESLSGAFACCIVDENANEVVLATDRMGTYPLSYQALAGGLVFASSSDAIRLH
PSIRSEIDLQSLYNYVYFHMVPSPDTIYEGQKRLMPGEYLVYSKGGIKTEKYWLMRFQEDERHPFQELKQEFLEVLHSSV
RTAIGDDETGAFLSGGTDSSTIAGILGEVSGQPARTYSIGFDATGYDEMGYARIAARHFGTRHQEYYVTPENIVSAIPQV
AAVFDQPFGNSSALPAYYCAQMAKSDGLSRMLGGDGGDELFGGNVRYAKQHIFSLYEQTPLLLRKGLIEPVVFGLPGGGA
LPLIRKARSYVEQASIPMPARTETYNLLERYGRDAIFTPEFLDAVNPGYPARLLDETYHENDAKSLINQMLAFDRKFTLA
DNDLPKVAKACELAGMDVAFPLISDEIVAFSLRLEPHLKLKGTKLRYFFKEALRGFLPDDIIAKQKHGFGLPFGVWLQTH
KNLQDLAWDSLSDLKSRNIIRGDFIEKLLQQHLEEHAGYHGTMVWVLMMLEQWYRQRQDSRSAMHSQA

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786889, Length=295, Percent_Identity=24.7457627118644, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 70014; Mature: 69883

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLCGWLGYGHSVTENLALLRQMASPLVRFDSCKLHACASGNSALATAASDGRAHIYQR
CCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEECCCCCEEEEEC
DGLLAALWGRGNFLEPRLDEQAQREGMAKTLAENWRGRSGGDGRGAKIFESLSGAFACCI
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEE
VDENANEVVLATDRMGTYPLSYQALAGGLVFASSSDAIRLHPSIRSEIDLQSLYNYVYFH
EECCCCEEEEEECCCCCCCCCHHHHHCCEEEECCCCCEEECCCHHHHCCHHHHHHHHEEE
MVPSPDTIYEGQKRLMPGEYLVYSKGGIKTEKYWLMRFQEDERHPFQELKQEFLEVLHSS
ECCCCCHHHCCHHHCCCCCEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
VRTAIGDDETGAFLSGGTDSSTIAGILGEVSGQPARTYSIGFDATGYDEMGYARIAARHF
HHHHCCCCCCCCEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHH
GTRHQEYYVTPENIVSAIPQVAAVFDQPFGNSSALPAYYCAQMAKSDGLSRMLGGDGGDE
CCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCC
LFGGNVRYAKQHIFSLYEQTPLLLRKGLIEPVVFGLPGGGALPLIRKARSYVEQASIPMP
CCCCCHHHHHHHHHHHHHCCHHHHHHCCHHHEEEECCCCCCHHHHHHHHHHHHHCCCCCC
ARTETYNLLERYGRDAIFTPEFLDAVNPGYPARLLDETYHENDAKSLINQMLAFDRKFTL
CCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEE
ADNDLPKVAKACELAGMDVAFPLISDEIVAFSLRLEPHLKLKGTKLRYFFKEALRGFLPD
CCCCHHHHHHHHHHCCCCEEHHHHCCCEEEEEEEECCCEEECCCHHHHHHHHHHHCCCCH
DIIAKQKHGFGLPFGVWLQTHKNLQDLAWDSLSDLKSRNIIRGDFIEKLLQQHLEEHAGY
HHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
HGTMVWVLMMLEQWYRQRQDSRSAMHSQA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SGLCGWLGYGHSVTENLALLRQMASPLVRFDSCKLHACASGNSALATAASDGRAHIYQR
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEEECCCCCEEEEEC
DGLLAALWGRGNFLEPRLDEQAQREGMAKTLAENWRGRSGGDGRGAKIFESLSGAFACCI
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCEEEEE
VDENANEVVLATDRMGTYPLSYQALAGGLVFASSSDAIRLHPSIRSEIDLQSLYNYVYFH
EECCCCEEEEEECCCCCCCCCHHHHHCCEEEECCCCCEEECCCHHHHCCHHHHHHHHEEE
MVPSPDTIYEGQKRLMPGEYLVYSKGGIKTEKYWLMRFQEDERHPFQELKQEFLEVLHSS
ECCCCCHHHCCHHHCCCCCEEEECCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
VRTAIGDDETGAFLSGGTDSSTIAGILGEVSGQPARTYSIGFDATGYDEMGYARIAARHF
HHHHCCCCCCCCEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHH
GTRHQEYYVTPENIVSAIPQVAAVFDQPFGNSSALPAYYCAQMAKSDGLSRMLGGDGGDE
CCCCCEEEECHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCCCCC
LFGGNVRYAKQHIFSLYEQTPLLLRKGLIEPVVFGLPGGGALPLIRKARSYVEQASIPMP
CCCCCHHHHHHHHHHHHHCCHHHHHHCCHHHEEEECCCCCCHHHHHHHHHHHHHCCCCCC
ARTETYNLLERYGRDAIFTPEFLDAVNPGYPARLLDETYHENDAKSLINQMLAFDRKFTL
CCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCEEE
ADNDLPKVAKACELAGMDVAFPLISDEIVAFSLRLEPHLKLKGTKLRYFFKEALRGFLPD
CCCCHHHHHHHHHHCCCCEEHHHHCCCEEEEEEEECCCEEECCCHHHHHHHHHHHCCCCH
DIIAKQKHGFGLPFGVWLQTHKNLQDLAWDSLSDLKSRNIIRGDFIEKLLQQHLEEHAGY
HHHHHCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCC
HGTMVWVLMMLEQWYRQRQDSRSAMHSQA
CHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]