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The map label for this gene is prfA

Identifier: 82703754

GI number: 82703754

Start: 3026322

End: 3027398

Strand: Reverse

Name: prfA

Synonym: Nmul_A2641

Alternate gene names: 82703754

Gene position: 3027398-3026322 (Counterclockwise)

Preceding gene: 82703755

Following gene: 82703753

Centisome position: 95.07

GC content: 57.75

Gene sequence:

>1077_bases
ATGAACAAAAGTATGACTGCCAAGCTCACGCAACTCAGCGTGCGCCTGGAGGAGTTGAATCGTCTGCTGAGCAGCGAAAG
CATCACGGTCAATCTCGATCAATACCGCAAGCTGACGCGCGAACGTGCGGAGATTGCCCCCGTGGTCGACCTGTATAACG
CTTATCTGCAAAGCGAGCAGGATATTCATACCGCGCAGGAGATGGCTGCCGAGGCGGAAATGCGCGAGTTTGCCGATGCC
GAGATACGGGATGCCAAAGAGAGGCTGGTGCGTTATGGCGCGGAATTGCAGAAGCAATTGCTGCCCAAAGACCCGAACGA
TGAGCGCAATATTTTTCTGGAGATCCGGGCCGGTACCGGAGGAGACGAGTCCGCGCTGTTTGCGGCGGACCTGTTCCGCA
TGTATGCACGCTTTGCTGAACGCCAGCGCTGGCAGGTGGAAATCATTTCGCAAAGCCCGTCCGACGTCGGCGGATATAAG
GAAATCATTGCCAAGATCAGCGGTGAAGGCGCCTATTCCAAACTCAAGTTTGAATCGGGTGGGCACCGGGTGCAGCGCGT
GCCAGCGACCGAAACGCAGGGACGTATCCATACTTCCGCTTGCACCGTCGCGGTGATGCCGGAGGCAGACGAGATCGAGG
ATGTCGCGCTCAATCCTGCCGAGCTGAGGATCGATACTTTCCGTGCTTCCGGGGCGGGAGGCCAGCATATCAACAAGACT
GATTCTGCCGTGCGCATCACTCACCTGCCGACGGGAATCGTGGTCGAATGCCAGGATGGCCGTTCCCAACATAAAAACAA
GGCGCAGGCGATGAGCGTGCTGGCTGCGCGCATCCGCGACAAGCAGATGCAGGAGCAGCAAAGCAAACAGGCGGCAACGC
GCAAGTCGCTGGTAGGCACGGGTAATCGTTCAGGACGTATCCGCACTTACAATTTTCCCCAGGGGCGGATAACGGATCAC
CGCATCAATCTGACGCTGTACAAGATCGAGCAGATCATGGATGGGGATTTGAACGAGCTTTGCTCGGCCCTGCTGGCCGA
GCATCAGGCCGAGCAGTTGGCGGCAATGGCGGAGTAG

Upstream 100 bases:

>100_bases
TCCTCCATTTACCCTCCTATGCGCTCAACCACGCCGCTGAGGACGAGCGTGACGATCTGGTCGACCTGATCAGCCGCCTG
TATCACCTTCATCCCTCAGA

Downstream 100 bases:

>100_bases
ATTACCGGCCGGTGTCGTCCCTCACCATTTTCGAAGCGCTGGGACAAGCCCGCCGGAACATTGCCGATATCGACGCGCGG
ATGCTGTTGCAGTACGTGCT

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MNKSMTAKLTQLSVRLEELNRLLSSESITVNLDQYRKLTRERAEIAPVVDLYNAYLQSEQDIHTAQEMAAEAEMREFADA
EIRDAKERLVRYGAELQKQLLPKDPNDERNIFLEIRAGTGGDESALFAADLFRMYARFAERQRWQVEIISQSPSDVGGYK
EIIAKISGEGAYSKLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEIEDVALNPAELRIDTFRASGAGGQHINKT
DSAVRITHLPTGIVVECQDGRSQHKNKAQAMSVLAARIRDKQMQEQQSKQAATRKSLVGTGNRSGRIRTYNFPQGRITDH
RINLTLYKIEQIMDGDLNELCSALLAEHQAEQLAAMAE

Sequences:

>Translated_358_residues
MNKSMTAKLTQLSVRLEELNRLLSSESITVNLDQYRKLTRERAEIAPVVDLYNAYLQSEQDIHTAQEMAAEAEMREFADA
EIRDAKERLVRYGAELQKQLLPKDPNDERNIFLEIRAGTGGDESALFAADLFRMYARFAERQRWQVEIISQSPSDVGGYK
EIIAKISGEGAYSKLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEIEDVALNPAELRIDTFRASGAGGQHINKT
DSAVRITHLPTGIVVECQDGRSQHKNKAQAMSVLAARIRDKQMQEQQSKQAATRKSLVGTGNRSGRIRTYNFPQGRITDH
RINLTLYKIEQIMDGDLNELCSALLAEHQAEQLAAMAE
>Mature_358_residues
MNKSMTAKLTQLSVRLEELNRLLSSESITVNLDQYRKLTRERAEIAPVVDLYNAYLQSEQDIHTAQEMAAEAEMREFADA
EIRDAKERLVRYGAELQKQLLPKDPNDERNIFLEIRAGTGGDESALFAADLFRMYARFAERQRWQVEIISQSPSDVGGYK
EIIAKISGEGAYSKLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEIEDVALNPAELRIDTFRASGAGGQHINKT
DSAVRITHLPTGIVVECQDGRSQHKNKAQAMSVLAARIRDKQMQEQQSKQAATRKSLVGTGNRSGRIRTYNFPQGRITDH
RINLTLYKIEQIMDGDLNELCSALLAEHQAEQLAAMAE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=307, Percent_Identity=45.2768729641694, Blast_Score=278, Evalue=4e-75,
Organism=Homo sapiens, GI34577120, Length=370, Percent_Identity=40, Blast_Score=264, Evalue=1e-70,
Organism=Homo sapiens, GI166795305, Length=212, Percent_Identity=43.3962264150943, Blast_Score=187, Evalue=2e-47,
Organism=Escherichia coli, GI1787462, Length=358, Percent_Identity=61.4525139664804, Blast_Score=453, Evalue=1e-129,
Organism=Escherichia coli, GI2367172, Length=336, Percent_Identity=39.2857142857143, Blast_Score=229, Evalue=3e-61,
Organism=Caenorhabditis elegans, GI17542784, Length=264, Percent_Identity=36.7424242424242, Blast_Score=179, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6321295, Length=361, Percent_Identity=38.781163434903, Blast_Score=239, Evalue=5e-64,
Organism=Drosophila melanogaster, GI19921226, Length=301, Percent_Identity=41.8604651162791, Blast_Score=219, Evalue=2e-57,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_NITMU (Q2Y5P3)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_413320.1
- HSSP:   P0A7I0
- ProteinModelPortal:   Q2Y5P3
- SMR:   Q2Y5P3
- STRING:   Q2Y5P3
- GeneID:   3785252
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2641
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   Q2Y5P3
- ProtClustDB:   PRK00591
- BioCyc:   NMUL323848:NMUL_A2641-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 40074; Mature: 40074

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKSMTAKLTQLSVRLEELNRLLSSESITVNLDQYRKLTRERAEIAPVVDLYNAYLQSEQ
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DIHTAQEMAAEAEMREFADAEIRDAKERLVRYGAELQKQLLPKDPNDERNIFLEIRAGTG
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCC
GDESALFAADLFRMYARFAERQRWQVEIISQSPSDVGGYKEIIAKISGEGAYSKLKFESG
CCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEECCC
GHRVQRVPATETQGRIHTSACTVAVMPEADEIEDVALNPAELRIDTFRASGAGGQHINKT
CCEEEECCCCCCCCEEEECEEEEEECCCCCCHHHHCCCHHHEEEEEEECCCCCCCCCCCC
DSAVRITHLPTGIVVECQDGRSQHKNKAQAMSVLAARIRDKQMQEQQSKQAATRKSLVGT
CCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GNRSGRIRTYNFPQGRITDHRINLTLYKIEQIMDGDLNELCSALLAEHQAEQLAAMAE
CCCCCCEEEEECCCCCCCCEEEEEEHEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNKSMTAKLTQLSVRLEELNRLLSSESITVNLDQYRKLTRERAEIAPVVDLYNAYLQSEQ
CCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DIHTAQEMAAEAEMREFADAEIRDAKERLVRYGAELQKQLLPKDPNDERNIFLEIRAGTG
HHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCC
GDESALFAADLFRMYARFAERQRWQVEIISQSPSDVGGYKEIIAKISGEGAYSKLKFESG
CCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEEECCC
GHRVQRVPATETQGRIHTSACTVAVMPEADEIEDVALNPAELRIDTFRASGAGGQHINKT
CCEEEECCCCCCCCEEEECEEEEEECCCCCCHHHHCCCHHHEEEEEEECCCCCCCCCCCC
DSAVRITHLPTGIVVECQDGRSQHKNKAQAMSVLAARIRDKQMQEQQSKQAATRKSLVGT
CCEEEEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GNRSGRIRTYNFPQGRITDHRINLTLYKIEQIMDGDLNELCSALLAEHQAEQLAAMAE
CCCCCCEEEEECCCCCCCCEEEEEEHEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA