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The map label for this gene is thiDE [H]

Identifier: 82703740

GI number: 82703740

Start: 3010319

End: 3011158

Strand: Direct

Name: thiDE [H]

Synonym: Nmul_A2627

Alternate gene names: 82703740

Gene position: 3010319-3011158 (Clockwise)

Preceding gene: 82703736

Following gene: 82703741

Centisome position: 94.54

GC content: 56.9

Gene sequence:

>840_bases
ATGTCTCAGCCACCCCCCATCGTACTTTCTTTTGCTGCCAGCGACTCTACCGGGGGCGCCGGTATCCAGGCTGATATTCT
CACTCTGGCCAGCATGGGATGTCACCCCCTTTCGGTCATCACCGCCGTGACGGTGCAGGATACGGCGGGAGTAGATGATG
TAATGGCCCTGGATGCAGAATGGGTGACGGATCAGGCACGCGCGGTTCTTGAGGACATGCCCGTGCATGTGTTCAAAATG
GGTTTGCTGGGCAGTGTGGAAATTATTGCGGCGATCGCCGAAGTGGTTTCGGATTATCCCAACATTCCAGTCGTGCTGGA
CCCTGTGCTTGCCTCGGGCCGGGGCGATGAACTCGCCAATGAGGAAATGGTGGCTGCCATGCGGGAATTGCTGCTGCCGC
AGGCAACCATCATCACGCCCAACAGCCTCGAAGCGCGGCGATTGGCGCAGGATTATGAGGATGAGTCCGATATGCCGGAT
TTGCACCAGTGCGCAGGCCGATTGCTGCGCCTGGGATGTGAATATGTATTCGTTACCGGAACCCATGAAAATACTTCGCA
AGTGGTGAACACGCTATACGGAACTACCGGCATCGTGCGCACGGATGCATGGAATCGCCTGACGGGCTCGTATCATGGAT
CGGGGTGCACACTCGCCTCTGCTATCGCCGCCACGCTTGCCAATGGATTGCCTATTGCAGAGGGTGTATATGAAGCGCAG
GAATACACCTGGCAATCGCTGAAAGCCGGGTTCAGGCCTGGAATGGGGCAACATCTGCCAGACAGGCTATTCTGGGCGCG
TGATGAGAACGGTGTCGAGGAACGAAATCAAGCGATGTAG

Upstream 100 bases:

>100_bases
GGCACATATAGCAACGGTAGACACGCACTTCGGTCGTTTCGGTGGTAGTCATGATGAGTAGGGGTATTTAGGTTAAAATA
TCATTTTACGGTATTAGTTC

Downstream 100 bases:

>100_bases
CGCTGCTCCGCGTCGAGAAATAACCGGTCTATATGCGATCACACCAGATACAGGCGATACATCGCATCTGGTCGCGATGA
CATGGAGGGCGCTTGCCGGA

Product: phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase [H]

Number of amino acids: Translated: 279; Mature: 278

Protein sequence:

>279_residues
MSQPPPIVLSFAASDSTGGAGIQADILTLASMGCHPLSVITAVTVQDTAGVDDVMALDAEWVTDQARAVLEDMPVHVFKM
GLLGSVEIIAAIAEVVSDYPNIPVVLDPVLASGRGDELANEEMVAAMRELLLPQATIITPNSLEARRLAQDYEDESDMPD
LHQCAGRLLRLGCEYVFVTGTHENTSQVVNTLYGTTGIVRTDAWNRLTGSYHGSGCTLASAIAATLANGLPIAEGVYEAQ
EYTWQSLKAGFRPGMGQHLPDRLFWARDENGVEERNQAM

Sequences:

>Translated_279_residues
MSQPPPIVLSFAASDSTGGAGIQADILTLASMGCHPLSVITAVTVQDTAGVDDVMALDAEWVTDQARAVLEDMPVHVFKM
GLLGSVEIIAAIAEVVSDYPNIPVVLDPVLASGRGDELANEEMVAAMRELLLPQATIITPNSLEARRLAQDYEDESDMPD
LHQCAGRLLRLGCEYVFVTGTHENTSQVVNTLYGTTGIVRTDAWNRLTGSYHGSGCTLASAIAATLANGLPIAEGVYEAQ
EYTWQSLKAGFRPGMGQHLPDRLFWARDENGVEERNQAM
>Mature_278_residues
SQPPPIVLSFAASDSTGGAGIQADILTLASMGCHPLSVITAVTVQDTAGVDDVMALDAEWVTDQARAVLEDMPVHVFKMG
LLGSVEIIAAIAEVVSDYPNIPVVLDPVLASGRGDELANEEMVAAMRELLLPQATIITPNSLEARRLAQDYEDESDMPDL
HQCAGRLLRLGCEYVFVTGTHENTSQVVNTLYGTTGIVRTDAWNRLTGSYHGSGCTLASAIAATLANGLPIAEGVYEAQE
YTWQSLKAGFRPGMGQHLPDRLFWARDENGVEERNQAM

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P [H]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiD family [H]

Homologues:

Organism=Escherichia coli, GI1788420, Length=235, Percent_Identity=37.0212765957447, Blast_Score=138, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6325378, Length=255, Percent_Identity=30.9803921568627, Blast_Score=110, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6324517, Length=256, Percent_Identity=30.46875, Blast_Score=101, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324997, Length=254, Percent_Identity=32.2834645669291, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF08543 Phos_pyr_kin; PF02581 TMP-TENI [H]

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7 [H]

Molecular weight: Translated: 29915; Mature: 29784

Theoretical pI: Translated: 4.13; Mature: 4.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQPPPIVLSFAASDSTGGAGIQADILTLASMGCHPLSVITAVTVQDTAGVDDVMALDAE
CCCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHEEEEECCCCCCHHHHHHCHH
WVTDQARAVLEDMPVHVFKMGLLGSVEIIAAIAEVVSDYPNIPVVLDPVLASGRGDELAN
HHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECHHHHCCCCCCHHH
EEMVAAMRELLLPQATIITPNSLEARRLAQDYEDESDMPDLHQCAGRLLRLGCEYVFVTG
HHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEC
THENTSQVVNTLYGTTGIVRTDAWNRLTGSYHGSGCTLASAIAATLANGLPIAEGVYEAQ
CCCCHHHHHHHHHCCCCEEECCCCHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
EYTWQSLKAGFRPGMGQHLPDRLFWARDENGVEERNQAM
HHHHHHHHHCCCCCCCCCCCHHHEECCCCCCHHHHHCCC
>Mature Secondary Structure 
SQPPPIVLSFAASDSTGGAGIQADILTLASMGCHPLSVITAVTVQDTAGVDDVMALDAE
CCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCHHHHHEEEEECCCCCCHHHHHHCHH
WVTDQARAVLEDMPVHVFKMGLLGSVEIIAAIAEVVSDYPNIPVVLDPVLASGRGDELAN
HHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEECHHHHCCCCCCHHH
EEMVAAMRELLLPQATIITPNSLEARRLAQDYEDESDMPDLHQCAGRLLRLGCEYVFVTG
HHHHHHHHHHHCCCCEEECCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCEEEEEC
THENTSQVVNTLYGTTGIVRTDAWNRLTGSYHGSGCTLASAIAATLANGLPIAEGVYEAQ
CCCCHHHHHHHHHCCCCEEECCCCHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHH
EYTWQSLKAGFRPGMGQHLPDRLFWARDENGVEERNQAM
HHHHHHHHHCCCCCCCCCCCHHHEECCCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA