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The map label for this gene is purN [H]

Identifier: 82703731

GI number: 82703731

Start: 3003570

End: 3004208

Strand: Direct

Name: purN [H]

Synonym: Nmul_A2618

Alternate gene names: 82703731

Gene position: 3003570-3004208 (Clockwise)

Preceding gene: 82703730

Following gene: 82703732

Centisome position: 94.33

GC content: 55.87

Gene sequence:

>639_bases
ATGAAGTCACTCGTGATCCTTATCTCCGGCCGCGGCAGCAATATGCAAGCTCTTATGGAAGCAAATCTCCCTGCCCGGAT
AGCCGCGGTCATCAGCAACAAGCCAGAAGCGCCAGGGCTCGAAACCGCGCGCAGCCGCGGTTATGAAACCATTGTGCTGG
ACCCACGGTCATACCCGGATCGCGAAGCGTTTGACCAGAAACTTGCCGAGGCGATCGATGCCTACGCCCCCGATCTCGTC
GCCCTGGCCGGTTTCATGCGCCTGCTCGGGGACAATTTTGTCAGCCGATATAAAGGCAGGCTGATAAACATTCATCCTTC
GCTGCTGCCCGCTTTTCCCGGCCTGCACCCTCACCGGCAGGCATTGAAGGAAGGCGTGAAGGTGCATGGATGCACAGTGC
ATTTTGTCACCGCCGAAACGGATCGAGGTCCGATCATTATCCAGGCGGCTGTTCAGGTCATGCCGGACGATACTGAGGAA
ACACTGGCCGCGCGCGTGCTGCGACAGGAACATCGCATTTATCCTGAAGCCGTGCGCTGGTTTATGAAAGACCGGTTGAA
GCTCTCTGATAACAGTGTTGAGGTGAGCGATGCCGATTTCGACAATTCTGTTCTATATTCACCCGGACTATCCAAATGA

Upstream 100 bases:

>100_bases
TTGGCACCATTCGCGAGCAGCGCGCGGACGAACCCCGGACGATCCTCGAGTGAGCAGTGTGGAGCACTACACACATGCAA
CGTGAATCCCCTGCTGATCC

Downstream 100 bases:

>100_bases
AATTTTTATTTCTAGCTCTGTTGTCGGGCTCAGTTTTTTTTGCTTTTCCCGCTTTTTCCGCTTGCGCTGCCTGCGGCACA
GAAGTGCCGTCGCGCGTCGA

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MKSLVILISGRGSNMQALMEANLPARIAAVISNKPEAPGLETARSRGYETIVLDPRSYPDREAFDQKLAEAIDAYAPDLV
ALAGFMRLLGDNFVSRYKGRLINIHPSLLPAFPGLHPHRQALKEGVKVHGCTVHFVTAETDRGPIIIQAAVQVMPDDTEE
TLAARVLRQEHRIYPEAVRWFMKDRLKLSDNSVEVSDADFDNSVLYSPGLSK

Sequences:

>Translated_212_residues
MKSLVILISGRGSNMQALMEANLPARIAAVISNKPEAPGLETARSRGYETIVLDPRSYPDREAFDQKLAEAIDAYAPDLV
ALAGFMRLLGDNFVSRYKGRLINIHPSLLPAFPGLHPHRQALKEGVKVHGCTVHFVTAETDRGPIIIQAAVQVMPDDTEE
TLAARVLRQEHRIYPEAVRWFMKDRLKLSDNSVEVSDADFDNSVLYSPGLSK
>Mature_212_residues
MKSLVILISGRGSNMQALMEANLPARIAAVISNKPEAPGLETARSRGYETIVLDPRSYPDREAFDQKLAEAIDAYAPDLV
ALAGFMRLLGDNFVSRYKGRLINIHPSLLPAFPGLHPHRQALKEGVKVHGCTVHFVTAETDRGPIIIQAAVQVMPDDTEE
TLAARVLRQEHRIYPEAVRWFMKDRLKLSDNSVEVSDADFDNSVLYSPGLSK

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=193, Percent_Identity=44.559585492228, Blast_Score=167, Evalue=5e-42,
Organism=Homo sapiens, GI209869995, Length=193, Percent_Identity=44.559585492228, Blast_Score=167, Evalue=5e-42,
Organism=Homo sapiens, GI209869993, Length=193, Percent_Identity=44.559585492228, Blast_Score=167, Evalue=5e-42,
Organism=Escherichia coli, GI1788846, Length=194, Percent_Identity=52.5773195876289, Blast_Score=207, Evalue=5e-55,
Organism=Escherichia coli, GI1787483, Length=165, Percent_Identity=40, Blast_Score=108, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17567511, Length=179, Percent_Identity=44.1340782122905, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320616, Length=204, Percent_Identity=30.3921568627451, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24582400, Length=183, Percent_Identity=45.9016393442623, Blast_Score=168, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 23476; Mature: 23476

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLVILISGRGSNMQALMEANLPARIAAVISNKPEAPGLETARSRGYETIVLDPRSYPD
CCEEEEEEECCCCCHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHCCCEEEEECCCCCCC
REAFDQKLAEAIDAYAPDLVALAGFMRLLGDNFVSRYKGRLINIHPSLLPAFPGLHPHRQ
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHCCCCCCHHHH
ALKEGVKVHGCTVHFVTAETDRGPIIIQAAVQVMPDDTEETLAARVLRQEHRIYPEAVRW
HHHCCCEEECEEEEEEEECCCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHCCCHHHHHH
FMKDRLKLSDNSVEVSDADFDNSVLYSPGLSK
HHHHHHEECCCCEEECCCCCCCCEEECCCCCC
>Mature Secondary Structure
MKSLVILISGRGSNMQALMEANLPARIAAVISNKPEAPGLETARSRGYETIVLDPRSYPD
CCEEEEEEECCCCCHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHCCCEEEEECCCCCCC
REAFDQKLAEAIDAYAPDLVALAGFMRLLGDNFVSRYKGRLINIHPSLLPAFPGLHPHRQ
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHCCCCCCHHHH
ALKEGVKVHGCTVHFVTAETDRGPIIIQAAVQVMPDDTEETLAARVLRQEHRIYPEAVRW
HHHCCCEEECEEEEEEEECCCCCCEEEEEEHHHCCCCHHHHHHHHHHHHHHCCCHHHHHH
FMKDRLKLSDNSVEVSDADFDNSVLYSPGLSK
HHHHHHEECCCCEEECCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]