Click here to switch to the map view.

The map label for this gene is mlaA [H]

Identifier: 82703672

GI number: 82703672

Start: 2926902

End: 2927684

Strand: Reverse

Name: mlaA [H]

Synonym: Nmul_A2557

Alternate gene names: 82703672

Gene position: 2927684-2926902 (Counterclockwise)

Preceding gene: 82703673

Following gene: 82703671

Centisome position: 91.94

GC content: 54.79

Gene sequence:

>783_bases
ATGGTGGCAAATATGCGAACTTCCTTCCTGTTGTTGGCATGCATATCACTGTTTACCGGTTGCGCGGCGCTGCATAAGAA
CGATGCGAGTATCGATACGATCGATCCGAATGAAAGAGCCAATCGACAGTTTTACAATTTTACCGACAAGGTGGATCGCG
CCGTTCTGGCGCCGGTTGCGGATGCTTATATAAAGCATGTGCCCGACCCCCTGCAGCGTTCGATTGGCAATTTCTACGAC
AACCTTGCATATCCCAATGTTATTCTTAATGCTTTCCTGCAAGGTAAAGTACGGCAGGGATTCCAGGACACCCTTCGCTT
TGCGGTCAATTCGACCATCGGCCTGGGCGGGCTCTTCGACATGGCAGGGCCCATGGGGTTGCCACAGCATGATGAAGATT
TCGGGCAGACCCTCGGCGTCTGGGGCGTTGATACCGGCTCCTACCTTTTTGTCCCTCTGATCGGCCCCAGCAGCTATCGG
GATGCGCCCGGCATTCCTGTGAGCGTTTTCAGCAACCTTTTGTTTTATGCGGGTACTGTTGCCAGCAGCGCGGTGGTAGC
TCCAATCACGGTTTTAAGTATCATCGACAGGCGGGCGCGATTGTCCGGCCCCATGCGTATTCGGGACCAGGCCGCTCTGG
ACCCCTATCTTTTCGTTCGCGAAGGTTTTTTACAGCAGCGCAAGCACTTGATCTATGATGGCGACCCGCCGCCCGAAAGC
CTGGACGAATTCGCAGATGAGGATTCCGTACAGAACGAGGCCGCGGCCGAGCCATTGCGATGA

Upstream 100 bases:

>100_bases
ATTGTATACTTCGCACCTGTGGCGGTTTGTGGACATCCCCTTCCATGCGGATTCTGATTCTGAGGGACAGATTAACAGGG
ACATAGCCTTCCCTTTACGG

Downstream 100 bases:

>100_bases
GATTTTAATCAAATCTCAACTGCTGCTTTCACTTGTCCAGCGGGCGCTGCATCTGCCATAAACATCCTTGCATGAATCTC
GACGATGTACCGATTTCGAG

Product: VacJ-like lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MVANMRTSFLLLACISLFTGCAALHKNDASIDTIDPNERANRQFYNFTDKVDRAVLAPVADAYIKHVPDPLQRSIGNFYD
NLAYPNVILNAFLQGKVRQGFQDTLRFAVNSTIGLGGLFDMAGPMGLPQHDEDFGQTLGVWGVDTGSYLFVPLIGPSSYR
DAPGIPVSVFSNLLFYAGTVASSAVVAPITVLSIIDRRARLSGPMRIRDQAALDPYLFVREGFLQQRKHLIYDGDPPPES
LDEFADEDSVQNEAAAEPLR

Sequences:

>Translated_260_residues
MVANMRTSFLLLACISLFTGCAALHKNDASIDTIDPNERANRQFYNFTDKVDRAVLAPVADAYIKHVPDPLQRSIGNFYD
NLAYPNVILNAFLQGKVRQGFQDTLRFAVNSTIGLGGLFDMAGPMGLPQHDEDFGQTLGVWGVDTGSYLFVPLIGPSSYR
DAPGIPVSVFSNLLFYAGTVASSAVVAPITVLSIIDRRARLSGPMRIRDQAALDPYLFVREGFLQQRKHLIYDGDPPPES
LDEFADEDSVQNEAAAEPLR
>Mature_260_residues
MVANMRTSFLLLACISLFTGCAALHKNDASIDTIDPNERANRQFYNFTDKVDRAVLAPVADAYIKHVPDPLQRSIGNFYD
NLAYPNVILNAFLQGKVRQGFQDTLRFAVNSTIGLGGLFDMAGPMGLPQHDEDFGQTLGVWGVDTGSYLFVPLIGPSSYR
DAPGIPVSVFSNLLFYAGTVASSAVVAPITVLSIIDRRARLSGPMRIRDQAALDPYLFVREGFLQQRKHLIYDGDPPPES
LDEFADEDSVQNEAAAEPLR

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=246, Percent_Identity=33.739837398374, Blast_Score=106, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 28530; Mature: 28530

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVANMRTSFLLLACISLFTGCAALHKNDASIDTIDPNERANRQFYNFTDKVDRAVLAPVA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHH
DAYIKHVPDPLQRSIGNFYDNLAYPNVILNAFLQGKVRQGFQDTLRFAVNSTIGLGGLFD
HHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
MAGPMGLPQHDEDFGQTLGVWGVDTGSYLFVPLIGPSSYRDAPGIPVSVFSNLLFYAGTV
HCCCCCCCCCCHHHHHCCCCEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
ASSAVVAPITVLSIIDRRARLSGPMRIRDQAALDPYLFVREGFLQQRKHLIYDGDPPPES
HHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHCCHHHHHHHHHHHHHHCEEECCCCCHHH
LDEFADEDSVQNEAAAEPLR
HHHHHCHHHHHHHHHCCCCC
>Mature Secondary Structure
MVANMRTSFLLLACISLFTGCAALHKNDASIDTIDPNERANRQFYNFTDKVDRAVLAPVA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHH
DAYIKHVPDPLQRSIGNFYDNLAYPNVILNAFLQGKVRQGFQDTLRFAVNSTIGLGGLFD
HHHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
MAGPMGLPQHDEDFGQTLGVWGVDTGSYLFVPLIGPSSYRDAPGIPVSVFSNLLFYAGTV
HCCCCCCCCCCHHHHHCCCCEECCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHH
ASSAVVAPITVLSIIDRRARLSGPMRIRDQAALDPYLFVREGFLQQRKHLIYDGDPPPES
HHHHHHHHHHHHHHHHHHHHHCCCCEECHHHHCCHHHHHHHHHHHHHHCEEECCCCCHHH
LDEFADEDSVQNEAAAEPLR
HHHHHCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]