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The map label for this gene is wza [H]

Identifier: 82703644

GI number: 82703644

Start: 2895812

End: 2896438

Strand: Reverse

Name: wza [H]

Synonym: Nmul_A2529

Alternate gene names: 82703644

Gene position: 2896438-2895812 (Counterclockwise)

Preceding gene: 82703645

Following gene: 82703643

Centisome position: 90.96

GC content: 53.11

Gene sequence:

>627_bases
GTGAACCCCCTTCGTATCCGTACCGCAACATGGTTCTCTTGTTTTGCTGTTGTACTTCCCCTTCTTTCCCTTGGGGGTTG
CAAAACCTACCCCTCCTTGAATCCAGATGAAAAACCCGTTGCACACGATTACCTGATCGGCCCGGGAGATAATGTCGATA
TCATTGTCTGGCGCAATCCCGAAGTTTCCATGGCGGTGCCCGTTCGGCCTGATGGCAAGATTACCACCCCTCTGGTAGAG
GATCTGTCGGCAAGTGGAAAGACCTCGACGCAGCTTGCGCGAGATATCGAGGAGGCATTGTCGAAGTACATCCAGCAACC
GGTCGTGACGGTTGTGGTGACAGAATTTGTAGGTCCATATTCCGAGCAGATCCGGGTGATAGGGGAAGCAGCCGAACCTC
AGGCATTGCCTTATCGGGAGGAGATGACGCTGATGGACGTCCTGATTGCGGTAGGTGGTATGACTGATTTTGCCGCGGGT
AATCGCGCTAGGATAGTACGTAACGTGGATGGGAAACAACATCAGTTCAGCGTGCGGTTGGACGACCTGATCCGGGATGG
GGATGTGACGGCCAACGTACCCATGAAGCAGGGAGATGTACTTGTCATACCGGAAAGTTATTTCTGA

Upstream 100 bases:

>100_bases
TCTTGACAGGTGTATTGACTTATAGCATCTTTATTTTACATTAAAGGTCAATAACCCTCTTCGACTCAGGCATTTTCTAA
TCAACAAAAAAGGAAATTCG

Downstream 100 bases:

>100_bases
TCGAGCGAATTCCACTGCGGCGGCTTAATCTTCCTGTAATGGCATTTCGATAAGGTTTTCATTGCCCGGTAAGTTGAAAT
GAAGAAGAAGAAAATCATTC

Product: polysaccharide export protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MNPLRIRTATWFSCFAVVLPLLSLGGCKTYPSLNPDEKPVAHDYLIGPGDNVDIIVWRNPEVSMAVPVRPDGKITTPLVE
DLSASGKTSTQLARDIEEALSKYIQQPVVTVVVTEFVGPYSEQIRVIGEAAEPQALPYREEMTLMDVLIAVGGMTDFAAG
NRARIVRNVDGKQHQFSVRLDDLIRDGDVTANVPMKQGDVLVIPESYF

Sequences:

>Translated_208_residues
MNPLRIRTATWFSCFAVVLPLLSLGGCKTYPSLNPDEKPVAHDYLIGPGDNVDIIVWRNPEVSMAVPVRPDGKITTPLVE
DLSASGKTSTQLARDIEEALSKYIQQPVVTVVVTEFVGPYSEQIRVIGEAAEPQALPYREEMTLMDVLIAVGGMTDFAAG
NRARIVRNVDGKQHQFSVRLDDLIRDGDVTANVPMKQGDVLVIPESYF
>Mature_208_residues
MNPLRIRTATWFSCFAVVLPLLSLGGCKTYPSLNPDEKPVAHDYLIGPGDNVDIIVWRNPEVSMAVPVRPDGKITTPLVE
DLSASGKTSTQLARDIEEALSKYIQQPVVTVVVTEFVGPYSEQIRVIGEAAEPQALPYREEMTLMDVLIAVGGMTDFAAG
NRARIVRNVDGKQHQFSVRLDDLIRDGDVTANVPMKQGDVLVIPESYF

Specific function: Probably involved in the export of the extracellular polysaccharide colanic acid from the cell to medium [H]

COG id: COG1596

COG function: function code M; Periplasmic protein involved in polysaccharide export

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BexD/CtrA/VexA family [H]

Homologues:

Organism=Escherichia coli, GI1788376, Length=188, Percent_Identity=26.5957446808511, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787218, Length=187, Percent_Identity=31.0160427807487, Blast_Score=67, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003715
- InterPro:   IPR019554 [H]

Pfam domain/function: PF02563 Poly_export; PF10531 SLBB [H]

EC number: NA

Molecular weight: Translated: 22854; Mature: 22854

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPLRIRTATWFSCFAVVLPLLSLGGCKTYPSLNPDEKPVAHDYLIGPGDNVDIIVWRNP
CCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEECCC
EVSMAVPVRPDGKITTPLVEDLSASGKTSTQLARDIEEALSKYIQQPVVTVVVTEFVGPY
CEEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCH
SEQIRVIGEAAEPQALPYREEMTLMDVLIAVGGMTDFAAGNRARIVRNVDGKQHQFSVRL
HHHHEEECCCCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCEEEEECCCCCCEEEEEEH
DDLIRDGDVTANVPMKQGDVLVIPESYF
HHHHCCCCCEECCCCCCCCEEEECCCCC
>Mature Secondary Structure
MNPLRIRTATWFSCFAVVLPLLSLGGCKTYPSLNPDEKPVAHDYLIGPGDNVDIIVWRNP
CCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCEEEEEECCC
EVSMAVPVRPDGKITTPLVEDLSASGKTSTQLARDIEEALSKYIQQPVVTVVVTEFVGPY
CEEEEEECCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCH
SEQIRVIGEAAEPQALPYREEMTLMDVLIAVGGMTDFAAGNRARIVRNVDGKQHQFSVRL
HHHHEEECCCCCCCCCCCHHHHHHHHHHHHHCCCHHHCCCCCEEEEECCCCCCEEEEEEH
DDLIRDGDVTANVPMKQGDVLVIPESYF
HHHHCCCCCEECCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]