Click here to switch to the map view.

The map label for this gene is mraW

Identifier: 82703617

GI number: 82703617

Start: 2859176

End: 2860126

Strand: Reverse

Name: mraW

Synonym: Nmul_A2502

Alternate gene names: 82703617

Gene position: 2860126-2859176 (Counterclockwise)

Preceding gene: 82703618

Following gene: 82703616

Centisome position: 89.82

GC content: 57.52

Gene sequence:

>951_bases
GTGGCTGAGGCAGGAAAGCACACTACTGTTCTGCTACAGGAGGCGGTGGAAGCGCTGGCACTCAAGCCGGATGGGATATA
TGTGGATGGAACATTTGGGCGGGGCGGGCACAGCCGGCTGATACTGGAGCGCCTCGGAAAGAACGGAAGATTGATCGCGT
TCGATAGGGATCCGGTCGCTTGTGCGGTTGGACGGAGTTTTGGAGACGAACGCTTCTGCATGGTTCACAGCAGTTATTCC
AGGTTGCAGGAGGTGCTGCGGCAGTTGAACATCGGCCAGATCGACGGAGTATTACTGGATCTGGGCGTGTCGTCACCCCA
ACTCGAGGAAGCCACACGCGGTTTCAGTTTCAGCATGGAGGGACCGCTCGATATGCGCATGGATACAACAGAAGGGCAGA
CAGCAGCTCAGTGGTTGGCTTCGGCGTCGGAAGTCCAGTTGGAAGAGGTGATAAAAGAATATGGCGAAGAGCGGTTTGCT
AAGCAGATTGCAAGAGCGATTGTCAAGGCTAGAGCGCGACAGCCTCTCGTCACTACCAGTCAACTTGCCGCGATCGTGGC
AGCGGCCATACCCGCGCGCGCGCGTGAGCCAAAACAGAATCCGGCGACGCGTACCTTTCAGGCCATCCGGATTTACCTCA
ATCAGGAGCTTGAGAGGCTGTCGCTTGCCTTACCGCAGTGTGTCGAGATGCTGAAGACGGGTGGCCGGTTGGTCGTCATC
AGTTTTCACTCGCTGGAAGATAGAATAGTGAAGCGTTTCATACGTGAAGGAGCGAATACCGACAAACTGCCTAAGCACTT
GCCGCTGCGCGCCGACGAGGTACGACGTTTCAGCAGGGCAAGTCTGCAGATGGTTGGGAAGGCGGTACGCCCCGGCGTGG
CGGAGGTCGAATGCAATCCTCGCGCGCGCAGCGCAGTGATGCGCGTGGCGGAGCGGATAAACACCACTTAG

Upstream 100 bases:

>100_bases
CGAGCTATGGGACAGCGAGAAATGGAATTTGCAGATGGAGAGCGCGCTCGCGTTCAGAGACGGCATTCCCCAGGAACTCG
AAGGTTTTTCACTGTAATTT

Downstream 100 bases:

>100_bases
CCGAATTCCGTTGCGCGTTTTTGGTTGATGAGAGGTTGGTGTGATCAGAATAAATATTTTGTTGACCTTAATTCTCATCG
CTTGCGCGCTGAGTGTTGTG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MAEAGKHTTVLLQEAVEALALKPDGIYVDGTFGRGGHSRLILERLGKNGRLIAFDRDPVACAVGRSFGDERFCMVHSSYS
RLQEVLRQLNIGQIDGVLLDLGVSSPQLEEATRGFSFSMEGPLDMRMDTTEGQTAAQWLASASEVQLEEVIKEYGEERFA
KQIARAIVKARARQPLVTTSQLAAIVAAAIPARAREPKQNPATRTFQAIRIYLNQELERLSLALPQCVEMLKTGGRLVVI
SFHSLEDRIVKRFIREGANTDKLPKHLPLRADEVRRFSRASLQMVGKAVRPGVAEVECNPRARSAVMRVAERINTT

Sequences:

>Translated_316_residues
MAEAGKHTTVLLQEAVEALALKPDGIYVDGTFGRGGHSRLILERLGKNGRLIAFDRDPVACAVGRSFGDERFCMVHSSYS
RLQEVLRQLNIGQIDGVLLDLGVSSPQLEEATRGFSFSMEGPLDMRMDTTEGQTAAQWLASASEVQLEEVIKEYGEERFA
KQIARAIVKARARQPLVTTSQLAAIVAAAIPARAREPKQNPATRTFQAIRIYLNQELERLSLALPQCVEMLKTGGRLVVI
SFHSLEDRIVKRFIREGANTDKLPKHLPLRADEVRRFSRASLQMVGKAVRPGVAEVECNPRARSAVMRVAERINTT
>Mature_315_residues
AEAGKHTTVLLQEAVEALALKPDGIYVDGTFGRGGHSRLILERLGKNGRLIAFDRDPVACAVGRSFGDERFCMVHSSYSR
LQEVLRQLNIGQIDGVLLDLGVSSPQLEEATRGFSFSMEGPLDMRMDTTEGQTAAQWLASASEVQLEEVIKEYGEERFAK
QIARAIVKARARQPLVTTSQLAAIVAAAIPARAREPKQNPATRTFQAIRIYLNQELERLSLALPQCVEMLKTGGRLVVIS
FHSLEDRIVKRFIREGANTDKLPKHLPLRADEVRRFSRASLQMVGKAVRPGVAEVECNPRARSAVMRVAERINTT

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family

Homologues:

Organism=Homo sapiens, GI165377209, Length=337, Percent_Identity=38.2789317507418, Blast_Score=192, Evalue=4e-49,
Organism=Homo sapiens, GI165377202, Length=189, Percent_Identity=39.1534391534392, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1786270, Length=315, Percent_Identity=55.8730158730159, Blast_Score=355, Evalue=2e-99,
Organism=Drosophila melanogaster, GI62472493, Length=332, Percent_Identity=38.855421686747, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI28571637, Length=332, Percent_Identity=38.855421686747, Blast_Score=196, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMH_NITMU (Q2Y630)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_413183.1
- ProteinModelPortal:   Q2Y630
- SMR:   Q2Y630
- STRING:   Q2Y630
- GeneID:   3786761
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2502
- eggNOG:   COG0275
- HOGENOM:   HBG302779
- OMA:   PQLDDPE
- PhylomeDB:   Q2Y630
- ProtClustDB:   PRK00050
- BioCyc:   NMUL323848:NMUL_A2502-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01007
- InterPro:   IPR002903
- PANTHER:   PTHR11265
- PIRSF:   PIRSF004486
- TIGRFAMs:   TIGR00006

Pfam domain/function: PF01795 Methyltransf_5

EC number: NA

Molecular weight: Translated: 34974; Mature: 34843

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: BINDING 55-55 BINDING 79-79 BINDING 100-100 BINDING 107-107

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAGKHTTVLLQEAVEALALKPDGIYVDGTFGRGGHSRLILERLGKNGRLIAFDRDPVA
CCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCEEEECCCCCE
CAVGRSFGDERFCMVHSSYSRLQEVLRQLNIGQIDGVLLDLGVSSPQLEEATRGFSFSME
EECCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHCCCCEECC
GPLDMRMDTTEGQTAAQWLASASEVQLEEVIKEYGEERFAKQIARAIVKARARQPLVTTS
CCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
QLAAIVAAAIPARAREPKQNPATRTFQAIRIYLNQELERLSLALPQCVEMLKTGGRLVVI
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
SFHSLEDRIVKRFIREGANTDKLPKHLPLRADEVRRFSRASLQMVGKAVRPGVAEVECNP
EEHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
RARSAVMRVAERINTT
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AEAGKHTTVLLQEAVEALALKPDGIYVDGTFGRGGHSRLILERLGKNGRLIAFDRDPVA
CCCCCHHHHHHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCEEEECCCCCE
CAVGRSFGDERFCMVHSSYSRLQEVLRQLNIGQIDGVLLDLGVSSPQLEEATRGFSFSME
EECCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHCCCCEECC
GPLDMRMDTTEGQTAAQWLASASEVQLEEVIKEYGEERFAKQIARAIVKARARQPLVTTS
CCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
QLAAIVAAAIPARAREPKQNPATRTFQAIRIYLNQELERLSLALPQCVEMLKTGGRLVVI
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
SFHSLEDRIVKRFIREGANTDKLPKHLPLRADEVRRFSRASLQMVGKAVRPGVAEVECNP
EEHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCC
RARSAVMRVAERINTT
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA