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The map label for this gene is estA [H]

Identifier: 82703527

GI number: 82703527

Start: 2748633

End: 2749316

Strand: Reverse

Name: estA [H]

Synonym: Nmul_A2412

Alternate gene names: 82703527

Gene position: 2749316-2748633 (Counterclockwise)

Preceding gene: 82703529

Following gene: 82703526

Centisome position: 86.34

GC content: 58.63

Gene sequence:

>684_bases
ATGATCTCCGGTTCCCCCGATCTGCTTCCTTCCATCGAGCTGGAAACGGCCGCTCATCCCACCCGTACGATCATCTGGAT
GCATGGCCTGGGGGCCGATGGCAGTGATTTCGTACCTGTTGTGGATGAGCTTGCGCTTCCTTCCATCCCGGCCGTCCGCT
TCGTGTTTCCCCATGCCCCCACGAGACCCGTCAGCATCAACCGGGGAATGGTCATGCGCGCCTGGTATGACTACGATATT
GTCGATGGCGCGAAACTTCAGGAAAATATGGCAACTCTGCGAGAATCGGAGCGGGCCGTCGAAGCCTTGGTGAACCATGA
AACGCAGCGAGGGGTCAAGCCGGAGAATATCGTTCTGGCAGGCTTCTCTCAGGGGGGTGCCCTGGCGCTGTTCGCCGGTT
TGCGTTATCCGGAAAAACTGGCGGGAATCATGGCGCTTTCATGTTATCTGCCAGCGCCGCAGACCCTTGCGGAAGAGGCA
CACTCTGCCAATTTCGGCATACCAATCTTCATGGCGCACGGGGTGGGCGACAACGTCATCCCTATAACCCTGGCTGCTGC
ATCGCGCCAACAATTGCTCGGAACGGGCTATCCGGTGGAATGGCGCGAGTACGGAATGGCACATACCGTCTGCAGGGAAG
AGATACACGATATCCGCAACTGGCTGCAGCGCGTGCTGGCCTGA

Upstream 100 bases:

>100_bases
ATAGTACTCTCCCTCCCATGACCTTACACATCAGAATCGAAAAGTACGAATCTAATCCCGTGTTTCATTCAAGTCAACGG
GTCGATCTTCATTTTCTCTT

Downstream 100 bases:

>100_bases
GTGGATTGCTTTTCATGATCCATCCAGTACAGCAAGTGCAATTGCAGGCCACGTCTTCTGGCTTTATCCTCCGGATGCTT
CGACGACCAGGTACCACGCC

Product: phospholipase/carboxylesterase

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MISGSPDLLPSIELETAAHPTRTIIWMHGLGADGSDFVPVVDELALPSIPAVRFVFPHAPTRPVSINRGMVMRAWYDYDI
VDGAKLQENMATLRESERAVEALVNHETQRGVKPENIVLAGFSQGGALALFAGLRYPEKLAGIMALSCYLPAPQTLAEEA
HSANFGIPIFMAHGVGDNVIPITLAAASRQQLLGTGYPVEWREYGMAHTVCREEIHDIRNWLQRVLA

Sequences:

>Translated_227_residues
MISGSPDLLPSIELETAAHPTRTIIWMHGLGADGSDFVPVVDELALPSIPAVRFVFPHAPTRPVSINRGMVMRAWYDYDI
VDGAKLQENMATLRESERAVEALVNHETQRGVKPENIVLAGFSQGGALALFAGLRYPEKLAGIMALSCYLPAPQTLAEEA
HSANFGIPIFMAHGVGDNVIPITLAAASRQQLLGTGYPVEWREYGMAHTVCREEIHDIRNWLQRVLA
>Mature_227_residues
MISGSPDLLPSIELETAAHPTRTIIWMHGLGADGSDFVPVVDELALPSIPAVRFVFPHAPTRPVSINRGMVMRAWYDYDI
VDGAKLQENMATLRESERAVEALVNHETQRGVKPENIVLAGFSQGGALALFAGLRYPEKLAGIMALSCYLPAPQTLAEEA
HSANFGIPIFMAHGVGDNVIPITLAAASRQQLLGTGYPVEWREYGMAHTVCREEIHDIRNWLQRVLA

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI9966764, Length=224, Percent_Identity=33.0357142857143, Blast_Score=135, Evalue=2e-32,
Organism=Homo sapiens, GI5453722, Length=222, Percent_Identity=31.981981981982, Blast_Score=127, Evalue=6e-30,
Organism=Homo sapiens, GI20270341, Length=227, Percent_Identity=21.1453744493392, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17508057, Length=219, Percent_Identity=34.703196347032, Blast_Score=129, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6323147, Length=212, Percent_Identity=34.9056603773585, Blast_Score=120, Evalue=1e-28,
Organism=Drosophila melanogaster, GI45553055, Length=214, Percent_Identity=34.1121495327103, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI45553057, Length=214, Percent_Identity=34.1121495327103, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI21357257, Length=214, Percent_Identity=34.1121495327103, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI161078179, Length=220, Percent_Identity=26.8181818181818, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 24835; Mature: 24835

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISGSPDLLPSIELETAAHPTRTIIWMHGLGADGSDFVPVVDELALPSIPAVRFVFPHAP
CCCCCCCCCCCCEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHCCCCCCCEEEEECCCC
TRPVSINRGMVMRAWYDYDIVDGAKLQENMATLRESERAVEALVNHETQRGVKPENIVLA
CCCEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
GFSQGGALALFAGLRYPEKLAGIMALSCYLPAPQTLAEEAHSANFGIPIFMAHGVGDNVI
ECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEECCCCCCEE
PITLAAASRQQLLGTGYPVEWREYGMAHTVCREEIHDIRNWLQRVLA
EEEEECHHHHHHHCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MISGSPDLLPSIELETAAHPTRTIIWMHGLGADGSDFVPVVDELALPSIPAVRFVFPHAP
CCCCCCCCCCCCEECCCCCCCEEEEEEECCCCCCCCCHHHHHHHCCCCCCCEEEEECCCC
TRPVSINRGMVMRAWYDYDIVDGAKLQENMATLRESERAVEALVNHETQRGVKPENIVLA
CCCEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEE
GFSQGGALALFAGLRYPEKLAGIMALSCYLPAPQTLAEEAHSANFGIPIFMAHGVGDNVI
ECCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEECCCCCCEE
PITLAAASRQQLLGTGYPVEWREYGMAHTVCREEIHDIRNWLQRVLA
EEEEECHHHHHHHCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA