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The map label for this gene is mltF

Identifier: 82703428

GI number: 82703428

Start: 2628831

End: 2630267

Strand: Direct

Name: mltF

Synonym: Nmul_A2311

Alternate gene names: 82703428

Gene position: 2628831-2630267 (Clockwise)

Preceding gene: 82703426

Following gene: 82703429

Centisome position: 82.56

GC content: 51.7

Gene sequence:

>1437_bases
ATGTTTCCCGATTCTAGTTATCTCTTCTCAATGCGATCTCTTTCTCGTTTTTTAATTGCTATTTTCGGTTGCGGCGCTCT
GCTCGCTTCCTGTGATTCGTTTGAAAGATCGGTATTGCCGTTCGACAAAACGGATGAGCTGGTGGTGATCACCGTCAATA
GCCCGGATACGTATTACGAGAATGCAGAAGGCAGTTATGCCGGGCTGGATTACGACCTCGCCACTGAGTTTGCGAAAGAG
CTGGGAATGAAAGTGAGATTCAAGACAGTGCCCAGACTGGATAAGGCATGGTCTCTCCTTGAAAAGCATAAAGGACACTT
TGCCGCCGGCATGAATATCAGCGCAAAGCACTCGCGGCATGTCGCGTTCGGTCCGATTTACCAGCTCGTACAACCCCAGC
TCGCCTATAACACGGACTACAACAAGCCGAAAAACCTTCATCAGCTCGGTGGAAGAACCATTCGCATTGCGAAAGGCGTA
ACCCATGCTGAACAGCTGAACAAGGCAAAGCACGAGGTGCCCGAGCTGAAATGGAAAGAGATGGACCTCACGCCCGACGA
ACTGCTGGCGAGACTGGCGGAAGGCAAGGTCGATTATGTGGTAGCGGATTCAACCCAGATCAATCTCGCGAAGAATTTTT
ATCCTAATCTGAATGCAGCATTCAATTTGGGCGATTCGGTAGGACGAGCATGGGCATTTTCACCCTTTGCCGAACAGGCG
TTACTGGAGGCGACACAAAAATTCTTCACGCGCATCCAGCAGGATGGGACGTTGACGCGCCTGCTCGACCGATACTACGG
ACACATAGAACGTCTTCATCATACGGATGTAAACGGCATTCTTGCGAAACGCCGCACCATTTTGCCTGAATTGCGCGAAC
ATTTTTATGAAGCCGAAGAGTTGAGCGGGATCGACTGGAGATTGATTGCGGCACTCGCCTATCAGGAATCGCACTGGGAC
GCCCTGGCGACTTCTCCCTCCAACGTGCGCGGCATCATGATGTTGACGGAAATCACGGCAGACCGGATGAAAGTGACGGA
TAGACTGGATGCACGGCAAAGCATACTTGCGGGAGCGCGCTACTTCGCATTACTCAAGGATAAACTGCCCACACGCATCA
AGGAGCCGGATCGCACCTGGATGGCACTGGCTGCCTATAATCAGGGGCCGAGTCACCTGGAAGATGCCCGGATACTGGCG
CAAAAGATGGGGTTGAGCCCTGATGCTTGGGTCGACCTGAAAAAAACGCTGCCGTTGCTGAGCCAAAGCGAACACTTCCG
TACTCTCAGGCATGGTTTTGCGCGCGGAGGTCAAGCAGTTGTACTGGCGGAATCAGTACGGATCTATTATGAGATTTTGC
AGAAATACGAACCTCCATATTCTTGGGGTTTTCCCATCGTTGCCAGAAAGGAGGATGATTCCTGGCAGGAGTTCTGA

Upstream 100 bases:

>100_bases
GTCAGCGCCGTTATGGCCACTTCGGTACCTCTCCGTTGATGAACTATTTTATCAGCTTATCCTATGTTTTTCTCGCGCTT
GGACAAGACTCGATGGCAGA

Downstream 100 bases:

>100_bases
TGCCTTCTTTCACGGGCAACAATCCGTCTCAAGGCAATAATGGACAGCAAACAATAGTTGTTGAGCGATTGCAGCAGGCA
GGCTATATTGAACCGGTTTC

Product: putative transglycosylase

Products: NA

Alternate protein names: Murein lyase F

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MFPDSSYLFSMRSLSRFLIAIFGCGALLASCDSFERSVLPFDKTDELVVITVNSPDTYYENAEGSYAGLDYDLATEFAKE
LGMKVRFKTVPRLDKAWSLLEKHKGHFAAGMNISAKHSRHVAFGPIYQLVQPQLAYNTDYNKPKNLHQLGGRTIRIAKGV
THAEQLNKAKHEVPELKWKEMDLTPDELLARLAEGKVDYVVADSTQINLAKNFYPNLNAAFNLGDSVGRAWAFSPFAEQA
LLEATQKFFTRIQQDGTLTRLLDRYYGHIERLHHTDVNGILAKRRTILPELREHFYEAEELSGIDWRLIAALAYQESHWD
ALATSPSNVRGIMMLTEITADRMKVTDRLDARQSILAGARYFALLKDKLPTRIKEPDRTWMALAAYNQGPSHLEDARILA
QKMGLSPDAWVDLKKTLPLLSQSEHFRTLRHGFARGGQAVVLAESVRIYYEILQKYEPPYSWGFPIVARKEDDSWQEF

Sequences:

>Translated_478_residues
MFPDSSYLFSMRSLSRFLIAIFGCGALLASCDSFERSVLPFDKTDELVVITVNSPDTYYENAEGSYAGLDYDLATEFAKE
LGMKVRFKTVPRLDKAWSLLEKHKGHFAAGMNISAKHSRHVAFGPIYQLVQPQLAYNTDYNKPKNLHQLGGRTIRIAKGV
THAEQLNKAKHEVPELKWKEMDLTPDELLARLAEGKVDYVVADSTQINLAKNFYPNLNAAFNLGDSVGRAWAFSPFAEQA
LLEATQKFFTRIQQDGTLTRLLDRYYGHIERLHHTDVNGILAKRRTILPELREHFYEAEELSGIDWRLIAALAYQESHWD
ALATSPSNVRGIMMLTEITADRMKVTDRLDARQSILAGARYFALLKDKLPTRIKEPDRTWMALAAYNQGPSHLEDARILA
QKMGLSPDAWVDLKKTLPLLSQSEHFRTLRHGFARGGQAVVLAESVRIYYEILQKYEPPYSWGFPIVARKEDDSWQEF
>Mature_478_residues
MFPDSSYLFSMRSLSRFLIAIFGCGALLASCDSFERSVLPFDKTDELVVITVNSPDTYYENAEGSYAGLDYDLATEFAKE
LGMKVRFKTVPRLDKAWSLLEKHKGHFAAGMNISAKHSRHVAFGPIYQLVQPQLAYNTDYNKPKNLHQLGGRTIRIAKGV
THAEQLNKAKHEVPELKWKEMDLTPDELLARLAEGKVDYVVADSTQINLAKNFYPNLNAAFNLGDSVGRAWAFSPFAEQA
LLEATQKFFTRIQQDGTLTRLLDRYYGHIERLHHTDVNGILAKRRTILPELREHFYEAEELSGIDWRLIAALAYQESHWD
ALATSPSNVRGIMMLTEITADRMKVTDRLDARQSILAGARYFALLKDKLPTRIKEPDRTWMALAAYNQGPSHLEDARILA
QKMGLSPDAWVDLKKTLPLLSQSEHFRTLRHGFARGGQAVVLAESVRIYYEILQKYEPPYSWGFPIVARKEDDSWQEF

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family

Homologues:

Organism=Escherichia coli, GI171474010, Length=409, Percent_Identity=39.8533007334963, Blast_Score=280, Evalue=1e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MLTF_NITMU (Q2Y6L9)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_412994.1
- ProteinModelPortal:   Q2Y6L9
- STRING:   Q2Y6L9
- GeneID:   3786717
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2311
- eggNOG:   COG4623
- HOGENOM:   HBG644469
- OMA:   QYVENIR
- PhylomeDB:   Q2Y6L9
- ProtClustDB:   PRK10859
- BioCyc:   NMUL323848:NMUL_A2311-MONOMER
- HAMAP:   MF_02016
- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189
- SMART:   SM00062

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT

EC number: NA

Molecular weight: Translated: 54438; Mature: 54438

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: ACT_SITE 316-316

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPDSSYLFSMRSLSRFLIAIFGCGALLASCDSFERSVLPFDKTDELVVITVNSPDTYYE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCC
NAEGSYAGLDYDLATEFAKELGMKVRFKTVPRLDKAWSLLEKHKGHFAAGMNISAKHSRH
CCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCEEECCCCCCCCCCC
VAFGPIYQLVQPQLAYNTDYNKPKNLHQLGGRTIRIAKGVTHAEQLNKAKHEVPELKWKE
CCHHHHHHHHCCHHHCCCCCCCCCHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCCCC
MDLTPDELLARLAEGKVDYVVADSTQINLAKNFYPNLNAAFNLGDSVGRAWAFSPFAEQA
CCCCHHHHHHHHHCCCEEEEEECCCEEEEHHHCCCCCCCEECCCHHHCCCCCCCHHHHHH
LLEATQKFFTRIQQDGTLTRLLDRYYGHIERLHHTDVNGILAKRRTILPELREHFYEAEE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LSGIDWRLIAALAYQESHWDALATSPSNVRGIMMLTEITADRMKVTDRLDARQSILAGAR
HCCCCHHHHHHHHHHHHCCHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
YFALLKDKLPTRIKEPDRTWMALAAYNQGPSHLEDARILAQKMGLSPDAWVDLKKTLPLL
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCHHEEHHHHHHHH
SQSEHFRTLRHGFARGGQAVVLAESVRIYYEILQKYEPPYSWGFPIVARKEDDSWQEF
CCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCC
>Mature Secondary Structure
MFPDSSYLFSMRSLSRFLIAIFGCGALLASCDSFERSVLPFDKTDELVVITVNSPDTYYE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCC
NAEGSYAGLDYDLATEFAKELGMKVRFKTVPRLDKAWSLLEKHKGHFAAGMNISAKHSRH
CCCCCCCCCCHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCEEECCCCCCCCCCC
VAFGPIYQLVQPQLAYNTDYNKPKNLHQLGGRTIRIAKGVTHAEQLNKAKHEVPELKWKE
CCHHHHHHHHCCHHHCCCCCCCCCHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCCCC
MDLTPDELLARLAEGKVDYVVADSTQINLAKNFYPNLNAAFNLGDSVGRAWAFSPFAEQA
CCCCHHHHHHHHHCCCEEEEEECCCEEEEHHHCCCCCCCEECCCHHHCCCCCCCHHHHHH
LLEATQKFFTRIQQDGTLTRLLDRYYGHIERLHHTDVNGILAKRRTILPELREHFYEAEE
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
LSGIDWRLIAALAYQESHWDALATSPSNVRGIMMLTEITADRMKVTDRLDARQSILAGAR
HCCCCHHHHHHHHHHHHCCHHHCCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHH
YFALLKDKLPTRIKEPDRTWMALAAYNQGPSHLEDARILAQKMGLSPDAWVDLKKTLPLL
HHHHHHHHCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCHHEEHHHHHHHH
SQSEHFRTLRHGFARGGQAVVLAESVRIYYEILQKYEPPYSWGFPIVARKEDDSWQEF
CCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA