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The map label for this gene is lysS [H]

Identifier: 82703393

GI number: 82703393

Start: 2586860

End: 2588383

Strand: Direct

Name: lysS [H]

Synonym: Nmul_A2275

Alternate gene names: 82703393

Gene position: 2586860-2588383 (Clockwise)

Preceding gene: 82703392

Following gene: 82703394

Centisome position: 81.24

GC content: 56.04

Gene sequence:

>1524_bases
ATGGATGAGGCTTTAGAGAAAGATTCGGAGGAAACGAATCGGTTTCAGGCAGAGATCAGGCTCGTCGAGGAGCGGCGAGC
AAAACTGGATGCGCTGCGTGCAACGGGCAATGCCTTTCCCAATGACTTTCCCCGCGAACATTTTTCGGCGGACCTTCATA
ACACGTATAGCGAACACTCCACCGAAACACTGGAGGCAGAACCGGTACCCGTGCGCGTGGCTGGCCGGATGGTCCTCAAG
CGGGTCATGGGCAAGGCAAGCTTTGCCACCCTGCAGGATATGCGCGGTCGCATCCAGCTTTACATTGCAAAGGATGTAGC
GGGCGACAGCGCTTATGGCGCATTCAGGCATTATGATCTTGGGGACATATTGGGTGCGGAGGGTACACTGTTTCGGACCC
GCACCGGGGAGTTGTCGGTACGGGTGACAAACCTGAGATTGCTCACGAAATCGCTGCGCCCACTGCCGGAAAAATTTCAT
GGTCTGAGCGACCAGGAGCAGAAGTACCGCCAGCGCTATCTCGACCTCATCACGAATGAAAGCAGTCGCAAGGTGTTTAT
CGCGCGCTCCAGGATCATCCAGGCCATCCGCGAATTTCTGGTCGGGCATGGCTATCTGGAAGTCGAAACGCCCATGATGC
ATCCTATCCCGGGGGGGGCGGCTGCACGCCCTTTCATCACGCATCACAACGCGCTGGACATGCAGTTGTATCTGCGTATC
GCACCCGAGCTTTATCTCAAGCGTCTGGTAATAGGCGGATTGGAAAAAGTATTCGAAATAAACCGCAGCTTCAGGAATGA
GGGGATTTCCACTCGGCATAACCCCGAATTCACGATGCTGGAGTTTTACGAGGCCTACCGGGACTATGCGTATCTGATGG
ATCTTACCGAAGCTCTGTTGCGGGAAGTAACGCTAAAGGTGCTGGGCGCTGCCAGCTTCACGTATCAGGAGCGCGAGCTG
GATCTGGCCCAGCCCTTCGCGCGCCTGACCATCACCGAGGCTATCCGGAAATTTCATCCCGAATATACGGAGGCGCAGCT
CGACGATCGTGCATTCCTGATCGACAAGTTGCAGGAACTCGGAATTCCTTATAAACCCGGGGACGGCGTAGGCGGATTAC
AGCTTACCCTGTTTGATGAAACTACCGAGCATCTGCTGTTCGACCCTACCTTCATTGTCGATTATCCGGCGGAAGTCTCG
CCGCTTGCGCGTCGCAACGACAGGAATCCGGCAATAACCGACCGGTTTGAGCTCTATATAGCCGGGCGTGAGATTGCAAA
CGGATTCTCAGAGCTGAATGATCCGGAGGATCAGGCGGTGCGCTTCCTGGAGCAGGCAAGAGCCAAGGAAGCAGGCGATG
CGGAAGCCATGCACTACGATGCCGATTATATCCGCGCCCTCGAATATGGCCTGCCTCCCACCGCCGGGGAAGGCATCGGC
ATCGATCGGCTGGTCATGCTCCTGACCAATAGCCCGAGTATCCGCGATGTCATTCTGTTCCCCCAATTGCGGCGGGAGGA
TTGA

Upstream 100 bases:

>100_bases
GGGGGTGGAAAGCGGCAACACCCAGGCTATCCTCGACGGGGATCTGGATGATTTTATCGAAGCAAGCTTGAAACAAGGCG
TATAAAAGGACCGATCGGAG

Downstream 100 bases:

>100_bases
ATCTCGATCGTTTTCGGCTGTCCAGGGCCTGTTTTTTGAAAGGCTGCGTAACAAATACACTCTTCAATCTACTCTTCAGG
CAGCTGAACTGCTCCTGACC

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MDEALEKDSEETNRFQAEIRLVEERRAKLDALRATGNAFPNDFPREHFSADLHNTYSEHSTETLEAEPVPVRVAGRMVLK
RVMGKASFATLQDMRGRIQLYIAKDVAGDSAYGAFRHYDLGDILGAEGTLFRTRTGELSVRVTNLRLLTKSLRPLPEKFH
GLSDQEQKYRQRYLDLITNESSRKVFIARSRIIQAIREFLVGHGYLEVETPMMHPIPGGAAARPFITHHNALDMQLYLRI
APELYLKRLVIGGLEKVFEINRSFRNEGISTRHNPEFTMLEFYEAYRDYAYLMDLTEALLREVTLKVLGAASFTYQEREL
DLAQPFARLTITEAIRKFHPEYTEAQLDDRAFLIDKLQELGIPYKPGDGVGGLQLTLFDETTEHLLFDPTFIVDYPAEVS
PLARRNDRNPAITDRFELYIAGREIANGFSELNDPEDQAVRFLEQARAKEAGDAEAMHYDADYIRALEYGLPPTAGEGIG
IDRLVMLLTNSPSIRDVILFPQLRRED

Sequences:

>Translated_507_residues
MDEALEKDSEETNRFQAEIRLVEERRAKLDALRATGNAFPNDFPREHFSADLHNTYSEHSTETLEAEPVPVRVAGRMVLK
RVMGKASFATLQDMRGRIQLYIAKDVAGDSAYGAFRHYDLGDILGAEGTLFRTRTGELSVRVTNLRLLTKSLRPLPEKFH
GLSDQEQKYRQRYLDLITNESSRKVFIARSRIIQAIREFLVGHGYLEVETPMMHPIPGGAAARPFITHHNALDMQLYLRI
APELYLKRLVIGGLEKVFEINRSFRNEGISTRHNPEFTMLEFYEAYRDYAYLMDLTEALLREVTLKVLGAASFTYQEREL
DLAQPFARLTITEAIRKFHPEYTEAQLDDRAFLIDKLQELGIPYKPGDGVGGLQLTLFDETTEHLLFDPTFIVDYPAEVS
PLARRNDRNPAITDRFELYIAGREIANGFSELNDPEDQAVRFLEQARAKEAGDAEAMHYDADYIRALEYGLPPTAGEGIG
IDRLVMLLTNSPSIRDVILFPQLRRED
>Mature_507_residues
MDEALEKDSEETNRFQAEIRLVEERRAKLDALRATGNAFPNDFPREHFSADLHNTYSEHSTETLEAEPVPVRVAGRMVLK
RVMGKASFATLQDMRGRIQLYIAKDVAGDSAYGAFRHYDLGDILGAEGTLFRTRTGELSVRVTNLRLLTKSLRPLPEKFH
GLSDQEQKYRQRYLDLITNESSRKVFIARSRIIQAIREFLVGHGYLEVETPMMHPIPGGAAARPFITHHNALDMQLYLRI
APELYLKRLVIGGLEKVFEINRSFRNEGISTRHNPEFTMLEFYEAYRDYAYLMDLTEALLREVTLKVLGAASFTYQEREL
DLAQPFARLTITEAIRKFHPEYTEAQLDDRAFLIDKLQELGIPYKPGDGVGGLQLTLFDETTEHLLFDPTFIVDYPAEVS
PLARRNDRNPAITDRFELYIAGREIANGFSELNDPEDQAVRFLEQARAKEAGDAEAMHYDADYIRALEYGLPPTAGEGIG
IDRLVMLLTNSPSIRDVILFPQLRRED

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=507, Percent_Identity=42.0118343195266, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI194272210, Length=507, Percent_Identity=42.0118343195266, Blast_Score=365, Evalue=1e-101,
Organism=Homo sapiens, GI45439306, Length=346, Percent_Identity=26.878612716763, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI40789249, Length=298, Percent_Identity=23.8255033557047, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1790571, Length=505, Percent_Identity=60.3960396039604, Blast_Score=616, Evalue=1e-177,
Organism=Escherichia coli, GI1789256, Length=484, Percent_Identity=61.5702479338843, Blast_Score=606, Evalue=1e-174,
Organism=Escherichia coli, GI87082379, Length=328, Percent_Identity=32.9268292682927, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1788173, Length=277, Percent_Identity=25.6317689530686, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=515, Percent_Identity=40.5825242718447, Blast_Score=345, Evalue=3e-95,
Organism=Caenorhabditis elegans, GI17535927, Length=515, Percent_Identity=40.5825242718447, Blast_Score=345, Evalue=5e-95,
Organism=Caenorhabditis elegans, GI71994340, Length=505, Percent_Identity=40.990099009901, Blast_Score=342, Evalue=3e-94,
Organism=Caenorhabditis elegans, GI32566633, Length=275, Percent_Identity=22.9090909090909, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17551876, Length=341, Percent_Identity=24.633431085044, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=512, Percent_Identity=42.578125, Blast_Score=359, Evalue=1e-100,
Organism=Saccharomyces cerevisiae, GI6324256, Length=431, Percent_Identity=33.8747099767981, Blast_Score=186, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6323011, Length=351, Percent_Identity=26.7806267806268, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24640849, Length=496, Percent_Identity=40.7258064516129, Blast_Score=351, Evalue=7e-97,
Organism=Drosophila melanogaster, GI24640851, Length=496, Percent_Identity=40.7258064516129, Blast_Score=351, Evalue=8e-97,
Organism=Drosophila melanogaster, GI24584738, Length=358, Percent_Identity=25.4189944134078, Blast_Score=86, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 57909; Mature: 57909

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDEALEKDSEETNRFQAEIRLVEERRAKLDALRATGNAFPNDFPREHFSADLHNTYSEHS
CCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHCC
TETLEAEPVPVRVAGRMVLKRVMGKASFATLQDMRGRIQLYIAKDVAGDSAYGAFRHYDL
CCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHCCCH
GDILGAEGTLFRTRTGELSVRVTNLRLLTKSLRPLPEKFHGLSDQEQKYRQRYLDLITNE
HHHCCCCCCEEEEECCCEEEEEEHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHCCC
SSRKVFIARSRIIQAIREFLVGHGYLEVETPMMHPIPGGAAARPFITHHNALDMQLYLRI
CCCEEEHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCEEECCCCCCHHHHHHH
APELYLKRLVIGGLEKVFEINRSFRNEGISTRHNPEFTMLEFYEAYRDYAYLMDLTEALL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
REVTLKVLGAASFTYQERELDLAQPFARLTITEAIRKFHPEYTEAQLDDRAFLIDKLQEL
HHHHHHHHHHHHHEEHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHC
GIPYKPGDGVGGLQLTLFDETTEHLLFDPTFIVDYPAEVSPLARRNDRNPAITDRFELYI
CCCCCCCCCCCCEEEEEECCCCHHEEECCEEEEECCCCCCHHHHCCCCCCCCCCEEEEEE
AGREIANGFSELNDPEDQAVRFLEQARAKEAGDAEAMHYDADYIRALEYGLPPTAGEGIG
ECHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHEECCHHHHHHHHHCCCCCCCCCCC
IDRLVMLLTNSPSIRDVILFPQLRRED
HHHHHHEEECCCCCEEEEECCCCCCCC
>Mature Secondary Structure
MDEALEKDSEETNRFQAEIRLVEERRAKLDALRATGNAFPNDFPREHFSADLHNTYSEHS
CCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHCC
TETLEAEPVPVRVAGRMVLKRVMGKASFATLQDMRGRIQLYIAKDVAGDSAYGAFRHYDL
CCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEEEEEECCCCCCCCCHHCCCH
GDILGAEGTLFRTRTGELSVRVTNLRLLTKSLRPLPEKFHGLSDQEQKYRQRYLDLITNE
HHHCCCCCCEEEEECCCEEEEEEHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHHHHHCCC
SSRKVFIARSRIIQAIREFLVGHGYLEVETPMMHPIPGGAAARPFITHHNALDMQLYLRI
CCCEEEHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCEEECCCCCCHHHHHHH
APELYLKRLVIGGLEKVFEINRSFRNEGISTRHNPEFTMLEFYEAYRDYAYLMDLTEALL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
REVTLKVLGAASFTYQERELDLAQPFARLTITEAIRKFHPEYTEAQLDDRAFLIDKLQEL
HHHHHHHHHHHHHEEHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHC
GIPYKPGDGVGGLQLTLFDETTEHLLFDPTFIVDYPAEVSPLARRNDRNPAITDRFELYI
CCCCCCCCCCCCEEEEEECCCCHHEEECCEEEEECCCCCCHHHHCCCCCCCCCCEEEEEE
AGREIANGFSELNDPEDQAVRFLEQARAKEAGDAEAMHYDADYIRALEYGLPPTAGEGIG
ECHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHEECCHHHHHHHHHCCCCCCCCCCC
IDRLVMLLTNSPSIRDVILFPQLRRED
HHHHHHEEECCCCCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]