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The map label for this gene is lacZ [H]

Identifier: 82703061

GI number: 82703061

Start: 2231010

End: 2232791

Strand: Reverse

Name: lacZ [H]

Synonym: Nmul_A1940

Alternate gene names: 82703061

Gene position: 2232791-2231010 (Counterclockwise)

Preceding gene: 82703062

Following gene: 82703060

Centisome position: 70.12

GC content: 56.62

Gene sequence:

>1782_bases
TTGAAGTTCGCGCACCCTCGACCGCAGATGCAGCGCTCCAATTGGACCTCGCTCAACGGAGTCTGGCGCTTTCGCTACGA
CGAGGCGCGTACTTTTACTCACCCCTCTCAGATAGAATCCTGGCCGATGGAAATCATCGTGCCGTTTCCACCCGAATCCG
AGGCAAGCGGAATCGGTGACCGCAGTTTTCATTCACTCTGCTGGTATGAGCGCGATTTTGATTTCGAGCCCTCCTCGGAG
CGGGTAATCCTGCATTTCGGTGCCGTGGACTACTCCGCAAAGGTATGGGTCAACGGTCGTTTTGCCGCCAGCCACGAAGG
GGGGCATACTCCATTCTGGGGGGACATCACCCCTCTGCTCGATCCATCCGGAAAACAGAAGGTGACGGTACAGGTGGAGG
ACGATCCGCACGAGCTGGCCAAACCGCGTGGAAAGCAGGATTGGCAGCTCGAGCCTCATGCCATCTGGTATCCCCGTACC
ACTGGCATCTGGCAGATGGTCTGGATCGAGCGGGTATCCGAAAATTACATTGAAAAGATTCGGTGGACGCCCCAGGTCGA
GATATATGCGATAGGCTTCGAAGCGCGCGTAATAGGAGAAGAGGCCGACGAACTGGCAGTAGACGTATGCTTGCGCCACG
GGGAGCAGTTGCTCGCGCATGACCGCTATCGGGTAGTCGAACGGGAAGTAGACCGCGTCATCATATTGTCTGACCCTGGA
ATTGATGATTTTCGTAATGAGTTGCTATGGAGCCCGGAACGTCCCACCTTGATCGATGCTGTTGTACGACTGATGCGGGG
CGAGGAGGTGGTCGACGAGTTCATCTCCTACACTGCAATGCGTTCCGTCAACATCCTGCGCGACCGCTTCATGCTGAACG
GTCGTCCCTACACGCTGAGGCTCGTGCTTGACCAGGGCTACTGGCCGGAGACGCTGCTGGCTGCGCCAAGCGACGACGCC
CTGCGAGGCGATGTGGAACTTGCAAAGGCAATGGGCTTCAACGGGGTGCGCAAGCATCAAAAGATAGAGGACCCGCGCTA
TCTTTATTGGGCGGACAGGCTCGGGCTGATGGTATGGGAAGAAATGCCTTCCGCATATCGCTTCACCCGCAGCGCCATCA
AGAGGATGGTGCGGGAATGGACGGAGGCCATCGAGCGGGATTACAGCCATCCCTGCGTCATTGTATGGGTACCTTTCAAT
GAATCCTGGGGAGTACCGGAACTTACCGCGGTCCGTAAACAGCGGCACGCTGTCGAGGCACTATATCACTTGACCAAAAC
CCTGGATGCGACGCGCCCGGTAATCGGCAACGATGGATGGGAAAGCAGTGCTACGGATATCATCGGTATTCACGATTATG
ACGCAAACATTGAACATCTGCGCCAGCGTTATGGCGCCGAAATAAAACCTGAACAGTTGTTCGACCGTCGGCGCCCGGGG
GGGCGGATTCTCACCCTTGATGGCTACCCGCATCGAGGCCAGCCGATCATGTTAAGCGAATTCGGGGGGATTGCTTTCGC
CAAGTGCCCGCAACCCGGCGTCGAGCATACTTGGGGCTATACTGTTGCCCACGCCGAAGAGGAATTTGCGCGTATGTATG
CCGAGTTGATGCATACAGTGATTCATACGGCTCTCTTCAGCGGCTTTTGCTATACCCAGTTTGCCGATACCTTTCAGGAA
GCGAACGGACTGCTGTGCGCGGATCGTACTCCCAAGATTCCCATTGAGCAAATCGCCCGCGTCACGCGCATCTCGCCCAC
CTATATACCCGGGGGTGTTTAG

Upstream 100 bases:

>100_bases
CTGTAGTCCTCTATCCCATCAGATATCAGATCAGTCATACCTGAGTAGCCGCTCGTCTCCAATGACGAGCAAGCATGCAG
CGCTTTAGGAGGAGAATGCA

Downstream 100 bases:

>100_bases
GTGTATTCGCTTGAGCTCACCAAGCCGGATGGCCGTCGGCTGATTCTGTATTCCCGGGAGGCTATTAATCCTGATATCGT
TGCGCCCAGCCCTTTCACGG

Product: glycoside hydrolase family protein

Products: NA

Alternate protein names: Beta-gal; Lactase [H]

Number of amino acids: Translated: 593; Mature: 593

Protein sequence:

>593_residues
MKFAHPRPQMQRSNWTSLNGVWRFRYDEARTFTHPSQIESWPMEIIVPFPPESEASGIGDRSFHSLCWYERDFDFEPSSE
RVILHFGAVDYSAKVWVNGRFAASHEGGHTPFWGDITPLLDPSGKQKVTVQVEDDPHELAKPRGKQDWQLEPHAIWYPRT
TGIWQMVWIERVSENYIEKIRWTPQVEIYAIGFEARVIGEEADELAVDVCLRHGEQLLAHDRYRVVEREVDRVIILSDPG
IDDFRNELLWSPERPTLIDAVVRLMRGEEVVDEFISYTAMRSVNILRDRFMLNGRPYTLRLVLDQGYWPETLLAAPSDDA
LRGDVELAKAMGFNGVRKHQKIEDPRYLYWADRLGLMVWEEMPSAYRFTRSAIKRMVREWTEAIERDYSHPCVIVWVPFN
ESWGVPELTAVRKQRHAVEALYHLTKTLDATRPVIGNDGWESSATDIIGIHDYDANIEHLRQRYGAEIKPEQLFDRRRPG
GRILTLDGYPHRGQPIMLSEFGGIAFAKCPQPGVEHTWGYTVAHAEEEFARMYAELMHTVIHTALFSGFCYTQFADTFQE
ANGLLCADRTPKIPIEQIARVTRISPTYIPGGV

Sequences:

>Translated_593_residues
MKFAHPRPQMQRSNWTSLNGVWRFRYDEARTFTHPSQIESWPMEIIVPFPPESEASGIGDRSFHSLCWYERDFDFEPSSE
RVILHFGAVDYSAKVWVNGRFAASHEGGHTPFWGDITPLLDPSGKQKVTVQVEDDPHELAKPRGKQDWQLEPHAIWYPRT
TGIWQMVWIERVSENYIEKIRWTPQVEIYAIGFEARVIGEEADELAVDVCLRHGEQLLAHDRYRVVEREVDRVIILSDPG
IDDFRNELLWSPERPTLIDAVVRLMRGEEVVDEFISYTAMRSVNILRDRFMLNGRPYTLRLVLDQGYWPETLLAAPSDDA
LRGDVELAKAMGFNGVRKHQKIEDPRYLYWADRLGLMVWEEMPSAYRFTRSAIKRMVREWTEAIERDYSHPCVIVWVPFN
ESWGVPELTAVRKQRHAVEALYHLTKTLDATRPVIGNDGWESSATDIIGIHDYDANIEHLRQRYGAEIKPEQLFDRRRPG
GRILTLDGYPHRGQPIMLSEFGGIAFAKCPQPGVEHTWGYTVAHAEEEFARMYAELMHTVIHTALFSGFCYTQFADTFQE
ANGLLCADRTPKIPIEQIARVTRISPTYIPGGV
>Mature_593_residues
MKFAHPRPQMQRSNWTSLNGVWRFRYDEARTFTHPSQIESWPMEIIVPFPPESEASGIGDRSFHSLCWYERDFDFEPSSE
RVILHFGAVDYSAKVWVNGRFAASHEGGHTPFWGDITPLLDPSGKQKVTVQVEDDPHELAKPRGKQDWQLEPHAIWYPRT
TGIWQMVWIERVSENYIEKIRWTPQVEIYAIGFEARVIGEEADELAVDVCLRHGEQLLAHDRYRVVEREVDRVIILSDPG
IDDFRNELLWSPERPTLIDAVVRLMRGEEVVDEFISYTAMRSVNILRDRFMLNGRPYTLRLVLDQGYWPETLLAAPSDDA
LRGDVELAKAMGFNGVRKHQKIEDPRYLYWADRLGLMVWEEMPSAYRFTRSAIKRMVREWTEAIERDYSHPCVIVWVPFN
ESWGVPELTAVRKQRHAVEALYHLTKTLDATRPVIGNDGWESSATDIIGIHDYDANIEHLRQRYGAEIKPEQLFDRRRPG
GRILTLDGYPHRGQPIMLSEFGGIAFAKCPQPGVEHTWGYTVAHAEEEFARMYAELMHTVIHTALFSGFCYTQFADTFQE
ANGLLCADRTPKIPIEQIARVTRISPTYIPGGV

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=497, Percent_Identity=22.7364185110664, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1787903, Length=467, Percent_Identity=22.6980728051392, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1786539, Length=484, Percent_Identity=25.6198347107438, Blast_Score=74, Evalue=3e-14,
Organism=Drosophila melanogaster, GI19922582, Length=487, Percent_Identity=23.4086242299795, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24655438, Length=487, Percent_Identity=23.4086242299795, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI62471735, Length=487, Percent_Identity=23.4086242299795, Blast_Score=105, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003344
- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.23 [H]

Molecular weight: Translated: 68584; Mature: 68584

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFAHPRPQMQRSNWTSLNGVWRFRYDEARTFTHPSQIESWPMEIIVPFPPESEASGIGD
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHCCCCEEEEEECCCCCCCCCCCC
RSFHSLCWYERDFDFEPSSERVILHFGAVDYSAKVWVNGRFAASHEGGHTPFWGDITPLL
CCHHHHHEECCCCCCCCCCCEEEEEEECCCCEEEEEEECEEEECCCCCCCCCCCCCCCCC
DPSGKQKVTVQVEDDPHELAKPRGKQDWQLEPHAIWYPRTTGIWQMVWIERVSENYIEKI
CCCCCEEEEEEECCCHHHHHCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHH
RWTPQVEIYAIGFEARVIGEEADELAVDVCLRHGEQLLAHDRYRVVEREVDRVIILSDPG
CCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEECCC
IDDFRNELLWSPERPTLIDAVVRLMRGEEVVDEFISYTAMRSVNILRDRFMLNGRPYTLR
CHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEE
LVLDQGYWPETLLAAPSDDALRGDVELAKAMGFNGVRKHQKIEDPRYLYWADRLGLMVWE
EEEECCCCCHHEEECCCCCCCCCHHHHHHHCCCCHHHHHHCCCCCCEEEEECCCCHHHHH
EMPSAYRFTRSAIKRMVREWTEAIERDYSHPCVIVWVPFNESWGVPELTAVRKQRHAVEA
HCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHH
LYHLTKTLDATRPVIGNDGWESSATDIIGIHDYDANIEHLRQRYGAEIKPEQLFDRRRPG
HHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHCCCCC
GRILTLDGYPHRGQPIMLSEFGGIAFAKCPQPGVEHTWGYTVAHAEEEFARMYAELMHTV
CEEEEECCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHH
IHTALFSGFCYTQFADTFQEANGLLCADRTPKIPIEQIARVTRISPTYIPGGV
HHHHHHHCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKFAHPRPQMQRSNWTSLNGVWRFRYDEARTFTHPSQIESWPMEIIVPFPPESEASGIGD
CCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHCCCCEEEEEECCCCCCCCCCCC
RSFHSLCWYERDFDFEPSSERVILHFGAVDYSAKVWVNGRFAASHEGGHTPFWGDITPLL
CCHHHHHEECCCCCCCCCCCEEEEEEECCCCEEEEEEECEEEECCCCCCCCCCCCCCCCC
DPSGKQKVTVQVEDDPHELAKPRGKQDWQLEPHAIWYPRTTGIWQMVWIERVSENYIEKI
CCCCCEEEEEEECCCHHHHHCCCCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHH
RWTPQVEIYAIGFEARVIGEEADELAVDVCLRHGEQLLAHDRYRVVEREVDRVIILSDPG
CCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEEEEECCC
IDDFRNELLWSPERPTLIDAVVRLMRGEEVVDEFISYTAMRSVNILRDRFMLNGRPYTLR
CHHHHHHHCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEE
LVLDQGYWPETLLAAPSDDALRGDVELAKAMGFNGVRKHQKIEDPRYLYWADRLGLMVWE
EEEECCCCCHHEEECCCCCCCCCHHHHHHHCCCCHHHHHHCCCCCCEEEEECCCCHHHHH
EMPSAYRFTRSAIKRMVREWTEAIERDYSHPCVIVWVPFNESWGVPELTAVRKQRHAVEA
HCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHH
LYHLTKTLDATRPVIGNDGWESSATDIIGIHDYDANIEHLRQRYGAEIKPEQLFDRRRPG
HHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHCCCCC
GRILTLDGYPHRGQPIMLSEFGGIAFAKCPQPGVEHTWGYTVAHAEEEFARMYAELMHTV
CEEEEECCCCCCCCEEEEECCCCEEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHH
IHTALFSGFCYTQFADTFQEANGLLCADRTPKIPIEQIARVTRISPTYIPGGV
HHHHHHHCCHHHHHHHHHHHCCCEEEECCCCCCCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA