Click here to switch to the map view.

The map label for this gene is folC [H]

Identifier: 82703030

GI number: 82703030

Start: 2199174

End: 2200502

Strand: Reverse

Name: folC [H]

Synonym: Nmul_A1909

Alternate gene names: 82703030

Gene position: 2200502-2199174 (Counterclockwise)

Preceding gene: 82703031

Following gene: 82703029

Centisome position: 69.11

GC content: 57.49

Gene sequence:

>1329_bases
ATGAGCACTTTCTCCTTTGCTTCCGCAACGCTGCCAGAGTGGCTGGACTACCTGGAACGCCTGCATCCCGCAGCTATCGA
AATGGGACTGGAGCGGATACGGCGCGTCCAGGCGGAACTGGAGCTCGAACCTTCATTTCCCATCATTTCCGTTGCGGGCA
CCAACGGGAAGGGATCGACATGCATGATGCTGGAAGCCATTCTGAGCCATGCCGGATATCGGGTCGGATGCTATACCTCC
CCTCATTTGCTGCGATATAACGAACGCGTGCGAATCGACCGGAAGGAGGCAAGCGATGATGAGCTGTGCGAGGCTTTCCG
CGCTGTCGAATCGGCGCGGATGAATAGCGCCGTATCCCTGACCTATTTCGAGTTTGGCACACTGGCCGCCATGTACTTGT
TCAGTCAGGCCGAAGTGGAAGTTGCAATTCTGGAGGTGGGATTGGGCGGGCGGCTGGATGCGGTCAATGTGTTCGAGGCC
GATTGCGCGGTCCTTACCAGCGTGGATTTCGATCATATGGATTATCTGGGCAATACGCGTGAACAGATCGGATTCGAAAA
GGCCGGAATTTTCAGGTCAGGGAAGGCCGCAATCTGTTCCGAACCGGACCTGCCCATAAGCGTGCGTCGTCATGCGGAAT
CGATCGGCGCCGACCTCATGCATATCGGAGAGCACTTCGGTTATTCAACCGCTCCACAGTCCTGGAGCTATTGGCGGAAC
GGCGAGAGCAGGCATGCACTCCCTTATCCGGCCTTGCGCGGCGCCTATCAGTTGAAAAATGCCAGTGCGTGCCTCGCCGC
CCTGGATTCCCTGAAAGATACATTGCCGGTTACCTTGAGCGACATTCGTCATGGTTTGCTGGAAGTGGTTTGGCCAGCCC
GGTTTCAGGTGTTGCCTGGACAGCCGGTCAGGGTGCTCGATGTTGCCCATAATCCAGGCGCGGCACGCGCATTGGCCGCC
AGCCTCGATAGCATGGGGCGTTATCCCAGAACGTACGCGGTATTTGCAATGCTCGGGGATAAAGATATCGCAGGCGTGGT
ACGGGAGTTGAGGTCCAGCGTGGATGTCTGGCTGGTATCGGGTATTGACGCTCCAAGAGGCGCTACGGCGGACGAAGCCG
CCACGCAGGTTGCCCAGGCGCTGCAAATCGCCGAGCCGCTCCCGGGGAATGCAGGAGAAGGCGCCGCCCATACCATCCGC
AAGTTCCGCAATCCATCCGAGGCATATGCTTACGCCTGTGAGCAGGCAGCCAGAAATGATAGAATTTGTGTTTTCGGCTC
ATTCCATACCGTGGCTGAAGTGTTAAGGAACAGAATTGAGCGCGGGTAG

Upstream 100 bases:

>100_bases
AAGATCGCCAATTTGTGCACGTTGCTGATGCACGCTTCCCCTCCCGCGCTCTGAGCATTTTCACTCGACGCATAATCATA
CTTACCGTGCCATCGCGGGC

Downstream 100 bases:

>100_bases
ATTTCCTGATATCTAATGGCAAAAACCATCACAGAAGAAGAGATTCAGCTAAGAAAGCGCGCCAGACGACGCCTGGTGGG
GGCAATCGTCCTGGCAACCG

Product: FolC bifunctional protein

Products: NA

Alternate protein names: Folylpolyglutamate synthase; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; Dihydrofolate synthase [H]

Number of amino acids: Translated: 442; Mature: 441

Protein sequence:

>442_residues
MSTFSFASATLPEWLDYLERLHPAAIEMGLERIRRVQAELELEPSFPIISVAGTNGKGSTCMMLEAILSHAGYRVGCYTS
PHLLRYNERVRIDRKEASDDELCEAFRAVESARMNSAVSLTYFEFGTLAAMYLFSQAEVEVAILEVGLGGRLDAVNVFEA
DCAVLTSVDFDHMDYLGNTREQIGFEKAGIFRSGKAAICSEPDLPISVRRHAESIGADLMHIGEHFGYSTAPQSWSYWRN
GESRHALPYPALRGAYQLKNASACLAALDSLKDTLPVTLSDIRHGLLEVVWPARFQVLPGQPVRVLDVAHNPGAARALAA
SLDSMGRYPRTYAVFAMLGDKDIAGVVRELRSSVDVWLVSGIDAPRGATADEAATQVAQALQIAEPLPGNAGEGAAHTIR
KFRNPSEAYAYACEQAARNDRICVFGSFHTVAEVLRNRIERG

Sequences:

>Translated_442_residues
MSTFSFASATLPEWLDYLERLHPAAIEMGLERIRRVQAELELEPSFPIISVAGTNGKGSTCMMLEAILSHAGYRVGCYTS
PHLLRYNERVRIDRKEASDDELCEAFRAVESARMNSAVSLTYFEFGTLAAMYLFSQAEVEVAILEVGLGGRLDAVNVFEA
DCAVLTSVDFDHMDYLGNTREQIGFEKAGIFRSGKAAICSEPDLPISVRRHAESIGADLMHIGEHFGYSTAPQSWSYWRN
GESRHALPYPALRGAYQLKNASACLAALDSLKDTLPVTLSDIRHGLLEVVWPARFQVLPGQPVRVLDVAHNPGAARALAA
SLDSMGRYPRTYAVFAMLGDKDIAGVVRELRSSVDVWLVSGIDAPRGATADEAATQVAQALQIAEPLPGNAGEGAAHTIR
KFRNPSEAYAYACEQAARNDRICVFGSFHTVAEVLRNRIERG
>Mature_441_residues
STFSFASATLPEWLDYLERLHPAAIEMGLERIRRVQAELELEPSFPIISVAGTNGKGSTCMMLEAILSHAGYRVGCYTSP
HLLRYNERVRIDRKEASDDELCEAFRAVESARMNSAVSLTYFEFGTLAAMYLFSQAEVEVAILEVGLGGRLDAVNVFEAD
CAVLTSVDFDHMDYLGNTREQIGFEKAGIFRSGKAAICSEPDLPISVRRHAESIGADLMHIGEHFGYSTAPQSWSYWRNG
ESRHALPYPALRGAYQLKNASACLAALDSLKDTLPVTLSDIRHGLLEVVWPARFQVLPGQPVRVLDVAHNPGAARALAAS
LDSMGRYPRTYAVFAMLGDKDIAGVVRELRSSVDVWLVSGIDAPRGATADEAATQVAQALQIAEPLPGNAGEGAAHTIRK
FRNPSEAYAYACEQAARNDRICVFGSFHTVAEVLRNRIERG

Specific function: Conversion of folates to polyglutamate derivatives [H]

COG id: COG0285

COG function: function code H; Folylpolyglutamate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the folylpolyglutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI66932984, Length=342, Percent_Identity=31.8713450292398, Blast_Score=127, Evalue=1e-29,
Organism=Homo sapiens, GI66932990, Length=342, Percent_Identity=31.8713450292398, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1788654, Length=430, Percent_Identity=43.0232558139535, Blast_Score=323, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI17553148, Length=478, Percent_Identity=28.6610878661088, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17553150, Length=473, Percent_Identity=28.9640591966173, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71984923, Length=479, Percent_Identity=28.6012526096033, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6323760, Length=363, Percent_Identity=28.9256198347107, Blast_Score=140, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6324815, Length=447, Percent_Identity=25.9507829977629, Blast_Score=106, Evalue=8e-24,
Organism=Drosophila melanogaster, GI24641571, Length=365, Percent_Identity=27.9452054794521, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24581568, Length=168, Percent_Identity=36.3095238095238, Blast_Score=107, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018109
- InterPro:   IPR001645
- InterPro:   IPR004101
- InterPro:   IPR013221 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.17; =6.3.2.12 [H]

Molecular weight: Translated: 48372; Mature: 48241

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFSFASATLPEWLDYLERLHPAAIEMGLERIRRVQAELELEPSFPIISVAGTNGKGST
CCCCCCHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCH
CMMLEAILSHAGYRVGCYTSPHLLRYNERVRIDRKEASDDELCEAFRAVESARMNSAVSL
HHHHHHHHHHCCCEEEECCCCHHEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEEEE
TYFEFGTLAAMYLFSQAEVEVAILEVGLGGRLDAVNVFEADCAVLTSVDFDHMDYLGNTR
EEHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCEEEEECCCHHHHCCCCHHHHHHHCCCH
EQIGFEKAGIFRSGKAAICSEPDLPISVRRHAESIGADLMHIGEHFGYSTAPQSWSYWRN
HHHCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCC
GESRHALPYPALRGAYQLKNASACLAALDSLKDTLPVTLSDIRHGLLEVVWPARFQVLPG
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEECCC
QPVRVLDVAHNPGAARALAASLDSMGRYPRTYAVFAMLGDKDIAGVVRELRSSVDVWLVS
CCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEE
GIDAPRGATADEAATQVAQALQIAEPLPGNAGEGAAHTIRKFRNPSEAYAYACEQAARND
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCC
RICVFGSFHTVAEVLRNRIERG
CEEEEECHHHHHHHHHHHHCCC
>Mature Secondary Structure 
STFSFASATLPEWLDYLERLHPAAIEMGLERIRRVQAELELEPSFPIISVAGTNGKGST
CCCCCHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCH
CMMLEAILSHAGYRVGCYTSPHLLRYNERVRIDRKEASDDELCEAFRAVESARMNSAVSL
HHHHHHHHHHCCCEEEECCCCHHEECCCCEEEECCCCCHHHHHHHHHHHHHHHCCCEEEE
TYFEFGTLAAMYLFSQAEVEVAILEVGLGGRLDAVNVFEADCAVLTSVDFDHMDYLGNTR
EEHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCEEEEECCCHHHHCCCCHHHHHHHCCCH
EQIGFEKAGIFRSGKAAICSEPDLPISVRRHAESIGADLMHIGEHFGYSTAPQSWSYWRN
HHHCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHCC
GESRHALPYPALRGAYQLKNASACLAALDSLKDTLPVTLSDIRHGLLEVVWPARFQVLPG
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEECCC
QPVRVLDVAHNPGAARALAASLDSMGRYPRTYAVFAMLGDKDIAGVVRELRSSVDVWLVS
CCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHCCCCEEEEE
GIDAPRGATADEAATQVAQALQIAEPLPGNAGEGAAHTIRKFRNPSEAYAYACEQAARND
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCC
RICVFGSFHTVAEVLRNRIERG
CEEEEECHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3040739; 3040734; 9205837; 9278503; 1989505 [H]