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The map label for this gene is purF [H]

Identifier: 82703027

GI number: 82703027

Start: 2196291

End: 2197811

Strand: Reverse

Name: purF [H]

Synonym: Nmul_A1906

Alternate gene names: 82703027

Gene position: 2197811-2196291 (Counterclockwise)

Preceding gene: 82703028

Following gene: 82703026

Centisome position: 69.02

GC content: 55.62

Gene sequence:

>1521_bases
ATGTGTGGAATTCTGGGTTTAGTTTCGCATGCTCCCGTCAACCAGTTACTCTACGATGGGTTGCTGGTGCTGCAGCATCG
TGGTCAGGATGCGGCCGGGATTGTTACCGCCCAGGGGAATACTTTCCACATGCACAAGAACAGCGGCCTCGTGCGCGACG
TGTTCCGCACGCGGAATATGCGCGCCTTGCTGGGGAATATGGGGATCGGCCAAGTGCGCTATCCTACCGCAGGTTCCGCC
GAGTCCCCTGCAGAAGCGCAGCCGTTTTATGTCAACTCACCCTTCGGAATCGTACTCGGGCATAACGGCAATCTGACCAA
TTCCGCACAGCTCAACGACGAGTTGTTCCGCGAGGATTTACGGCACGTCAACACCAATTCGGATTCGGAAGTGCTCCTTA
ATGTGCTGGCCCACGAGTTGCAGAGGAGCACGAAAAACCACCAGCTTGATCCCGCCGCTATTTTTGCCGCCGTATCCGGC
GTGCACGAGCGTTGCAAAGGCGCCTACGCCGTCATAGCAATGATTGCGGGTTACGGATTGCTCGCGTTCCGCGACCCTTA
TGGTATTCGCCCCCTTGTGTTCGGGTGCGCGGAAACGGAGCATGGGACGGAATATCTGGTAGCGTCCGAGAGTGTGGCGC
TCGATACTCTGGGATTCAAGCTGGTGAGGGATGTTGCGCCGGGCGAGGCAATTTTTATTGATGACACCGGCAATTTTTAC
AGTAAGCAGTGCGCGTCCAACCCCTCGCACAATCCCTGCATCTTCGAGTATGTCTATCTCGCACGCCCGGATTCAGTCAT
AGACGGAATTTCAGTCTATGAAACGCGGCTCAACATGGGAGAGAGTCTGGCCGATAAAGTCAAAGCCACGATGGGTCATC
TCGATATCGATGTAGTCATTCCGATTCCCGATTCCAGCCGTCCCAGCGCCTTGCAGCTTGCCAACCGCCTGGGCATAAGT
TTTCGTGAAGGTTTCGTAAAAAACCGCTACATCGGGCGAACCTTTATCATGCCGGGCCAGCAGTTGCGCCGTAAATCGGT
CCGCCAGAAGCTCAATGCCATGGGGGTGGAGTTTCGCGGAAAAAATGTGTTGCTGGTGGATGATTCGATCGTACGCGGCA
CTACCAGCCGCGAAATCGTCCAGATGGCGAAGGAAGCCGGTGCGAACAAGGTATTCTTTGCTTCTGCTGCCCCCCCCGTG
CGGTTTCCCAACGTTTATGGCATCGATATGCCTACGCGCCACGAACTCATTGCCACGGGGCGGGACGACGAGGAAATATG
CCGGGAAATTGGCGCCGATTATCTGGTGTATCAGGAACTCGATTCTCTCATTAAAGATGTGGGCCGTGTCAATCCGTCGA
TCTCGCAGTATGAGACCTCATGTTTCGACGGACGCTACATTACCGGGGATGTAACGCCGGAATACCTGGCCGTCCTGGAG
GCGCAACGCAGTGGCGGCGCTCCTCTTACCGAGAGACGTTCCAGCACGCAACTCGACCTGAATCTCGTGACAGCGAATTA
A

Upstream 100 bases:

>100_bases
AAGCAATAGGGCAGTAAACCGAATGCGCAGGATTCCACTCACTGAACTCGTGCAACTTTTCGTATAGCGTCGATCTAAGT
CATTTTTGCGGAGTGCATTC

Downstream 100 bases:

>100_bases
TGAAGATTTGACAAAAGCAGGTACCGAGCGTACCTTCAGGGCAGGCGCCGATTTGATCTTCAGCTTGCGGGCGCAGTATA
AATTCAGCTAAAGCGGGAAA

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MCGILGLVSHAPVNQLLYDGLLVLQHRGQDAAGIVTAQGNTFHMHKNSGLVRDVFRTRNMRALLGNMGIGQVRYPTAGSA
ESPAEAQPFYVNSPFGIVLGHNGNLTNSAQLNDELFREDLRHVNTNSDSEVLLNVLAHELQRSTKNHQLDPAAIFAAVSG
VHERCKGAYAVIAMIAGYGLLAFRDPYGIRPLVFGCAETEHGTEYLVASESVALDTLGFKLVRDVAPGEAIFIDDTGNFY
SKQCASNPSHNPCIFEYVYLARPDSVIDGISVYETRLNMGESLADKVKATMGHLDIDVVIPIPDSSRPSALQLANRLGIS
FREGFVKNRYIGRTFIMPGQQLRRKSVRQKLNAMGVEFRGKNVLLVDDSIVRGTTSREIVQMAKEAGANKVFFASAAPPV
RFPNVYGIDMPTRHELIATGRDDEEICREIGADYLVYQELDSLIKDVGRVNPSISQYETSCFDGRYITGDVTPEYLAVLE
AQRSGGAPLTERRSSTQLDLNLVTAN

Sequences:

>Translated_506_residues
MCGILGLVSHAPVNQLLYDGLLVLQHRGQDAAGIVTAQGNTFHMHKNSGLVRDVFRTRNMRALLGNMGIGQVRYPTAGSA
ESPAEAQPFYVNSPFGIVLGHNGNLTNSAQLNDELFREDLRHVNTNSDSEVLLNVLAHELQRSTKNHQLDPAAIFAAVSG
VHERCKGAYAVIAMIAGYGLLAFRDPYGIRPLVFGCAETEHGTEYLVASESVALDTLGFKLVRDVAPGEAIFIDDTGNFY
SKQCASNPSHNPCIFEYVYLARPDSVIDGISVYETRLNMGESLADKVKATMGHLDIDVVIPIPDSSRPSALQLANRLGIS
FREGFVKNRYIGRTFIMPGQQLRRKSVRQKLNAMGVEFRGKNVLLVDDSIVRGTTSREIVQMAKEAGANKVFFASAAPPV
RFPNVYGIDMPTRHELIATGRDDEEICREIGADYLVYQELDSLIKDVGRVNPSISQYETSCFDGRYITGDVTPEYLAVLE
AQRSGGAPLTERRSSTQLDLNLVTAN
>Mature_506_residues
MCGILGLVSHAPVNQLLYDGLLVLQHRGQDAAGIVTAQGNTFHMHKNSGLVRDVFRTRNMRALLGNMGIGQVRYPTAGSA
ESPAEAQPFYVNSPFGIVLGHNGNLTNSAQLNDELFREDLRHVNTNSDSEVLLNVLAHELQRSTKNHQLDPAAIFAAVSG
VHERCKGAYAVIAMIAGYGLLAFRDPYGIRPLVFGCAETEHGTEYLVASESVALDTLGFKLVRDVAPGEAIFIDDTGNFY
SKQCASNPSHNPCIFEYVYLARPDSVIDGISVYETRLNMGESLADKVKATMGHLDIDVVIPIPDSSRPSALQLANRLGIS
FREGFVKNRYIGRTFIMPGQQLRRKSVRQKLNAMGVEFRGKNVLLVDDSIVRGTTSREIVQMAKEAGANKVFFASAAPPV
RFPNVYGIDMPTRHELIATGRDDEEICREIGADYLVYQELDSLIKDVGRVNPSISQYETSCFDGRYITGDVTPEYLAVLE
AQRSGGAPLTERRSSTQLDLNLVTAN

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=474, Percent_Identity=36.7088607594937, Blast_Score=281, Evalue=8e-76,
Organism=Escherichia coli, GI1788651, Length=499, Percent_Identity=59.9198396793587, Blast_Score=601, Evalue=1e-173,
Organism=Escherichia coli, GI1790167, Length=218, Percent_Identity=29.3577981651376, Blast_Score=65, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17554892, Length=482, Percent_Identity=34.2323651452282, Blast_Score=254, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6323958, Length=492, Percent_Identity=51.6260162601626, Blast_Score=477, Evalue=1e-135,
Organism=Drosophila melanogaster, GI24659598, Length=506, Percent_Identity=34.9802371541502, Blast_Score=272, Evalue=5e-73,
Organism=Drosophila melanogaster, GI24659604, Length=482, Percent_Identity=36.0995850622407, Blast_Score=269, Evalue=3e-72,
Organism=Drosophila melanogaster, GI28573187, Length=498, Percent_Identity=33.3333333333333, Blast_Score=262, Evalue=3e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55455; Mature: 55455

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II ; PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGILGLVSHAPVNQLLYDGLLVLQHRGQDAAGIVTAQGNTFHMHKNSGLVRDVFRTRNM
CCCHHHHHCCCCHHHHHHHCCCEEEECCCCCCEEEEECCCEEEEECCCCHHHHHHHHCCH
RALLGNMGIGQVRYPTAGSAESPAEAQPFYVNSPFGIVLGHNGNLTNSAQLNDELFREDL
HHHHHCCCCCEEECCCCCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCCCCHHHHHHHH
RHVNTNSDSEVLLNVLAHELQRSTKNHQLDPAAIFAAVSGVHERCKGAYAVIAMIAGYGL
HHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
LAFRDPYGIRPLVFGCAETEHGTEYLVASESVALDTLGFKLVRDVAPGEAIFIDDTGNFY
EEECCCCCCCCEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCHH
SKQCASNPSHNPCIFEYVYLARPDSVIDGISVYETRLNMGESLADKVKATMGHLDIDVVI
HHHHCCCCCCCCEEEEEEEEECCCHHHCCHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEE
PIPDSSRPSALQLANRLGISFREGFVKNRYIGRTFIMPGQQLRRKSVRQKLNAMGVEFRG
ECCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCEEECC
KNVLLVDDSIVRGTTSREIVQMAKEAGANKVFFASAAPPVRFPNVYGIDMPTRHELIATG
CEEEEECCHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCEEECC
RDDEEICREIGADYLVYQELDSLIKDVGRVNPSISQYETSCFDGRYITGDVTPEYLAVLE
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEECCCCHHHHHHHH
AQRSGGAPLTERRSSTQLDLNLVTAN
HHCCCCCCCHHCCCCCEEEEEEEECC
>Mature Secondary Structure
MCGILGLVSHAPVNQLLYDGLLVLQHRGQDAAGIVTAQGNTFHMHKNSGLVRDVFRTRNM
CCCHHHHHCCCCHHHHHHHCCCEEEECCCCCCEEEEECCCEEEEECCCCHHHHHHHHCCH
RALLGNMGIGQVRYPTAGSAESPAEAQPFYVNSPFGIVLGHNGNLTNSAQLNDELFREDL
HHHHHCCCCCEEECCCCCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCCCCHHHHHHHH
RHVNTNSDSEVLLNVLAHELQRSTKNHQLDPAAIFAAVSGVHERCKGAYAVIAMIAGYGL
HHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCE
LAFRDPYGIRPLVFGCAETEHGTEYLVASESVALDTLGFKLVRDVAPGEAIFIDDTGNFY
EEECCCCCCCCEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCHH
SKQCASNPSHNPCIFEYVYLARPDSVIDGISVYETRLNMGESLADKVKATMGHLDIDVVI
HHHHCCCCCCCCEEEEEEEEECCCHHHCCHHHHHHHHHCCHHHHHHHHHHHCCCEEEEEE
PIPDSSRPSALQLANRLGISFREGFVKNRYIGRTFIMPGQQLRRKSVRQKLNAMGVEFRG
ECCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCEEECC
KNVLLVDDSIVRGTTSREIVQMAKEAGANKVFFASAAPPVRFPNVYGIDMPTRHELIATG
CEEEEECCHHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCEEECC
RDDEEICREIGADYLVYQELDSLIKDVGRVNPSISQYETSCFDGRYITGDVTPEYLAVLE
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEECCCCHHHHHHHH
AQRSGGAPLTERRSSTQLDLNLVTAN
HHCCCCCCCHHCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]