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The map label for this gene is metZ [H]

Identifier: 82703026

GI number: 82703026

Start: 2194951

End: 2196138

Strand: Reverse

Name: metZ [H]

Synonym: Nmul_A1905

Alternate gene names: 82703026

Gene position: 2196138-2194951 (Counterclockwise)

Preceding gene: 82703027

Following gene: 82703021

Centisome position: 68.97

GC content: 58.25

Gene sequence:

>1188_bases
ATGTCGGGCATGTCTGACGATTTTGAGCTGGAAACACTGGCGCTGCATACGGGCATACATCGCAGCCAATTCAATGAACA
CTCGGAGGCCTTATTCCTGACTTCGAGTTTCGTCTTCGACAGTGCCGCTCAGGCGGCAGCACGTTTTTCCGATGCGGAAC
CGGGTAACATCTATTCGCGTTTTACCAACCCCACGGTTACCGCTTTTCAGGAAAGACTGGCCGCCCTGGAGGGGGCGGAA
GCCTGTGTCGCCACCTCCTCAGGCATGTCGGCCATTCTTGCCTGCACGATGGGTCTCCTCCGTGCTGGAGACCATATCGT
GGCTTCGCGCAGCCTGTTCGGTGCGACCGTCAGCCTGTTCAACAATATCCTCAAGCGCTTCAACATCGAAACGACGTTTG
TTTCCGCTACGGATGTTTCCGCCTGGGAAGCAGCGGTGAGACCAGCCACCCGGTTGCTGTTCATGGAAACGCCCTCCAAT
CCGCTGACAGAGATTTCCGACATTGCCGCGCTTGCAGCCGTAGCCAGGAAGGCGGGCGCGTGGCTCGCCGTGGACAATTG
TTTCTGCTCTCCCGCGCTGCAACGTCCATTGGAATGGGGCGCAGACCTCGTGATTCACTCTGCAACCAAATATCTTGAGG
GCCAGGGCCGGGTATTGGGTGGGGCGGTGCTGGGCAAGCGCGATCTGGTGATGGACGGGGGAATATTCGGTTTTCTGCGC
ACTGCCGGACCGACCCTGAGCCCGTTCAATGCCTGGGTGATCCTGAAGGGCATGGAAACCCTGAACCTTCGGATGGAAAG
GCATTCCGAAAATGCGCTGGAAATTGCGCGCTGGCTGGAAGCCCAGCCCGGCGTAGCCAAAGTCCACTATCCCGGCTTGC
CATCGCATCCGCAGTACGAGCTTGCCAGCCGCCAGCAGAAAACCGGGGGGGGAATCGTTTCATTCGAATTGAAGAATGGA
AGAGAAGCGGCATGGAGGGTGGTGGATTCTGTTCGCATGATTTCCATCACCGCCAACCTGGGAGATGCCAAGAGCACCCT
TACCCATCCTGCCACCACCACGCATGGGCGGATCAGCCAGGAAAACCGGGATGCGGCTGGCATTACCGAGGGCTTGCTGC
GCATTGCAGTGGGACTTGAAGCGGTAAAAGACATAAAGGCCGATCTCGCAAGGGGCTTGACCCGGTAG

Upstream 100 bases:

>100_bases
TTGATCTTCAGCTTGCGGGCGCAGTATAAATTCAGCTAAAGCGGGAAAACCCGTTTTTTAGCGAAAGCTGAACGGGTTTT
TTATTGGGAATTTGAGGGAG

Downstream 100 bases:

>100_bases
AGGGGAGTAAGGGAATACGTGCCTTCAAGGGCGCTATCGCCCTGGACGCCCTGGATTAGGTTAGCCGGAGGGGTTTCCTT
TCAGTTCCGCATTATCGGCC

Product: O-succinylhomoserine sulfhydrylase

Products: Cystathionine; succinate; o-succinyl-L-homoserine; H2O [C]

Alternate protein names: OSH sulfhydrylase [H]

Number of amino acids: Translated: 395; Mature: 394

Protein sequence:

>395_residues
MSGMSDDFELETLALHTGIHRSQFNEHSEALFLTSSFVFDSAAQAAARFSDAEPGNIYSRFTNPTVTAFQERLAALEGAE
ACVATSSGMSAILACTMGLLRAGDHIVASRSLFGATVSLFNNILKRFNIETTFVSATDVSAWEAAVRPATRLLFMETPSN
PLTEISDIAALAAVARKAGAWLAVDNCFCSPALQRPLEWGADLVIHSATKYLEGQGRVLGGAVLGKRDLVMDGGIFGFLR
TAGPTLSPFNAWVILKGMETLNLRMERHSENALEIARWLEAQPGVAKVHYPGLPSHPQYELASRQQKTGGGIVSFELKNG
REAAWRVVDSVRMISITANLGDAKSTLTHPATTTHGRISQENRDAAGITEGLLRIAVGLEAVKDIKADLARGLTR

Sequences:

>Translated_395_residues
MSGMSDDFELETLALHTGIHRSQFNEHSEALFLTSSFVFDSAAQAAARFSDAEPGNIYSRFTNPTVTAFQERLAALEGAE
ACVATSSGMSAILACTMGLLRAGDHIVASRSLFGATVSLFNNILKRFNIETTFVSATDVSAWEAAVRPATRLLFMETPSN
PLTEISDIAALAAVARKAGAWLAVDNCFCSPALQRPLEWGADLVIHSATKYLEGQGRVLGGAVLGKRDLVMDGGIFGFLR
TAGPTLSPFNAWVILKGMETLNLRMERHSENALEIARWLEAQPGVAKVHYPGLPSHPQYELASRQQKTGGGIVSFELKNG
REAAWRVVDSVRMISITANLGDAKSTLTHPATTTHGRISQENRDAAGITEGLLRIAVGLEAVKDIKADLARGLTR
>Mature_394_residues
SGMSDDFELETLALHTGIHRSQFNEHSEALFLTSSFVFDSAAQAAARFSDAEPGNIYSRFTNPTVTAFQERLAALEGAEA
CVATSSGMSAILACTMGLLRAGDHIVASRSLFGATVSLFNNILKRFNIETTFVSATDVSAWEAAVRPATRLLFMETPSNP
LTEISDIAALAAVARKAGAWLAVDNCFCSPALQRPLEWGADLVIHSATKYLEGQGRVLGGAVLGKRDLVMDGGIFGFLRT
AGPTLSPFNAWVILKGMETLNLRMERHSENALEIARWLEAQPGVAKVHYPGLPSHPQYELASRQQKTGGGIVSFELKNGR
EAAWRVVDSVRMISITANLGDAKSTLTHPATTTHGRISQENRDAAGITEGLLRIAVGLEAVKDIKADLARGLTR

Specific function: Converts O-succinylhomoserine into homocysteine [H]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=336, Percent_Identity=36.9047619047619, Blast_Score=234, Evalue=9e-62,
Organism=Homo sapiens, GI299473759, Length=336, Percent_Identity=33.3333333333333, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI25453487, Length=335, Percent_Identity=31.3432835820896, Blast_Score=179, Evalue=4e-45,
Organism=Escherichia coli, GI1790375, Length=384, Percent_Identity=38.0208333333333, Blast_Score=248, Evalue=5e-67,
Organism=Escherichia coli, GI1789383, Length=373, Percent_Identity=28.4182305630027, Blast_Score=142, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17538055, Length=374, Percent_Identity=36.8983957219251, Blast_Score=248, Evalue=4e-66,
Organism=Caenorhabditis elegans, GI17560056, Length=326, Percent_Identity=38.9570552147239, Blast_Score=241, Evalue=7e-64,
Organism=Caenorhabditis elegans, GI71980708, Length=403, Percent_Identity=32.258064516129, Blast_Score=213, Evalue=1e-55,
Organism=Saccharomyces cerevisiae, GI6323333, Length=431, Percent_Identity=35.2668213457077, Blast_Score=234, Evalue=2e-62,
Organism=Saccharomyces cerevisiae, GI6319307, Length=399, Percent_Identity=34.5864661654135, Blast_Score=223, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6321254, Length=404, Percent_Identity=27.4752475247525, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6321170, Length=346, Percent_Identity=26.5895953757225, Blast_Score=132, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6321904, Length=355, Percent_Identity=27.0422535211268, Blast_Score=101, Evalue=3e-22,
Organism=Drosophila melanogaster, GI19922554, Length=386, Percent_Identity=34.7150259067358, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006234
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: 2.5.1.48 [C]

Molecular weight: Translated: 42471; Mature: 42340

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGMSDDFELETLALHTGIHRSQFNEHSEALFLTSSFVFDSAAQAAARFSDAEPGNIYSR
CCCCCCCCCCEEEHHHHCCHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCHHHH
FTNPTVTAFQERLAALEGAEACVATSSGMSAILACTMGLLRAGDHIVASRSLFGATVSLF
CCCCHHHHHHHHHHHHCCCHHEEECCCCHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHH
NNILKRFNIETTFVSATDVSAWEAAVRPATRLLFMETPSNPLTEISDIAALAAVARKAGA
HHHHHHCCCCEEEEECCCCHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCC
WLAVDNCFCSPALQRPLEWGADLVIHSATKYLEGQGRVLGGAVLGKRDLVMDGGIFGFLR
EEEECCCCCCHHHHCHHHHCCCEEEEHHHHHHCCCCCEEEEEEECCCCEEECCCHHHHHH
TAGPTLSPFNAWVILKGMETLNLRMERHSENALEIARWLEAQPGVAKVHYPGLPSHPQYE
HCCCCCCCCCEEEEEECCHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHH
LASRQQKTGGGIVSFELKNGREAAWRVVDSVRMISITANLGDAKSTLTHPATTTHGRISQ
HHHHHHHCCCCEEEEEECCCCHHHHHHHHCEEEEEEEECCCCCHHHCCCCCCCCCCCCCC
ENRDAAGITEGLLRIAVGLEAVKDIKADLARGLTR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SGMSDDFELETLALHTGIHRSQFNEHSEALFLTSSFVFDSAAQAAARFSDAEPGNIYSR
CCCCCCCCCEEEHHHHCCHHHHCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCHHHH
FTNPTVTAFQERLAALEGAEACVATSSGMSAILACTMGLLRAGDHIVASRSLFGATVSLF
CCCCHHHHHHHHHHHHCCCHHEEECCCCHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHH
NNILKRFNIETTFVSATDVSAWEAAVRPATRLLFMETPSNPLTEISDIAALAAVARKAGA
HHHHHHCCCCEEEEECCCCHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCC
WLAVDNCFCSPALQRPLEWGADLVIHSATKYLEGQGRVLGGAVLGKRDLVMDGGIFGFLR
EEEECCCCCCHHHHCHHHHCCCEEEEHHHHHHCCCCCEEEEEEECCCCEEECCCHHHHHH
TAGPTLSPFNAWVILKGMETLNLRMERHSENALEIARWLEAQPGVAKVHYPGLPSHPQYE
HCCCCCCCCCEEEEEECCHHHHHHHHHCCCHHHHHHHHHCCCCCEEEEECCCCCCCCCHH
LASRQQKTGGGIVSFELKNGREAAWRVVDSVRMISITANLGDAKSTLTHPATTTHGRISQ
HHHHHHHCCCCEEEEEECCCCHHHHHHHHCEEEEEEEECCCCCHHHCCCCCCCCCCCCCC
ENRDAAGITEGLLRIAVGLEAVKDIKADLARGLTR
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): 460 [C]

Specific activity: 10

Km value (mM): 0.33 {O-succinyl-L-homoserine}} [C]

Substrates: O-succinyl-L-homoserine; @CYST01.txt*L-cysteine!; 2-oxobutanoate; succinate; NH3 [C]

Specific reaction: O-succinyl-L-homoserine + @CYST01.txt*L-cysteine! = cystathionine + succinate 2-oxobutanoate + succinate + NH3 = o-succinyl-L-homoserine + H2O [C]

General reaction: Elimination; Gamma-replacement [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704274; 10984043 [H]