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The map label for this gene is rluB [H]

Identifier: 82702976

GI number: 82702976

Start: 2138266

End: 2139213

Strand: Reverse

Name: rluB [H]

Synonym: Nmul_A1855

Alternate gene names: 82702976

Gene position: 2139213-2138266 (Counterclockwise)

Preceding gene: 82702977

Following gene: 82702974

Centisome position: 67.18

GC content: 58.12

Gene sequence:

>948_bases
ATGAAAACCAGGTCATCGCCGTCCCCCGAGGCGGTCAGACGACGCCGTTCCATGAAAATCACAAAGTCTCCCGCTTCGTC
AACTCCGGCAGACTCACCCAAATCACTCCAATCGCCTGTACGAAGCCGGCTGAGAGGAGCGGGGCTAAAGGGATCGATAA
AACCCGCGGCAACACCTCAATCTGGCGTGGGTTCCACCTCCCCTCAACCCGTTACGCAGAAATTGCACAAGGTGCTGGCC
CAGGCGGGATTGGGTTCGAGACGCGAAATGGAAGAGCTGATCGCCAGTGGACAGGTGACGATCAACGGAAAGACCGCACA
AGTGGGGGACCGTATCGCCGCTCGAGATGTCGTGAGAGTAGGGAAGCGCGTCGTTCACTTCAGATCCGCCCGCCGTCTGC
CCCGCGTCATGCTCTATCACAAGCCGGAGGGGGAAATCGTCAGCCAGGATGATCCCCAAGGCCGTACGTCGGTCTTCGAC
AAACTGCCGCAGTTGAGGTCGTCCAAATGGATTGCTATTGGCAGGTTGGATTACAACACCAGCGGTTTGCTGGTTTTCAC
CACGGATGGGGAATTGGCCAACCGGCTGATGCATCCGCGCTTCGAAGTCGAGCGCGAATACGCAGTGCGGATCATCGGAA
GGCTGACGCCGGAGCAGGCAACGTTGCTGACCTCGGGTGTTGAGCTGGAGGACGGCGCGGCCAAGTTCGATTACCTGTCG
GATGAAGGGGGTGAGGGCTCCAACCACTGGTATCGCGTGATATTGAAGGAAGGCAGGAATCGCGAAGTACGGCGGATGTT
CGAAGCGGTGGGAATGACGGTGAGCCGCCTGATGCGGGTTCGTTTTGGCCCTATCAACTTGCCCTCCCGTTTGAAACGGG
GAAAGTCGATGGAATTGAACGAGGCAGAGATAGGACGCCTGCTGGAACTGGTCGGTTACAAAGCCTGA

Upstream 100 bases:

>100_bases
ACGAAGGTTCAGTACAAACAGCCCACCGTTTTTCTCATCCCATCCGAGCACTCGCTAGCATGGGAAACTGGAAATTGGAA
GGCTTGAAATTGGAGCTGCG

Downstream 100 bases:

>100_bases
TGCCTGGGAAGCTGGTCCCGTTTCAGCAGGAAAACAAACTCATCGCCTGCACTGGTGTCCAGCCAGGTAAAAGGTGTATC
GGGAAAGGCACGCTCCAGGG

Product: pseudouridine synthase Rsu

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MKTRSSPSPEAVRRRRSMKITKSPASSTPADSPKSLQSPVRSRLRGAGLKGSIKPAATPQSGVGSTSPQPVTQKLHKVLA
QAGLGSRREMEELIASGQVTINGKTAQVGDRIAARDVVRVGKRVVHFRSARRLPRVMLYHKPEGEIVSQDDPQGRTSVFD
KLPQLRSSKWIAIGRLDYNTSGLLVFTTDGELANRLMHPRFEVEREYAVRIIGRLTPEQATLLTSGVELEDGAAKFDYLS
DEGGEGSNHWYRVILKEGRNREVRRMFEAVGMTVSRLMRVRFGPINLPSRLKRGKSMELNEAEIGRLLELVGYKA

Sequences:

>Translated_315_residues
MKTRSSPSPEAVRRRRSMKITKSPASSTPADSPKSLQSPVRSRLRGAGLKGSIKPAATPQSGVGSTSPQPVTQKLHKVLA
QAGLGSRREMEELIASGQVTINGKTAQVGDRIAARDVVRVGKRVVHFRSARRLPRVMLYHKPEGEIVSQDDPQGRTSVFD
KLPQLRSSKWIAIGRLDYNTSGLLVFTTDGELANRLMHPRFEVEREYAVRIIGRLTPEQATLLTSGVELEDGAAKFDYLS
DEGGEGSNHWYRVILKEGRNREVRRMFEAVGMTVSRLMRVRFGPINLPSRLKRGKSMELNEAEIGRLLELVGYKA
>Mature_315_residues
MKTRSSPSPEAVRRRRSMKITKSPASSTPADSPKSLQSPVRSRLRGAGLKGSIKPAATPQSGVGSTSPQPVTQKLHKVLA
QAGLGSRREMEELIASGQVTINGKTAQVGDRIAARDVVRVGKRVVHFRSARRLPRVMLYHKPEGEIVSQDDPQGRTSVFD
KLPQLRSSKWIAIGRLDYNTSGLLVFTTDGELANRLMHPRFEVEREYAVRIIGRLTPEQATLLTSGVELEDGAAKFDYLS
DEGGEGSNHWYRVILKEGRNREVRRMFEAVGMTVSRLMRVRFGPINLPSRLKRGKSMELNEAEIGRLLELVGYKA

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=244, Percent_Identity=54.9180327868852, Blast_Score=268, Evalue=3e-73,
Organism=Escherichia coli, GI1790453, Length=238, Percent_Identity=31.5126050420168, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788510, Length=180, Percent_Identity=32.2222222222222, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI87081838, Length=200, Percent_Identity=32.5, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 34951; Mature: 34951

Theoretical pI: Translated: 11.26; Mature: 11.26

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTRSSPSPEAVRRRRSMKITKSPASSTPADSPKSLQSPVRSRLRGAGLKGSIKPAATPQ
CCCCCCCCHHHHHHHHHCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
SGVGSTSPQPVTQKLHKVLAQAGLGSRREMEELIASGQVTINGKTAQVGDRIAARDVVRV
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHH
GKRVVHFRSARRLPRVMLYHKPEGEIVSQDDPQGRTSVFDKLPQLRSSKWIAIGRLDYNT
HHHHHHHHHHHCCCEEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCCEEEEEEEEECC
SGLLVFTTDGELANRLMHPRFEVEREYAVRIIGRLTPEQATLLTSGVELEDGAAKFDYLS
CCEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCEECCCCCHHCCCC
DEGGEGSNHWYRVILKEGRNREVRRMFEAVGMTVSRLMRVRFGPINLPSRLKRGKSMELN
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCC
EAEIGRLLELVGYKA
HHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTRSSPSPEAVRRRRSMKITKSPASSTPADSPKSLQSPVRSRLRGAGLKGSIKPAATPQ
CCCCCCCCHHHHHHHHHCEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
SGVGSTSPQPVTQKLHKVLAQAGLGSRREMEELIASGQVTINGKTAQVGDRIAARDVVRV
CCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHH
GKRVVHFRSARRLPRVMLYHKPEGEIVSQDDPQGRTSVFDKLPQLRSSKWIAIGRLDYNT
HHHHHHHHHHHCCCEEEEEECCCCCEECCCCCCCHHHHHHHHHHHCCCCEEEEEEEEECC
SGLLVFTTDGELANRLMHPRFEVEREYAVRIIGRLTPEQATLLTSGVELEDGAAKFDYLS
CCEEEEECCHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCEECCCCCHHCCCC
DEGGEGSNHWYRVILKEGRNREVRRMFEAVGMTVSRLMRVRFGPINLPSRLKRGKSMELN
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCC
EAEIGRLLELVGYKA
HHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]