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The map label for this gene is hprA [H]

Identifier: 82702969

GI number: 82702969

Start: 2131652

End: 2132602

Strand: Reverse

Name: hprA [H]

Synonym: Nmul_A1848

Alternate gene names: 82702969

Gene position: 2132602-2131652 (Counterclockwise)

Preceding gene: 82702970

Following gene: 82702968

Centisome position: 66.97

GC content: 55.63

Gene sequence:

>951_bases
ATGAATCATCACGTCGTTTTTCTGGAAAGGGATTCCCTGAAGGTGGAAGTGCGCCGGCCTAATTTCGAGCACACGTATGA
GGAGTACGCCAAAACGAAGCCTGACCAGGTGGTGTCGCATTTACAGCATGCAACCATCGCAATCATCAACAAGGTGGGGT
TGAGCGAGGAAACTCTGGCACAATTGCCAAAGCTGAAAATGGTCGCCATTGCGGCCACCGGATATGACGCTGTCGATATC
GGTGCTTGTCGCCGACACGGCATTGCAGTGGCGAATATCCGCAATTATGCAATTCATACCGTGCCTGAGCATGTATTCGC
CCTGGTGCTCGCGCTACGCAGAAATCTTTTAGCCTATCGACAGGAGGTCGAGCAGGGAAGGTGGCAGAAATCGGAGCAGT
TCTGCCTGTTTACTCATGAGATCCGTGAGTTGTTTGGCGCCACCATCGGTATTGTCGGGGAGGGCACGATCGGGCGCGGT
ACCGCCGCCATTGCACAGGGATTCGGCATGCGCGTGCTGTTTGCCCATCATGAATCGCGAAAGAAGAAGGACATGGTTCT
TACCCCCTTCGATCAGGTACTTGCGGAATCCGACATCCTCTCGCTGCATTGCCCGCTGACCCCCGCTACCCGAAACCTGA
TTGGCGTGAACGAATTGCGCAAGATGAAGCGCAGCGCATTGCTCATCAACACGGCGCGGGGAGGATTGGTGGATGAGGCG
GCATTGGTACAGGCTCTGGACGATGGCACTATCGCCGGCGCCGGTTTCGATGTGCTGACGACGGAACCTCCCAGAAACGG
CAATCCCCTGCTTGGCTTGCGCCGTCCCAACTTTATCCTGACACCTCATATTGCTTGGGCCTCTACCGAGGCCATGCGTT
TTCTTGCCGATCAGCTGGTCGACAATATCGAAGCCTGGGTGGCAGGCAAGCCGCAAAACCTGCTTACCTGA

Upstream 100 bases:

>100_bases
CGAGGATGAACCGGGAGTAATACCGTCCACACCGGTTCTGCCGGTACTGGTACCGGAATTCCGGTTTCCTGTACAAATTT
CACACAAGAGGGAAATGAAC

Downstream 100 bases:

>100_bases
ATTTTCAGTCCGGTTCGCATCGGCGGAAAGGCGGAATATAAAGAACGCATATAGGCGGTTGACAACTCAAGTAGCCTTCG
ATAGTCTCATAGAGCTAGAG

Product: D-isomer specific 2-hydroxyacid dehydrogenase

Products: NA

Alternate protein names: GDH; Glyoxylate reductase; Hydroxypyruvate dehydrogenase; NADH-dependent hydroxypyruvate reductase; HPR; HPR-A [H]

Number of amino acids: Translated: 316; Mature: 316

Protein sequence:

>316_residues
MNHHVVFLERDSLKVEVRRPNFEHTYEEYAKTKPDQVVSHLQHATIAIINKVGLSEETLAQLPKLKMVAIAATGYDAVDI
GACRRHGIAVANIRNYAIHTVPEHVFALVLALRRNLLAYRQEVEQGRWQKSEQFCLFTHEIRELFGATIGIVGEGTIGRG
TAAIAQGFGMRVLFAHHESRKKKDMVLTPFDQVLAESDILSLHCPLTPATRNLIGVNELRKMKRSALLINTARGGLVDEA
ALVQALDDGTIAGAGFDVLTTEPPRNGNPLLGLRRPNFILTPHIAWASTEAMRFLADQLVDNIEAWVAGKPQNLLT

Sequences:

>Translated_316_residues
MNHHVVFLERDSLKVEVRRPNFEHTYEEYAKTKPDQVVSHLQHATIAIINKVGLSEETLAQLPKLKMVAIAATGYDAVDI
GACRRHGIAVANIRNYAIHTVPEHVFALVLALRRNLLAYRQEVEQGRWQKSEQFCLFTHEIRELFGATIGIVGEGTIGRG
TAAIAQGFGMRVLFAHHESRKKKDMVLTPFDQVLAESDILSLHCPLTPATRNLIGVNELRKMKRSALLINTARGGLVDEA
ALVQALDDGTIAGAGFDVLTTEPPRNGNPLLGLRRPNFILTPHIAWASTEAMRFLADQLVDNIEAWVAGKPQNLLT
>Mature_316_residues
MNHHVVFLERDSLKVEVRRPNFEHTYEEYAKTKPDQVVSHLQHATIAIINKVGLSEETLAQLPKLKMVAIAATGYDAVDI
GACRRHGIAVANIRNYAIHTVPEHVFALVLALRRNLLAYRQEVEQGRWQKSEQFCLFTHEIRELFGATIGIVGEGTIGRG
TAAIAQGFGMRVLFAHHESRKKKDMVLTPFDQVLAESDILSLHCPLTPATRNLIGVNELRKMKRSALLINTARGGLVDEA
ALVQALDDGTIAGAGFDVLTTEPPRNGNPLLGLRRPNFILTPHIAWASTEAMRFLADQLVDNIEAWVAGKPQNLLT

Specific function: Plays a central role in assimilation of carbon. It converts hydroxypyruvate to glycerate as a key step in the serine cycle, and may also play an important role in C2 reactions, by interconverting glyoxylate and glycolate [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI145580578, Length=274, Percent_Identity=33.5766423357664, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4557499, Length=274, Percent_Identity=33.5766423357664, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI145580575, Length=276, Percent_Identity=34.4202898550725, Blast_Score=130, Evalue=1e-30,
Organism=Homo sapiens, GI61743967, Length=275, Percent_Identity=32, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI4557497, Length=275, Percent_Identity=32, Blast_Score=129, Evalue=4e-30,
Organism=Homo sapiens, GI23308577, Length=281, Percent_Identity=29.8932384341637, Blast_Score=128, Evalue=7e-30,
Organism=Homo sapiens, GI6912396, Length=254, Percent_Identity=31.1023622047244, Blast_Score=116, Evalue=3e-26,
Organism=Escherichia coli, GI87082289, Length=257, Percent_Identity=34.2412451361868, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1789279, Length=269, Percent_Identity=32.3420074349442, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1787645, Length=305, Percent_Identity=27.5409836065574, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI87081824, Length=188, Percent_Identity=31.3829787234043, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17532191, Length=300, Percent_Identity=30.3333333333333, Blast_Score=136, Evalue=2e-32,
Organism=Caenorhabditis elegans, GI25147481, Length=284, Percent_Identity=29.5774647887324, Blast_Score=115, Evalue=3e-26,
Organism=Saccharomyces cerevisiae, GI6320925, Length=279, Percent_Identity=29.7491039426523, Blast_Score=121, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6322116, Length=292, Percent_Identity=29.4520547945205, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324055, Length=263, Percent_Identity=31.1787072243346, Blast_Score=110, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6324964, Length=288, Percent_Identity=25.3472222222222, Blast_Score=94, Evalue=2e-20,
Organism=Drosophila melanogaster, GI19921140, Length=252, Percent_Identity=29.7619047619048, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI28571528, Length=321, Percent_Identity=31.4641744548287, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24585516, Length=252, Percent_Identity=31.7460317460317, Blast_Score=124, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24646446, Length=270, Percent_Identity=32.2222222222222, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24646448, Length=270, Percent_Identity=32.2222222222222, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24646452, Length=270, Percent_Identity=32.2222222222222, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24646450, Length=270, Percent_Identity=32.2222222222222, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI62472511, Length=270, Percent_Identity=32.2222222222222, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI28574286, Length=255, Percent_Identity=28.6274509803922, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI45552429, Length=255, Percent_Identity=29.0196078431373, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24585514, Length=255, Percent_Identity=29.0196078431373, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI28574282, Length=255, Percent_Identity=29.0196078431373, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45551003, Length=255, Percent_Identity=29.0196078431373, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI28574284, Length=255, Percent_Identity=29.0196078431373, Blast_Score=108, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.29 [H]

Molecular weight: Translated: 35011; Mature: 35011

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHHVVFLERDSLKVEVRRPNFEHTYEEYAKTKPDQVVSHLQHATIAIINKVGLSEETLA
CCCEEEEEECCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
QLPKLKMVAIAATGYDAVDIGACRRHGIAVANIRNYAIHTVPEHVFALVLALRRNLLAYR
HCCCEEEEEEEECCCCCEEHHHHHHCCEEEEEECCCEEHHHHHHHHHHHHHHHHHHHHHH
QEVEQGRWQKSEQFCLFTHEIRELFGATIGIVGEGTIGRGTAAIAQGFGMRVLFAHHESR
HHHHHHCCCCCCCEEEHHHHHHHHHCCEEEEEECCCCCCCHHHHHHCCCEEEEEEECCCC
KKKDMVLTPFDQVLAESDILSLHCPLTPATRNLIGVNELRKMKRSALLINTARGGLVDEA
CCCCCEECCHHHHHCCCCEEEEECCCCHHHHCCCCHHHHHHHHHHHEEEECCCCCCCHHH
ALVQALDDGTIAGAGFDVLTTEPPRNGNPLLGLRRPNFILTPHIAWASTEAMRFLADQLV
HHHHHHCCCEEECCCCEEEECCCCCCCCCEEEECCCCEEEECCHHHHHHHHHHHHHHHHH
DNIEAWVAGKPQNLLT
HHHHHHHCCCCHHCCC
>Mature Secondary Structure
MNHHVVFLERDSLKVEVRRPNFEHTYEEYAKTKPDQVVSHLQHATIAIINKVGLSEETLA
CCCEEEEEECCCEEEEEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
QLPKLKMVAIAATGYDAVDIGACRRHGIAVANIRNYAIHTVPEHVFALVLALRRNLLAYR
HCCCEEEEEEEECCCCCEEHHHHHHCCEEEEEECCCEEHHHHHHHHHHHHHHHHHHHHHH
QEVEQGRWQKSEQFCLFTHEIRELFGATIGIVGEGTIGRGTAAIAQGFGMRVLFAHHESR
HHHHHHCCCCCCCEEEHHHHHHHHHCCEEEEEECCCCCCCHHHHHHCCCEEEEEEECCCC
KKKDMVLTPFDQVLAESDILSLHCPLTPATRNLIGVNELRKMKRSALLINTARGGLVDEA
CCCCCEECCHHHHHCCCCEEEEECCCCHHHHCCCCHHHHHHHHHHHEEEECCCCCCCHHH
ALVQALDDGTIAGAGFDVLTTEPPRNGNPLLGLRRPNFILTPHIAWASTEAMRFLADQLV
HHHHHHCCCEEECCCCEEEECCCCCCCCCEEEECCCCEEEECCHHHHHHHHHHHHHHHHH
DNIEAWVAGKPQNLLT
HHHHHHHCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144463; 1729225; 1657886 [H]