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The map label for this gene is ispB [H]

Identifier: 82702937

GI number: 82702937

Start: 2074994

End: 2075962

Strand: Reverse

Name: ispB [H]

Synonym: Nmul_A1814

Alternate gene names: 82702937

Gene position: 2075962-2074994 (Counterclockwise)

Preceding gene: 82702945

Following gene: 82702936

Centisome position: 65.19

GC content: 55.62

Gene sequence:

>969_bases
GTGTCAATTGAACGTATCAGAAGCCTGATTGCCCCCGATATGGGCAGGGTGGATACCGTAATTCGTGAAAAACTCCATTC
GGAGGTCGTCCTCATCCGGCAGGTAAGCGAATATATCATCAACAGCGGCGGGAAGCGTCTGCGGCCCGCTTTGGTAATCC
TGTCCGCTGGCGCATTCGGCTATACCGGACAATACCATTACAATCTTGCTGCCATCGTGGAATTCATCCATACGGCAACC
CTTCTGCATGACGACGTTGTGGACGCTTCCGAACTGAGGCGAAGCAAGGCGACTGCAAATGCCCTGTTTGGAAACGCCGC
CAGCGTATTGGTCGGGGATTTTCTATATTCCCGGGCATTCCAGATGATGGTCGAAGTGGACAATATGCGGGTCATGCGGG
TGTTGGCCGATGCGACCAACACGATTGCCGAAGGCGAAGTGTTGCAACTGCTCAATGTCCGTGATCCGGACGTCGATGAG
GAAAATTACCTGCGTGTGATCCGCTATAAGACCGCGAAGCTGTTCGAAGCCGCAACGCGGCTCGGCGCGATCCTCGGTAA
TGCCTCCCCAGCGGAGGAAGAAGCGATGGCCGTGTATGGCATGCATCTCGGCACCGCCTTCCAACTTACCGATGACATGC
TGGATTATTCCGGGGATTATCAGGAAACCGGCAAGAATCTGGGCGACGATCTGGCAGAAGGCAAACCCACTCTGCCGTTG
ATCTATGCCATGAAAACAGGATCGGGCGCCCAGGCGCAGATCATCCGCAGCGCCATCGAGCGGGGAGGCGAGGGGGGATT
TCAGCCCGTGCTGGAAGTTATCCAGCAGACCGGCGCATTGGATTATGCCAGACAACGCGCGCAGGCCGAATCCGAGACCG
CGTCCAACATGATTTCCTCACTGCCGGATTCAGACTATAAGCAATGCCTGCTGGATCTGAGCACCTTTGCGGTGGCGAGA
AATCATTAA

Upstream 100 bases:

>100_bases
GCGGATGAGTTTTGTGTCCCGATCCGAGTTTTGCCTGTTTTCGAACTTGACACGTTGGCGGCGACATTTCTACCATGGCA
TCTTTTTTGCAGGAACCTCC

Downstream 100 bases:

>100_bases
TGGTTTTATCTGGACCTGATTCACCTGCTTTAGCGCAGGGGCGCGGTGACGCAGTCACCGCGCTAATTTGGATTGCTCCA
AAACCCCTTCCTGCGTACAA

Product: farnesyltranstransferase

Products: NA

Alternate protein names: All-trans-octaprenyl-diphosphate synthase; Octaprenyl pyrophosphate synthase; OPP synthase [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSIERIRSLIAPDMGRVDTVIREKLHSEVVLIRQVSEYIINSGGKRLRPALVILSAGAFGYTGQYHYNLAAIVEFIHTAT
LLHDDVVDASELRRSKATANALFGNAASVLVGDFLYSRAFQMMVEVDNMRVMRVLADATNTIAEGEVLQLLNVRDPDVDE
ENYLRVIRYKTAKLFEAATRLGAILGNASPAEEEAMAVYGMHLGTAFQLTDDMLDYSGDYQETGKNLGDDLAEGKPTLPL
IYAMKTGSGAQAQIIRSAIERGGEGGFQPVLEVIQQTGALDYARQRAQAESETASNMISSLPDSDYKQCLLDLSTFAVAR
NH

Sequences:

>Translated_322_residues
MSIERIRSLIAPDMGRVDTVIREKLHSEVVLIRQVSEYIINSGGKRLRPALVILSAGAFGYTGQYHYNLAAIVEFIHTAT
LLHDDVVDASELRRSKATANALFGNAASVLVGDFLYSRAFQMMVEVDNMRVMRVLADATNTIAEGEVLQLLNVRDPDVDE
ENYLRVIRYKTAKLFEAATRLGAILGNASPAEEEAMAVYGMHLGTAFQLTDDMLDYSGDYQETGKNLGDDLAEGKPTLPL
IYAMKTGSGAQAQIIRSAIERGGEGGFQPVLEVIQQTGALDYARQRAQAESETASNMISSLPDSDYKQCLLDLSTFAVAR
NH
>Mature_321_residues
SIERIRSLIAPDMGRVDTVIREKLHSEVVLIRQVSEYIINSGGKRLRPALVILSAGAFGYTGQYHYNLAAIVEFIHTATL
LHDDVVDASELRRSKATANALFGNAASVLVGDFLYSRAFQMMVEVDNMRVMRVLADATNTIAEGEVLQLLNVRDPDVDEE
NYLRVIRYKTAKLFEAATRLGAILGNASPAEEEAMAVYGMHLGTAFQLTDDMLDYSGDYQETGKNLGDDLAEGKPTLPLI
YAMKTGSGAQAQIIRSAIERGGEGGFQPVLEVIQQTGALDYARQRAQAESETASNMISSLPDSDYKQCLLDLSTFAVARN
H

Specific function: Supplies octaprenyl diphosphate, the precursor for the side chain of the isoprenoid quinones ubiquinone and menaquinone [H]

COG id: COG0142

COG function: function code H; Geranylgeranyl pyrophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FPP/GGPP synthase family [H]

Homologues:

Organism=Homo sapiens, GI50659086, Length=315, Percent_Identity=31.7460317460317, Blast_Score=133, Evalue=2e-31,
Organism=Escherichia coli, GI1789578, Length=322, Percent_Identity=54.9689440993789, Blast_Score=372, Evalue=1e-104,
Organism=Escherichia coli, GI1786623, Length=218, Percent_Identity=35.7798165137615, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17505681, Length=300, Percent_Identity=30.6666666666667, Blast_Score=143, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6319475, Length=297, Percent_Identity=31.3131313131313, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6325188, Length=274, Percent_Identity=25.1824817518248, Blast_Score=80, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24651612, Length=294, Percent_Identity=28.2312925170068, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI281365769, Length=280, Percent_Identity=24.2857142857143, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24660002, Length=280, Percent_Identity=24.2857142857143, Blast_Score=76, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000092
- InterPro:   IPR017446
- InterPro:   IPR008949 [H]

Pfam domain/function: PF00348 polyprenyl_synt [H]

EC number: =2.5.1.90 [H]

Molecular weight: Translated: 35272; Mature: 35141

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00444 POLYPRENYL_SYNTHET_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIERIRSLIAPDMGRVDTVIREKLHSEVVLIRQVSEYIINSGGKRLRPALVILSAGAFG
CCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEEEECCCCC
YTGQYHYNLAAIVEFIHTATLLHDDVVDASELRRSKATANALFGNAASVLVGDFLYSRAF
CCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QMMVEVDNMRVMRVLADATNTIAEGEVLQLLNVRDPDVDEENYLRVIRYKTAKLFEAATR
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LGAILGNASPAEEEAMAVYGMHLGTAFQLTDDMLDYSGDYQETGKNLGDDLAEGKPTLPL
HHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEE
IYAMKTGSGAQAQIIRSAIERGGEGGFQPVLEVIQQTGALDYARQRAQAESETASNMISS
EEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LPDSDYKQCLLDLSTFAVARNH
CCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIERIRSLIAPDMGRVDTVIREKLHSEVVLIRQVSEYIINSGGKRLRPALVILSAGAFG
CHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEEEEECCCCC
YTGQYHYNLAAIVEFIHTATLLHDDVVDASELRRSKATANALFGNAASVLVGDFLYSRAF
CCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
QMMVEVDNMRVMRVLADATNTIAEGEVLQLLNVRDPDVDEENYLRVIRYKTAKLFEAATR
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
LGAILGNASPAEEEAMAVYGMHLGTAFQLTDDMLDYSGDYQETGKNLGDDLAEGKPTLPL
HHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCEE
IYAMKTGSGAQAQIIRSAIERGGEGGFQPVLEVIQQTGALDYARQRAQAESETASNMISS
EEEEECCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LPDSDYKQCLLDLSTFAVARNH
CCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8312607; 9278503; 2670911; 8037730 [H]