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The map label for this gene is rpoE [H]

Identifier: 82702869

GI number: 82702869

Start: 1999395

End: 1999997

Strand: Direct

Name: rpoE [H]

Synonym: Nmul_A1746

Alternate gene names: 82702869

Gene position: 1999395-1999997 (Clockwise)

Preceding gene: 82702868

Following gene: 82702870

Centisome position: 62.79

GC content: 52.57

Gene sequence:

>603_bases
ATGGGTGACCGGGAAATCGATCAGCAACTGGTGGAACGTGCGCAACACGGGGACAAGCAGGCCTTCGATCTTCTGGTGAT
CAAGTACCAGCGCAAGCTTGCGCGTCTCCTTTCGCAATTCATCAGGGATCCGTCGGAAGTAGAGGATGTGACTCAGGAGG
CCTTCATCAAGGCTTACCGTGCGCTACCGTCGTTCCGTGGAGACAGTGCATTTTATACCTGGTTGTACAGGATAGGCATC
AATACTGCCAAGAATTTTCTGGTTGCTCAAGGACGGCGGGCGCCTACCACTACTACAGGATTCGACAGCGAAGAGGCCGA
AGGTTTTGAAGAAGCGAGCCAGCTTCGCGAAATGAACACGCCCGAGAGCGAACTGACAAGCAAGCAGATCGCTCAAACGG
TGAACCAAACGCTGGAAGAATTGCCTGAAGAACTGCGAACGGCAATTACGTTGAGAGAAATTGAGGGACTGAGCTACGAG
GAAATCGCAGCGATCATGAATTGTCCTATCGGCACGGTCCGTTCGCGAATATTTCGTGCACGGGAAGCAATAGCGGAAAA
ACTGCGCCCCTTGCTGGGGACTGGTAAAGATAAGAGGTGGTAA

Upstream 100 bases:

>100_bases
CGTAATTCCCGATGGGCTCCTGAACGGGTATACTTGGCTGAGCTGCCTTATAACGAAGCAGTTACAAGTAAGAGATTCGT
CAATTCAGGAATTAGGTGGC

Downstream 100 bases:

>100_bases
TATGCAAGAAAAAATATCGGCATTGATGGATGGTGAACTGGACGCGGGCGATGCCTCGGCAATGATCGAGCAATTCAAGA
ACGCGGAGGAATTACGGGAA

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MGDREIDQQLVERAQHGDKQAFDLLVIKYQRKLARLLSQFIRDPSEVEDVTQEAFIKAYRALPSFRGDSAFYTWLYRIGI
NTAKNFLVAQGRRAPTTTTGFDSEEAEGFEEASQLREMNTPESELTSKQIAQTVNQTLEELPEELRTAITLREIEGLSYE
EIAAIMNCPIGTVRSRIFRAREAIAEKLRPLLGTGKDKRW

Sequences:

>Translated_200_residues
MGDREIDQQLVERAQHGDKQAFDLLVIKYQRKLARLLSQFIRDPSEVEDVTQEAFIKAYRALPSFRGDSAFYTWLYRIGI
NTAKNFLVAQGRRAPTTTTGFDSEEAEGFEEASQLREMNTPESELTSKQIAQTVNQTLEELPEELRTAITLREIEGLSYE
EIAAIMNCPIGTVRSRIFRAREAIAEKLRPLLGTGKDKRW
>Mature_199_residues
GDREIDQQLVERAQHGDKQAFDLLVIKYQRKLARLLSQFIRDPSEVEDVTQEAFIKAYRALPSFRGDSAFYTWLYRIGIN
TAKNFLVAQGRRAPTTTTGFDSEEAEGFEEASQLREMNTPESELTSKQIAQTVNQTLEELPEELRTAITLREIEGLSYEE
IAAIMNCPIGTVRSRIFRAREAIAEKLRPLLGTGKDKRW

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=193, Percent_Identity=62.6943005181347, Blast_Score=247, Evalue=4e-67,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22873; Mature: 22742

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDREIDQQLVERAQHGDKQAFDLLVIKYQRKLARLLSQFIRDPSEVEDVTQEAFIKAYR
CCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
ALPSFRGDSAFYTWLYRIGINTAKNFLVAQGRRAPTTTTGFDSEEAEGFEEASQLREMNT
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
PESELTSKQIAQTVNQTLEELPEELRTAITLREIEGLSYEEIAAIMNCPIGTVRSRIFRA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHH
REAIAEKLRPLLGTGKDKRW
HHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GDREIDQQLVERAQHGDKQAFDLLVIKYQRKLARLLSQFIRDPSEVEDVTQEAFIKAYR
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
ALPSFRGDSAFYTWLYRIGINTAKNFLVAQGRRAPTTTTGFDSEEAEGFEEASQLREMNT
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
PESELTSKQIAQTVNQTLEELPEELRTAITLREIEGLSYEEIAAIMNCPIGTVRSRIFRA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHH
REAIAEKLRPLLGTGKDKRW
HHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]