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The map label for this gene is treZ [H]

Identifier: 82702532

GI number: 82702532

Start: 1605003

End: 1606880

Strand: Reverse

Name: treZ [H]

Synonym: Nmul_A1403

Alternate gene names: 82702532

Gene position: 1606880-1605003 (Counterclockwise)

Preceding gene: 82702533

Following gene: 82702531

Centisome position: 50.46

GC content: 55.75

Gene sequence:

>1878_bases
GTGCTGACACGACTTCACGACATGCCTTATGGCACTCAAATCACGGACCACGGAGTGCGCTTCCGTCTTTGGGCGCCTGG
TTGCAAACAGGTGGGATTGTGCCTTGCAGACGGACGGACGAATGAGAAGCCCGAACGGGCACTCCCCATGACTCCCCGCG
CAGGAGGATGGTTCGAGTCGACTGTAGCGGAAGCGGGAGCGGGAATACGCTATGGGTTCGATGTGAACGGTGCCCGCGTG
CCCGATCCCGCTTCGCGCTTCAATCCGGATGATGTGCATGGTTTAAGTGAGGTGATCGATCCTTCGGCCTTCGCCTGGCA
GGATGCGAAATGGCGTGGACGGCCATGGGAAGAAGCCGTCATCTATGAGCTGCATGTAGGCACGTTCTCGCCCGAAGGAA
CCTTCAAGGGCGTCTCCCAGAGACTGGACTATCTGGTCGAACTCGGTGTGACCGCCATTGAGCTCATGCCGGTTGCCGAT
TTTCCGGGCTCACGAAACTGGGGCTATGACGGCGTTCTGCTTTACGCGCCGGATAGCCGTTACGGACGTCCGGAGGATCT
CAAGGAACTGGTTCAGGCCGCGCATGAACGGGGACTGATGATTCTGCTCGATGTCGTCTACAATCACTTCGGGCCGGAAG
GAAACTATCTTCACCTTTATGCAAAGGAATTCTTTACTGGGCGCCATCACACTCCGTGGGGTGCGGCAATAAATTTCGAT
GGCCCCGCAAGTGGACGGGTACGCGAGTTTTTCATCCACAACGCGTTGTATTGGCTGCAAGAGTATCATTTCGACGGCCT
GCGGCTCGATGCAGTCCACGCGATCATCGACCATTCCAGTCCGCACATCCTTGTCGAACTGGCGGAGCGTGTTCGTGCCG
AGGTCGGTACAGAGCGCCACGTGCATCTGATCCTGGAAAACGATGCCAACAATGCCCGCTATCTGACAAATAGTTGGTAC
GACGCGCAGTGGAACGACGATATTCACCACGCTCTGCATGTGCTTTCTACACAGGAGGGCGATGGATACTATGTGGATTA
TGCCGACAATCCGGTACGCCATCTCGGGCGCTGCCTGGCGGAAGGGTTTGCCTATCAGGGAGAGATATCCGTTTATCGCG
ACGATATGGCGCGTGGAGAGGCGAGCATTCATCTGCCGCCCCAGGCTTTCATTTCCTTTCTGCAATCCCACGACCAGGCG
GGCAACCGTGCATTTGGCGAGCGTATTAGTCATATTGGCGAGGAAGCGTTGACACGTATGGCGGCAGCCATCTATTTGCT
GGCTCCCGGCATTCCCATGCTGTTCATGGGCGAGGAGTTTGCCGCAAAATCGCCTTTCCTGTTTTTCTGCGACTTCGGAC
CGGAGCTGCGCGAAGCTGTGACACAGGGGCGACGCAGGGAATTTGCCCGCTTTGCGCATTTTACGCAAGACATGGACGAG
ACAGCGATACCGGACCCGAATGCTGTTCAGACTTTTCTTATCTCGAAAATCGATTGGGATTTACTCAGGAATGAAGCTCA
CTTTGCCTGGCTGGAGTATTACCGCAACCTTATGAAACTTCGCAGCGAAATTATCGTACCGCGCCTTCGCGGAATGAAGG
GTAATTCGGCACATTTTGAGGTATTCGCGCCTAAATGCCTGTGGGTCTGCTGGCAGTTGGGCGACGATTCCACCTTGCGG
CTATTGGCGAATTTTTCCGACGAGAGCGTGGCGGCTCCCCGGTTCGGCGGGCAGATCGTGTTTGCCTCACCCGGTGCAAT
ACCCCCATCTTCCCGAACAACCAAAAGTATCCCGGTGAAAAGTATGCTGGCGCCTCGTTCGGTAGTCTGGATGCTTGAAC
CGGCTGCCGATAAAAGTAAAGGCAGTTTTACCGGATAA

Upstream 100 bases:

>100_bases
AACGCGCTCGCGCAACAATCGAAGGCAGTCGATGGAACCATGCAGCGACTGATACGGCTGATGCAACACAGAAGGTAGAA
CAAAGGGAAGAGGGAAAGGC

Downstream 100 bases:

>100_bases
CAACCGCCGAAACTGAAATGAAGGGAAAGCACAAGGAAATATTCGAGGGAGAAGCATGAGCAATCCGCTTGACCAGCTGT
GCGAATTATATGGCGTGCTG

Product: alpha amylase catalytic subunit

Products: NA

Alternate protein names: MTHase; 4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase; Maltooligosyl trehalose trehalohydrolase [H]

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MLTRLHDMPYGTQITDHGVRFRLWAPGCKQVGLCLADGRTNEKPERALPMTPRAGGWFESTVAEAGAGIRYGFDVNGARV
PDPASRFNPDDVHGLSEVIDPSAFAWQDAKWRGRPWEEAVIYELHVGTFSPEGTFKGVSQRLDYLVELGVTAIELMPVAD
FPGSRNWGYDGVLLYAPDSRYGRPEDLKELVQAAHERGLMILLDVVYNHFGPEGNYLHLYAKEFFTGRHHTPWGAAINFD
GPASGRVREFFIHNALYWLQEYHFDGLRLDAVHAIIDHSSPHILVELAERVRAEVGTERHVHLILENDANNARYLTNSWY
DAQWNDDIHHALHVLSTQEGDGYYVDYADNPVRHLGRCLAEGFAYQGEISVYRDDMARGEASIHLPPQAFISFLQSHDQA
GNRAFGERISHIGEEALTRMAAAIYLLAPGIPMLFMGEEFAAKSPFLFFCDFGPELREAVTQGRRREFARFAHFTQDMDE
TAIPDPNAVQTFLISKIDWDLLRNEAHFAWLEYYRNLMKLRSEIIVPRLRGMKGNSAHFEVFAPKCLWVCWQLGDDSTLR
LLANFSDESVAAPRFGGQIVFASPGAIPPSSRTTKSIPVKSMLAPRSVVWMLEPAADKSKGSFTG

Sequences:

>Translated_625_residues
MLTRLHDMPYGTQITDHGVRFRLWAPGCKQVGLCLADGRTNEKPERALPMTPRAGGWFESTVAEAGAGIRYGFDVNGARV
PDPASRFNPDDVHGLSEVIDPSAFAWQDAKWRGRPWEEAVIYELHVGTFSPEGTFKGVSQRLDYLVELGVTAIELMPVAD
FPGSRNWGYDGVLLYAPDSRYGRPEDLKELVQAAHERGLMILLDVVYNHFGPEGNYLHLYAKEFFTGRHHTPWGAAINFD
GPASGRVREFFIHNALYWLQEYHFDGLRLDAVHAIIDHSSPHILVELAERVRAEVGTERHVHLILENDANNARYLTNSWY
DAQWNDDIHHALHVLSTQEGDGYYVDYADNPVRHLGRCLAEGFAYQGEISVYRDDMARGEASIHLPPQAFISFLQSHDQA
GNRAFGERISHIGEEALTRMAAAIYLLAPGIPMLFMGEEFAAKSPFLFFCDFGPELREAVTQGRRREFARFAHFTQDMDE
TAIPDPNAVQTFLISKIDWDLLRNEAHFAWLEYYRNLMKLRSEIIVPRLRGMKGNSAHFEVFAPKCLWVCWQLGDDSTLR
LLANFSDESVAAPRFGGQIVFASPGAIPPSSRTTKSIPVKSMLAPRSVVWMLEPAADKSKGSFTG
>Mature_625_residues
MLTRLHDMPYGTQITDHGVRFRLWAPGCKQVGLCLADGRTNEKPERALPMTPRAGGWFESTVAEAGAGIRYGFDVNGARV
PDPASRFNPDDVHGLSEVIDPSAFAWQDAKWRGRPWEEAVIYELHVGTFSPEGTFKGVSQRLDYLVELGVTAIELMPVAD
FPGSRNWGYDGVLLYAPDSRYGRPEDLKELVQAAHERGLMILLDVVYNHFGPEGNYLHLYAKEFFTGRHHTPWGAAINFD
GPASGRVREFFIHNALYWLQEYHFDGLRLDAVHAIIDHSSPHILVELAERVRAEVGTERHVHLILENDANNARYLTNSWY
DAQWNDDIHHALHVLSTQEGDGYYVDYADNPVRHLGRCLAEGFAYQGEISVYRDDMARGEASIHLPPQAFISFLQSHDQA
GNRAFGERISHIGEEALTRMAAAIYLLAPGIPMLFMGEEFAAKSPFLFFCDFGPELREAVTQGRRREFARFAHFTQDMDE
TAIPDPNAVQTFLISKIDWDLLRNEAHFAWLEYYRNLMKLRSEIIVPRLRGMKGNSAHFEVFAPKCLWVCWQLGDDSTLR
LLANFSDESVAAPRFGGQIVFASPGAIPPSSRTTKSIPVKSMLAPRSVVWMLEPAADKSKGSFTG

Specific function: Hydrolyzes The Alpha-1,6-Glucosidic Linkages In Glycogen Which Has First Been Partially Depolymerized By Phosphorylase. Shows Only Very Little Activity With Native Glycogen. [C]

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Homo sapiens, GI189458812, Length=167, Percent_Identity=34.1317365269461, Blast_Score=87, Evalue=6e-17,
Organism=Escherichia coli, GI2367229, Length=337, Percent_Identity=26.1127596439169, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1789839, Length=282, Percent_Identity=28.3687943262411, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1790687, Length=99, Percent_Identity=43.4343434343434, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17554896, Length=175, Percent_Identity=35.4285714285714, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI32564391, Length=175, Percent_Identity=35.4285714285714, Blast_Score=90, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6320826, Length=209, Percent_Identity=32.5358851674641, Blast_Score=84, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6322245, Length=103, Percent_Identity=34.9514563106796, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI28573410, Length=176, Percent_Identity=31.25, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- InterPro:   IPR015156
- InterPro:   IPR012768 [H]

Pfam domain/function: PF09071 Alpha-amyl_C; PF00128 Alpha-amylase; PF02922 CBM_48 [H]

EC number: =3.2.1.141 [H]

Molecular weight: Translated: 70397; Mature: 70397

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRLHDMPYGTQITDHGVRFRLWAPGCKQVGLCLADGRTNEKPERALPMTPRAGGWFES
CCCCCCCCCCCCEEECCCEEEEEECCCCHHCCEEEECCCCCCCCCCCCCCCCCCCCCHHH
TVAEAGAGIRYGFDVNGARVPDPASRFNPDDVHGLSEVIDPSAFAWQDAKWRGRPWEEAV
HHHHCCCCEEEEECCCCCCCCCCHHCCCCHHHHHHHHHCCCCHHEECCCCCCCCCCCCEE
IYELHVGTFSPEGTFKGVSQRLDYLVELGVTAIELMPVADFPGSRNWGYDGVLLYAPDSR
EEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCEEEEECCCCC
YGRPEDLKELVQAAHERGLMILLDVVYNHFGPEGNYLHLYAKEFFTGRHHTPWGAAINFD
CCCHHHHHHHHHHHHHCCEEEEEHHHHHHHCCCCCEEEEEEHHHHCCCCCCCCCCEEECC
GPASGRVREFFIHNALYWLQEYHFDGLRLDAVHAIIDHSSPHILVELAERVRAEVGTERH
CCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCE
VHLILENDANNARYLTNSWYDAQWNDDIHHALHVLSTQEGDGYYVDYADNPVRHLGRCLA
EEEEEECCCCCCEEEECCEECCCCCCHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHH
EGFAYQGEISVYRDDMARGEASIHLPPQAFISFLQSHDQAGNRAFGERISHIGEEALTRM
HCCCCCCEEEEEHHHHHCCCCEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
AAAIYLLAPGIPMLFMGEEFAAKSPFLFFCDFGPELREAVTQGRRREFARFAHFTQDMDE
HHHHHHHCCCCCEEEECCHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHH
TAIPDPNAVQTFLISKIDWDLLRNEAHFAWLEYYRNLMKLRSEIIVPRLRGMKGNSAHFE
HCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
VFAPKCLWVCWQLGDDSTLRLLANFSDESVAAPRFGGQIVFASPGAIPPSSRTTKSIPVK
EECCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHH
SMLAPRSVVWMLEPAADKSKGSFTG
HHCCCCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MLTRLHDMPYGTQITDHGVRFRLWAPGCKQVGLCLADGRTNEKPERALPMTPRAGGWFES
CCCCCCCCCCCCEEECCCEEEEEECCCCHHCCEEEECCCCCCCCCCCCCCCCCCCCCHHH
TVAEAGAGIRYGFDVNGARVPDPASRFNPDDVHGLSEVIDPSAFAWQDAKWRGRPWEEAV
HHHHCCCCEEEEECCCCCCCCCCHHCCCCHHHHHHHHHCCCCHHEECCCCCCCCCCCCEE
IYELHVGTFSPEGTFKGVSQRLDYLVELGVTAIELMPVADFPGSRNWGYDGVLLYAPDSR
EEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCEEEEECCCCC
YGRPEDLKELVQAAHERGLMILLDVVYNHFGPEGNYLHLYAKEFFTGRHHTPWGAAINFD
CCCHHHHHHHHHHHHHCCEEEEEHHHHHHHCCCCCEEEEEEHHHHCCCCCCCCCCEEECC
GPASGRVREFFIHNALYWLQEYHFDGLRLDAVHAIIDHSSPHILVELAERVRAEVGTERH
CCCCCCHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCE
VHLILENDANNARYLTNSWYDAQWNDDIHHALHVLSTQEGDGYYVDYADNPVRHLGRCLA
EEEEEECCCCCCEEEECCEECCCCCCHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHH
EGFAYQGEISVYRDDMARGEASIHLPPQAFISFLQSHDQAGNRAFGERISHIGEEALTRM
HCCCCCCEEEEEHHHHHCCCCEEECCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
AAAIYLLAPGIPMLFMGEEFAAKSPFLFFCDFGPELREAVTQGRRREFARFAHFTQDMDE
HHHHHHHCCCCCEEEECCHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCHH
TAIPDPNAVQTFLISKIDWDLLRNEAHFAWLEYYRNLMKLRSEIIVPRLRGMKGNSAHFE
HCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
VFAPKCLWVCWQLGDDSTLRLLANFSDESVAAPRFGGQIVFASPGAIPPSSRTTKSIPVK
EECCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCHH
SMLAPRSVVWMLEPAADKSKGSFTG
HHCCCCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8899719; 11427726 [H]