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The map label for this gene is uvrB [H]

Identifier: 82702490

GI number: 82702490

Start: 1548172

End: 1550259

Strand: Direct

Name: uvrB [H]

Synonym: Nmul_A1361

Alternate gene names: 82702490

Gene position: 1548172-1550259 (Clockwise)

Preceding gene: 82702488

Following gene: 82702494

Centisome position: 48.62

GC content: 53.59

Gene sequence:

>2088_bases
GTGATTATTACCTTTCCCAATAGCCCTTATAAGCTCAACCAGTTATTTCAGCCCGCAGGCGATCAGTCTTCAGCGATCGA
AAAACTGGTGGAGGGAATAGAGGACGGGCTGGCATTCCAGACACTGCTGGGAGTGACGGGCTCCGGCAAGACCTTTACGA
TGGCCAATGTCATTGCGCGGCTCGGACGTCCCGCGATTATCATGGCGCCAAACAAGACCCTCGCGGCGCAGTTGTATGCG
GAAATGCGGGAGTTTTTCCCGGAAAATGCCGTCGAGTACTTTGTATCGTATTACGACTATTATCAGCCGGAAGCATATGT
CCCGTCACGGGACCTTTTTATCGAGAAAGATTCCGCAATCAACGAGCATATCGAGCAGATGCGCCTGTCTGCCACCAAGG
CACTGCTCGAGCGGGACGATGCCATCATCGTCGCCACGGTGTCGTGCATTTATGGAATAGGCGATCCCGTGGACTATCAC
GGAATGATCCTCCATCTGCGGGAGCATGAAAAGATCACCCAAAGGCAGGCAATACAGCGTCTGACAGAAATGCAATACGA
GCGTAACGAATTCGAGTTCAGCCGAGGAACCTTCCGGGTGAGGGGAGATGTGCTCGACATCTTTCCCGCGGAAAGCTCGG
AAACCGCCGTGCGTGTTTCCCTGTTTGATGACGAGGTGGAAAGCATGACATTGTTCGATCCTCTTACCGGTCGCACCTCG
AGAAAGGTATCGCGCTATACCGTTTATCCTTCCAGCCATTACGTGACGCCAAGGAGCACCACTTTGCGCGCCATCGAGAC
AATCAAGACCGAGTTGAGGGAACGCATGGATTTTTTCCACCAGCACAACAAGCTGGTAGAGTTGCAACGCATCGAACAAC
GCACTCGTTTCGATCTGGAAATGCTCAACGAACTGGGTTTCTGCAAAGGCATCGAGAACTATTCGCGTCATTTGTCAGGA
AAAAAACCGGGAGAACCGCCGCCCACCTTGATGGATTACCTGCCGCCCCGAACGCTGATGGTGATCGATGAGAGTCACGT
AACGGTTCCGCAAGTCGGAGGAATGTACAAGGGTGATCGGGCCCGCAAGGAAAATCTTGTCGATTATGGGTTTCGCCTGC
CTTCAGCGCTGGATAACCGTCCCCTCCGGTTCGATGAATTCAGGAAAATCATGCCGCAAACGGTGTTTGTGTCTGCGACA
CCGGCGGAATTCGAAAAGATACACAGTGGTCAGGTTGTCGAGCAGGTGGTGCGGCCCACAGGTCTCGTAGATCCGCAGAT
CGAAGTCAGACCGGCGAGCACTCAGGTGGACGATCTGATGTCGGAAGTAAGGCTGCGTACCGCAAAGGATGAGCGGGTGC
TGGTAACGACGCTCACCAAACGCATGGCGGAGGATCTGACGGATTATTTTGCCGACCACGGCATCAGAGTGCGGTATCTG
CATTCCGATATCGATACGGTGGAAAGGGTGGAGATTGTGCGTGACCTGAGACTGGGAAAATTCGATGTGCTGGTCGGCAT
CAATCTGTTGCGTGAGGGGCTGGATATGCCGGAGGTATCGCTGGTAGCCATACTGGATGCGGACAAGGAGGGCTTTCTTC
GCTCAGAGCGTTCATTGATACAGACCATCGGCCGCGCGGCGCGCCACATCAATGGCACTGCACTTCTTTATGCGGACAGG
ATTACAGAATCCATGCGGCTGGCAATGGATGAGACCGAGCGACGGCGCAACAAGCAGATCCTGTTCAATCAGGAGCATGG
GATTACGCCGCGAAGCGTGCATAAGCGCGTCAAGGACCTGATTGACGGTGTTTACAGCATGGAAACAGCCCAGCAGCAGC
TTAAGGCAGCGCAAACGCAGGCGCGGTACGAAGCCATGAGCGAGACGCAGCTCGCAAAAGAAATCAAGTCACTGGAAAAA
CAGATGCACGAGGCAGCAAAAAATCTCGAATTCGAGAAGGCTGCGGAGTATCGGGATGAATTAAAAAAATTAAAAAACAA
GCTCTTTGTCGGATTTGTGGAGCCCCAGGATGAGAAAGGAACGGGAGCGGCGAAATCCCGGGGGAAGGTGCCAAGAGCGG
TGAGATAG

Upstream 100 bases:

>100_bases
CTTCTCCAGTTTGACGAGGGGCCAGGCGAAGCAGAAACAATGCTGCAAGTCATGGAATTAACTGAAAAACAGTAGACAAC
GATGGATTTGGATTATACCC

Downstream 100 bases:

>100_bases
CGACGGATTAAAGCCGGGAAGCGTCAACATGCTTTTCTGCGCTAATTGATCACCGTGGCCGAGTTCGCAAAAATAATTTC
CTTGAAGGTACGATTATGAG

Product: excinuclease ABC subunit B

Products: NA

Alternate protein names: Protein uvrB; Excinuclease ABC subunit B [H]

Number of amino acids: Translated: 695; Mature: 695

Protein sequence:

>695_residues
MIITFPNSPYKLNQLFQPAGDQSSAIEKLVEGIEDGLAFQTLLGVTGSGKTFTMANVIARLGRPAIIMAPNKTLAAQLYA
EMREFFPENAVEYFVSYYDYYQPEAYVPSRDLFIEKDSAINEHIEQMRLSATKALLERDDAIIVATVSCIYGIGDPVDYH
GMILHLREHEKITQRQAIQRLTEMQYERNEFEFSRGTFRVRGDVLDIFPAESSETAVRVSLFDDEVESMTLFDPLTGRTS
RKVSRYTVYPSSHYVTPRSTTLRAIETIKTELRERMDFFHQHNKLVELQRIEQRTRFDLEMLNELGFCKGIENYSRHLSG
KKPGEPPPTLMDYLPPRTLMVIDESHVTVPQVGGMYKGDRARKENLVDYGFRLPSALDNRPLRFDEFRKIMPQTVFVSAT
PAEFEKIHSGQVVEQVVRPTGLVDPQIEVRPASTQVDDLMSEVRLRTAKDERVLVTTLTKRMAEDLTDYFADHGIRVRYL
HSDIDTVERVEIVRDLRLGKFDVLVGINLLREGLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARHINGTALLYADR
ITESMRLAMDETERRRNKQILFNQEHGITPRSVHKRVKDLIDGVYSMETAQQQLKAAQTQARYEAMSETQLAKEIKSLEK
QMHEAAKNLEFEKAAEYRDELKKLKNKLFVGFVEPQDEKGTGAAKSRGKVPRAVR

Sequences:

>Translated_695_residues
MIITFPNSPYKLNQLFQPAGDQSSAIEKLVEGIEDGLAFQTLLGVTGSGKTFTMANVIARLGRPAIIMAPNKTLAAQLYA
EMREFFPENAVEYFVSYYDYYQPEAYVPSRDLFIEKDSAINEHIEQMRLSATKALLERDDAIIVATVSCIYGIGDPVDYH
GMILHLREHEKITQRQAIQRLTEMQYERNEFEFSRGTFRVRGDVLDIFPAESSETAVRVSLFDDEVESMTLFDPLTGRTS
RKVSRYTVYPSSHYVTPRSTTLRAIETIKTELRERMDFFHQHNKLVELQRIEQRTRFDLEMLNELGFCKGIENYSRHLSG
KKPGEPPPTLMDYLPPRTLMVIDESHVTVPQVGGMYKGDRARKENLVDYGFRLPSALDNRPLRFDEFRKIMPQTVFVSAT
PAEFEKIHSGQVVEQVVRPTGLVDPQIEVRPASTQVDDLMSEVRLRTAKDERVLVTTLTKRMAEDLTDYFADHGIRVRYL
HSDIDTVERVEIVRDLRLGKFDVLVGINLLREGLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARHINGTALLYADR
ITESMRLAMDETERRRNKQILFNQEHGITPRSVHKRVKDLIDGVYSMETAQQQLKAAQTQARYEAMSETQLAKEIKSLEK
QMHEAAKNLEFEKAAEYRDELKKLKNKLFVGFVEPQDEKGTGAAKSRGKVPRAVR
>Mature_695_residues
MIITFPNSPYKLNQLFQPAGDQSSAIEKLVEGIEDGLAFQTLLGVTGSGKTFTMANVIARLGRPAIIMAPNKTLAAQLYA
EMREFFPENAVEYFVSYYDYYQPEAYVPSRDLFIEKDSAINEHIEQMRLSATKALLERDDAIIVATVSCIYGIGDPVDYH
GMILHLREHEKITQRQAIQRLTEMQYERNEFEFSRGTFRVRGDVLDIFPAESSETAVRVSLFDDEVESMTLFDPLTGRTS
RKVSRYTVYPSSHYVTPRSTTLRAIETIKTELRERMDFFHQHNKLVELQRIEQRTRFDLEMLNELGFCKGIENYSRHLSG
KKPGEPPPTLMDYLPPRTLMVIDESHVTVPQVGGMYKGDRARKENLVDYGFRLPSALDNRPLRFDEFRKIMPQTVFVSAT
PAEFEKIHSGQVVEQVVRPTGLVDPQIEVRPASTQVDDLMSEVRLRTAKDERVLVTTLTKRMAEDLTDYFADHGIRVRYL
HSDIDTVERVEIVRDLRLGKFDVLVGINLLREGLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARHINGTALLYADR
ITESMRLAMDETERRRNKQILFNQEHGITPRSVHKRVKDLIDGVYSMETAQQQLKAAQTQARYEAMSETQLAKEIKSLEK
QMHEAAKNLEFEKAAEYRDELKKLKNKLFVGFVEPQDEKGTGAAKSRGKVPRAVR

Specific function: The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 uvrA and 2 uvrB subunits scans DNA for abnormalities. Upon binding of the uvrA(2)B(2) complex to a putative damaged site, the DNA

COG id: COG0556

COG function: function code L; Helicase subunit of the DNA excision repair complex

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 UVR domain [H]

Homologues:

Organism=Escherichia coli, GI1786996, Length=678, Percent_Identity=65.0442477876106, Blast_Score=887, Evalue=0.0,
Organism=Escherichia coli, GI1787357, Length=307, Percent_Identity=23.1270358306189, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR006935
- InterPro:   IPR001943
- InterPro:   IPR004807
- InterPro:   IPR009055 [H]

Pfam domain/function: PF00271 Helicase_C; PF04851 ResIII; PF02151 UVR [H]

EC number: NA

Molecular weight: Translated: 79575; Mature: 79575

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS50151 UVR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIITFPNSPYKLNQLFQPAGDQSSAIEKLVEGIEDGLAFQTLLGVTGSGKTFTMANVIAR
CEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHH
LGRPAIIMAPNKTLAAQLYAEMREFFPENAVEYFVSYYDYYQPEAYVPSRDLFIEKDSAI
CCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHH
NEHIEQMRLSATKALLERDDAIIVATVSCIYGIGDPVDYHGMILHLREHEKITQRQAIQR
HHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHH
LTEMQYERNEFEFSRGTFRVRGDVLDIFPAESSETAVRVSLFDDEVESMTLFDPLTGRTS
HHHHHHHHCHHEECCCEEEEECCEEEECCCCCCCCEEEEEEECCCHHHEEEECCCCCCCC
RKVSRYTVYPSSHYVTPRSTTLRAIETIKTELRERMDFFHQHNKLVELQRIEQRTRFDLE
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLNELGFCKGIENYSRHLSGKKPGEPPPTLMDYLPPRTLMVIDESHVTVPQVGGMYKGDR
HHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCH
ARKENLVDYGFRLPSALDNRPLRFDEFRKIMPQTVFVSATPAEFEKIHSGQVVEQVVRPT
HHHHHHHHHCCCCCHHHCCCCCCHHHHHHHCCCEEEEECCHHHHHHHCCCHHHHHHHCCC
GLVDPQIEVRPASTQVDDLMSEVRLRTAKDERVLVTTLTKRMAEDLTDYFADHGIRVRYL
CCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEE
HSDIDTVERVEIVRDLRLGKFDVLVGINLLREGLDMPEVSLVAILDADKEGFLRSERSLI
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHH
QTIGRAARHINGTALLYADRITESMRLAMDETERRRNKQILFNQEHGITPRSVHKRVKDL
HHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHH
IDGVYSMETAQQQLKAAQTQARYEAMSETQLAKEIKSLEKQMHEAAKNLEFEKAAEYRDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LKKLKNKLFVGFVEPQDEKGTGAAKSRGKVPRAVR
HHHHHHHEEEEEECCCCCCCCCCCHHCCCCCCCCC
>Mature Secondary Structure
MIITFPNSPYKLNQLFQPAGDQSSAIEKLVEGIEDGLAFQTLLGVTGSGKTFTMANVIAR
CEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHH
LGRPAIIMAPNKTLAAQLYAEMREFFPENAVEYFVSYYDYYQPEAYVPSRDLFIEKDSAI
CCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCEEEECCHHH
NEHIEQMRLSATKALLERDDAIIVATVSCIYGIGDPVDYHGMILHLREHEKITQRQAIQR
HHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHH
LTEMQYERNEFEFSRGTFRVRGDVLDIFPAESSETAVRVSLFDDEVESMTLFDPLTGRTS
HHHHHHHHCHHEECCCEEEEECCEEEECCCCCCCCEEEEEEECCCHHHEEEECCCCCCCC
RKVSRYTVYPSSHYVTPRSTTLRAIETIKTELRERMDFFHQHNKLVELQRIEQRTRFDLE
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MLNELGFCKGIENYSRHLSGKKPGEPPPTLMDYLPPRTLMVIDESHVTVPQVGGMYKGDR
HHHHHHHHCCHHHHHHHCCCCCCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCH
ARKENLVDYGFRLPSALDNRPLRFDEFRKIMPQTVFVSATPAEFEKIHSGQVVEQVVRPT
HHHHHHHHHCCCCCHHHCCCCCCHHHHHHHCCCEEEEECCHHHHHHHCCCHHHHHHHCCC
GLVDPQIEVRPASTQVDDLMSEVRLRTAKDERVLVTTLTKRMAEDLTDYFADHGIRVRYL
CCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCEEEEE
HSDIDTVERVEIVRDLRLGKFDVLVGINLLREGLDMPEVSLVAILDADKEGFLRSERSLI
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHH
QTIGRAARHINGTALLYADRITESMRLAMDETERRRNKQILFNQEHGITPRSVHKRVKDL
HHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHH
IDGVYSMETAQQQLKAAQTQARYEAMSETQLAKEIKSLEKQMHEAAKNLEFEKAAEYRDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LKKLKNKLFVGFVEPQDEKGTGAAKSRGKVPRAVR
HHHHHHHEEEEEECCCCCCCCCCCHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]