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The map label for this gene is fliF [H]

Identifier: 82702474

GI number: 82702474

Start: 1533449

End: 1535152

Strand: Direct

Name: fliF [H]

Synonym: Nmul_A1345

Alternate gene names: 82702474

Gene position: 1533449-1535152 (Clockwise)

Preceding gene: 82702472

Following gene: 82702475

Centisome position: 48.16

GC content: 56.22

Gene sequence:

>1704_bases
GTGACAGCGGGTGCGAATGCCACAACGATGAACAGCGCAATGAACACCGCCGCAAACAACGCGGCCAAGGGCGCGGTAAG
CAGTATTCAAGGGGGACTGCAAAGCTTTCTGACGCGATTATCGAGCCAGCAGAAAATCGGTCTCATGCTGACCGTTTCTT
CCTTGGTTGCGTTGCTGGCCGCCGGATGGATGTGGACGCAGGGACCGGACTACAAGGTGCTGTTCAGCAATGTATCCGAC
GGTGACGGCGGCGCAATCATCGCGTCCCTCCAGCAAATGAATGTGCCCTACAAATTTGCCGAAGGCGGCGGAGCGATACT
CGTCCCATCGAGCCAGGTGCATGATACTCGCCTCAAACTTGCATCTCAGGGGTTGCCAAGGGGAGGTCTGGTAGGTTTCG
AACTGATGGAAAACCAGAAGTTCGGTACCAGCCAGTTTCTTGAACAGGTCAATTTCCAGCGGGCGCTGGAAGGTGAACTG
GCCCGTTCTGTCCAGGCATTGGCGGCTGTCGACAGCGCCAGGGTCCATCTGGCGATCCCGAAGCAATCGGTATTTATGCG
GGAGCAACAGAAACCGAGCGCATCCGTGCTGGTGAATCTCTACCCCGGCCGCACCCTCGATCCCCAGCAGGTCAATGCGA
TCGTCCACCTCGTTTCCAGCAGTGTGCCCAATCTGCATGTGAAGAATGTGACGGTAGTGGATCAACAAGGCGATCTACTG
ACCAGGATCGATGAATCGAACCCCAATACGGGCCTGGATCCAGCTCAGCTCAAGTACTTGCATGCGCTGGAGCAAAGCTA
TATCAAGCGGATCGAGGCGATTCTGATTCCCATCGTGGGACAGGAAAATGTTCGTGCTCAGGTCGCGGCCGAGGTCGATT
TTGCACACTCCGAACAAACCAGCGAAACATACAGACCCAACCAGGATCCTGCAGGCTCCACCATCCGCAGTCAGCAGAGT
TCGGAATCCACGAATGTGGGCACACAGAATGAAGGCGGCGTTCCGGGCGCGCTTTCCAATCAGCCTCCGGTTCCCGCAAC
CGCTCCGGTCACCACGCCGCCAGCTCCTCCCGGAGCAACTGCCTCCCCATCCCGCGCCCCAAGCAGCACGCGCAAGGATG
CAACCATCAATTACGAAGTCGATAAAACAATCCAGCATGTGCGCAAACCAGTCGGTGGTATAAAAAGACTTTCTGTTGCA
GTCGTGGTCAACCACCGCAGAGTCGTGGACGAGAATGGTGTTGTCAGCGCCAAGCCACTGAGCGAGGTGGAAAAATCCCA
GATTACCGATCTGGTGAAAGAGGCGGTCGGATTCAGCAGGGAACGTGGGGATACACTGAACGTGGTTAATAGCCATTTTA
CAGGTGTGGAACGGGAAGCAGCGCCAGAGCTCCCTTTGTGGAAACAGCCCGAGGTGGTTGAATTGGCTCAACAGATCGGA
AAAACGCTTCTGATTGGTGGCCTGATTCTGTATCTGGTGCTGGGTGTGCTACGTCCCTCGCTCAAGCGCATCAGCCAGCC
CGAACCTCCGTTACTGGCAGAACCGAGGAACGATGACCAGGATGTGCAACCTGTTCCCGCATTGAAACCTGCGGCACAAC
CTTCCGAATACGAGCAAAGACTGACGCTCGCGCGCCAGCTTGCGAAAAAGGAGCCGAAGCTGGTGGCAAACGTTGTCAAG
CAATGGGTGGCTGGTCATGAATGA

Upstream 100 bases:

>100_bases
GTGATAATGGCGACTGTCAAGAGGTTTGCCAAGGTACGCCTATCCTATATAGGACAAAGAAACGCGATTTGATCTGGAGC
ACAGGACTGGGGAGAAACAG

Downstream 100 bases:

>100_bases
GGAGGGTATCTACAAGAGTGCGGTCCTGCTCCTGTCCTTGGGCGAAGACGAGGCGGTGCAGGTACTGAAACATCTCAATC
AGCTGGAGGTTCAAAAACTC

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 567; Mature: 566

Protein sequence:

>567_residues
MTAGANATTMNSAMNTAANNAAKGAVSSIQGGLQSFLTRLSSQQKIGLMLTVSSLVALLAAGWMWTQGPDYKVLFSNVSD
GDGGAIIASLQQMNVPYKFAEGGGAILVPSSQVHDTRLKLASQGLPRGGLVGFELMENQKFGTSQFLEQVNFQRALEGEL
ARSVQALAAVDSARVHLAIPKQSVFMREQQKPSASVLVNLYPGRTLDPQQVNAIVHLVSSSVPNLHVKNVTVVDQQGDLL
TRIDESNPNTGLDPAQLKYLHALEQSYIKRIEAILIPIVGQENVRAQVAAEVDFAHSEQTSETYRPNQDPAGSTIRSQQS
SESTNVGTQNEGGVPGALSNQPPVPATAPVTTPPAPPGATASPSRAPSSTRKDATINYEVDKTIQHVRKPVGGIKRLSVA
VVVNHRRVVDENGVVSAKPLSEVEKSQITDLVKEAVGFSRERGDTLNVVNSHFTGVEREAAPELPLWKQPEVVELAQQIG
KTLLIGGLILYLVLGVLRPSLKRISQPEPPLLAEPRNDDQDVQPVPALKPAAQPSEYEQRLTLARQLAKKEPKLVANVVK
QWVAGHE

Sequences:

>Translated_567_residues
MTAGANATTMNSAMNTAANNAAKGAVSSIQGGLQSFLTRLSSQQKIGLMLTVSSLVALLAAGWMWTQGPDYKVLFSNVSD
GDGGAIIASLQQMNVPYKFAEGGGAILVPSSQVHDTRLKLASQGLPRGGLVGFELMENQKFGTSQFLEQVNFQRALEGEL
ARSVQALAAVDSARVHLAIPKQSVFMREQQKPSASVLVNLYPGRTLDPQQVNAIVHLVSSSVPNLHVKNVTVVDQQGDLL
TRIDESNPNTGLDPAQLKYLHALEQSYIKRIEAILIPIVGQENVRAQVAAEVDFAHSEQTSETYRPNQDPAGSTIRSQQS
SESTNVGTQNEGGVPGALSNQPPVPATAPVTTPPAPPGATASPSRAPSSTRKDATINYEVDKTIQHVRKPVGGIKRLSVA
VVVNHRRVVDENGVVSAKPLSEVEKSQITDLVKEAVGFSRERGDTLNVVNSHFTGVEREAAPELPLWKQPEVVELAQQIG
KTLLIGGLILYLVLGVLRPSLKRISQPEPPLLAEPRNDDQDVQPVPALKPAAQPSEYEQRLTLARQLAKKEPKLVANVVK
QWVAGHE
>Mature_566_residues
TAGANATTMNSAMNTAANNAAKGAVSSIQGGLQSFLTRLSSQQKIGLMLTVSSLVALLAAGWMWTQGPDYKVLFSNVSDG
DGGAIIASLQQMNVPYKFAEGGGAILVPSSQVHDTRLKLASQGLPRGGLVGFELMENQKFGTSQFLEQVNFQRALEGELA
RSVQALAAVDSARVHLAIPKQSVFMREQQKPSASVLVNLYPGRTLDPQQVNAIVHLVSSSVPNLHVKNVTVVDQQGDLLT
RIDESNPNTGLDPAQLKYLHALEQSYIKRIEAILIPIVGQENVRAQVAAEVDFAHSEQTSETYRPNQDPAGSTIRSQQSS
ESTNVGTQNEGGVPGALSNQPPVPATAPVTTPPAPPGATASPSRAPSSTRKDATINYEVDKTIQHVRKPVGGIKRLSVAV
VVNHRRVVDENGVVSAKPLSEVEKSQITDLVKEAVGFSRERGDTLNVVNSHFTGVEREAAPELPLWKQPEVVELAQQIGK
TLLIGGLILYLVLGVLRPSLKRISQPEPPLLAEPRNDDQDVQPVPALKPAAQPSEYEQRLTLARQLAKKEPKLVANVVKQ
WVAGHE

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=542, Percent_Identity=47.4169741697417, Blast_Score=459, Evalue=1e-130,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 60836; Mature: 60705

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAGANATTMNSAMNTAANNAAKGAVSSIQGGLQSFLTRLSSQQKIGLMLTVSSLVALLA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHH
AGWMWTQGPDYKVLFSNVSDGDGGAIIASLQQMNVPYKFAEGGGAILVPSSQVHDTRLKL
CCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEECCCCEEEECCCCHHHHHHHH
ASQGLPRGGLVGFELMENQKFGTSQFLEQVNFQRALEGELARSVQALAAVDSARVHLAIP
HHCCCCCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
KQSVFMREQQKPSASVLVNLYPGRTLDPQQVNAIVHLVSSSVPNLHVKNVTVVDQQGDLL
CHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEECEEEEEECCCCHH
TRIDESNPNTGLDPAQLKYLHALEQSYIKRIEAILIPIVGQENVRAQVAAEVDFAHSEQT
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEHEEHHHHHCCCCC
SETYRPNQDPAGSTIRSQQSSESTNVGTQNEGGVPGALSNQPPVPATAPVTTPPAPPGAT
HHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ASPSRAPSSTRKDATINYEVDKTIQHVRKPVGGIKRLSVAVVVNHRRVVDENGVVSAKPL
CCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHEEEEEEEECCEEECCCCCCCCCCH
SEVEKSQITDLVKEAVGFSRERGDTLNVVNSHFTGVEREAAPELPLWKQPEVVELAQQIG
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHCCCCCCCCCCCHHHHHHHHHH
KTLLIGGLILYLVLGVLRPSLKRISQPEPPLLAEPRNDDQDVQPVPALKPAAQPSEYEQR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
LTLARQLAKKEPKLVANVVKQWVAGHE
HHHHHHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TAGANATTMNSAMNTAANNAAKGAVSSIQGGLQSFLTRLSSQQKIGLMLTVSSLVALLA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHH
AGWMWTQGPDYKVLFSNVSDGDGGAIIASLQQMNVPYKFAEGGGAILVPSSQVHDTRLKL
CCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCCEEEECCCCEEEECCCCHHHHHHHH
ASQGLPRGGLVGFELMENQKFGTSQFLEQVNFQRALEGELARSVQALAAVDSARVHLAIP
HHCCCCCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
KQSVFMREQQKPSASVLVNLYPGRTLDPQQVNAIVHLVSSSVPNLHVKNVTVVDQQGDLL
CHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEECEEEEEECCCCHH
TRIDESNPNTGLDPAQLKYLHALEQSYIKRIEAILIPIVGQENVRAQVAAEVDFAHSEQT
HHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCEEHEEHHHHHCCCCC
SETYRPNQDPAGSTIRSQQSSESTNVGTQNEGGVPGALSNQPPVPATAPVTTPPAPPGAT
HHHCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ASPSRAPSSTRKDATINYEVDKTIQHVRKPVGGIKRLSVAVVVNHRRVVDENGVVSAKPL
CCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHEEEEEEEECCEEECCCCCCCCCCH
SEVEKSQITDLVKEAVGFSRERGDTLNVVNSHFTGVEREAAPELPLWKQPEVVELAQQIG
HHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHCCCCCCCCCCCHHHHHHHHHH
KTLLIGGLILYLVLGVLRPSLKRISQPEPPLLAEPRNDDQDVQPVPALKPAAQPSEYEQR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
LTLARQLAKKEPKLVANVVKQWVAGHE
HHHHHHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1551848; 8224881 [H]