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The map label for this gene is gacS [H]

Identifier: 82702416

GI number: 82702416

Start: 1479126

End: 1480838

Strand: Reverse

Name: gacS [H]

Synonym: Nmul_A1287

Alternate gene names: 82702416

Gene position: 1480838-1479126 (Counterclockwise)

Preceding gene: 82702417

Following gene: 82702415

Centisome position: 46.51

GC content: 53.65

Gene sequence:

>1713_bases
ATGACCAAACCCCTGATTCGCGTTCTACTCGTCGAAGACGATGCCGTGGATCGTATGGCGTGTCGACGTGCTCTTGCCCG
GGACCCCGACTACGAATTCGTGCTTTCCGAAGCCGAATCAGGCCGGGAGGGATTGCAACTTGCCCATGAGCAGAAGCCGG
ACTGCGTCCTGCTTGATTACCATTTGCCGGATGTGAATGGTCTTGAGTTTCTGGCAGCGCTGACCGACGAAAGTGGCGAT
GTTTCCATTCCGGTCATGATGCTGACAGGTACGGACAATGCCGCCATTGCGGTAGAAGCTATGAAGCGCGGGGCGAAGGA
TTACCTGATCAAGGACCTCGAGCACCAATACCTTGAATTATTGCCTGCCGTTATCCAGCGCGTACTGAGCGACCGCCGGA
TTCGGCTGGAAAAAAAGCAGGCGGAAGAGAAACTCGCCCAGGCGGAAGCCAAATATCGCTCTCTGGTCGAAACCATTCCG
GCAATCGTCTACATTGCTGCACTGGATGGGAGCAACCGTTTCCTTTACGTCAGCTCGCGTATCAATATGCTTGGTTTTTC
AGCGGAACAGTGGTTGAACGATCCGACTATCCTGCTTGCACAGATTCATCCGGACGACCGGTCGCACGCACTCGAGGAGC
GGGCAAAAAGCCGCGCAACGTGCGCCCCTCTGCGCTGTGAGTACCGTTTGCTTACTCAGGATGAAAGAGTCTTGTGGTTC
CGCGACGAGGCGAATGTTGTGCGGGATGAATCGGGACGCTCACTGTTTCTGCAGGGTATCCTGGTTGATATCACGGAAAG
CAAACAGGCAGAAGAGGAGTTGAAGCAGCATCGTTTTCGTCTCGAAGAGCTCGTGGCCAAGCGCACCGATGAACTGGCGC
GGGTGAACGAAGAGTTGCGGCGCGATATCACCCACCGCAAGCTGATCGAAGAAGAACTGATCAAAGCCAAGACAGAGGCG
GAAAAGGCCAATCTGGCCAAATCGGATTTTCTTTCCAGCATGAGCCACGAATTACGCTCACCCCTGAATGTCATGCTTGG
TTTCGCCCAATTGATGGAATCGAGTTCTCCGCCGCCAACATTCACGCAAATGACCATGCTCAAGGAGATCAATTCTGCAG
GATGGTATCTGCTTGAATTGATAAATAAAATCCTCGATCTCGCCGCAATCGAGTCCGGCAAACTGATTGTTGCGCAGGAA
CCGATGTGCATCGGAGAGGTGATGGCCGAGTCCCGGACCATGGTCGAACCGCAGGCCCAGGAGCAAAACATACGGCTGAT
CTTTCCGCCATCATCGGACATGAACTTCTTCGTTCGGGCAGACCGAACGCGGGTGAAGCAGGTTCTGATCAATCTTCTTT
CCAACGCAATCAAATACAACCGCGAGGGTGGCATGGTCGAAGTGAGTTGCTCCATGACCAAACCCGGGCGTCTGCGAATA
AGTGTGCGCGATACGGGGACGGGGCTGCCGCCGGAAAAACTGACGCAGCTTTTTCAGCAATTCAACCGGCTTGGACAGGA
AGCAGGGTCTGTGGAAGGCACAGGCATTGGACTGGTAGTGACGAAACAACTGGTTGAACTGATGGGCGGATCAATCGGTG
TGGAAAGCACTGTCGATGTCGGCAGTGTGTTCTGGTTTGAACTCATGGTTGATGAACCTTCCCAGGCTGCTTCCGATATA
GATAAAAACGAGGAGTTCAGTACTGAAGGTTAA

Upstream 100 bases:

>100_bases
CGTCGAAGTCATGCGTTCCATCGATGCTTACTGGACCATCAGTGAGATGCCATGATAAAGGTTTGATCAACTCCCTGATT
TTGCTAAAATTCCTCCACTT

Downstream 100 bases:

>100_bases
GATAACGTCCCTGACTCCCGGCTCCCCATCCCCCGTCCATTCTTCAGCAGATGAAAATGCCTCTACCGGATACTTCACAT
CCTGCTGAACACGCGGGAAA

Product: multi-sensor signal transduction histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 570; Mature: 569

Protein sequence:

>570_residues
MTKPLIRVLLVEDDAVDRMACRRALARDPDYEFVLSEAESGREGLQLAHEQKPDCVLLDYHLPDVNGLEFLAALTDESGD
VSIPVMMLTGTDNAAIAVEAMKRGAKDYLIKDLEHQYLELLPAVIQRVLSDRRIRLEKKQAEEKLAQAEAKYRSLVETIP
AIVYIAALDGSNRFLYVSSRINMLGFSAEQWLNDPTILLAQIHPDDRSHALEERAKSRATCAPLRCEYRLLTQDERVLWF
RDEANVVRDESGRSLFLQGILVDITESKQAEEELKQHRFRLEELVAKRTDELARVNEELRRDITHRKLIEEELIKAKTEA
EKANLAKSDFLSSMSHELRSPLNVMLGFAQLMESSSPPPTFTQMTMLKEINSAGWYLLELINKILDLAAIESGKLIVAQE
PMCIGEVMAESRTMVEPQAQEQNIRLIFPPSSDMNFFVRADRTRVKQVLINLLSNAIKYNREGGMVEVSCSMTKPGRLRI
SVRDTGTGLPPEKLTQLFQQFNRLGQEAGSVEGTGIGLVVTKQLVELMGGSIGVESTVDVGSVFWFELMVDEPSQAASDI
DKNEEFSTEG

Sequences:

>Translated_570_residues
MTKPLIRVLLVEDDAVDRMACRRALARDPDYEFVLSEAESGREGLQLAHEQKPDCVLLDYHLPDVNGLEFLAALTDESGD
VSIPVMMLTGTDNAAIAVEAMKRGAKDYLIKDLEHQYLELLPAVIQRVLSDRRIRLEKKQAEEKLAQAEAKYRSLVETIP
AIVYIAALDGSNRFLYVSSRINMLGFSAEQWLNDPTILLAQIHPDDRSHALEERAKSRATCAPLRCEYRLLTQDERVLWF
RDEANVVRDESGRSLFLQGILVDITESKQAEEELKQHRFRLEELVAKRTDELARVNEELRRDITHRKLIEEELIKAKTEA
EKANLAKSDFLSSMSHELRSPLNVMLGFAQLMESSSPPPTFTQMTMLKEINSAGWYLLELINKILDLAAIESGKLIVAQE
PMCIGEVMAESRTMVEPQAQEQNIRLIFPPSSDMNFFVRADRTRVKQVLINLLSNAIKYNREGGMVEVSCSMTKPGRLRI
SVRDTGTGLPPEKLTQLFQQFNRLGQEAGSVEGTGIGLVVTKQLVELMGGSIGVESTVDVGSVFWFELMVDEPSQAASDI
DKNEEFSTEG
>Mature_569_residues
TKPLIRVLLVEDDAVDRMACRRALARDPDYEFVLSEAESGREGLQLAHEQKPDCVLLDYHLPDVNGLEFLAALTDESGDV
SIPVMMLTGTDNAAIAVEAMKRGAKDYLIKDLEHQYLELLPAVIQRVLSDRRIRLEKKQAEEKLAQAEAKYRSLVETIPA
IVYIAALDGSNRFLYVSSRINMLGFSAEQWLNDPTILLAQIHPDDRSHALEERAKSRATCAPLRCEYRLLTQDERVLWFR
DEANVVRDESGRSLFLQGILVDITESKQAEEELKQHRFRLEELVAKRTDELARVNEELRRDITHRKLIEEELIKAKTEAE
KANLAKSDFLSSMSHELRSPLNVMLGFAQLMESSSPPPTFTQMTMLKEINSAGWYLLELINKILDLAAIESGKLIVAQEP
MCIGEVMAESRTMVEPQAQEQNIRLIFPPSSDMNFFVRADRTRVKQVLINLLSNAIKYNREGGMVEVSCSMTKPGRLRIS
VRDTGTGLPPEKLTQLFQQFNRLGQEAGSVEGTGIGLVVTKQLVELMGGSIGVESTVDVGSVFWFELMVDEPSQAASDID
KNEEFSTEG

Specific function: Forms part of a two-component regulatory system gacA/gacS(lemA). May be involved in lesion formation, swarming and in the production of extracellular protease, syringomycin and N- acyl-L-homoserine lactone (acyl-HSL). Required for pathogenicity on bean [H

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789149, Length=265, Percent_Identity=34.3396226415094, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI87081816, Length=277, Percent_Identity=36.8231046931408, Blast_Score=147, Evalue=3e-36,
Organism=Escherichia coli, GI48994928, Length=258, Percent_Identity=32.5581395348837, Blast_Score=130, Evalue=3e-31,
Organism=Escherichia coli, GI145693157, Length=245, Percent_Identity=33.0612244897959, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1788713, Length=266, Percent_Identity=31.203007518797, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1786912, Length=257, Percent_Identity=29.1828793774319, Blast_Score=104, Evalue=2e-23,
Organism=Escherichia coli, GI1790436, Length=303, Percent_Identity=29.042904290429, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786600, Length=248, Percent_Identity=25.4032258064516, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1788393, Length=213, Percent_Identity=25.8215962441315, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1790861, Length=214, Percent_Identity=26.6355140186916, Blast_Score=73, Evalue=6e-14,
Organism=Escherichia coli, GI1786783, Length=220, Percent_Identity=25.9090909090909, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1788279, Length=205, Percent_Identity=28.2926829268293, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1788549, Length=252, Percent_Identity=22.2222222222222, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI1790437, Length=119, Percent_Identity=36.1344537815126, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011006
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR008207
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF01627 Hpt; PF00072 Response_reg [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 64141; Mature: 64010

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS50112 PAS ; PS50113 PAC ; PS50110 RESPONSE_REGULATORY ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPLIRVLLVEDDAVDRMACRRALARDPDYEFVLSEAESGREGLQLAHEQKPDCVLLDY
CCCCCEEEEEECCCHHHHHHHHHHHHCCCCHHEEECCHHCCHHHHHHHHCCCCCEEEEEE
HLPDVNGLEFLAALTDESGDVSIPVMMLTGTDNAAIAVEAMKRGAKDYLIKDLEHQYLEL
CCCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEHHHHHCCHHHHHHHHHHHHHHHH
LPAVIQRVLSDRRIRLEKKQAEEKLAQAEAKYRSLVETIPAIVYIAALDGSNRFLYVSSR
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEECC
INMLGFSAEQWLNDPTILLAQIHPDDRSHALEERAKSRATCAPLRCEYRLLTQDERVLWF
CCHHCCCHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEE
RDEANVVRDESGRSLFLQGILVDITESKQAEEELKQHRFRLEELVAKRTDELARVNEELR
ECCCCCEECCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDITHRKLIEEELIKAKTEAEKANLAKSDFLSSMSHELRSPLNVMLGFAQLMESSSPPPT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FTQMTMLKEINSAGWYLLELINKILDLAAIESGKLIVAQEPMCIGEVMAESRTMVEPQAQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCCCCCCCC
EQNIRLIFPPSSDMNFFVRADRTRVKQVLINLLSNAIKYNREGGMVEVSCSMTKPGRLRI
CCCEEEEECCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEE
SVRDTGTGLPPEKLTQLFQQFNRLGQEAGSVEGTGIGLVVTKQLVELMGGSIGVESTVDV
EEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCH
GSVFWFELMVDEPSQAASDIDKNEEFSTEG
HHEEEEEEEECCCHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TKPLIRVLLVEDDAVDRMACRRALARDPDYEFVLSEAESGREGLQLAHEQKPDCVLLDY
CCCCEEEEEECCCHHHHHHHHHHHHCCCCHHEEECCHHCCHHHHHHHHCCCCCEEEEEE
HLPDVNGLEFLAALTDESGDVSIPVMMLTGTDNAAIAVEAMKRGAKDYLIKDLEHQYLEL
CCCCCCHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEHHHHHCCHHHHHHHHHHHHHHHH
LPAVIQRVLSDRRIRLEKKQAEEKLAQAEAKYRSLVETIPAIVYIAALDGSNRFLYVSSR
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCEEEEEECC
INMLGFSAEQWLNDPTILLAQIHPDDRSHALEERAKSRATCAPLRCEYRLLTQDERVLWF
CCHHCCCHHHHCCCCEEEEEEECCCCHHHHHHHHHHCCCCCCCCEEEEEEECCCCEEEEE
RDEANVVRDESGRSLFLQGILVDITESKQAEEELKQHRFRLEELVAKRTDELARVNEELR
ECCCCCEECCCCCEEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDITHRKLIEEELIKAKTEAEKANLAKSDFLSSMSHELRSPLNVMLGFAQLMESSSPPPT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
FTQMTMLKEINSAGWYLLELINKILDLAAIESGKLIVAQEPMCIGEVMAESRTMVEPQAQ
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCCCCCCCC
EQNIRLIFPPSSDMNFFVRADRTRVKQVLINLLSNAIKYNREGGMVEVSCSMTKPGRLRI
CCCEEEEECCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEE
SVRDTGTGLPPEKLTQLFQQFNRLGQEAGSVEGTGIGLVVTKQLVELMGGSIGVESTVDV
EEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCH
GSVFWFELMVDEPSQAASDIDKNEEFSTEG
HHEEEEEEEECCCHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1314807 [H]