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The map label for this gene is sbp [H]

Identifier: 82702321

GI number: 82702321

Start: 1380540

End: 1381556

Strand: Direct

Name: sbp [H]

Synonym: Nmul_A1192

Alternate gene names: 82702321

Gene position: 1380540-1381556 (Clockwise)

Preceding gene: 82702318

Following gene: 82702322

Centisome position: 43.36

GC content: 49.75

Gene sequence:

>1017_bases
ATGCGTTCATATCACTCCCGGCTGGTTTTTGCGATTCTGGCAATTCTGCTTAGCTTCAGTGCAGCGGTTGCCGCGCTGCA
TAGCATGTCCGCGAACTACAGTTCCGATGCAAGTCACAAAATGTTCGATGATTTCAACAAGGCGTTTATCAAGCACTGGA
AAACGCGAACGGGTGTGGAGGTGACGATCCGCCATGCGGAAAGCAAGCCAGGCATACCTCTCCGGGCGAATATAGATGGA
CTCGATGTTACGTCAATTGCTCTGTCCTATGATCCGGAGATACTGCAAAAAACCAATAGATTTTCGTTACCTGACTGGCA
AAAACCCCTCCCACGAAAATCACCATATACCTCAACCATTGTCTTTCTGGTGCGCAATGGCAACCCGAAGAAGGTGCGGG
ATTGGAATGATCTCGCGAACCCGGGTATCGAGATTGTAACTTCCAATCCAAAGACTTCGGGGGATGCACGCTGGAGTTAC
CTGGCCGCCTGGGGGTATGCGCTCAAACAGTCCCGAGGAAGTGATGTAACGGCGATCGAATTCATAAAAAAAATATTTGG
CAACGTAAAGACTCTGGAAAACGGAACGCGCAGTTCAGTAATAACTTTCGTCGAACGCGGCAGAGGGGATGTGCTGCTGA
TTTGGGAAAATGAAGCCCACCTGATCGTCAAAGAGCATGGTGGAGACAAATTTGAGATCATTACCCCCTCCTTCTCAATC
CTGGCCGAACCCACTGTGAACATAGTAAGTGATGCCGCCTCACAGAATGGAACGCGTGAAGTGGCCAAGGCTTACGTCGA
TTATCTCTACACACTGAAGGCCCAGGAAATAGCAGCCAGACACTATTATCGCCCGCGTGATGAGAGGATAGCCGCCAGGT
ATTCGAGGCAATTTCCATCTCTCGAATTGTTTACCGTCGATGAAATGTTTGGAGGCTGGAAGGAGGCTCGAAACATCCAT
TTTGCGGACGGCGGCATTTTCGACCGAATTCAAACGCATTCAAGCGCGTTTCACTGA

Upstream 100 bases:

>100_bases
AAGAAAGAACGGTAACCCGCTCTATTTCTTCATTTCCATGCAATGCTGTTGAAATGAACCGGTGTTAGATTTCCTGGTAT
CAATCCACAGGAGGAAAAAG

Downstream 100 bases:

>100_bases
AATTGCAGCCGCCTGTCAGTATGGGAGTATTTCCAACTGACATTCATGCCTACCACAATCTTCTGCAAAAATTCCTCCTA
TCTTTCCGACTGTAATTTCC

Product: thiosulfate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MRSYHSRLVFAILAILLSFSAAVAALHSMSANYSSDASHKMFDDFNKAFIKHWKTRTGVEVTIRHAESKPGIPLRANIDG
LDVTSIALSYDPEILQKTNRFSLPDWQKPLPRKSPYTSTIVFLVRNGNPKKVRDWNDLANPGIEIVTSNPKTSGDARWSY
LAAWGYALKQSRGSDVTAIEFIKKIFGNVKTLENGTRSSVITFVERGRGDVLLIWENEAHLIVKEHGGDKFEIITPSFSI
LAEPTVNIVSDAASQNGTREVAKAYVDYLYTLKAQEIAARHYYRPRDERIAARYSRQFPSLELFTVDEMFGGWKEARNIH
FADGGIFDRIQTHSSAFH

Sequences:

>Translated_338_residues
MRSYHSRLVFAILAILLSFSAAVAALHSMSANYSSDASHKMFDDFNKAFIKHWKTRTGVEVTIRHAESKPGIPLRANIDG
LDVTSIALSYDPEILQKTNRFSLPDWQKPLPRKSPYTSTIVFLVRNGNPKKVRDWNDLANPGIEIVTSNPKTSGDARWSY
LAAWGYALKQSRGSDVTAIEFIKKIFGNVKTLENGTRSSVITFVERGRGDVLLIWENEAHLIVKEHGGDKFEIITPSFSI
LAEPTVNIVSDAASQNGTREVAKAYVDYLYTLKAQEIAARHYYRPRDERIAARYSRQFPSLELFTVDEMFGGWKEARNIH
FADGGIFDRIQTHSSAFH
>Mature_338_residues
MRSYHSRLVFAILAILLSFSAAVAALHSMSANYSSDASHKMFDDFNKAFIKHWKTRTGVEVTIRHAESKPGIPLRANIDG
LDVTSIALSYDPEILQKTNRFSLPDWQKPLPRKSPYTSTIVFLVRNGNPKKVRDWNDLANPGIEIVTSNPKTSGDARWSY
LAAWGYALKQSRGSDVTAIEFIKKIFGNVKTLENGTRSSVITFVERGRGDVLLIWENEAHLIVKEHGGDKFEIITPSFSI
LAEPTVNIVSDAASQNGTREVAKAYVDYLYTLKAQEIAARHYYRPRDERIAARYSRQFPSLELFTVDEMFGGWKEARNIH
FADGGIFDRIQTHSSAFH

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=321, Percent_Identity=50.4672897196262, Blast_Score=330, Evalue=8e-92,
Organism=Escherichia coli, GI1788765, Length=303, Percent_Identity=38.2838283828383, Blast_Score=217, Evalue=1e-57,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 38130; Mature: 38130

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSYHSRLVFAILAILLSFSAAVAALHSMSANYSSDASHKMFDDFNKAFIKHWKTRTGVE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
VTIRHAESKPGIPLRANIDGLDVTSIALSYDPEILQKTNRFSLPDWQKPLPRKSPYTSTI
EEEEECCCCCCCEEEECCCCCEEEEEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCEEE
VFLVRNGNPKKVRDWNDLANPGIEIVTSNPKTSGDARWSYLAAWGYALKQSRGSDVTAIE
EEEEECCCCCCCCCCHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHH
FIKKIFGNVKTLENGTRSSVITFVERGRGDVLLIWENEAHLIVKEHGGDKFEIITPSFSI
HHHHHHCCCCHHCCCCHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCEEEEECCCHHE
LAEPTVNIVSDAASQNGTREVAKAYVDYLYTLKAQEIAARHYYRPRDERIAARYSRQFPS
EECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
LELFTVDEMFGGWKEARNIHFADGGIFDRIQTHSSAFH
EEEEEHHHHHCCHHHHCEEEEECCCHHHHHHHHCCCCC
>Mature Secondary Structure
MRSYHSRLVFAILAILLSFSAAVAALHSMSANYSSDASHKMFDDFNKAFIKHWKTRTGVE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEE
VTIRHAESKPGIPLRANIDGLDVTSIALSYDPEILQKTNRFSLPDWQKPLPRKSPYTSTI
EEEEECCCCCCCEEEECCCCCEEEEEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCEEE
VFLVRNGNPKKVRDWNDLANPGIEIVTSNPKTSGDARWSYLAAWGYALKQSRGSDVTAIE
EEEEECCCCCCCCCCHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHH
FIKKIFGNVKTLENGTRSSVITFVERGRGDVLLIWENEAHLIVKEHGGDKFEIITPSFSI
HHHHHHCCCCHHCCCCHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCEEEEECCCHHE
LAEPTVNIVSDAASQNGTREVAKAYVDYLYTLKAQEIAARHYYRPRDERIAARYSRQFPS
EECCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
LELFTVDEMFGGWKEARNIHFADGGIFDRIQTHSSAFH
EEEEEHHHHHCCHHHHCEEEEECCCHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]