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The map label for this gene is bioB

Identifier: 82702258

GI number: 82702258

Start: 1298315

End: 1299343

Strand: Direct

Name: bioB

Synonym: Nmul_A1129

Alternate gene names: 82702258

Gene position: 1298315-1299343 (Clockwise)

Preceding gene: 82702255

Following gene: 82702259

Centisome position: 40.77

GC content: 56.27

Gene sequence:

>1029_bases
ATGAGCCAATCAGCTTCCGGTATCGTCAACATTGAAACGTCTTCGGGCGGAGCAGTCGACAGTTTCGCTGACAAACTTTC
CGGCATCAGTGCTGATTTTCGGCGGTGGGACGTTCGCCGCGTCGAAGCGTTGCTCGATCTGCCGTTCAGCGATCTGATTC
ATCGGGCGCAACTGGTTCATCGTCAATATCACGATCCCAACGGAGTGCAACTTTCCACACTGATTTCCGTCAAGACCGGG
GGGTGTCCTGAGGATTGCGGATATTGTCCGCAGGCGGCGCGCTATCATACGAATGTGGAAAATCAGGACATGCTCACGGT
TGAAGACGTGATAGCAGCAGCGGCAGCCGCCAAAGCGCAGGGGGCCACGCGGTTTTGCATGGGCGCAGCGTGGCGCGGTC
CCAAGGAGCGGGATATGAAAAGCGTGGTGGAAATGGTGAGGGCTGTAAAAGCGTTGGGGCTTGAAGCGTGCACGACGTTG
GGCATGTTGAAGCCGGGACAGGCGGAACAGCTCCAAGAGGCGGGTCTCGACTATTACAACCATAACCTCGATACGTCACC
CGAATTTTACGGGGAGATCATTACAACCCGCGATTACGAGGATCGGCTCGACACCTTGCAAAAGGTTCGTCATGCGGGTA
TCAATGTGTGCTGTGGAGGCATTGTGGGCATGGGCGAATCGCGTCGCGCCCGGGCTGGACTCATCGCCCAGTTGGCTAAT
CTCGATCCTTATCCCGAGTCCGTTCCCATCAACCATCTGGTGCAGGTCGAAGGCACGCCGCTTCACGGGACAGAGGCGCT
GGATCCTCTGGAATTCGTGCGCACGATTGCGGCTGCGCGCATTACCATGCCGAAGGCAATGGTAAGGTTATCAGCCGGCA
GGAGAGAAATGCCGGATGCAGTGCAGGCACTGTGTTTTCTCGCTGGCGCCAATTCCATTTTTTACGGCGATAAGCTGCTT
ACTACCGGCAATCCTGAAGCCGAACGGGATAAAGCGTTATTTGACAGGCTGGGGCTGCATTCCATATAG

Upstream 100 bases:

>100_bases
ACTAAAGACGAGGCGGGAAAATGAGAGCCATTTGGCTTTCATTTCGTTTATCGTGAACAGGGTTCAGACAAATCGATTGT
CAAACATTGAAGGGTAGGAT

Downstream 100 bases:

>100_bases
CAGCTTTTCACCTCGATCTTCTTTATTTTCCACTACCCTGCGGCAAATGATCTTATCGGGCAACCTCTGCTCGTATGCTC
AACGACTTGGATTCAATTCA

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MSQSASGIVNIETSSGGAVDSFADKLSGISADFRRWDVRRVEALLDLPFSDLIHRAQLVHRQYHDPNGVQLSTLISVKTG
GCPEDCGYCPQAARYHTNVENQDMLTVEDVIAAAAAAKAQGATRFCMGAAWRGPKERDMKSVVEMVRAVKALGLEACTTL
GMLKPGQAEQLQEAGLDYYNHNLDTSPEFYGEIITTRDYEDRLDTLQKVRHAGINVCCGGIVGMGESRRARAGLIAQLAN
LDPYPESVPINHLVQVEGTPLHGTEALDPLEFVRTIAAARITMPKAMVRLSAGRREMPDAVQALCFLAGANSIFYGDKLL
TTGNPEAERDKALFDRLGLHSI

Sequences:

>Translated_342_residues
MSQSASGIVNIETSSGGAVDSFADKLSGISADFRRWDVRRVEALLDLPFSDLIHRAQLVHRQYHDPNGVQLSTLISVKTG
GCPEDCGYCPQAARYHTNVENQDMLTVEDVIAAAAAAKAQGATRFCMGAAWRGPKERDMKSVVEMVRAVKALGLEACTTL
GMLKPGQAEQLQEAGLDYYNHNLDTSPEFYGEIITTRDYEDRLDTLQKVRHAGINVCCGGIVGMGESRRARAGLIAQLAN
LDPYPESVPINHLVQVEGTPLHGTEALDPLEFVRTIAAARITMPKAMVRLSAGRREMPDAVQALCFLAGANSIFYGDKLL
TTGNPEAERDKALFDRLGLHSI
>Mature_341_residues
SQSASGIVNIETSSGGAVDSFADKLSGISADFRRWDVRRVEALLDLPFSDLIHRAQLVHRQYHDPNGVQLSTLISVKTGG
CPEDCGYCPQAARYHTNVENQDMLTVEDVIAAAAAAKAQGATRFCMGAAWRGPKERDMKSVVEMVRAVKALGLEACTTLG
MLKPGQAEQLQEAGLDYYNHNLDTSPEFYGEIITTRDYEDRLDTLQKVRHAGINVCCGGIVGMGESRRARAGLIAQLANL
DPYPESVPINHLVQVEGTPLHGTEALDPLEFVRTIAAARITMPKAMVRLSAGRREMPDAVQALCFLAGANSIFYGDKLLT
TGNPEAERDKALFDRLGLHSI

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=306, Percent_Identity=61.7647058823529, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6321725, Length=314, Percent_Identity=49.0445859872611, Blast_Score=320, Evalue=2e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_NITMU (Q2Y9Y9)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411824.1
- ProteinModelPortal:   Q2Y9Y9
- SMR:   Q2Y9Y9
- STRING:   Q2Y9Y9
- GeneID:   3784242
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A1129
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- PhylomeDB:   Q2Y9Y9
- ProtClustDB:   CLSK2298556
- BioCyc:   NMUL323848:NMUL_A1129-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 37173; Mature: 37041

Theoretical pI: Translated: 5.84; Mature: 5.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSASGIVNIETSSGGAVDSFADKLSGISADFRRWDVRRVEALLDLPFSDLIHRAQLVH
CCCCCCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RQYHDPNGVQLSTLISVKTGGCPEDCGYCPQAARYHTNVENQDMLTVEDVIAAAAAAKAQ
HHCCCCCCCEEEEEEEECCCCCCHHCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHC
GATRFCMGAAWRGPKERDMKSVVEMVRAVKALGLEACTTLGMLKPGQAEQLQEAGLDYYN
CCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHC
HNLDTSPEFYGEIITTRDYEDRLDTLQKVRHAGINVCCGGIVGMGESRRARAGLIAQLAN
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEECCHHCCCCCCHHHHHHHHHHHC
LDPYPESVPINHLVQVEGTPLHGTEALDPLEFVRTIAAARITMPKAMVRLSAGRREMPDA
CCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VQALCFLAGANSIFYGDKLLTTGNPEAERDKALFDRLGLHSI
HHHHHHHHCCCCEEECCEEEECCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SQSASGIVNIETSSGGAVDSFADKLSGISADFRRWDVRRVEALLDLPFSDLIHRAQLVH
CCCCCCEEEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RQYHDPNGVQLSTLISVKTGGCPEDCGYCPQAARYHTNVENQDMLTVEDVIAAAAAAKAQ
HHCCCCCCCEEEEEEEECCCCCCHHCCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHC
GATRFCMGAAWRGPKERDMKSVVEMVRAVKALGLEACTTLGMLKPGQAEQLQEAGLDYYN
CCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHC
HNLDTSPEFYGEIITTRDYEDRLDTLQKVRHAGINVCCGGIVGMGESRRARAGLIAQLAN
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCEEECCHHCCCCCCHHHHHHHHHHHC
LDPYPESVPINHLVQVEGTPLHGTEALDPLEFVRTIAAARITMPKAMVRLSAGRREMPDA
CCCCCCCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
VQALCFLAGANSIFYGDKLLTTGNPEAERDKALFDRLGLHSI
HHHHHHHHCCCCEEECCEEEECCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA