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The map label for this gene is sspA [H]

Identifier: 82702131

GI number: 82702131

Start: 1163252

End: 1163851

Strand: Reverse

Name: sspA [H]

Synonym: Nmul_A1002

Alternate gene names: 82702131

Gene position: 1163851-1163252 (Counterclockwise)

Preceding gene: 82702132

Following gene: 82702130

Centisome position: 36.55

GC content: 55.67

Gene sequence:

>600_bases
ATGATGACTTTGTACTCAGCCACAACCTGCCCGTACAGCCATCGTTGCCGGATCGTGCTGTACGAAAAGGGTATGGATTT
TCAGATCATTGATGTGGATCTGCATAACAGGCCTGAAGATCTGGCAGTGATGAGCCCGTATGGTCGCGTCCCCGTCCTGG
TGGAGCGCGACCTCGTCCTGTACGAATCCAATATCATCAACGAGTATATTGACGACCGCTTCCCTCACCCGCAACTGATG
CCCGCGGACCCCGTGATGCGGGCGCGGGCCCGTCTGTTTCTGCACCGCTTTGAACAGGAACTTTTCTGTCATCTCGAAGC
AGTGGAGCATGGCAACCAGAAAGCGGCGGAAAAAGCGCGCGCAACGATTCGCGATAACCTGACCCTCATCGCCCCTGTCT
TTGCCAAACAGAAACATATGCTGGGGGATGAATTTTCCATGCTCGATGTCGCCATAGCACCCCTGCTGTGGCGGCTCGAT
CATTACGGCATCCAGCTACCCAAGCAAGCCGCGCCCCTGCTGAAATTTGCCGAGCGCCTGTTCAGCAGACCGGCATTCAT
CGATGCGCTCACAGCCTCTGAAAAGGCCATGCGCAAATGA

Upstream 100 bases:

>100_bases
CTGACCTATGCCCTCAAGAAGGAATACTGGAAGGATGTTCATTGAGATTGATCATTATCCAGAAAGCAGTCGACTTGATA
TTGCTGGGAATTAACCGGTC

Downstream 100 bases:

>100_bases
GAGAGCTTTCCACCAAGCCTTATCTGATTCGCGCCATTTACGAATGGTGCTCGGATTGCGGTTATACGCCTTACATCTCG
GTTAAAGTGGATGACAACAC

Product: glutathione S-transferase-like protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MMTLYSATTCPYSHRCRIVLYEKGMDFQIIDVDLHNRPEDLAVMSPYGRVPVLVERDLVLYESNIINEYIDDRFPHPQLM
PADPVMRARARLFLHRFEQELFCHLEAVEHGNQKAAEKARATIRDNLTLIAPVFAKQKHMLGDEFSMLDVAIAPLLWRLD
HYGIQLPKQAAPLLKFAERLFSRPAFIDALTASEKAMRK

Sequences:

>Translated_199_residues
MMTLYSATTCPYSHRCRIVLYEKGMDFQIIDVDLHNRPEDLAVMSPYGRVPVLVERDLVLYESNIINEYIDDRFPHPQLM
PADPVMRARARLFLHRFEQELFCHLEAVEHGNQKAAEKARATIRDNLTLIAPVFAKQKHMLGDEFSMLDVAIAPLLWRLD
HYGIQLPKQAAPLLKFAERLFSRPAFIDALTASEKAMRK
>Mature_199_residues
MMTLYSATTCPYSHRCRIVLYEKGMDFQIIDVDLHNRPEDLAVMSPYGRVPVLVERDLVLYESNIINEYIDDRFPHPQLM
PADPVMRARARLFLHRFEQELFCHLEAVEHGNQKAAEKARATIRDNLTLIAPVFAKQKHMLGDEFSMLDVAIAPLLWRLD
HYGIQLPKQAAPLLKFAERLFSRPAFIDALTASEKAMRK

Specific function: Forms an equimolar complex with the RNA polymerase holoenzyme (RNAP) but not with the core enzyme [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI38016131, Length=175, Percent_Identity=33.7142857142857, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI300360569, Length=166, Percent_Identity=34.3373493975904, Blast_Score=93, Evalue=2e-19,
Organism=Homo sapiens, GI4758484, Length=195, Percent_Identity=29.2307692307692, Blast_Score=92, Evalue=4e-19,
Organism=Homo sapiens, GI300360534, Length=189, Percent_Identity=29.1005291005291, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI300360567, Length=165, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=3e-18,
Organism=Homo sapiens, GI300360571, Length=156, Percent_Identity=33.974358974359, Blast_Score=85, Evalue=4e-17,
Organism=Homo sapiens, GI300360532, Length=193, Percent_Identity=27.979274611399, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1789624, Length=199, Percent_Identity=45.7286432160804, Blast_Score=186, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI25144449, Length=162, Percent_Identity=27.7777777777778, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17551302, Length=199, Percent_Identity=30.1507537688442, Blast_Score=67, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24660979, Length=162, Percent_Identity=30.2469135802469, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21355779, Length=101, Percent_Identity=37.6237623762376, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21355775, Length=169, Percent_Identity=28.9940828402367, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI21355765, Length=166, Percent_Identity=28.3132530120482, Blast_Score=67, Evalue=5e-12,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: NA

Molecular weight: Translated: 23008; Mature: 23008

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTLYSATTCPYSHRCRIVLYEKGMDFQIIDVDLHNRPEDLAVMSPYGRVPVLVERDLVL
CCEEECCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCEEEECCCCCCCEEEECCEEE
YESNIINEYIDDRFPHPQLMPADPVMRARARLFLHRFEQELFCHLEAVEHGNQKAAEKAR
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ATIRDNLTLIAPVFAKQKHMLGDEFSMLDVAIAPLLWRLDHYGIQLPKQAAPLLKFAERL
HHHHCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
FSRPAFIDALTASEKAMRK
HCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMTLYSATTCPYSHRCRIVLYEKGMDFQIIDVDLHNRPEDLAVMSPYGRVPVLVERDLVL
CCEEECCCCCCCCCCEEEEEEECCCCEEEEEEECCCCCCCEEEECCCCCCCEEEECCEEE
YESNIINEYIDDRFPHPQLMPADPVMRARARLFLHRFEQELFCHLEAVEHGNQKAAEKAR
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ATIRDNLTLIAPVFAKQKHMLGDEFSMLDVAIAPLLWRLDHYGIQLPKQAAPLLKFAERL
HHHHCCCEEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
FSRPAFIDALTASEKAMRK
HCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]