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The map label for this gene is ppsA [H]

Identifier: 82702127

GI number: 82702127

Start: 1157814

End: 1160201

Strand: Direct

Name: ppsA [H]

Synonym: Nmul_A0998

Alternate gene names: 82702127

Gene position: 1157814-1160201 (Clockwise)

Preceding gene: 82702123

Following gene: 82702128

Centisome position: 36.36

GC content: 55.78

Gene sequence:

>2388_bases
ATGGACGAGTCGCCCTATATAGCCTGGTTTGACACTTTGCGCATGACGGATGTGAGTCGCGTCGGCGGCAAAAACGCCTC
CCTTGGCGAGATGATCACCCAGCTGACCGGGATGGGAATTCGGGTTCCCGGCGGTTTTGCAACCACTGCCGATGCCTATC
GCGATTTCCTGGCTCAGGATGGGCTCGCACAGCGTATCGAGCAAACCCTCGCTGAACTTGATATAGAAGATGTTCAGGCA
CTGGCTGCGGCCGGCACACAAATTCGCTCCTGGATAGTGCAAGCATCGTTTCCACCTCGTCTGGAAAATGAAATTTCTGC
TGCTTACGAGAAAATGCTGGGCGAGGCGGAAGGTGAAATTTCGGTAGCGGTGCGTTCTTCGGCCACTGCCGAAGATCTGG
TGGACGCGTCGTTTGCCGGGCAGCAGGAGACTTATCTCAATGTCCAGGGATTGGATAGCCTTCTGAAAGCGGTCAAGGAT
GTCTTTGCTTCCCTCTACAACGACCGGGCCATCTCTTATCGTGTGCATAAAGGGTTCATTCATTCCGAAGTCGCGCTTTC
GGCCGGAATACAGCGGATGGTGCGCAGCGACATCAGTGCCGCGGGAGTGATTTTTACACTGGATACCGAATCCGGTTTTG
ACCAAGTGGTTTTCATCACCGCCTCCTATGGGCTGGGTGAAACCGTTGTCCAGGGCGCGGTCAATCCCGACGAGTTCTAC
GTCTACAAACCGGCATTGACACAGGGAAGGCCGGCTATCCTGCGGCGTAACCTGGGCTCGAAACTCATCAAGATGCGGTT
TGCTGAAGGCCGCGGCGCCGCCCGAGCGGTTGAGACAGTGGATGTGGAAGAGGGGGATCGTAAACGATTCTCCATTAACG
ATGCAGATGTGGAAGAACTGGCCCGTCATGCTCTCGTGATCGAGCGGCATTATCAAAGACCGATGGATATCGAGTGGGGC
AAGGATGGTGCGGACGGCAAGCTCTATATTCTGCAGGCCCGGCCGGAGACAGTGCAGGCACACGCCGCGATGGATACATT
GCGGCGCTATCGCCTGAAGAGCAAATCCGCGATACTCGTGTCAGGGCGCGCCATAGGCCAGAAAATAGGGAGCGGCCCTG
TTCGCTTGGTTCCCGACGCGCAGGAAATGTCGCGGGTACGCGAGGGTGACGTGCTGGTAACCGATATGACCGATCCTGAT
TGGGAGCCGGTAATGAAACGGGCTGCCGCCATCGTTACCAATCGTGGAGGACGAACCTGCCATGCGGCAATCGTTGCGCG
TGAGCTGGGAATTCCTGCGGTGGTCGGCTGCGGCAATGCCACCGGCTTGTTGACGGAGAATGAGATTGTCACCGTATCCT
GCACCGAAGGGGATACCGGCAATATTTATCAGGGGAAGCTGGAAGTCGAGGTGACCGACATCGCGCTCGAAAGCATGCCG
AAGGCTCCGGTCAAGATCAGTATGAATGTCGGCAATCCCGAGCTTGCCTTCGGGTTTCAACGCATTCCCAACCATGGAGT
GGGGCTGGCGCGGCTTGAGTTCATCATTGCGCGCATGATCGGCCTGCATCCAAAAGCAGTGCTGCAATTTGCCAGGCTTC
CGCAAGACGTGAAGCAGCAGATCGAGGCCCAAACCAGCGGTTACGCCGATCCAGTCAATTTTTACGTGGAAAAGCTTACC
GAGGGAATCGCCACCCTGGGCGCAGCCTTTTTTCCAAAACCTGTAATCGTGCGGATGTCCGATTTCAAATCAAATGAATA
CTCCAGCCTCATCGGCGGCCATGATTATGAACCGAGGGAGGAAAACCCGATGCTTGGTTTCCGCGGTGCTTCACGCTACG
TAGCGGACGAATTCCGGGATTGTTTCGAGCTGGAATGCCGCGCGCTGAAGAAAGCGCGCAACGAAATGGGGCTGACCAAC
CTGGAAATCATGATCCCCTTTGTGCGGACACTGTCCGAGGCCCGACGGGTGGTGGAGCTGCTGGCGGAAAACGGTTTGAA
GCGGGGCGAGAATGGACTGCGTTTGATCATGATGTGCGAGATTCCGTCCAACGCATTGTTGGCGGAACAGTTTCTGGAAT
ATTTTGATGGTTTCTCGATCGGGTCCAACGATCTTACTCAACTTACTCTCGGGCTGGACCGTGACTCCAGCCTCGTTGCG
GAAGCCTTCGACGAACGCGATCCGGCGGTAAGAGCGCTTCTGAGGATGGCTATCCAGGCATGCCGTAAAACCGGCAAATA
TGTGGGAATCTGTGGACAGGGGCCTTCCGACCACCCTGACCTCGCACGATGGCTGATGGAAGAGGGCATTGAAACCCTGT
CGCTTAATCCCGATACCATCATCGATACCTGGTTATATCTGGTAGAGGAGGCAAAAAAACAGCTATAG

Upstream 100 bases:

>100_bases
CATGATTATGAGAAACTATCGATGATTTCCGGGTGGAAATTCCGCTTCAAAATGTAGCACAATTCTTCTTTGGCCAGTAT
TTGCACAATCAGGAGTCAAA

Downstream 100 bases:

>100_bases
TCAAAGAATTCGCCGAGCTCGGCAAGTTTGTCGATCATTTGCCGGACAGCAACTTTCACTTTCATTGTCATAGATTCGAC
ACATCGTTATGAGACCATTG

Product: phosphoenolpyruvate synthase

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 795; Mature: 795

Protein sequence:

>795_residues
MDESPYIAWFDTLRMTDVSRVGGKNASLGEMITQLTGMGIRVPGGFATTADAYRDFLAQDGLAQRIEQTLAELDIEDVQA
LAAAGTQIRSWIVQASFPPRLENEISAAYEKMLGEAEGEISVAVRSSATAEDLVDASFAGQQETYLNVQGLDSLLKAVKD
VFASLYNDRAISYRVHKGFIHSEVALSAGIQRMVRSDISAAGVIFTLDTESGFDQVVFITASYGLGETVVQGAVNPDEFY
VYKPALTQGRPAILRRNLGSKLIKMRFAEGRGAARAVETVDVEEGDRKRFSINDADVEELARHALVIERHYQRPMDIEWG
KDGADGKLYILQARPETVQAHAAMDTLRRYRLKSKSAILVSGRAIGQKIGSGPVRLVPDAQEMSRVREGDVLVTDMTDPD
WEPVMKRAAAIVTNRGGRTCHAAIVARELGIPAVVGCGNATGLLTENEIVTVSCTEGDTGNIYQGKLEVEVTDIALESMP
KAPVKISMNVGNPELAFGFQRIPNHGVGLARLEFIIARMIGLHPKAVLQFARLPQDVKQQIEAQTSGYADPVNFYVEKLT
EGIATLGAAFFPKPVIVRMSDFKSNEYSSLIGGHDYEPREENPMLGFRGASRYVADEFRDCFELECRALKKARNEMGLTN
LEIMIPFVRTLSEARRVVELLAENGLKRGENGLRLIMMCEIPSNALLAEQFLEYFDGFSIGSNDLTQLTLGLDRDSSLVA
EAFDERDPAVRALLRMAIQACRKTGKYVGICGQGPSDHPDLARWLMEEGIETLSLNPDTIIDTWLYLVEEAKKQL

Sequences:

>Translated_795_residues
MDESPYIAWFDTLRMTDVSRVGGKNASLGEMITQLTGMGIRVPGGFATTADAYRDFLAQDGLAQRIEQTLAELDIEDVQA
LAAAGTQIRSWIVQASFPPRLENEISAAYEKMLGEAEGEISVAVRSSATAEDLVDASFAGQQETYLNVQGLDSLLKAVKD
VFASLYNDRAISYRVHKGFIHSEVALSAGIQRMVRSDISAAGVIFTLDTESGFDQVVFITASYGLGETVVQGAVNPDEFY
VYKPALTQGRPAILRRNLGSKLIKMRFAEGRGAARAVETVDVEEGDRKRFSINDADVEELARHALVIERHYQRPMDIEWG
KDGADGKLYILQARPETVQAHAAMDTLRRYRLKSKSAILVSGRAIGQKIGSGPVRLVPDAQEMSRVREGDVLVTDMTDPD
WEPVMKRAAAIVTNRGGRTCHAAIVARELGIPAVVGCGNATGLLTENEIVTVSCTEGDTGNIYQGKLEVEVTDIALESMP
KAPVKISMNVGNPELAFGFQRIPNHGVGLARLEFIIARMIGLHPKAVLQFARLPQDVKQQIEAQTSGYADPVNFYVEKLT
EGIATLGAAFFPKPVIVRMSDFKSNEYSSLIGGHDYEPREENPMLGFRGASRYVADEFRDCFELECRALKKARNEMGLTN
LEIMIPFVRTLSEARRVVELLAENGLKRGENGLRLIMMCEIPSNALLAEQFLEYFDGFSIGSNDLTQLTLGLDRDSSLVA
EAFDERDPAVRALLRMAIQACRKTGKYVGICGQGPSDHPDLARWLMEEGIETLSLNPDTIIDTWLYLVEEAKKQL
>Mature_795_residues
MDESPYIAWFDTLRMTDVSRVGGKNASLGEMITQLTGMGIRVPGGFATTADAYRDFLAQDGLAQRIEQTLAELDIEDVQA
LAAAGTQIRSWIVQASFPPRLENEISAAYEKMLGEAEGEISVAVRSSATAEDLVDASFAGQQETYLNVQGLDSLLKAVKD
VFASLYNDRAISYRVHKGFIHSEVALSAGIQRMVRSDISAAGVIFTLDTESGFDQVVFITASYGLGETVVQGAVNPDEFY
VYKPALTQGRPAILRRNLGSKLIKMRFAEGRGAARAVETVDVEEGDRKRFSINDADVEELARHALVIERHYQRPMDIEWG
KDGADGKLYILQARPETVQAHAAMDTLRRYRLKSKSAILVSGRAIGQKIGSGPVRLVPDAQEMSRVREGDVLVTDMTDPD
WEPVMKRAAAIVTNRGGRTCHAAIVARELGIPAVVGCGNATGLLTENEIVTVSCTEGDTGNIYQGKLEVEVTDIALESMP
KAPVKISMNVGNPELAFGFQRIPNHGVGLARLEFIIARMIGLHPKAVLQFARLPQDVKQQIEAQTSGYADPVNFYVEKLT
EGIATLGAAFFPKPVIVRMSDFKSNEYSSLIGGHDYEPREENPMLGFRGASRYVADEFRDCFELECRALKKARNEMGLTN
LEIMIPFVRTLSEARRVVELLAENGLKRGENGLRLIMMCEIPSNALLAEQFLEYFDGFSIGSNDLTQLTLGLDRDSSLVA
EAFDERDPAVRALLRMAIQACRKTGKYVGICGQGPSDHPDLARWLMEEGIETLSLNPDTIIDTWLYLVEEAKKQL

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=789, Percent_Identity=65.0190114068441, Blast_Score=1078, Evalue=0.0,
Organism=Escherichia coli, GI1788756, Length=408, Percent_Identity=25.9803921568627, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI48994992, Length=375, Percent_Identity=26.9333333333333, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1788726, Length=191, Percent_Identity=32.4607329842932, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1789193, Length=391, Percent_Identity=25.8312020460358, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17564524, Length=233, Percent_Identity=28.755364806867, Blast_Score=71, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 87422; Mature: 87422

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDESPYIAWFDTLRMTDVSRVGGKNASLGEMITQLTGMGIRVPGGFATTADAYRDFLAQD
CCCCCEEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHCC
GLAQRIEQTLAELDIEDVQALAAAGTQIRSWIVQASFPPRLENEISAAYEKMLGEAEGEI
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCE
SVAVRSSATAEDLVDASFAGQQETYLNVQGLDSLLKAVKDVFASLYNDRAISYRVHKGFI
EEEEECCCCHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHCHH
HSEVALSAGIQRMVRSDISAAGVIFTLDTESGFDQVVFITASYGLGETVVQGAVNPDEFY
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEECCCCCHHHHHCCCCCCCEE
VYKPALTQGRPAILRRNLGSKLIKMRFAEGRGAARAVETVDVEEGDRKRFSINDADVEEL
EECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEECCCCCCCEEECCCCCHHHH
ARHALVIERHYQRPMDIEWGKDGADGKLYILQARPETVQAHAAMDTLRRYRLKSKSAILV
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEE
SGRAIGQKIGSGPVRLVPDAQEMSRVREGDVLVTDMTDPDWEPVMKRAAAIVTNRGGRTC
ECHHHHHHCCCCCEEECCCHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCH
HAAIVARELGIPAVVGCGNATGLLTENEIVTVSCTEGDTGNIYQGKLEVEVTDIALESMP
HHHHHHHHCCCCEEEECCCCCCEEECCCEEEEEECCCCCCCEEEEEEEEEEEEEHHHCCC
KAPVKISMNVGNPELAFGFQRIPNHGVGLARLEFIIARMIGLHPKAVLQFARLPQDVKQQ
CCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHH
IEAQTSGYADPVNFYVEKLTEGIATLGAAFFPKPVIVRMSDFKSNEYSSLIGGHDYEPRE
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCCCCCCC
ENPMLGFRGASRYVADEFRDCFELECRALKKARNEMGLTNLEIMIPFVRTLSEARRVVEL
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LAENGLKRGENGLRLIMMCEIPSNALLAEQFLEYFDGFSIGSNDLTQLTLGLDRDSSLVA
HHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHH
EAFDERDPAVRALLRMAIQACRKTGKYVGICGQGPSDHPDLARWLMEEGIETLSLNPDTI
HHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCEECCCCHHH
IDTWLYLVEEAKKQL
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MDESPYIAWFDTLRMTDVSRVGGKNASLGEMITQLTGMGIRVPGGFATTADAYRDFLAQD
CCCCCEEEEEHHHHHHHHHHCCCCCCHHHHHHHHHHCCCEECCCCCCHHHHHHHHHHHCC
GLAQRIEQTLAELDIEDVQALAAAGTQIRSWIVQASFPPRLENEISAAYEKMLGEAEGEI
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCE
SVAVRSSATAEDLVDASFAGQQETYLNVQGLDSLLKAVKDVFASLYNDRAISYRVHKGFI
EEEEECCCCHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHCHH
HSEVALSAGIQRMVRSDISAAGVIFTLDTESGFDQVVFITASYGLGETVVQGAVNPDEFY
HHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCEEEEEEECCCCCHHHHHCCCCCCCEE
VYKPALTQGRPAILRRNLGSKLIKMRFAEGRGAARAVETVDVEEGDRKRFSINDADVEEL
EECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHEEEEECCCCCCCEEECCCCCHHHH
ARHALVIERHYQRPMDIEWGKDGADGKLYILQARPETVQAHAAMDTLRRYRLKSKSAILV
HHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEE
SGRAIGQKIGSGPVRLVPDAQEMSRVREGDVLVTDMTDPDWEPVMKRAAAIVTNRGGRTC
ECHHHHHHCCCCCEEECCCHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHCCCCCCCH
HAAIVARELGIPAVVGCGNATGLLTENEIVTVSCTEGDTGNIYQGKLEVEVTDIALESMP
HHHHHHHHCCCCEEEECCCCCCEEECCCEEEEEECCCCCCCEEEEEEEEEEEEEHHHCCC
KAPVKISMNVGNPELAFGFQRIPNHGVGLARLEFIIARMIGLHPKAVLQFARLPQDVKQQ
CCCEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHH
IEAQTSGYADPVNFYVEKLTEGIATLGAAFFPKPVIVRMSDFKSNEYSSLIGGHDYEPRE
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHCCCCCCCCC
ENPMLGFRGASRYVADEFRDCFELECRALKKARNEMGLTNLEIMIPFVRTLSEARRVVEL
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
LAENGLKRGENGLRLIMMCEIPSNALLAEQFLEYFDGFSIGSNDLTQLTLGLDRDSSLVA
HHHCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHH
EAFDERDPAVRALLRMAIQACRKTGKYVGICGQGPSDHPDLARWLMEEGIETLSLNPDTI
HHHHCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCCCEECCCCHHH
IDTWLYLVEEAKKQL
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10710307 [H]