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The map label for this gene is fadK [H]

Identifier: 82702097

GI number: 82702097

Start: 1124942

End: 1126603

Strand: Reverse

Name: fadK [H]

Synonym: Nmul_A0968

Alternate gene names: 82702097

Gene position: 1126603-1124942 (Counterclockwise)

Preceding gene: 82702098

Following gene: 82702096

Centisome position: 35.38

GC content: 57.1

Gene sequence:

>1662_bases
ATGAACCATTCCGGCATCCTGGATATGGTCCCCGCCGAGGTCCGCGCGCAATGGGCCCGGCAGGGAATCTATCCGAATAA
ATCCCTGTACGAGTTGTTTTGCGAGCGAGTGGAGCAACAGCCGGATAATCCGGCAGTAATATCGCTCGACCATACCACCA
GTTACGCAGCGTTGCTGGACAAGGTCCATCGCCTGGCAACCAGTTTCCAGGAATTGGGCATTGTTGCGGGCGATGTAATC
TCATACCAGCTTCATAACGACTGGCGGAGCTGCGCGATCGACCTGGCGGCGGCCGCGCTTGGAGCCATCGTGGCACCTTT
TCCGCCAGGCCGCGGCCGCCTCGATATCCAGTCCCTGCTCAGACGCTGTGACGCCCGTGCAATTATTGTCGAGCGCGAAT
ATGGAAAAACCGACCTTTGCGAATTGATCGAATCCATACGCCCCACTTTGCTTTCACTGCGCATCCTCGTGGTCGATGGC
GCAGCCGGAGACGGTTGGCACGCACTGGATGAATTGTTCCGGCCCGCCTCCATTGAACCGGACCTGCCGACAGTCTGCCC
CGATTCACCTGCCCGTTTCCTGATCTCATCCGGCACGGAATCCGAGCCCAAATGGGTGGCTTATTCTCACAACGCGCTGG
CGGGCGGACGAGGGCGATTTCTGCAACGAATTCATCCCGAAGGAAAGACTTTCAGGGGTCTTTACCTGATGCCGCTCGGC
ACCGCATTCGGCTCCACGGCGACATTCGGGGTTTTATCCTGGCTGGGCGGCTCCCTCATTGTCCTGCGGCAATTTGACGT
TGCAGCTGCTATTCAAGCCCTCGCGGAACTGAAGCCCACGCATATATTGGGCGTTCCCACGATGTTTCAACGCATTGCCG
CAGACCCTGCGTTGACGCAGGCGGATACGTCCAGCCTGGTTGCCATCATCAGCGGCGGCGCGAAAATCGATGAAACCTCC
ATTCGCCGATGTACGAAGGCGTTCCGATGTGGATTCATCAGCTTGTATGGTTCTGCCGATGGCGTCAATTGTCATACGAC
CCTGGATGATGACCTGGAAACCATTATCAGGACGGCGGGAAGGCCCAATCCGGAAATCTGTTCCATTCGTATAATCGATG
ACCAGAAGCAGGAAGTACCGCAAGGTTGCATAGGAGAAATAGCGGCCAGGGGTCCGATAAGTCCCATGCAGTACGTCAAT
GACCCGGATCTCGACGCCCTGTACCGTGACCAGGAGGGATGGGTGTATACCGGTGACCTCGGCCTTATTGATGAAGAGGG
CCATCTGGTGCTATCCGGCCGCAAGAAAGACATCATCATTCGGGGCGGCGTCAATATCAGTCCCGCTCAAATTGAAAACA
TTGCTGTTTCCCATCCGGCGGTTGTCAGCGCAGCCTGTGTTCCGGTGCCCGACGCGGACCTGGGACACAGGGTTTGCCTC
TGTCTCGTCACGAGAGAGGGAGCGGAACGTCCGTCACTTTCCCAGTTCACCCGTTTTCTCCATGAAAAGGGCCTGGAGAC
AAGCAAGCTTCCCGAATACCTGCGCTATTACCGCCAGCTGCCCCTCAGCCCTGCGGGAAAAATCGATAAGAAGCGGCTGA
CTACCGAAATCGAATTCACGGAACATCCCGCCCACCGGAGTCATCCCGAATGGGCACATTGA

Upstream 100 bases:

>100_bases
TCGTCATCGAAGATCTTGTCGACCTTCAAACCCTGGCGCATCTGCAACCAGGGCTGACCTCCGGTTCTTATACCCAAGTC
AATTCTCCCTGGAGATTTAA

Downstream 100 bases:

>100_bases
TGCTGCAAGCATATCAGGAGCAAGCATCCTCCCATACCGAAGCAGCCCGATCTCTTTCGTGCGAGGTAAGAAAGTTCGTC
GACAAGGTAATCATCCCTTG

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Acyl-CoA synthetase [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MNHSGILDMVPAEVRAQWARQGIYPNKSLYELFCERVEQQPDNPAVISLDHTTSYAALLDKVHRLATSFQELGIVAGDVI
SYQLHNDWRSCAIDLAAAALGAIVAPFPPGRGRLDIQSLLRRCDARAIIVEREYGKTDLCELIESIRPTLLSLRILVVDG
AAGDGWHALDELFRPASIEPDLPTVCPDSPARFLISSGTESEPKWVAYSHNALAGGRGRFLQRIHPEGKTFRGLYLMPLG
TAFGSTATFGVLSWLGGSLIVLRQFDVAAAIQALAELKPTHILGVPTMFQRIAADPALTQADTSSLVAIISGGAKIDETS
IRRCTKAFRCGFISLYGSADGVNCHTTLDDDLETIIRTAGRPNPEICSIRIIDDQKQEVPQGCIGEIAARGPISPMQYVN
DPDLDALYRDQEGWVYTGDLGLIDEEGHLVLSGRKKDIIIRGGVNISPAQIENIAVSHPAVVSAACVPVPDADLGHRVCL
CLVTREGAERPSLSQFTRFLHEKGLETSKLPEYLRYYRQLPLSPAGKIDKKRLTTEIEFTEHPAHRSHPEWAH

Sequences:

>Translated_553_residues
MNHSGILDMVPAEVRAQWARQGIYPNKSLYELFCERVEQQPDNPAVISLDHTTSYAALLDKVHRLATSFQELGIVAGDVI
SYQLHNDWRSCAIDLAAAALGAIVAPFPPGRGRLDIQSLLRRCDARAIIVEREYGKTDLCELIESIRPTLLSLRILVVDG
AAGDGWHALDELFRPASIEPDLPTVCPDSPARFLISSGTESEPKWVAYSHNALAGGRGRFLQRIHPEGKTFRGLYLMPLG
TAFGSTATFGVLSWLGGSLIVLRQFDVAAAIQALAELKPTHILGVPTMFQRIAADPALTQADTSSLVAIISGGAKIDETS
IRRCTKAFRCGFISLYGSADGVNCHTTLDDDLETIIRTAGRPNPEICSIRIIDDQKQEVPQGCIGEIAARGPISPMQYVN
DPDLDALYRDQEGWVYTGDLGLIDEEGHLVLSGRKKDIIIRGGVNISPAQIENIAVSHPAVVSAACVPVPDADLGHRVCL
CLVTREGAERPSLSQFTRFLHEKGLETSKLPEYLRYYRQLPLSPAGKIDKKRLTTEIEFTEHPAHRSHPEWAH
>Mature_553_residues
MNHSGILDMVPAEVRAQWARQGIYPNKSLYELFCERVEQQPDNPAVISLDHTTSYAALLDKVHRLATSFQELGIVAGDVI
SYQLHNDWRSCAIDLAAAALGAIVAPFPPGRGRLDIQSLLRRCDARAIIVEREYGKTDLCELIESIRPTLLSLRILVVDG
AAGDGWHALDELFRPASIEPDLPTVCPDSPARFLISSGTESEPKWVAYSHNALAGGRGRFLQRIHPEGKTFRGLYLMPLG
TAFGSTATFGVLSWLGGSLIVLRQFDVAAAIQALAELKPTHILGVPTMFQRIAADPALTQADTSSLVAIISGGAKIDETS
IRRCTKAFRCGFISLYGSADGVNCHTTLDDDLETIIRTAGRPNPEICSIRIIDDQKQEVPQGCIGEIAARGPISPMQYVN
DPDLDALYRDQEGWVYTGDLGLIDEEGHLVLSGRKKDIIIRGGVNISPAQIENIAVSHPAVVSAACVPVPDADLGHRVCL
CLVTREGAERPSLSQFTRFLHEKGLETSKLPEYLRYYRQLPLSPAGKIDKKRLTTEIEFTEHPAHRSHPEWAH

Specific function: Catalyzes the esterification, concomitant with transport, of exogenous fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids. It has a low activity on medium or long chain fatty acids and is ma

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Membrane; Peripheral membrane protein. Note=Partially membrane-associated [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=531, Percent_Identity=25.0470809792844, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI115511026, Length=504, Percent_Identity=26.3888888888889, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI38505220, Length=525, Percent_Identity=23.2380952380952, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI122937307, Length=546, Percent_Identity=24.1758241758242, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI42544132, Length=393, Percent_Identity=23.6641221374046, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI157311624, Length=480, Percent_Identity=24.375, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI157311622, Length=480, Percent_Identity=24.375, Blast_Score=97, Evalue=4e-20,
Organism=Homo sapiens, GI58082049, Length=453, Percent_Identity=24.7240618101545, Blast_Score=93, Evalue=8e-19,
Organism=Homo sapiens, GI187761345, Length=538, Percent_Identity=22.1189591078067, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI187761343, Length=538, Percent_Identity=22.1189591078067, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI195947378, Length=359, Percent_Identity=24.5125348189415, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI13375727, Length=547, Percent_Identity=23.400365630713, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI145693145, Length=530, Percent_Identity=30.188679245283, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1786810, Length=529, Percent_Identity=27.7882797731569, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1788107, Length=565, Percent_Identity=24.9557522123894, Blast_Score=145, Evalue=9e-36,
Organism=Escherichia coli, GI221142682, Length=502, Percent_Identity=25.4980079681275, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI1788595, Length=431, Percent_Identity=25.2900232018561, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71994703, Length=530, Percent_Identity=24.5283018867925, Blast_Score=135, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI71994694, Length=530, Percent_Identity=24.5283018867925, Blast_Score=135, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI71994690, Length=530, Percent_Identity=24.5283018867925, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI17560308, Length=539, Percent_Identity=23.5621521335807, Blast_Score=131, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI17538037, Length=531, Percent_Identity=24.6704331450094, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17531443, Length=505, Percent_Identity=22.5742574257426, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI17557194, Length=534, Percent_Identity=23.4082397003745, Blast_Score=108, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI32563687, Length=503, Percent_Identity=22.0675944333996, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17559526, Length=518, Percent_Identity=23.7451737451737, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71996755, Length=519, Percent_Identity=23.8921001926782, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17558820, Length=353, Percent_Identity=25.4957507082153, Blast_Score=88, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17560140, Length=478, Percent_Identity=21.7573221757322, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI71985884, Length=371, Percent_Identity=25.0673854447439, Blast_Score=72, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=518, Percent_Identity=23.5521235521236, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24581924, Length=513, Percent_Identity=23.9766081871345, Blast_Score=135, Evalue=8e-32,
Organism=Drosophila melanogaster, GI18859661, Length=349, Percent_Identity=24.9283667621776, Blast_Score=87, Evalue=3e-17,
Organism=Drosophila melanogaster, GI21355181, Length=529, Percent_Identity=20.4158790170132, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI19922652, Length=516, Percent_Identity=21.7054263565891, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI21356947, Length=524, Percent_Identity=23.2824427480916, Blast_Score=86, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24648253, Length=482, Percent_Identity=23.8589211618257, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI281365686, Length=333, Percent_Identity=23.7237237237237, Blast_Score=82, Evalue=8e-16,
Organism=Drosophila melanogaster, GI24648255, Length=482, Percent_Identity=23.6514522821577, Blast_Score=80, Evalue=5e-15,
Organism=Drosophila melanogaster, GI20130357, Length=370, Percent_Identity=24.3243243243243, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24656500, Length=513, Percent_Identity=22.2222222222222, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI21356441, Length=330, Percent_Identity=24.8484848484848, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21358303, Length=319, Percent_Identity=23.1974921630094, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24653035, Length=380, Percent_Identity=23.1578947368421, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI161076582, Length=384, Percent_Identity=22.65625, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24648260, Length=307, Percent_Identity=24.7557003257329, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: NA

Molecular weight: Translated: 60583; Mature: 60583

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHSGILDMVPAEVRAQWARQGIYPNKSLYELFCERVEQQPDNPAVISLDHTTSYAALLD
CCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHH
KVHRLATSFQELGIVAGDVISYQLHNDWRSCAIDLAAAALGAIVAPFPPGRGRLDIQSLL
HHHHHHHHHHHHCCCHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
RRCDARAIIVEREYGKTDLCELIESIRPTLLSLRILVVDGAAGDGWHALDELFRPASIEP
HHCCCEEEEEECCCCCHHHHHHHHHHCHHHHEEEEEEEECCCCCCHHHHHHHCCCCCCCC
DLPTVCPDSPARFLISSGTESEPKWVAYSHNALAGGRGRFLQRIHPEGKTFRGLYLMPLG
CCCCCCCCCCCCEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEEEEC
TAFGSTATFGVLSWLGGSLIVLRQFDVAAAIQALAELKPTHILGVPTMFQRIAADPALTQ
CCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCEEECCHHHHHHHHCCCCCCC
ADTSSLVAIISGGAKIDETSIRRCTKAFRCGFISLYGSADGVNCHTTLDDDLETIIRTAG
CCCCCEEEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCCCCEEECCCCHHHHHHHHHCC
RPNPEICSIRIIDDQKQEVPQGCIGEIAARGPISPMQYVNDPDLDALYRDQEGWVYTGDL
CCCCCEEEEEEECCCHHHCCHHHHHHHHHCCCCCHHHHCCCCCCHHHEECCCCEEEECCE
GLIDEEGHLVLSGRKKDIIIRGGVNISPAQIENIAVSHPAVVSAACVPVPDADLGHRVCL
EEEECCCCEEEECCCCEEEEECCCCCCHHHHHHEECCCCHHHHEEECCCCCCCCCCEEEE
CLVTREGAERPSLSQFTRFLHEKGLETSKLPEYLRYYRQLPLSPAGKIDKKRLTTEIEFT
EEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCEEEEEEC
EHPAHRSHPEWAH
CCCCCCCCCCCCC
>Mature Secondary Structure
MNHSGILDMVPAEVRAQWARQGIYPNKSLYELFCERVEQQPDNPAVISLDHTTSYAALLD
CCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHH
KVHRLATSFQELGIVAGDVISYQLHNDWRSCAIDLAAAALGAIVAPFPPGRGRLDIQSLL
HHHHHHHHHHHHCCCHHHHEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
RRCDARAIIVEREYGKTDLCELIESIRPTLLSLRILVVDGAAGDGWHALDELFRPASIEP
HHCCCEEEEEECCCCCHHHHHHHHHHCHHHHEEEEEEEECCCCCCHHHHHHHCCCCCCCC
DLPTVCPDSPARFLISSGTESEPKWVAYSHNALAGGRGRFLQRIHPEGKTFRGLYLMPLG
CCCCCCCCCCCCEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCEEEEEEC
TAFGSTATFGVLSWLGGSLIVLRQFDVAAAIQALAELKPTHILGVPTMFQRIAADPALTQ
CCCCCHHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCEEECCHHHHHHHHCCCCCCC
ADTSSLVAIISGGAKIDETSIRRCTKAFRCGFISLYGSADGVNCHTTLDDDLETIIRTAG
CCCCCEEEEEECCCCCCHHHHHHHHHHHHCCEEEEECCCCCCEEECCCCHHHHHHHHHCC
RPNPEICSIRIIDDQKQEVPQGCIGEIAARGPISPMQYVNDPDLDALYRDQEGWVYTGDL
CCCCCEEEEEEECCCHHHCCHHHHHHHHHCCCCCHHHHCCCCCCHHHEECCCCEEEECCE
GLIDEEGHLVLSGRKKDIIIRGGVNISPAQIENIAVSHPAVVSAACVPVPDADLGHRVCL
EEEECCCCEEEECCCCEEEEECCCCCCHHHHHHEECCCCHHHHEEECCCCCCCCCCEEEE
CLVTREGAERPSLSQFTRFLHEKGLETSKLPEYLRYYRQLPLSPAGKIDKKRLTTEIEFT
EEEECCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCHHHCEEEEEEC
EHPAHRSHPEWAH
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 7984428; 12535077 [H]