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The map label for this gene is ccsA [H]

Identifier: 82701987

GI number: 82701987

Start: 977411

End: 978712

Strand: Reverse

Name: ccsA [H]

Synonym: Nmul_A0858

Alternate gene names: 82701987

Gene position: 978712-977411 (Counterclockwise)

Preceding gene: 82701988

Following gene: 82701986

Centisome position: 30.74

GC content: 55.53

Gene sequence:

>1302_bases
ATGGAACATAAACGTAACGCAATCCTTGATCTGGGTAACGGTGAGCCGCCACTTGAATTTCCGATCCTGGCAGGCACCAT
GGGGCCTGAAGTTGTTGACATCCGCACGCTTTATAAGAGGGGCCGCGTATTCACCTACGATCCAGGCTTCATGTCCACGG
CCAGCAGCAGTTCCAGCATAACTTTTATCGATGGCGACAAGGGAATTCTGCTCTATCGCGGCTACCCCATCGAACAGTTG
GCGAAGCAGTGCGATTTCATGGAAGTATGCTACCTGCTGATGAATGGAGAGCTGCCGAATATCGCCCAGAAGGCGTCGTT
CGACAAAACCATCAAAGACCACACGATGGTGCATGAACAACTGGCGCGCTTTTATACCGGTTTCAGGCGGGACGCGCATC
CCATGGCGGTCATGGTAGGGGTGGTGGGAGCCTTGTCCGCCTTTTATCACGACGCAATGGACATTTCCGACGCGGCTCAT
CGTGAACTCTCCGCCGTCCGCCTGGTCGCGAAGCTGCCGACCATTACTGCCATGGCCTACAAGTACAATATCGGCCAGCC
GTTCATGTATCCCCGCAACAATCTCAATTATGCGGAAAACTTCCTGCACATGATGTTTGCCACTCCGGCGGAGGAGTATC
GATCGAATCCGGTACTTGCACGCGCGCTCGACCGTATCCTGATCCTGCATGCCGACCATGAGCAGAATGCCTCCACCTCT
ACAGTACGCCTCGCCGGTTCTACCGGCGCCAACCCGTTTGCGTGTATTTCCGCCGGGATTGCCTGCCTGTGGGGGCCCGC
GCATGGCGGAGCGAACGAAGCCTGCCTGAACATGCTGGAAGAGATCGGCGATGAATCGCGCATTGGCGAATACATTGCCC
GCGCCAAAGACCCGGATGATCCTTTCCGGCTGATGGGATTCGGCCATCGGGTATACAGGAATTTCGATCCGCGCGCGACG
CTGATGCGCGAAACCTGCCACGAAGTCCTGAATGAACTGGGTTTGCGCGACGATCGTTTATTCAAGCTTGCGCTCAAGCT
GGAGAAAATCGCGCTCGAAGATGAATATTTCATTTCCAAAAAACTTTATCCCAACGTCGATTTTTACTCCGGCATCGTGC
AGAGGGCGCTGGGTATTCCCGTTTCCATGTTCACCGCGATATTCACCATGGCCCGCACTGTCGGCTGGGTCGCCCAGTGG
AGCGAAATGATCGCAGACCCGGAATACAAGATCGGCCGTCCACGCCAGCTTTATACTGGCGCAGCCACACGCGATGTCAT
TCCCATCGCATCCCGGTTGTAA

Upstream 100 bases:

>100_bases
AAGGAAAGGCAGATCTGCAGCCGGTATTGCGCCTGCTGCAATCAGTTTAGCAAGGAATCTCCTGGGTAAATCAAGATAAC
GGAAAACAGTGGGAAACGAC

Downstream 100 bases:

>100_bases
GCGGACCGTCAAAAGACGAGGTTTCAGGCCATGCTGAAACAGTTACACGACGATTCTCCACTGTTCGGATCCAATGCCTC
CTTCGTGGAGGGGCTTTATG

Product: citrate synthase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MEHKRNAILDLGNGEPPLEFPILAGTMGPEVVDIRTLYKRGRVFTYDPGFMSTASSSSSITFIDGDKGILLYRGYPIEQL
AKQCDFMEVCYLLMNGELPNIAQKASFDKTIKDHTMVHEQLARFYTGFRRDAHPMAVMVGVVGALSAFYHDAMDISDAAH
RELSAVRLVAKLPTITAMAYKYNIGQPFMYPRNNLNYAENFLHMMFATPAEEYRSNPVLARALDRILILHADHEQNASTS
TVRLAGSTGANPFACISAGIACLWGPAHGGANEACLNMLEEIGDESRIGEYIARAKDPDDPFRLMGFGHRVYRNFDPRAT
LMRETCHEVLNELGLRDDRLFKLALKLEKIALEDEYFISKKLYPNVDFYSGIVQRALGIPVSMFTAIFTMARTVGWVAQW
SEMIADPEYKIGRPRQLYTGAATRDVIPIASRL

Sequences:

>Translated_433_residues
MEHKRNAILDLGNGEPPLEFPILAGTMGPEVVDIRTLYKRGRVFTYDPGFMSTASSSSSITFIDGDKGILLYRGYPIEQL
AKQCDFMEVCYLLMNGELPNIAQKASFDKTIKDHTMVHEQLARFYTGFRRDAHPMAVMVGVVGALSAFYHDAMDISDAAH
RELSAVRLVAKLPTITAMAYKYNIGQPFMYPRNNLNYAENFLHMMFATPAEEYRSNPVLARALDRILILHADHEQNASTS
TVRLAGSTGANPFACISAGIACLWGPAHGGANEACLNMLEEIGDESRIGEYIARAKDPDDPFRLMGFGHRVYRNFDPRAT
LMRETCHEVLNELGLRDDRLFKLALKLEKIALEDEYFISKKLYPNVDFYSGIVQRALGIPVSMFTAIFTMARTVGWVAQW
SEMIADPEYKIGRPRQLYTGAATRDVIPIASRL
>Mature_433_residues
MEHKRNAILDLGNGEPPLEFPILAGTMGPEVVDIRTLYKRGRVFTYDPGFMSTASSSSSITFIDGDKGILLYRGYPIEQL
AKQCDFMEVCYLLMNGELPNIAQKASFDKTIKDHTMVHEQLARFYTGFRRDAHPMAVMVGVVGALSAFYHDAMDISDAAH
RELSAVRLVAKLPTITAMAYKYNIGQPFMYPRNNLNYAENFLHMMFATPAEEYRSNPVLARALDRILILHADHEQNASTS
TVRLAGSTGANPFACISAGIACLWGPAHGGANEACLNMLEEIGDESRIGEYIARAKDPDDPFRLMGFGHRVYRNFDPRAT
LMRETCHEVLNELGLRDDRLFKLALKLEKIALEDEYFISKKLYPNVDFYSGIVQRALGIPVSMFTAIFTMARTVGWVAQW
SEMIADPEYKIGRPRQLYTGAATRDVIPIASRL

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=391, Percent_Identity=27.8772378516624, Blast_Score=116, Evalue=4e-26,
Organism=Escherichia coli, GI1786939, Length=413, Percent_Identity=66.3438256658596, Blast_Score=593, Evalue=1e-171,
Organism=Escherichia coli, GI1786527, Length=367, Percent_Identity=31.0626702997275, Blast_Score=156, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17555174, Length=402, Percent_Identity=28.3582089552239, Blast_Score=123, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324328, Length=404, Percent_Identity=26.7326732673267, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319850, Length=364, Percent_Identity=26.9230769230769, Blast_Score=103, Evalue=7e-23,
Organism=Saccharomyces cerevisiae, GI6325257, Length=411, Percent_Identity=27.2506082725061, Blast_Score=103, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24640126, Length=393, Percent_Identity=26.7175572519084, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24640124, Length=393, Percent_Identity=27.2264631043257, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI21356863, Length=394, Percent_Identity=27.4111675126904, Blast_Score=96, Evalue=4e-20,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 48532; Mature: 48532

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHKRNAILDLGNGEPPLEFPILAGTMGPEVVDIRTLYKRGRVFTYDPGFMSTASSSSSI
CCCCCCCEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCEE
TFIDGDKGILLYRGYPIEQLAKQCDFMEVCYLLMNGELPNIAQKASFDKTIKDHTMVHEQ
EEEECCCCEEEECCCCHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LARFYTGFRRDAHPMAVMVGVVGALSAFYHDAMDISDAAHRELSAVRLVAKLPTITAMAY
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEE
KYNIGQPFMYPRNNLNYAENFLHMMFATPAEEYRSNPVLARALDRILILHADHEQNASTS
EECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCHHHHHHHHEEEEECCCCCCCCCC
TVRLAGSTGANPFACISAGIACLWGPAHGGANEACLNMLEEIGDESRIGEYIARAKDPDD
EEEEECCCCCCHHHHHHHCHHEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCC
PFRLMGFGHRVYRNFDPRATLMRETCHEVLNELGLRDDRLFKLALKLEKIALEDEYFISK
CHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHH
KLYPNVDFYSGIVQRALGIPVSMFTAIFTMARTVGWVAQWSEMIADPEYKIGRPRQLYTG
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECC
AATRDVIPIASRL
CCCCCCCHHHHCC
>Mature Secondary Structure
MEHKRNAILDLGNGEPPLEFPILAGTMGPEVVDIRTLYKRGRVFTYDPGFMSTASSSSSI
CCCCCCCEEECCCCCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEECCCCCCCCCCCCEE
TFIDGDKGILLYRGYPIEQLAKQCDFMEVCYLLMNGELPNIAQKASFDKTIKDHTMVHEQ
EEEECCCCEEEECCCCHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
LARFYTGFRRDAHPMAVMVGVVGALSAFYHDAMDISDAAHRELSAVRLVAKLPTITAMAY
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEE
KYNIGQPFMYPRNNLNYAENFLHMMFATPAEEYRSNPVLARALDRILILHADHEQNASTS
EECCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCHHHHHHHHEEEEECCCCCCCCCC
TVRLAGSTGANPFACISAGIACLWGPAHGGANEACLNMLEEIGDESRIGEYIARAKDPDD
EEEEECCCCCCHHHHHHHCHHEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCC
PFRLMGFGHRVYRNFDPRATLMRETCHEVLNELGLRDDRLFKLALKLEKIALEDEYFISK
CHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHH
KLYPNVDFYSGIVQRALGIPVSMFTAIFTMARTVGWVAQWSEMIADPEYKIGRPRQLYTG
CCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECC
AATRDVIPIASRL
CCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8526514 [H]