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The map label for this gene is gluQ [H]

Identifier: 82701930

GI number: 82701930

Start: 911952

End: 912863

Strand: Reverse

Name: gluQ [H]

Synonym: Nmul_A0797

Alternate gene names: 82701930

Gene position: 912863-911952 (Counterclockwise)

Preceding gene: 82701934

Following gene: 82701929

Centisome position: 28.67

GC content: 50.44

Gene sequence:

>912_bases
GTGGAAAATGCCTTCCCTCTCTCGCCGGTCTATCGCGGCCGGTTTGCCCCCTCTCCTACCGGACCCTTGCATTTTGGTTC
TCTTGTCACTGCTGTAGGCAGTTATCTGGAGGCAAGATCACGGGGCGGAGAATGGCTCGTCAGAATCGAAAATCTCGATA
CGACGCGTGAAATTCCAGGTGCTTCAGAAGAAATTTTGAATACCCTGCATCTCCTTGGAATGGAATGGGATGGCTCAGTG
GTATTTCAGGAGCAACGCCAGGAGGTCTACCATGCAGCTTTGTCGATCCTTGAAAAACAGCAGCTTATTTATCGTTGCAG
TTGCAGTCGTAAGGAAATTGCCGATTCCTCAGTTCTTGGAATAGAAGGGCCTGTCTATCCTGGAACTTGCCGCGGCCGCA
AACATGAACCCCCATTTTCCGGTGCATGGCGACTATATACAAATAATGACTTGATTGAATTTGAGGATGAAGTACAAGGC
CTGCGGCAACAGCGGATTCAGCACGATATAGGCGATTTCGTGATATGTCGGGCGGATGGGATTTTCGCTTACCAGCTAGC
GGTCGTGGTGGATGACGCTGAACAGGGTATTACTCACGTGGTGCGGGGAGGTGATTTACTGAACTCGACTCCCCGTCAGA
TTTACCTGCAAAAATTGCTGGGCTACTCCATTCCCTCCTATATGCACCTTCCCGTAGTAGTCAACGCACGAGGCGAAAAA
CTCAGTAAACAGACGCAGGCAGCGCCGGTAGATATTTCGTATCCCGTTTTGCAGCTCGTGAAGGCCATCCGGTTTCTCGG
GCAGACACCGCCGCCGGAGGTAAGCGAGAGCAGCTTGAGGTCATTCTGGATATGGGCAATTAAAAATTGGGATCGGCAGG
CAATTCCAAAAAACATGTCACTCATGTTTTAA

Upstream 100 bases:

>100_bases
GTATACTCATCATGTTACTCCCTCTGGATAGAGGGGTTGGAGAATAAGAATCTGAGGGGTTCAATGGATATTGTGAGACT
CCATTAATCAAGATTAACGG

Downstream 100 bases:

>100_bases
CCTGATCAATCTGCCGGAGTGCTAATAGATGAAATGAAGACCGTGCTACTTGCCACCAAGCTGCCAGCACTCCGGTTTAG
AAATGAGTTTATACTGAAAA

Product: glutamate--tRNA ligase

Products: AMP; diphosphate; L-glutamyl-tRNA(Glu)

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MENAFPLSPVYRGRFAPSPTGPLHFGSLVTAVGSYLEARSRGGEWLVRIENLDTTREIPGASEEILNTLHLLGMEWDGSV
VFQEQRQEVYHAALSILEKQQLIYRCSCSRKEIADSSVLGIEGPVYPGTCRGRKHEPPFSGAWRLYTNNDLIEFEDEVQG
LRQQRIQHDIGDFVICRADGIFAYQLAVVVDDAEQGITHVVRGGDLLNSTPRQIYLQKLLGYSIPSYMHLPVVVNARGEK
LSKQTQAAPVDISYPVLQLVKAIRFLGQTPPPEVSESSLRSFWIWAIKNWDRQAIPKNMSLMF

Sequences:

>Translated_303_residues
MENAFPLSPVYRGRFAPSPTGPLHFGSLVTAVGSYLEARSRGGEWLVRIENLDTTREIPGASEEILNTLHLLGMEWDGSV
VFQEQRQEVYHAALSILEKQQLIYRCSCSRKEIADSSVLGIEGPVYPGTCRGRKHEPPFSGAWRLYTNNDLIEFEDEVQG
LRQQRIQHDIGDFVICRADGIFAYQLAVVVDDAEQGITHVVRGGDLLNSTPRQIYLQKLLGYSIPSYMHLPVVVNARGEK
LSKQTQAAPVDISYPVLQLVKAIRFLGQTPPPEVSESSLRSFWIWAIKNWDRQAIPKNMSLMF
>Mature_303_residues
MENAFPLSPVYRGRFAPSPTGPLHFGSLVTAVGSYLEARSRGGEWLVRIENLDTTREIPGASEEILNTLHLLGMEWDGSV
VFQEQRQEVYHAALSILEKQQLIYRCSCSRKEIADSSVLGIEGPVYPGTCRGRKHEPPFSGAWRLYTNNDLIEFEDEVQG
LRQQRIQHDIGDFVICRADGIFAYQLAVVVDDAEQGITHVVRGGDLLNSTPRQIYLQKLLGYSIPSYMHLPVVVNARGEK
LSKQTQAAPVDISYPVLQLVKAIRFLGQTPPPEVSESSLRSFWIWAIKNWDRQAIPKNMSLMF

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=251, Percent_Identity=37.0517928286853, Blast_Score=139, Evalue=2e-33,
Organism=Escherichia coli, GI226510925, Length=288, Percent_Identity=44.7916666666667, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1788743, Length=245, Percent_Identity=36.3265306122449, Blast_Score=154, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI71993394, Length=259, Percent_Identity=33.2046332046332, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324540, Length=310, Percent_Identity=31.9354838709677, Blast_Score=141, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24665321, Length=253, Percent_Identity=35.5731225296443, Blast_Score=141, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: 6.1.1.17

Molecular weight: Translated: 34113; Mature: 34113

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENAFPLSPVYRGRFAPSPTGPLHFGSLVTAVGSYLEARSRGGEWLVRIENLDTTREIPG
CCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
ASEEILNTLHLLGMEWDGSVVFQEQRQEVYHAALSILEKQQLIYRCSCSRKEIADSSVLG
CHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEC
IEGPVYPGTCRGRKHEPPFSGAWRLYTNNDLIEFEDEVQGLRQQRIQHDIGDFVICRADG
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHCCCCEEEEECCC
IFAYQLAVVVDDAEQGITHVVRGGDLLNSTPRQIYLQKLLGYSIPSYMHLPVVVNARGEK
EEEEEEEEEEECHHHHHHHHHCCCHHHCCCHHHHHHHHHHCCCCCCCEECCEEEECCCCH
LSKQTQAAPVDISYPVLQLVKAIRFLGQTPPPEVSESSLRSFWIWAIKNWDRQAIPKNMS
HHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHEEEEEECCCCHHHCCCCCC
LMF
CCC
>Mature Secondary Structure
MENAFPLSPVYRGRFAPSPTGPLHFGSLVTAVGSYLEARSRGGEWLVRIENLDTTREIPG
CCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCC
ASEEILNTLHLLGMEWDGSVVFQEQRQEVYHAALSILEKQQLIYRCSCSRKEIADSSVLG
CHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCEEC
IEGPVYPGTCRGRKHEPPFSGAWRLYTNNDLIEFEDEVQGLRQQRIQHDIGDFVICRADG
CCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEEHHHHHHHHHHHHHHCCCCEEEEECCC
IFAYQLAVVVDDAEQGITHVVRGGDLLNSTPRQIYLQKLLGYSIPSYMHLPVVVNARGEK
EEEEEEEEEEECHHHHHHHHHCCCHHHCCCHHHHHHHHHHCCCCCCCEECCEEEECCCCH
LSKQTQAAPVDISYPVLQLVKAIRFLGQTPPPEVSESSLRSFWIWAIKNWDRQAIPKNMS
HHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHEEEEEECCCCHHHCCCCCC
LMF
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; tRNA(Glu)

Specific reaction: ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA