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The map label for this gene is nifS [H]

Identifier: 82701808

GI number: 82701808

Start: 773738

End: 774895

Strand: Direct

Name: nifS [H]

Synonym: Nmul_A0675

Alternate gene names: 82701808

Gene position: 773738-774895 (Clockwise)

Preceding gene: 82701807

Following gene: 82701809

Centisome position: 24.3

GC content: 59.76

Gene sequence:

>1158_bases
ATGACCTACGCCTATTTCGACCATAACGCCACGACTGCTGTCGATAAAGCCGCGCTTGATGCCATGTTGCCGTATTTCCA
GGAAGAGTACGGCAATCCCTCCAGCCGGCATGCTCCTGGCATTGCAGCTCGCCGGGCGGTTGATCGCGCCCGTGAGCAAG
TGGCGGATGCAGTGGGAGTGCAGCCGTCGCAGGTGGTTTTCACCAGCGGGGGATCGGAAGCCAATAACCTCTTCATTCAG
GGCGCCGCGGGCTACATGAAGCCGGGACAGATTGCGATAAGCGCGATCGAGCATCCCTGCGTGATGAAAACTGCGCAGGA
ATTGGCGCGTGCTTCGCGGGGAAGCTGGAATCTGCGTCGGCTAGCAGTGGATAATCAGGGCCGCCTGGACCCGGCCGCTC
TTGACGAGGCACTGTCGTCTCAACAGTCGGGGCTGGTTTCAGTCATGCTGGCAAACAACGAAACAGGCGTGATTCAGGAT
GTTTCGATGGTGGGGGAGAAGGCCCGCGCGCGAGGTGCCTGGATTCACACGGACGCAGTGCAGGCGTTTGGCAAGATACC
TGTCGATTTTGCCTCGCTCAATGTACATGCCCTGACGCTGTCGTCCCACAAGATTTGCGGTCCCAAGGGGGCCGCGGCGC
TTATCATGGATAAGCGGCTCCTGCTCAAACCTCTCATCTACGGTGGGGGGCATGAAGGCGGATTGCGTTCGGGTACCGAA
AACGTTCCCGCAATTGTGGGATTCGGCGTGGCCTGCGAGTTGGTCAAGAGACGAATAGCGGAACATACCACGCATATCTC
GGCATTGCGCCAGCAACTGGAGCGGGGCCTGCTGGAGATGGGCGGGACTGTTTTTGGTCTCGGCGCTTCCCGTCTGCCCA
ATACCTGCTATTTTGCGCTCCCGGGTATCGAAGGAGATACTTTGGTAGTACGTCTCGATAAAGCGGGTTTCGCGGTGGCA
AGCGGCGCGGCGTGTTCCAGCGTCAACCCCGGGCGAAGCCATGTGCTGGAAGCAATGGGGGTGGATCCTGCCCTGGCTCG
TTGCGCTGTGCGGGTAAGCCTGGGGGCCTCCAATTCTTCGGTGCAGGTTGCTGATTTCCTTCGCGCTCTCGGTGCTATCG
TGGGGGAGCTGGAGCAGATGAGCGCGCTTTCGATCTGA

Upstream 100 bases:

>100_bases
CTGCTCCTCATCGCAACATCGTACAGTTCCTGCTTGAACGGTAACGCCTGCTGGTTTCAAGTCCGATTTTCCATTTCGAC
CAGTTTGAAGGATCCGGACG

Downstream 100 bases:

>100_bases
CTGTTTCCCTTGATACCCGTGAGGAGAGAGCCCTTGCAGGGTTAGTAGGGGTTAAAGAGGCTATAAAGATCGTCTGCATT
TGAGGGACTGGAATGCACCG

Product: class V aminotransferase

Products: NA

Alternate protein names: Nitrogenase metalloclusters biosynthesis protein NifS [H]

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MTYAYFDHNATTAVDKAALDAMLPYFQEEYGNPSSRHAPGIAARRAVDRAREQVADAVGVQPSQVVFTSGGSEANNLFIQ
GAAGYMKPGQIAISAIEHPCVMKTAQELARASRGSWNLRRLAVDNQGRLDPAALDEALSSQQSGLVSVMLANNETGVIQD
VSMVGEKARARGAWIHTDAVQAFGKIPVDFASLNVHALTLSSHKICGPKGAAALIMDKRLLLKPLIYGGGHEGGLRSGTE
NVPAIVGFGVACELVKRRIAEHTTHISALRQQLERGLLEMGGTVFGLGASRLPNTCYFALPGIEGDTLVVRLDKAGFAVA
SGAACSSVNPGRSHVLEAMGVDPALARCAVRVSLGASNSSVQVADFLRALGAIVGELEQMSALSI

Sequences:

>Translated_385_residues
MTYAYFDHNATTAVDKAALDAMLPYFQEEYGNPSSRHAPGIAARRAVDRAREQVADAVGVQPSQVVFTSGGSEANNLFIQ
GAAGYMKPGQIAISAIEHPCVMKTAQELARASRGSWNLRRLAVDNQGRLDPAALDEALSSQQSGLVSVMLANNETGVIQD
VSMVGEKARARGAWIHTDAVQAFGKIPVDFASLNVHALTLSSHKICGPKGAAALIMDKRLLLKPLIYGGGHEGGLRSGTE
NVPAIVGFGVACELVKRRIAEHTTHISALRQQLERGLLEMGGTVFGLGASRLPNTCYFALPGIEGDTLVVRLDKAGFAVA
SGAACSSVNPGRSHVLEAMGVDPALARCAVRVSLGASNSSVQVADFLRALGAIVGELEQMSALSI
>Mature_384_residues
TYAYFDHNATTAVDKAALDAMLPYFQEEYGNPSSRHAPGIAARRAVDRAREQVADAVGVQPSQVVFTSGGSEANNLFIQG
AAGYMKPGQIAISAIEHPCVMKTAQELARASRGSWNLRRLAVDNQGRLDPAALDEALSSQQSGLVSVMLANNETGVIQDV
SMVGEKARARGAWIHTDAVQAFGKIPVDFASLNVHALTLSSHKICGPKGAAALIMDKRLLLKPLIYGGGHEGGLRSGTEN
VPAIVGFGVACELVKRRIAEHTTHISALRQQLERGLLEMGGTVFGLGASRLPNTCYFALPGIEGDTLVVRLDKAGFAVAS
GAACSSVNPGRSHVLEAMGVDPALARCAVRVSLGASNSSVQVADFLRALGAIVGELEQMSALSI

Specific function: Catalyzes the removal of elemental sulfur atoms from cysteine to produce alanine. Seems to participate in the biosynthesis of the nitrogenase metalloclusters by providing the inorganic sulfur required for the Fe-S core formation [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=386, Percent_Identity=35.4922279792746, Blast_Score=228, Evalue=5e-60,
Organism=Homo sapiens, GI156713448, Length=415, Percent_Identity=32.289156626506, Blast_Score=182, Evalue=5e-46,
Organism=Escherichia coli, GI48994898, Length=387, Percent_Identity=37.4677002583979, Blast_Score=236, Evalue=1e-63,
Organism=Escherichia coli, GI1789175, Length=348, Percent_Identity=25.2873563218391, Blast_Score=63, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI25143064, Length=367, Percent_Identity=35.4223433242507, Blast_Score=228, Evalue=5e-60,
Organism=Caenorhabditis elegans, GI17533177, Length=332, Percent_Identity=32.5301204819277, Blast_Score=147, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6319831, Length=385, Percent_Identity=36.8831168831169, Blast_Score=242, Evalue=9e-65,
Organism=Drosophila melanogaster, GI20129463, Length=369, Percent_Identity=37.6693766937669, Blast_Score=228, Evalue=6e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 40426; Mature: 40294

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTYAYFDHNATTAVDKAALDAMLPYFQEEYGNPSSRHAPGIAARRAVDRAREQVADAVGV
CEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
QPSQVVFTSGGSEANNLFIQGAAGYMKPGQIAISAIEHPCVMKTAQELARASRGSWNLRR
CCCEEEEECCCCCCCEEEEEECCCCCCCCCEEEEHHCCCHHHHHHHHHHHHCCCCCCEEE
LAVDNQGRLDPAALDEALSSQQSGLVSVMLANNETGVIQDVSMVGEKARARGAWIHTDAV
EEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEHHHH
QAFGKIPVDFASLNVHALTLSSHKICGPKGAAALIMDKRLLLKPLIYGGGHEGGLRSGTE
HHHCCCCCEEEECEEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCC
NVPAIVGFGVACELVKRRIAEHTTHISALRQQLERGLLEMGGTVFGLGASRLPNTCYFAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEE
PGIEGDTLVVRLDKAGFAVASGAACSSVNPGRSHVLEAMGVDPALARCAVRVSLGASNSS
CCCCCCEEEEEECCCCCEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHEEEEEECCCCCC
VQVADFLRALGAIVGELEQMSALSI
CHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TYAYFDHNATTAVDKAALDAMLPYFQEEYGNPSSRHAPGIAARRAVDRAREQVADAVGV
EEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCC
QPSQVVFTSGGSEANNLFIQGAAGYMKPGQIAISAIEHPCVMKTAQELARASRGSWNLRR
CCCEEEEECCCCCCCEEEEEECCCCCCCCCEEEEHHCCCHHHHHHHHHHHHCCCCCCEEE
LAVDNQGRLDPAALDEALSSQQSGLVSVMLANNETGVIQDVSMVGEKARARGAWIHTDAV
EEECCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCEEEHHHH
QAFGKIPVDFASLNVHALTLSSHKICGPKGAAALIMDKRLLLKPLIYGGGHEGGLRSGTE
HHHCCCCCEEEECEEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCCCCCCCCC
NVPAIVGFGVACELVKRRIAEHTTHISALRQQLERGLLEMGGTVFGLGASRLPNTCYFAL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCEEEEE
PGIEGDTLVVRLDKAGFAVASGAACSSVNPGRSHVLEAMGVDPALARCAVRVSLGASNSS
CCCCCCEEEEEECCCCCEEECCCCCCCCCCCHHHHHHHHCCCHHHHHHEEEEEECCCCCC
VQVADFLRALGAIVGELEQMSALSI
CHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11092875 [H]