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The map label for this gene is trmJ [H]

Identifier: 82701806

GI number: 82701806

Start: 772035

End: 772838

Strand: Direct

Name: trmJ [H]

Synonym: Nmul_A0673

Alternate gene names: 82701806

Gene position: 772035-772838 (Clockwise)

Preceding gene: 82701802

Following gene: 82701807

Centisome position: 24.25

GC content: 56.22

Gene sequence:

>804_bases
TTGCACAGGGTAGTCATCTCCAATGGAGTCTTTAATGGGCTATCCGGATTAAATGTGAATCCCCTCCATCTGCTCGATAA
AGTCCGAATTGTCCTGAGCCATACCAGCCATCCCGGTAACATCGGGGGGGCGGCGCGGGCAATGAAGACCATGGGATTGG
ACTCGCTCTATCTTGTGAATCCCCGGTTTTTCCCCGACAAGGAAGCTGAAAGACGAGCCACCGGCGCACTGGATATCCTC
CATAAGGCAAAAGTCTGCGACACGCTCGATGAAGCGCTCAGTGGCACTGTTCTGGCAGCGGCAGTGACGGCCCGACAACG
CGATCTCTCCCATGAAGTTTTTGATGGCCGGCAGGGTGCGACCAAACTGCTCGATCTCGCGCAAAAGGATTTCGTTGCGC
TGGTCTTCGGCACGGAAATGTCCGGACTTACCACGCTTGAAGTCAGTAAGTGCCAGATCATCATCCATATTCCAGCCAAC
CCGGAATATTCATCTCTCAATCTTGCCAGCGCTGTGCAGGTCATGGCATACGAATTGCGCATGGCCTGCAGGGATTTGCT
GGAAACCCCGCCTGCTTCGCCGACCCTGACCCTGCATGGCGGGGGGATGGCGGCCACGTTCGATGAAATCGAGTTGCTTT
ACGCTCACCTGGAGCAGGTGGCGATCAGCAGCGGTTTTCTCGATCCGCAGGAGCCGAAACGCCTGATGCAGCGCATGCGA
CGGCTGTTTGCCCGCGCGCGGCTGGAAAAGGAGGAGGTCAATATATTACGAGGGATACTGAGCGCCTGTGAGAAGCGGAT
GTAG

Upstream 100 bases:

>100_bases
TAATCCATCAGGATGCGGAATGATGCCCGGGCAGGTTGCGAGACAGTGCTATCAATCGGGAAAAATCCCGTAGCCTGAAA
TGGCTACAAACTGACTGCGC

Downstream 100 bases:

>100_bases
ACGGAGGCTGCTTTCCCGCTTATTCCTGATGCTCCGGGATGCGAACGAAATTGAATGATGGCGGGAAGAGTTGACGGCGG
TCAGGTTTAAACGAAAGTAG

Product: RNA methyltransferase TrmH

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 267; Mature: 267

Protein sequence:

>267_residues
MHRVVISNGVFNGLSGLNVNPLHLLDKVRIVLSHTSHPGNIGGAARAMKTMGLDSLYLVNPRFFPDKEAERRATGALDIL
HKAKVCDTLDEALSGTVLAAAVTARQRDLSHEVFDGRQGATKLLDLAQKDFVALVFGTEMSGLTTLEVSKCQIIIHIPAN
PEYSSLNLASAVQVMAYELRMACRDLLETPPASPTLTLHGGGMAATFDEIELLYAHLEQVAISSGFLDPQEPKRLMQRMR
RLFARARLEKEEVNILRGILSACEKRM

Sequences:

>Translated_267_residues
MHRVVISNGVFNGLSGLNVNPLHLLDKVRIVLSHTSHPGNIGGAARAMKTMGLDSLYLVNPRFFPDKEAERRATGALDIL
HKAKVCDTLDEALSGTVLAAAVTARQRDLSHEVFDGRQGATKLLDLAQKDFVALVFGTEMSGLTTLEVSKCQIIIHIPAN
PEYSSLNLASAVQVMAYELRMACRDLLETPPASPTLTLHGGGMAATFDEIELLYAHLEQVAISSGFLDPQEPKRLMQRMR
RLFARARLEKEEVNILRGILSACEKRM
>Mature_267_residues
MHRVVISNGVFNGLSGLNVNPLHLLDKVRIVLSHTSHPGNIGGAARAMKTMGLDSLYLVNPRFFPDKEAERRATGALDIL
HKAKVCDTLDEALSGTVLAAAVTARQRDLSHEVFDGRQGATKLLDLAQKDFVALVFGTEMSGLTTLEVSKCQIIIHIPAN
PEYSSLNLASAVQVMAYELRMACRDLLETPPASPTLTLHGGGMAATFDEIELLYAHLEQVAISSGFLDPQEPKRLMQRMR
RLFARARLEKEEVNILRGILSACEKRM

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=243, Percent_Identity=47.7366255144033, Blast_Score=211, Evalue=6e-56,
Organism=Escherichia coli, GI1790865, Length=158, Percent_Identity=35.4430379746835, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 29298; Mature: 29298

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHRVVISNGVFNGLSGLNVNPLHLLDKVRIVLSHTSHPGNIGGAARAMKTMGLDSLYLVN
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEEEC
PRFFPDKEAERRATGALDILHKAKVCDTLDEALSGTVLAAAVTARQRDLSHEVFDGRQGA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHH
TKLLDLAQKDFVALVFGTEMSGLTTLEVSKCQIIIHIPANPEYSSLNLASAVQVMAYELR
HHHHHHHHHCEEEEEECCCCCCCEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
MACRDLLETPPASPTLTLHGGGMAATFDEIELLYAHLEQVAISSGFLDPQEPKRLMQRMR
HHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
RLFARARLEKEEVNILRGILSACEKRM
HHHHHHHCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHRVVISNGVFNGLSGLNVNPLHLLDKVRIVLSHTSHPGNIGGAARAMKTMGLDSLYLVN
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCEEEEEC
PRFFPDKEAERRATGALDILHKAKVCDTLDEALSGTVLAAAVTARQRDLSHEVFDGRQGA
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHH
TKLLDLAQKDFVALVFGTEMSGLTTLEVSKCQIIIHIPANPEYSSLNLASAVQVMAYELR
HHHHHHHHHCEEEEEECCCCCCCEEEEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHH
MACRDLLETPPASPTLTLHGGGMAATFDEIELLYAHLEQVAISSGFLDPQEPKRLMQRMR
HHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHH
RLFARARLEKEEVNILRGILSACEKRM
HHHHHHHCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA