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The map label for this gene is pth

Identifier: 82701725

GI number: 82701725

Start: 676160

End: 676735

Strand: Direct

Name: pth

Synonym: Nmul_A0591

Alternate gene names: 82701725

Gene position: 676160-676735 (Clockwise)

Preceding gene: 82701724

Following gene: 82701726

Centisome position: 21.23

GC content: 57.81

Gene sequence:

>576_bases
GTGAAGCTCATCGTCGGTCTTGGCAATCCGGGTAGGGAATATGCGGCCACTCGCCATAATGCCGGCGCCTGGTGGGTAGT
TCGTCTGGCGGATCAATTGGGTGTCACGTTGAAGGCGGATGGCAAGTTTCATGGCTTGTGCGCGCGTATCGGGCAGGGTG
AGTCTGAATCCTGGCTGCTCAATCCCCAGACCTATATGAATGCTAGTGGAAGGGCAGTGGCCGCATTGTGCCGTTTCTAC
CGAATTCAGCCTGAACAGATGCTCGTAGTGCACGACGAACTGGATTTGCCCCCCGGCGTCTCCAGGCTGAAGCTCGACGG
AGGGTTGGGCGGTCACAATGGGTTGAAAGACATCGTCGCGCATCTCGGCACCCGGGAGTTCTGGCGCTTGCGGATCGGCA
TCGGCCATCCGGGAGAAAAGCATGCCGTCGTGAATTACGTACTGCAGCCTCCGCGCAAGGAAGAAGCAGCGTTGATCGAC
ACGGCCATAAACGACAGCCTGGAAGTCTGGCCCCTCATCGCAGAAGGCAATTATCAGGCGGCAATGATGAGGCTGCATAC
CCAGAAGCAAAGCTGA

Upstream 100 bases:

>100_bases
TGGTTCGCAGGCATCCCAAGCCGTATCGCATGTTTTTTCAGCCCGTCGGAGTCATTCTGGCTTCGCGGGCTTTTCATTTT
GAGGATTCGGGTTACATTCA

Downstream 100 bases:

>100_bases
TATTTCCAAAGGACATGGGGTCATGAACTGTTTTGTTCCTCTTGATTCTACCTTCTCTCTCATTCACCCCTGAGCCTGTC
GAAGGGTCGAATGGCGTTCG

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 191; Mature: 191

Protein sequence:

>191_residues
MKLIVGLGNPGREYAATRHNAGAWWVVRLADQLGVTLKADGKFHGLCARIGQGESESWLLNPQTYMNASGRAVAALCRFY
RIQPEQMLVVHDELDLPPGVSRLKLDGGLGGHNGLKDIVAHLGTREFWRLRIGIGHPGEKHAVVNYVLQPPRKEEAALID
TAINDSLEVWPLIAEGNYQAAMMRLHTQKQS

Sequences:

>Translated_191_residues
MKLIVGLGNPGREYAATRHNAGAWWVVRLADQLGVTLKADGKFHGLCARIGQGESESWLLNPQTYMNASGRAVAALCRFY
RIQPEQMLVVHDELDLPPGVSRLKLDGGLGGHNGLKDIVAHLGTREFWRLRIGIGHPGEKHAVVNYVLQPPRKEEAALID
TAINDSLEVWPLIAEGNYQAAMMRLHTQKQS
>Mature_191_residues
MKLIVGLGNPGREYAATRHNAGAWWVVRLADQLGVTLKADGKFHGLCARIGQGESESWLLNPQTYMNASGRAVAALCRFY
RIQPEQMLVVHDELDLPPGVSRLKLDGGLGGHNGLKDIVAHLGTREFWRLRIGIGHPGEKHAVVNYVLQPPRKEEAALID
TAINDSLEVWPLIAEGNYQAAMMRLHTQKQS

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=148, Percent_Identity=38.5135135135135, Blast_Score=91, Evalue=6e-19,
Organism=Escherichia coli, GI1787455, Length=190, Percent_Identity=53.6842105263158, Blast_Score=182, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6321983, Length=143, Percent_Identity=32.1678321678322, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_NITMU (Q2YBH2)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411291.1
- ProteinModelPortal:   Q2YBH2
- SMR:   Q2YBH2
- STRING:   Q2YBH2
- GeneID:   3783990
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0591
- eggNOG:   COG0193
- HOGENOM:   HBG610927
- OMA:   AMHRLHS
- PhylomeDB:   Q2YBH2
- ProtClustDB:   PRK05426
- BioCyc:   NMUL323848:NMUL_A0591-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 21091; Mature: 21091

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLIVGLGNPGREYAATRHNAGAWWVVRLADQLGVTLKADGKFHGLCARIGQGESESWLL
CEEEEECCCCCHHHHHCCCCCCCEEEEEEHHHHCEEEEECCCEEEHHHHCCCCCCCCEEE
NPQTYMNASGRAVAALCRFYRIQPEQMLVVHDELDLPPGVSRLKLDGGLGGHNGLKDIVA
CCHHHCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCCCHHHHHH
HLGTREFWRLRIGIGHPGEKHAVVNYVLQPPRKEEAALIDTAINDSLEVWPLIAEGNYQA
HCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCCCCEEEEHHCCCCEEEEEEEECCCHHH
AMMRLHTQKQS
HHHHHHHCCCC
>Mature Secondary Structure
MKLIVGLGNPGREYAATRHNAGAWWVVRLADQLGVTLKADGKFHGLCARIGQGESESWLL
CEEEEECCCCCHHHHHCCCCCCCEEEEEEHHHHCEEEEECCCEEEHHHHCCCCCCCCEEE
NPQTYMNASGRAVAALCRFYRIQPEQMLVVHDELDLPPGVSRLKLDGGLGGHNGLKDIVA
CCHHHCCCCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEECCCCCCCCCHHHHHH
HLGTREFWRLRIGIGHPGEKHAVVNYVLQPPRKEEAALIDTAINDSLEVWPLIAEGNYQA
HCCCCEEEEEEEECCCCCCCCEEEEEEECCCCCCCCEEEEHHCCCCEEEEEEEECCCHHH
AMMRLHTQKQS
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA