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The map label for this gene is trmD

Identifier: 82701705

GI number: 82701705

Start: 657580

End: 658350

Strand: Direct

Name: trmD

Synonym: Nmul_A0571

Alternate gene names: 82701705

Gene position: 657580-658350 (Clockwise)

Preceding gene: 82701704

Following gene: 82701706

Centisome position: 20.65

GC content: 56.03

Gene sequence:

>771_bases
ATGACGTTTGATTTCGACGTTATCACCCTGTTCCCCGAGATGTTCAACGCTGTAACCAGGTACGGGGTAACCGGGCGGGC
GAATGAGAATGCCATCTATCGCCTGTATACCTGGAATCCGCGGGAATTTACCGAGGACAATTATCGCAGGGTGGACGACC
GGCCCTACGGGGGCGGACCCGGCATGGTAATGCTGGCAGAGCCCCTGGAAAAAGCGATAACTGCCGCCAGGGAAAGGCAA
AGAGCCTGTGGTATCGGCAGCACGAAAGTGGTTTATCTGTCACCTCAGGGAAGACCCCTGAACCAGAATGTGGTGATGGA
GTTAAGCGAATTGTCTGCATTGGTGCTGCTGGCAGGCCGTTATGAAGGAGTGGATGAGCGGCTGATAGAACGCCAGGTGG
ACTATGAGATTTCCATCGGGGATTATGTCGTATCCGGCGGTGAACTGGCGTCGATGGTGCTCATGGACTGTGTGGTGCGG
CAGCTGCCTGGCGTACTGGGGGATCCGGAATCCGCGAATCAGGATTCCTTTACAGCAGGTTTGCTGGATTTTCCCCACTA
CACCCGGCCGGAGGTCTATCGGGGAAGCGTAGTGCCCGAGGTCCTGTTGTCCGGAAATCACGCCAGGATTGAACGCTGGC
GGTTACAGCAGTCCCTTGGGAGAACATGGTTGCGGCGGCCCGACCTGCTGGCATTGAAAATGGAGAAAGGCCTCACCGCG
GAAGAGCGGAAATTGCTGGAAGAATTCCAGCATGCATACGAAGCGAATTGA

Upstream 100 bases:

>100_bases
AAGAAACCGAAAGACTGATACCTTTCATAGACCAGGTGATCATCAAAGTCGATCTGGCGGCGGGTCGGATTACGGTGGAT
TGGGGTCTGGACTACTGATT

Downstream 100 bases:

>100_bases
GCGCCAACTTGAAAGTTACTGAAACGCAAGGAATATAAGGCAAGGAGTCACGAAATGAATCTGATCGAGCAACTTGAAAG
CGAAGAAATCAGCCGCCTGG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 256; Mature: 255

Protein sequence:

>256_residues
MTFDFDVITLFPEMFNAVTRYGVTGRANENAIYRLYTWNPREFTEDNYRRVDDRPYGGGPGMVMLAEPLEKAITAARERQ
RACGIGSTKVVYLSPQGRPLNQNVVMELSELSALVLLAGRYEGVDERLIERQVDYEISIGDYVVSGGELASMVLMDCVVR
QLPGVLGDPESANQDSFTAGLLDFPHYTRPEVYRGSVVPEVLLSGNHARIERWRLQQSLGRTWLRRPDLLALKMEKGLTA
EERKLLEEFQHAYEAN

Sequences:

>Translated_256_residues
MTFDFDVITLFPEMFNAVTRYGVTGRANENAIYRLYTWNPREFTEDNYRRVDDRPYGGGPGMVMLAEPLEKAITAARERQ
RACGIGSTKVVYLSPQGRPLNQNVVMELSELSALVLLAGRYEGVDERLIERQVDYEISIGDYVVSGGELASMVLMDCVVR
QLPGVLGDPESANQDSFTAGLLDFPHYTRPEVYRGSVVPEVLLSGNHARIERWRLQQSLGRTWLRRPDLLALKMEKGLTA
EERKLLEEFQHAYEAN
>Mature_255_residues
TFDFDVITLFPEMFNAVTRYGVTGRANENAIYRLYTWNPREFTEDNYRRVDDRPYGGGPGMVMLAEPLEKAITAARERQR
ACGIGSTKVVYLSPQGRPLNQNVVMELSELSALVLLAGRYEGVDERLIERQVDYEISIGDYVVSGGELASMVLMDCVVRQ
LPGVLGDPESANQDSFTAGLLDFPHYTRPEVYRGSVVPEVLLSGNHARIERWRLQQSLGRTWLRRPDLLALKMEKGLTAE
ERKLLEEFQHAYEAN

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=252, Percent_Identity=57.9365079365079, Blast_Score=280, Evalue=7e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_NITMU (Q2YBJ2)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411271.1
- ProteinModelPortal:   Q2YBJ2
- SMR:   Q2YBJ2
- STRING:   Q2YBJ2
- GeneID:   3784791
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0571
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- PhylomeDB:   Q2YBJ2
- ProtClustDB:   PRK00026
- BioCyc:   NMUL323848:NMUL_A0571-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 29024; Mature: 28893

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: BINDING 119-119

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFDFDVITLFPEMFNAVTRYGVTGRANENAIYRLYTWNPREFTEDNYRRVDDRPYGGGP
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHCCCCCHHCCCCCCCCCCC
GMVMLAEPLEKAITAARERQRACGIGSTKVVYLSPQGRPLNQNVVMELSELSALVLLAGR
CEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
YEGVDERLIERQVDYEISIGDYVVSGGELASMVLMDCVVRQLPGVLGDPESANQDSFTAG
CCCHHHHHHHHHCCEEEECCCEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEH
LLDFPHYTRPEVYRGSVVPEVLLSGNHARIERWRLQQSLGRTWLRRPDLLALKMEKGLTA
HHCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCH
EERKLLEEFQHAYEAN
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TFDFDVITLFPEMFNAVTRYGVTGRANENAIYRLYTWNPREFTEDNYRRVDDRPYGGGP
CCCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCHHCCCCCHHCCCCCCCCCCC
GMVMLAEPLEKAITAARERQRACGIGSTKVVYLSPQGRPLNQNVVMELSELSALVLLAGR
CEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
YEGVDERLIERQVDYEISIGDYVVSGGELASMVLMDCVVRQLPGVLGDPESANQDSFTAG
CCCHHHHHHHHHCCEEEECCCEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEH
LLDFPHYTRPEVYRGSVVPEVLLSGNHARIERWRLQQSLGRTWLRRPDLLALKMEKGLTA
HHCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCH
EERKLLEEFQHAYEAN
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA