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The map label for this gene is pls [H]

Identifier: 82701668

GI number: 82701668

Start: 613763

End: 615859

Strand: Direct

Name: pls [H]

Synonym: Nmul_A0534

Alternate gene names: 82701668

Gene position: 613763-615859 (Clockwise)

Preceding gene: 82701665

Following gene: 82701669

Centisome position: 19.28

GC content: 56.7

Gene sequence:

>2097_bases
ATGTCCCCTTCCCACCAGCCCCCGGAAAGTCAGCCCACTGTCAAAGTTTTTGACCCGCTGGAGCTTGACCGTTCCGTGAT
TGCCATTCCTCTTCTGCGGCAGATGGAGGAGGAATTAAGAGGAATTGAAGCTTTTCGGGCTGCACATCCGTTCCCCGAAG
ATGGAGAATTCAATACGCTGATCGAATACAACAGGGAATTCGATGGAAAGCCGGAGGAGATGCGGGAGTTGGTCGTCAAG
ATGGCCGAAGAGGCGGCAGCGAAGGCATTGGAAGCATCGAAAAAACGAGTCGAAGAGAGCAAGGAGATAGCCCCAGCGGG
AACACGGGGCACGCCGGTGCGCGGTGGCTCGACAAGGAGTCGGGCTGATATTTACGGAAATCCTTTTTTGATGGAGCAAC
GGCGCGACAAGGATCTCCAGGAAGCGGTCGGGAATCAGAAAATCGGACCGGTCTCGGAGGACGGCGCCTATAGCTTTGCC
AGCTTGCACGCAACCATAATACGGCGAATATTAGCCGCAAACGAACGGCTGTCACAGGGTGAGGAAGCTTCAAAGAAACC
ACCTATCCTTCGGATCCACCCTACACGTTATGAGGTCATCATCGATATCAATCTCGAATATCCGGGTGGACGGGAAGAGG
CCCGGCGCTGGATTTACCAGAATATCGAAGAGGCCAAGAACAAAGCGAAAGTGTACGACGCGGGTCAGGATATTCATCTC
AAGAAGGAACAGCGCGAAAGCGGCTACATGTTCGCCTGCCTGGAAGCTCGTACCATCAAGGCGCTTATCGAACTCGATGT
TGCCCAGGCAAAGGTGAAGGCGCGCGAAGCTCAGGCAAAAGTGGGGGATACGAGCCATGAAGCGAAGGCTCGTGCAGCAA
AAATCAACCCGGCCAAGTTTCGCGCCATCTTCCGCATATGGCCGGATTTCGAGATTTCTGCCTCCATCACCCACTCCATC
GCCACGATAAAAGCCGATGCAGCGCAGAATTCTTTTTCCGCGCGGGGCGCCGGAATCACCTGGGCGGTGATGGATTCAGG
AATAAAGCAGGATCATGGGCACTTTCGCAAACACAACAATGTCGATAAAGACTCCACATGGCATAAAGACTTCACGGCGG
ACGGCAGCGATCCGTTCAACGATGAGAACGGGCATGGAACGCATGTGGCAGGCATCATTGCCGGCGAATGGCGTCCGGCA
GGTGCACCAGGAGGGACACAAGTGGCCGCGCCAGCGGAGGTTTCACCTCCGGCGGGCGATTCCGCCGCACCATCCCGAAT
TCCTGTAGCGGTTTCCCGTTATCTGAAAACGGGTTCCGAAGATGTCGAATACCAGCAGATAAAACTCGTGGACGGCATCA
GCGGGATGGCGCCGCGCTGCAAGCTGATAAGCCTGCGGGTGTTGGACGAAAACGGAAAAGGGAGTGTGAGCAATCTCATT
GCGGCCATCGCCCATGTGCAGGAGATCAACGGTTACGGCCGAAAGCTTCTGATCCACGGAGTCAACATGAGCCTGGGATA
TACCTTCGAGCCCGAATGGTTCGCCTGCGGCCAGAGTCCGCTCTGCGTGGAGGTTGACCGGCTGGTAAAAACCGGCGTCG
TGGTCGTGGTCGCCGCAGGCAATACAGGTTACGGCACGTTGAAGGCCGCCACAGGCGCGCTCTCGGCAGGAATGGCGCTG
ACCATCAATGATCCCGGCAATGCGGAACTCGCCATTACAGTGGGTTCGACCCACCGCGACATGCCGCACGTTTACGGGGT
TTCCTATTTTTCCTCGAAAGGCCCGACAGGCGACGGCCGCCTCAAGCCGGACCTCGTCGCGCCCGGCGAAAAAATCATCT
CCTGCGCCACGGGTCAGTTAAAAAGCGAGGGCGCACAGGGGATCGAATGCGACTACGTCGAAACCAGCGGCACGAGTATG
GCAGCTCCCCATGTCAGCGGCGCGATCGCAGCGTTTCTCTCGGTGCGCACCGAGTTCATCGGCAAGGCAGAACGTGTTAA
AGAGATTTTCGTTGATACGACTACTGACCTGCGGCGCGACCGCTACTTTCAGGGCAGTGGTCTCGTTGATCTGATGCGCG
CTATTCAGTCGGTGTGA

Upstream 100 bases:

>100_bases
AAGGGGACGTCAAAACCGGTGATCTGTTGTAAAAGCTGAAAAATAGCCTAATATCAGAATGCGAATGCAACGTTATCCCG
GCTCATGACAAGAGGTTCCG

Downstream 100 bases:

>100_bases
TTTTGAAAGTATAAAAAAGAGAAAAAAGGAGCACGGCCATGAATGAAATCGCGGGAATTCCTTATGTGGAAGCAAGCTTC
GACAAGAACGGTCAGGGTCC

Product: peptidase S8/S53 subtilisin kexin sedolisin

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 698; Mature: 697

Protein sequence:

>698_residues
MSPSHQPPESQPTVKVFDPLELDRSVIAIPLLRQMEEELRGIEAFRAAHPFPEDGEFNTLIEYNREFDGKPEEMRELVVK
MAEEAAAKALEASKKRVEESKEIAPAGTRGTPVRGGSTRSRADIYGNPFLMEQRRDKDLQEAVGNQKIGPVSEDGAYSFA
SLHATIIRRILAANERLSQGEEASKKPPILRIHPTRYEVIIDINLEYPGGREEARRWIYQNIEEAKNKAKVYDAGQDIHL
KKEQRESGYMFACLEARTIKALIELDVAQAKVKAREAQAKVGDTSHEAKARAAKINPAKFRAIFRIWPDFEISASITHSI
ATIKADAAQNSFSARGAGITWAVMDSGIKQDHGHFRKHNNVDKDSTWHKDFTADGSDPFNDENGHGTHVAGIIAGEWRPA
GAPGGTQVAAPAEVSPPAGDSAAPSRIPVAVSRYLKTGSEDVEYQQIKLVDGISGMAPRCKLISLRVLDENGKGSVSNLI
AAIAHVQEINGYGRKLLIHGVNMSLGYTFEPEWFACGQSPLCVEVDRLVKTGVVVVVAAGNTGYGTLKAATGALSAGMAL
TINDPGNAELAITVGSTHRDMPHVYGVSYFSSKGPTGDGRLKPDLVAPGEKIISCATGQLKSEGAQGIECDYVETSGTSM
AAPHVSGAIAAFLSVRTEFIGKAERVKEIFVDTTTDLRRDRYFQGSGLVDLMRAIQSV

Sequences:

>Translated_698_residues
MSPSHQPPESQPTVKVFDPLELDRSVIAIPLLRQMEEELRGIEAFRAAHPFPEDGEFNTLIEYNREFDGKPEEMRELVVK
MAEEAAAKALEASKKRVEESKEIAPAGTRGTPVRGGSTRSRADIYGNPFLMEQRRDKDLQEAVGNQKIGPVSEDGAYSFA
SLHATIIRRILAANERLSQGEEASKKPPILRIHPTRYEVIIDINLEYPGGREEARRWIYQNIEEAKNKAKVYDAGQDIHL
KKEQRESGYMFACLEARTIKALIELDVAQAKVKAREAQAKVGDTSHEAKARAAKINPAKFRAIFRIWPDFEISASITHSI
ATIKADAAQNSFSARGAGITWAVMDSGIKQDHGHFRKHNNVDKDSTWHKDFTADGSDPFNDENGHGTHVAGIIAGEWRPA
GAPGGTQVAAPAEVSPPAGDSAAPSRIPVAVSRYLKTGSEDVEYQQIKLVDGISGMAPRCKLISLRVLDENGKGSVSNLI
AAIAHVQEINGYGRKLLIHGVNMSLGYTFEPEWFACGQSPLCVEVDRLVKTGVVVVVAAGNTGYGTLKAATGALSAGMAL
TINDPGNAELAITVGSTHRDMPHVYGVSYFSSKGPTGDGRLKPDLVAPGEKIISCATGQLKSEGAQGIECDYVETSGTSM
AAPHVSGAIAAFLSVRTEFIGKAERVKEIFVDTTTDLRRDRYFQGSGLVDLMRAIQSV
>Mature_697_residues
SPSHQPPESQPTVKVFDPLELDRSVIAIPLLRQMEEELRGIEAFRAAHPFPEDGEFNTLIEYNREFDGKPEEMRELVVKM
AEEAAAKALEASKKRVEESKEIAPAGTRGTPVRGGSTRSRADIYGNPFLMEQRRDKDLQEAVGNQKIGPVSEDGAYSFAS
LHATIIRRILAANERLSQGEEASKKPPILRIHPTRYEVIIDINLEYPGGREEARRWIYQNIEEAKNKAKVYDAGQDIHLK
KEQRESGYMFACLEARTIKALIELDVAQAKVKAREAQAKVGDTSHEAKARAAKINPAKFRAIFRIWPDFEISASITHSIA
TIKADAAQNSFSARGAGITWAVMDSGIKQDHGHFRKHNNVDKDSTWHKDFTADGSDPFNDENGHGTHVAGIIAGEWRPAG
APGGTQVAAPAEVSPPAGDSAAPSRIPVAVSRYLKTGSEDVEYQQIKLVDGISGMAPRCKLISLRVLDENGKGSVSNLIA
AIAHVQEINGYGRKLLIHGVNMSLGYTFEPEWFACGQSPLCVEVDRLVKTGVVVVVAAGNTGYGTLKAATGALSAGMALT
INDPGNAELAITVGSTHRDMPHVYGVSYFSSKGPTGDGRLKPDLVAPGEKIISCATGQLKSEGAQGIECDYVETSGTSMA
APHVSGAIAAFLSVRTEFIGKAERVKEIFVDTTTDLRRDRYFQGSGLVDLMRAIQSV

Specific function: Has endopeptidase activity toward caseins, casein fragments including alpha-S1-casein and synthetic peptides [H]

COG id: COG1404

COG function: function code O; Subtilisin-like serine proteases

Gene ontology:

Cell location: Cell envelope. Note=Cell envelope associated [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S8 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007280
- InterPro:   IPR000209
- InterPro:   IPR022398
- InterPro:   IPR015500 [H]

Pfam domain/function: PF00082 Peptidase_S8; PF04151 PPC [H]

EC number: NA

Molecular weight: Translated: 75765; Mature: 75634

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00137 SUBTILASE_HIS ; PS00138 SUBTILASE_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPSHQPPESQPTVKVFDPLELDRSVIAIPLLRQMEEELRGIEAFRAAHPFPEDGEFNTL
CCCCCCCCCCCCCEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
IEYNREFDGKPEEMRELVVKMAEEAAAKALEASKKRVEESKEIAPAGTRGTPVRGGSTRS
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
RADIYGNPFLMEQRRDKDLQEAVGNQKIGPVSEDGAYSFASLHATIIRRILAANERLSQG
CCCCCCCCEEECHHCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
EEASKKPPILRIHPTRYEVIIDINLEYPGGREEARRWIYQNIEEAKNKAKVYDAGQDIHL
CCCCCCCCEEEECCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCEEEECCCCCEE
KKEQRESGYMFACLEARTIKALIELDVAQAKVKAREAQAKVGDTSHEAKARAAKINPAKF
CHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHH
RAIFRIWPDFEISASITHSIATIKADAAQNSFSARGAGITWAVMDSGIKQDHGHFRKHNN
EEEEEECCCCEECHHHHHHHHEEEEHHCCCCCCCCCCCEEEEEECCCCHHHCCHHHHCCC
VDKDSTWHKDFTADGSDPFNDENGHGTHVAGIIAGEWRPAGAPGGTQVAAPAEVSPPAGD
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEECCCCCCCCCCCC
SAAPSRIPVAVSRYLKTGSEDVEYQQIKLVDGISGMAPRCKLISLRVLDENGKGSVSNLI
CCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCEEEEEEEEECCCCCCHHHHHH
AAIAHVQEINGYGRKLLIHGVNMSLGYTFEPEWFACGQSPLCVEVDRLVKTGVVVVVAAG
HHHHHHHHHCCCCCEEEEEECCCEECEEECCCCEECCCCCCEEEHHHHHHCCEEEEEEEC
NTGYGTLKAATGALSAGMALTINDPGNAELAITVGSTHRDMPHVYGVSYFSSKGPTGDGR
CCCCCCHHHHHHHHHCCEEEEECCCCCCEEEEEECCCCCCCCHHHEEHHHCCCCCCCCCC
LKPDLVAPGEKIISCATGQLKSEGAQGIECDYVETSGTSMAAPHVSGAIAAFLSVRTEFI
CCCCCCCCCHHHHHHHCCCHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
GKAERVKEIFVDTTTDLRRDRYFQGSGLVDLMRAIQSV
CHHHHHHHHHHCCCHHHHHHCEECCCCHHHHHHHHHCC
>Mature Secondary Structure 
SPSHQPPESQPTVKVFDPLELDRSVIAIPLLRQMEEELRGIEAFRAAHPFPEDGEFNTL
CCCCCCCCCCCCEEEECCHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
IEYNREFDGKPEEMRELVVKMAEEAAAKALEASKKRVEESKEIAPAGTRGTPVRGGSTRS
EEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCC
RADIYGNPFLMEQRRDKDLQEAVGNQKIGPVSEDGAYSFASLHATIIRRILAANERLSQG
CCCCCCCCEEECHHCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCC
EEASKKPPILRIHPTRYEVIIDINLEYPGGREEARRWIYQNIEEAKNKAKVYDAGQDIHL
CCCCCCCCEEEECCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHCEEEECCCCCEE
KKEQRESGYMFACLEARTIKALIELDVAQAKVKAREAQAKVGDTSHEAKARAAKINPAKF
CHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHH
RAIFRIWPDFEISASITHSIATIKADAAQNSFSARGAGITWAVMDSGIKQDHGHFRKHNN
EEEEEECCCCEECHHHHHHHHEEEEHHCCCCCCCCCCCEEEEEECCCCHHHCCHHHHCCC
VDKDSTWHKDFTADGSDPFNDENGHGTHVAGIIAGEWRPAGAPGGTQVAAPAEVSPPAGD
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCEECCCCCCCCCCCC
SAAPSRIPVAVSRYLKTGSEDVEYQQIKLVDGISGMAPRCKLISLRVLDENGKGSVSNLI
CCCCCCCHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCEEEEEEEEECCCCCCHHHHHH
AAIAHVQEINGYGRKLLIHGVNMSLGYTFEPEWFACGQSPLCVEVDRLVKTGVVVVVAAG
HHHHHHHHHCCCCCEEEEEECCCEECEEECCCCEECCCCCCEEEHHHHHHCCEEEEEEEC
NTGYGTLKAATGALSAGMALTINDPGNAELAITVGSTHRDMPHVYGVSYFSSKGPTGDGR
CCCCCCHHHHHHHHHCCEEEEECCCCCCEEEEEECCCCCCCCHHHEEHHHCCCCCCCCCC
LKPDLVAPGEKIISCATGQLKSEGAQGIECDYVETSGTSMAAPHVSGAIAAFLSVRTEFI
CCCCCCCCCHHHHHHHCCCHHCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHH
GKAERVKEIFVDTTTDLRRDRYFQGSGLVDLMRAIQSV
CHHHHHHHHHHCCCHHHHHHCEECCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8702780; 11210516 [H]