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The map label for this gene is alkJ [H]

Identifier: 82701659

GI number: 82701659

Start: 597597

End: 599657

Strand: Reverse

Name: alkJ [H]

Synonym: Nmul_A0525

Alternate gene names: 82701659

Gene position: 599657-597597 (Counterclockwise)

Preceding gene: 82701660

Following gene: 82701658

Centisome position: 18.83

GC content: 56.77

Gene sequence:

>2061_bases
TTGCAAAACGGAGGCCAAACCGAAGGGAAGTTGCAGCCAAACCGGCGGAAACTTTTCCTTTATAATCATTTTCGCGACTC
TCCGCAGAAAAATACGATTGATTTCGCCTTGATCTCCCGACGCCGTTTTGTCAAGCTCACCGCCCTGCTGGTATCCGGCG
CAAGCACCTTTCTTGCCAGTTGCTCTACCCTTCTCTGGAAGGGGTCCTCGCGCCCCTTTGTCCACCTGAATTGTCCCTCC
GCTGCCGGGAACAAATTCGATTACATCATCATCGGTTCCGGAGCGGGCGGAGGTCCGCTCGCCTGCAACCTCGCTTTGAA
GGGTTTCCGCGTGCTGCTGCTGGAGGCTGGGGGAGAAGACGATCCCTGCGAATATTACGTTCCCGCCTTTCACGCGCGCG
CCTCCGAGCACGAGGCGCTGCGCTGGGACTTCTTTGTGCGTCACTATGCGGACGACGAGCAGCAGAAACGGGATGACAAG
TTCATTCCGGAACGGAACGGCGTGCTGTACCCGCGCTCGGGGACCTTGGGGGGATGTACCGCGCACAATGCCATGCTGTT
GATCTATCCCCACAATAGCGATTGGGACTATATCGCCGAAAAGACCGGCGACGAATCCTGGCGCAGCGAAAATATGCAGA
AATACTTCAAGCGCATGGAGCGCTGCGAGTATGCCACCCGGGCTGAAGAACCGGATAGCCGCCATGGATTCGGAGGCTGG
CTTCCAACAAATCTGGCAGAGCGGGAAATGCTGCTGAGGGACAAAGTCCTTAGGAGGCTCTCAAAAGCCGCGCTCGAGGA
ATCACTCAGCGGCATTATCAACCCGATCAAACGGACCTTGCTCAAGATAAAAACCCATGGTGATCCCAACGATTGGCGTT
TTATCCGGAGAAGGCCAGAAGGCTTGTGCAAGATACCGATCACCACCCGGCAGGGGCGTCGGGCTGGAGCCCGGGAGTAC
ATATTGCGCGTTCGCGAGCAGTGTCCGAATTTTCTCGAAGTCAGAACTCATGCGCTGGCGAAGCGTATCCTGCTCGATGA
ACAGAACAAGGCCTATGGAGTGGAGTACATTTCCGGCGCCCATCAGTACCGTGCGGATCCGCGACATGGGACAGCAGCCC
CGGGGATCGGGGAAACCGTGCTTGCCGAACGGGAAGTGATCGTTTCCGCAGGCGCATTCAATACACCCCAGTTGCTCAAG
CTCTCCGGCATCGGTCCTCGGGAAGAACTGGAGGGGCATGGCATCACGGTAAAAGTAGACTTGCCAGGGGTGGGAGAAAA
CCTCCAAGATCGTTATGAACTGGGCGTGGTTACCCGGCTCAGGGAAAACATCCCGCTGATCAAGGGAATGAAGCTCCGCT
GTCCCGCCCCGGGCGAGGCGCCTGATTCCCAGTTCAGCCAGTGGCTTCAGGGCGTCGGGCCCTACACCACCAATGGTGCG
ACGATCGCGCTCATGAAGCGCTCGTTTCCAGAACGCCCGGATCCCGATTTATTCATTTTCGGGCTGATCGGGGATTTCCG
CGGATATTATCCGGGTTATGCCAATAACATTGCCAGGACAGAAAACGCCTTTACCTGGGCCATCCTGAAGGGCCATACCG
CCAATACAGCCGGGCGGGTCACGCTGCGGTCCGCCGATCCGCGCGATGTGCCGGATATAAACTTTCATTATTTTTCGGAA
GGCACGAACGCTGGAGAAGACATGGAGTCACTTGTCGCCGGAGTGGAAGCAGTGCGCAATATCGTCAGGCTCTCCTCCGA
CGTGATCGAGGAGGAGGTTCTGCCTGGCCCGGAAGTCAAGAGCCGTGAAGAGATACAACGTTTTATAAAAAACCAGGCGT
GGGGACATCATGCCTCCTGCACTTGTAAAATGGGCGCCGCCACTGATCCCATGGCTGTCGTCGACAGCCGGTTTCGCGTT
TATGGCACCCGGAACCTGCGCATTGTGGATGCTTCCGTTTTCCCCAAAATACCCGGGTTCTTCATCGCTTCATCCATCTA
CATGATAAGCGAAAAAGCGTGCGACGTGATCCGCGAGGATGCGGAAAGGATGCAGGTCTGA

Upstream 100 bases:

>100_bases
AAACGCGCATTGATCGAATTCCTGAAAACGATGTGACGCGCCGCGTGAGGGATCACCACCAGAAGCACCGCTCCACCGAG
GGGAGCGGTGCGAGTTCTTA

Downstream 100 bases:

>100_bases
TAGGGTTTCCATATCTTCCGCCTCCTCCTGGTCGTGCCCTCTCCCGGAATTTGACACCGGCGGCAGCGGAAGGTGAGCCG
CACTTATATTCCCGTTCATG

Product: glucose-methanol-choline oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 686; Mature: 686

Protein sequence:

>686_residues
MQNGGQTEGKLQPNRRKLFLYNHFRDSPQKNTIDFALISRRRFVKLTALLVSGASTFLASCSTLLWKGSSRPFVHLNCPS
AAGNKFDYIIIGSGAGGGPLACNLALKGFRVLLLEAGGEDDPCEYYVPAFHARASEHEALRWDFFVRHYADDEQQKRDDK
FIPERNGVLYPRSGTLGGCTAHNAMLLIYPHNSDWDYIAEKTGDESWRSENMQKYFKRMERCEYATRAEEPDSRHGFGGW
LPTNLAEREMLLRDKVLRRLSKAALEESLSGIINPIKRTLLKIKTHGDPNDWRFIRRRPEGLCKIPITTRQGRRAGAREY
ILRVREQCPNFLEVRTHALAKRILLDEQNKAYGVEYISGAHQYRADPRHGTAAPGIGETVLAEREVIVSAGAFNTPQLLK
LSGIGPREELEGHGITVKVDLPGVGENLQDRYELGVVTRLRENIPLIKGMKLRCPAPGEAPDSQFSQWLQGVGPYTTNGA
TIALMKRSFPERPDPDLFIFGLIGDFRGYYPGYANNIARTENAFTWAILKGHTANTAGRVTLRSADPRDVPDINFHYFSE
GTNAGEDMESLVAGVEAVRNIVRLSSDVIEEEVLPGPEVKSREEIQRFIKNQAWGHHASCTCKMGAATDPMAVVDSRFRV
YGTRNLRIVDASVFPKIPGFFIASSIYMISEKACDVIREDAERMQV

Sequences:

>Translated_686_residues
MQNGGQTEGKLQPNRRKLFLYNHFRDSPQKNTIDFALISRRRFVKLTALLVSGASTFLASCSTLLWKGSSRPFVHLNCPS
AAGNKFDYIIIGSGAGGGPLACNLALKGFRVLLLEAGGEDDPCEYYVPAFHARASEHEALRWDFFVRHYADDEQQKRDDK
FIPERNGVLYPRSGTLGGCTAHNAMLLIYPHNSDWDYIAEKTGDESWRSENMQKYFKRMERCEYATRAEEPDSRHGFGGW
LPTNLAEREMLLRDKVLRRLSKAALEESLSGIINPIKRTLLKIKTHGDPNDWRFIRRRPEGLCKIPITTRQGRRAGAREY
ILRVREQCPNFLEVRTHALAKRILLDEQNKAYGVEYISGAHQYRADPRHGTAAPGIGETVLAEREVIVSAGAFNTPQLLK
LSGIGPREELEGHGITVKVDLPGVGENLQDRYELGVVTRLRENIPLIKGMKLRCPAPGEAPDSQFSQWLQGVGPYTTNGA
TIALMKRSFPERPDPDLFIFGLIGDFRGYYPGYANNIARTENAFTWAILKGHTANTAGRVTLRSADPRDVPDINFHYFSE
GTNAGEDMESLVAGVEAVRNIVRLSSDVIEEEVLPGPEVKSREEIQRFIKNQAWGHHASCTCKMGAATDPMAVVDSRFRV
YGTRNLRIVDASVFPKIPGFFIASSIYMISEKACDVIREDAERMQV
>Mature_686_residues
MQNGGQTEGKLQPNRRKLFLYNHFRDSPQKNTIDFALISRRRFVKLTALLVSGASTFLASCSTLLWKGSSRPFVHLNCPS
AAGNKFDYIIIGSGAGGGPLACNLALKGFRVLLLEAGGEDDPCEYYVPAFHARASEHEALRWDFFVRHYADDEQQKRDDK
FIPERNGVLYPRSGTLGGCTAHNAMLLIYPHNSDWDYIAEKTGDESWRSENMQKYFKRMERCEYATRAEEPDSRHGFGGW
LPTNLAEREMLLRDKVLRRLSKAALEESLSGIINPIKRTLLKIKTHGDPNDWRFIRRRPEGLCKIPITTRQGRRAGAREY
ILRVREQCPNFLEVRTHALAKRILLDEQNKAYGVEYISGAHQYRADPRHGTAAPGIGETVLAEREVIVSAGAFNTPQLLK
LSGIGPREELEGHGITVKVDLPGVGENLQDRYELGVVTRLRENIPLIKGMKLRCPAPGEAPDSQFSQWLQGVGPYTTNGA
TIALMKRSFPERPDPDLFIFGLIGDFRGYYPGYANNIARTENAFTWAILKGHTANTAGRVTLRSADPRDVPDINFHYFSE
GTNAGEDMESLVAGVEAVRNIVRLSSDVIEEEVLPGPEVKSREEIQRFIKNQAWGHHASCTCKMGAATDPMAVVDSRFRV
YGTRNLRIVDASVFPKIPGFFIASSIYMISEKACDVIREDAERMQV

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=621, Percent_Identity=28.341384863124, Blast_Score=184, Evalue=3e-46,
Organism=Escherichia coli, GI1786503, Length=612, Percent_Identity=28.4313725490196, Blast_Score=164, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17532301, Length=625, Percent_Identity=28.48, Blast_Score=193, Evalue=3e-49,
Organism=Drosophila melanogaster, GI45551458, Length=676, Percent_Identity=29.1420118343195, Blast_Score=187, Evalue=1e-47,
Organism=Drosophila melanogaster, GI45549471, Length=640, Percent_Identity=29.53125, Blast_Score=186, Evalue=4e-47,
Organism=Drosophila melanogaster, GI18859993, Length=624, Percent_Identity=28.525641025641, Blast_Score=185, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24642055, Length=634, Percent_Identity=28.391167192429, Blast_Score=179, Evalue=6e-45,
Organism=Drosophila melanogaster, GI24642042, Length=649, Percent_Identity=28.3513097072419, Blast_Score=174, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24642037, Length=622, Percent_Identity=27.9742765273312, Blast_Score=168, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24650267, Length=634, Percent_Identity=27.1293375394322, Blast_Score=163, Evalue=4e-40,
Organism=Drosophila melanogaster, GI24642039, Length=632, Percent_Identity=27.5316455696203, Blast_Score=162, Evalue=7e-40,
Organism=Drosophila melanogaster, GI24642051, Length=630, Percent_Identity=26.3492063492064, Blast_Score=160, Evalue=3e-39,
Organism=Drosophila melanogaster, GI24642035, Length=661, Percent_Identity=26.6263237518911, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24642059, Length=630, Percent_Identity=27.3015873015873, Blast_Score=155, Evalue=1e-37,
Organism=Drosophila melanogaster, GI18859995, Length=633, Percent_Identity=26.6982622432859, Blast_Score=149, Evalue=8e-36,
Organism=Drosophila melanogaster, GI17137792, Length=621, Percent_Identity=26.0869565217391, Blast_Score=145, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24642048, Length=633, Percent_Identity=27.172195892575, Blast_Score=139, Evalue=7e-33,
Organism=Drosophila melanogaster, GI24642057, Length=416, Percent_Identity=27.6442307692308, Blast_Score=99, Evalue=8e-21,
Organism=Drosophila melanogaster, GI24645930, Length=583, Percent_Identity=24.0137221269297, Blast_Score=83, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 76979; Mature: 76979

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNGGQTEGKLQPNRRKLFLYNHFRDSPQKNTIDFALISRRRFVKLTALLVSGASTFLAS
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEHEEHHHHHHHHHHHHHHHCHHHHHHH
CSTLLWKGSSRPFVHLNCPSAAGNKFDYIIIGSGAGGGPLACNLALKGFRVLLLEAGGED
HHHHHCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCEEEEEECCCEEEEEEECCCCC
DPCEYYVPAFHARASEHEALRWDFFVRHYADDEQQKRDDKFIPERNGVLYPRSGTLGGCT
CCCCEECCHHHCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEECCCCCCCCEE
AHNAMLLIYPHNSDWDYIAEKTGDESWRSENMQKYFKRMERCEYATRAEEPDSRHGFGGW
CCCEEEEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
LPTNLAEREMLLRDKVLRRLSKAALEESLSGIINPIKRTLLKIKTHGDPNDWRFIRRRPE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHCCCC
GLCKIPITTRQGRRAGAREYILRVREQCPNFLEVRTHALAKRILLDEQNKAYGVEYISGA
CEEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEECHHHHCCC
HQYRADPRHGTAAPGIGETVLAEREVIVSAGAFNTPQLLKLSGIGPREELEGHGITVKVD
HHHCCCCCCCCCCCCCCHHHHHCCCEEEECCCCCCCCEEEECCCCCHHHHCCCCEEEEEE
LPGVGENLQDRYELGVVTRLRENIPLIKGMKLRCPAPGEAPDSQFSQWLQGVGPYTTNGA
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCC
TIALMKRSFPERPDPDLFIFGLIGDFRGYYPGYANNIARTENAFTWAILKGHTANTAGRV
EEEEEECCCCCCCCCCEEEEEEHHHHCCCCCCHHCCCCCCCCCEEEEEEECCCCCCCCEE
TLRSADPRDVPDINFHYFSEGTNAGEDMESLVAGVEAVRNIVRLSSDVIEEEVLPGPEVK
EEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SREEIQRFIKNQAWGHHASCTCKMGAATDPMAVVDSRFRVYGTRNLRIVDASVFPKIPGF
CHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHCCCEEEEECCCEEEEEHHHCCCCCCH
FIASSIYMISEKACDVIREDAERMQV
HHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQNGGQTEGKLQPNRRKLFLYNHFRDSPQKNTIDFALISRRRFVKLTALLVSGASTFLAS
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEHEEHHHHHHHHHHHHHHHCHHHHHHH
CSTLLWKGSSRPFVHLNCPSAAGNKFDYIIIGSGAGGGPLACNLALKGFRVLLLEAGGED
HHHHHCCCCCCCEEEEECCCCCCCCEEEEEEECCCCCCCEEEEEECCCEEEEEEECCCCC
DPCEYYVPAFHARASEHEALRWDFFVRHYADDEQQKRDDKFIPERNGVLYPRSGTLGGCT
CCCCEECCHHHCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCEEECCCCCCCCEE
AHNAMLLIYPHNSDWDYIAEKTGDESWRSENMQKYFKRMERCEYATRAEEPDSRHGFGGW
CCCEEEEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
LPTNLAEREMLLRDKVLRRLSKAALEESLSGIINPIKRTLLKIKTHGDPNDWRFIRRRPE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHCCCC
GLCKIPITTRQGRRAGAREYILRVREQCPNFLEVRTHALAKRILLDEQNKAYGVEYISGA
CEEEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEECHHHHCCC
HQYRADPRHGTAAPGIGETVLAEREVIVSAGAFNTPQLLKLSGIGPREELEGHGITVKVD
HHHCCCCCCCCCCCCCCHHHHHCCCEEEECCCCCCCCEEEECCCCCHHHHCCCCEEEEEE
LPGVGENLQDRYELGVVTRLRENIPLIKGMKLRCPAPGEAPDSQFSQWLQGVGPYTTNGA
CCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHHHHHHHCCCCCCCCCC
TIALMKRSFPERPDPDLFIFGLIGDFRGYYPGYANNIARTENAFTWAILKGHTANTAGRV
EEEEEECCCCCCCCCCEEEEEEHHHHCCCCCCHHCCCCCCCCCEEEEEEECCCCCCCCEE
TLRSADPRDVPDINFHYFSEGTNAGEDMESLVAGVEAVRNIVRLSSDVIEEEVLPGPEVK
EEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
SREEIQRFIKNQAWGHHASCTCKMGAATDPMAVVDSRFRVYGTRNLRIVDASVFPKIPGF
CHHHHHHHHHHCCCCCCCCEEEECCCCCCCHHHHCCCEEEEECCCEEEEEHHHCCCCCCH
FIASSIYMISEKACDVIREDAERMQV
HHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11390693 [H]