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The map label for this gene is mpl [H]

Identifier: 82701657

GI number: 82701657

Start: 594921

End: 596291

Strand: Direct

Name: mpl [H]

Synonym: Nmul_A0523

Alternate gene names: 82701657

Gene position: 594921-596291 (Clockwise)

Preceding gene: 82701656

Following gene: 82701662

Centisome position: 18.68

GC content: 53.98

Gene sequence:

>1371_bases
TTGCATATTCATATCCTTGGCATCTGCGGCACATTCATGGGAGGCATTGCGGCAATCGCACAGGAAGCGGGGCACAAGGT
TACCGGTTGCGATGCAGGCGTCTATCCCCCGATGAGCACGCAATTGAAATCACGGGGTATCGAGTTGATCGAAGGATTTT
CCTCGGAACAAACCAGGCTGAATCCTGACCTGTTCGTTATAGGAAACGTGGTGAGCCGCGGCAATCCCCTCATGGAAGAG
ATTCTTGACCGCAACCTGCCCTATATTTCCGGTCCTCAATGGCTTTCGGAGAATGTCCTGCGGGACAAATGGGTGCTTGC
GGTCGCGGGAACGCATGGTAAGACCACAACCAGTTCCATGCTGGCGTGGATTCTCGAATATGCCGGAATGGAACCGGGAT
TTCTCATTGGCGGCATACCGCAGAACTTTGGAATCTCGGCCAGATTGGGGAATTCCGGTTTTTTCGTCATTGAAGCCGAT
GAATACGATACGGCTTTTTTTGACAAGCGTTCCAAGTTTGTCCATTTTCATCCAAGAACAGCCATTCTCAATAACCTGGA
ATACGATCATGCAGATATTTTCAGGAACCTTGAGGAGATCGAGCAGCAGTTTCACCACCTCGTGCGTGTCGTTCCCGGCA
AGGGTCTTATCGTCAGCAATGGCCAGGAAGAGAATCTGGAGCGGGTCTTGCGGAAGGGATGCTGGACACCCGTCGAAAGA
ATAGGCGTCGCCAATGGGTGGGAGGCAGACGTCCTCACGGATGGTGCAGTTGCCGTATCCTTTCTGGGGGAATCGCAGGG
AACGCTGCTATGGGAATTGTTGGGAAAGCACAACCGGATGAATGCACTCGCCGCACTGGCTGCTGCTCGTCACGCCGGTG
TAGCGGTAAAGGCAGGCATTGCCGCACTGGCGGAATTCAGGAATGTCAAGCGCCGCATGGAAGTAAAGGGTATAACAAGG
GACATCACGGTCTATGACGATTTTGCCCATCACCCGACAGCGATTCGGACAACGATTGACGGGCTGCGCAGCAGGGTGAA
GGGGGCGCGCATCATCGCCGTGCTTGAGCCACGTTCGAACACCATGAAAATGGGCGTATGGAAAGAAAGTCTGGCAGACA
GCCTAACAGGGGCGGACGTAGTGTTCTGCTACGCCGCCAACCTGGGCTGGGATTTGCCGCAAGCGCTGCTTCCACTGGGC
GAAAAGGCGGTTGCCTTTGAAGAGCTGGATCAATTGATAGCCGCGATTACGGCATATGCCCAACCAGGCGACCATGTACT
CATCATGAGCAATGCCGGTTTTGGCGGTATTCACGAAAAACTGCTCACAAGGATGGCTGCAGTTGCAGCAGACGAAGTTG
CCAGCGGGTAA

Upstream 100 bases:

>100_bases
AAGCACGCTGACAGGATGGAACGGCTCAATTCCGGGCGGGCATATTTTAATCGCAAGCAGGCGGACGCGTAATTCATTTT
ATATACATCAGGAGCAAGCT

Downstream 100 bases:

>100_bases
AGGTCAAGCCGCCGTCAATCAGACAGACAGATATGGCAGGACAGGAACCGGATTGACAATCACCGCGGTGATTTTATCTT
GCCGCAATTGGCGCTGATCC

Product: UDP-N-acetylmuramate

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MHIHILGICGTFMGGIAAIAQEAGHKVTGCDAGVYPPMSTQLKSRGIELIEGFSSEQTRLNPDLFVIGNVVSRGNPLMEE
ILDRNLPYISGPQWLSENVLRDKWVLAVAGTHGKTTTSSMLAWILEYAGMEPGFLIGGIPQNFGISARLGNSGFFVIEAD
EYDTAFFDKRSKFVHFHPRTAILNNLEYDHADIFRNLEEIEQQFHHLVRVVPGKGLIVSNGQEENLERVLRKGCWTPVER
IGVANGWEADVLTDGAVAVSFLGESQGTLLWELLGKHNRMNALAALAAARHAGVAVKAGIAALAEFRNVKRRMEVKGITR
DITVYDDFAHHPTAIRTTIDGLRSRVKGARIIAVLEPRSNTMKMGVWKESLADSLTGADVVFCYAANLGWDLPQALLPLG
EKAVAFEELDQLIAAITAYAQPGDHVLIMSNAGFGGIHEKLLTRMAAVAADEVASG

Sequences:

>Translated_456_residues
MHIHILGICGTFMGGIAAIAQEAGHKVTGCDAGVYPPMSTQLKSRGIELIEGFSSEQTRLNPDLFVIGNVVSRGNPLMEE
ILDRNLPYISGPQWLSENVLRDKWVLAVAGTHGKTTTSSMLAWILEYAGMEPGFLIGGIPQNFGISARLGNSGFFVIEAD
EYDTAFFDKRSKFVHFHPRTAILNNLEYDHADIFRNLEEIEQQFHHLVRVVPGKGLIVSNGQEENLERVLRKGCWTPVER
IGVANGWEADVLTDGAVAVSFLGESQGTLLWELLGKHNRMNALAALAAARHAGVAVKAGIAALAEFRNVKRRMEVKGITR
DITVYDDFAHHPTAIRTTIDGLRSRVKGARIIAVLEPRSNTMKMGVWKESLADSLTGADVVFCYAANLGWDLPQALLPLG
EKAVAFEELDQLIAAITAYAQPGDHVLIMSNAGFGGIHEKLLTRMAAVAADEVASG
>Mature_456_residues
MHIHILGICGTFMGGIAAIAQEAGHKVTGCDAGVYPPMSTQLKSRGIELIEGFSSEQTRLNPDLFVIGNVVSRGNPLMEE
ILDRNLPYISGPQWLSENVLRDKWVLAVAGTHGKTTTSSMLAWILEYAGMEPGFLIGGIPQNFGISARLGNSGFFVIEAD
EYDTAFFDKRSKFVHFHPRTAILNNLEYDHADIFRNLEEIEQQFHHLVRVVPGKGLIVSNGQEENLERVLRKGCWTPVER
IGVANGWEADVLTDGAVAVSFLGESQGTLLWELLGKHNRMNALAALAAARHAGVAVKAGIAALAEFRNVKRRMEVKGITR
DITVYDDFAHHPTAIRTTIDGLRSRVKGARIIAVLEPRSNTMKMGVWKESLADSLTGADVVFCYAANLGWDLPQALLPLG
EKAVAFEELDQLIAAITAYAQPGDHVLIMSNAGFGGIHEKLLTRMAAVAADEVASG

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=453, Percent_Identity=56.7328918322296, Blast_Score=518, Evalue=1e-148,
Organism=Escherichia coli, GI1786279, Length=401, Percent_Identity=28.6783042394015, Blast_Score=115, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 49633; Mature: 49633

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIHILGICGTFMGGIAAIAQEAGHKVTGCDAGVYPPMSTQLKSRGIELIEGFSSEQTRL
CEEEEEEHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHCCHHHHHCCCCCCCCC
NPDLFVIGNVVSRGNPLMEEILDRNLPYISGPQWLSENVLRDKWVLAVAGTHGKTTTSSM
CCCEEEEECHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHH
LAWILEYAGMEPGFLIGGIPQNFGISARLGNSGFFVIEADEYDTAFFDKRSKFVHFHPRT
HHHHHHHHCCCCCEEECCCCCCCCCEEEECCCCEEEEECCCCCCHHHHCCCCEEEECCHH
AILNNLEYDHADIFRNLEEIEQQFHHLVRVVPGKGLIVSNGQEENLERVLRKGCWTPVER
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHCCCCCHHH
IGVANGWEADVLTDGAVAVSFLGESQGTLLWELLGKHNRMNALAALAAARHAGVAVKAGI
CCCCCCCCCCEECCCCEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHH
AALAEFRNVKRRMEVKGITRDITVYDDFAHHPTAIRTTIDGLRSRVKGARIIAVLEPRSN
HHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCC
TMKMGVWKESLADSLTGADVVFCYAANLGWDLPQALLPLGEKAVAFEELDQLIAAITAYA
CEEECCHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHC
QPGDHVLIMSNAGFGGIHEKLLTRMAAVAADEVASG
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MHIHILGICGTFMGGIAAIAQEAGHKVTGCDAGVYPPMSTQLKSRGIELIEGFSSEQTRL
CEEEEEEHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCHHHHHHCCHHHHHCCCCCCCCC
NPDLFVIGNVVSRGNPLMEEILDRNLPYISGPQWLSENVLRDKWVLAVAGTHGKTTTSSM
CCCEEEEECHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEEECCCCCCHHHHH
LAWILEYAGMEPGFLIGGIPQNFGISARLGNSGFFVIEADEYDTAFFDKRSKFVHFHPRT
HHHHHHHHCCCCCEEECCCCCCCCCEEEECCCCEEEEECCCCCCHHHHCCCCEEEECCHH
AILNNLEYDHADIFRNLEEIEQQFHHLVRVVPGKGLIVSNGQEENLERVLRKGCWTPVER
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHCCCCCHHH
IGVANGWEADVLTDGAVAVSFLGESQGTLLWELLGKHNRMNALAALAAARHAGVAVKAGI
CCCCCCCCCCEECCCCEEEEEECCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHH
AALAEFRNVKRRMEVKGITRDITVYDDFAHHPTAIRTTIDGLRSRVKGARIIAVLEPRSN
HHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCC
TMKMGVWKESLADSLTGADVVFCYAANLGWDLPQALLPLGEKAVAFEELDQLIAAITAYA
CEEECCHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHC
QPGDHVLIMSNAGFGGIHEKLLTRMAAVAADEVASG
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]