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The map label for this gene is sbp [H]

Identifier: 82701641

GI number: 82701641

Start: 572128

End: 573120

Strand: Reverse

Name: sbp [H]

Synonym: Nmul_A0507

Alternate gene names: 82701641

Gene position: 573120-572128 (Counterclockwise)

Preceding gene: 82701642

Following gene: 82701640

Centisome position: 18.0

GC content: 54.98

Gene sequence:

>993_bases
ATGAAAGCATGGATAACGACGAGCCTTACCGCAATCCTCCTGTGGGGAGGCAATGCCTGGGGAGATAAAACAATATTGAA
TGTATCCTATGACCCGACTCGCGAGCTGTATCAGCAGTTCAATTCGGCATTCGCGAAGCATTGGGAGAGCAAGACCGGAG
AAAAAATAGCCATAAAACAATCTCATGGCGGCTCCGGGAAACAGGCACGCTCTGTCATCGACGGGCTTGATGCTGATGTC
GTGACGCTCGCGCTGGCGAAGGACATCGATGAAATCGCGGAAAAAGCCAGCCTGCTGCCGGCGGACTGGCAAAAGCGCTT
GCCGCATAACAGCACGCCTTACACTTCAACCATCGTACTGCTGGTCCGGAAGGGCAACCCCAAGAACATCAAAGACTGGG
ACGACCTGGTCAAGCCCGGCGTCGCCGTAATCACCCCCAATCCGAAAACCTCGGGGGGCGCCCGCTGGAATTACCTCGCC
GCGTGGGAATACGGCAAGCGAACGTACGGCGACGATGCCAAGGCGAAGGAGTTCGTAGCAAAGCTCTACCGGAATGTCCC
TGTGCTGGACTCGGGCGCGCGCGGCTCAACCACGACATTTGTGGAGCGCGGCATCGGCGATGTCTTCATTTCGTGGGAAA
ACGAAGCATTCCTCGCTATCAGGGAACTCGGGCCCGACAAATTTGAAATTGTGGCACCCTCCCTCAGCATTCTTGCCGAA
CCATCGGTCGCGGTGGTAGACAAGGTGGCCGACAAGAAGGGTACCCGTACCATCGCGGAAGCCTATCTCCAATATCTCTA
TTCAGACACAGGCCAGGAGATTGCCGCTAAGAATTTCTACCGTCCAACCAATGCCGCAATCGCAGCGAAATATGCAAGTC
AGTTTCCCGGCCTCAAGCTGTTCAAGATCGACGATGCGTTTGGGGGCTGGAAGAACGCGCACAAGCTTCACTTTGCCGAT
GGCGGCACTTTTGATCAGATTTATCAGAAATAA

Upstream 100 bases:

>100_bases
GGACTTTTTTTACGTTGAAGTTGTATTGAGCGCATTTTGATTATTTGACACTTATTGATTTCACTTACTGGCTTCACCTG
CCTAAAGGAAGAATACGAAA

Downstream 100 bases:

>100_bases
CAGCACAAGTCGTACCGTCTGCACTGAAAACCGCAAAACAACCATGCTGACATTCAGAAAATCGACGTCCAGGAAATTTT
TGAAAGGAGATGAAAAGTGA

Product: thiosulfate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 330; Mature: 330

Protein sequence:

>330_residues
MKAWITTSLTAILLWGGNAWGDKTILNVSYDPTRELYQQFNSAFAKHWESKTGEKIAIKQSHGGSGKQARSVIDGLDADV
VTLALAKDIDEIAEKASLLPADWQKRLPHNSTPYTSTIVLLVRKGNPKNIKDWDDLVKPGVAVITPNPKTSGGARWNYLA
AWEYGKRTYGDDAKAKEFVAKLYRNVPVLDSGARGSTTTFVERGIGDVFISWENEAFLAIRELGPDKFEIVAPSLSILAE
PSVAVVDKVADKKGTRTIAEAYLQYLYSDTGQEIAAKNFYRPTNAAIAAKYASQFPGLKLFKIDDAFGGWKNAHKLHFAD
GGTFDQIYQK

Sequences:

>Translated_330_residues
MKAWITTSLTAILLWGGNAWGDKTILNVSYDPTRELYQQFNSAFAKHWESKTGEKIAIKQSHGGSGKQARSVIDGLDADV
VTLALAKDIDEIAEKASLLPADWQKRLPHNSTPYTSTIVLLVRKGNPKNIKDWDDLVKPGVAVITPNPKTSGGARWNYLA
AWEYGKRTYGDDAKAKEFVAKLYRNVPVLDSGARGSTTTFVERGIGDVFISWENEAFLAIRELGPDKFEIVAPSLSILAE
PSVAVVDKVADKKGTRTIAEAYLQYLYSDTGQEIAAKNFYRPTNAAIAAKYASQFPGLKLFKIDDAFGGWKNAHKLHFAD
GGTFDQIYQK
>Mature_330_residues
MKAWITTSLTAILLWGGNAWGDKTILNVSYDPTRELYQQFNSAFAKHWESKTGEKIAIKQSHGGSGKQARSVIDGLDADV
VTLALAKDIDEIAEKASLLPADWQKRLPHNSTPYTSTIVLLVRKGNPKNIKDWDDLVKPGVAVITPNPKTSGGARWNYLA
AWEYGKRTYGDDAKAKEFVAKLYRNVPVLDSGARGSTTTFVERGIGDVFISWENEAFLAIRELGPDKFEIVAPSLSILAE
PSVAVVDKVADKKGTRTIAEAYLQYLYSDTGQEIAAKNFYRPTNAAIAAKYASQFPGLKLFKIDDAFGGWKNAHKLHFAD
GGTFDQIYQK

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=310, Percent_Identity=69.0322580645161, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI1788765, Length=305, Percent_Identity=47.5409836065574, Blast_Score=289, Evalue=2e-79,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36394; Mature: 36394

Theoretical pI: Translated: 9.37; Mature: 9.37

Prosite motif: PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAWITTSLTAILLWGGNAWGDKTILNVSYDPTRELYQQFNSAFAKHWESKTGEKIAIKQ
CCCEEEECEEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEE
SHGGSGKQARSVIDGLDADVVTLALAKDIDEIAEKASLLPADWQKRLPHNSTPYTSTIVL
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCEEEEE
LVRKGNPKNIKDWDDLVKPGVAVITPNPKTSGGARWNYLAAWEYGKRTYGDDAKAKEFVA
EEECCCCCCCCCHHHHHCCCEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHH
KLYRNVPVLDSGARGSTTTFVERGIGDVFISWENEAFLAIRELGPDKFEIVAPSLSILAE
HHHHCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCEEEEEECCCCCCEEEECCCHHHHCC
PSVAVVDKVADKKGTRTIAEAYLQYLYSDTGQEIAAKNFYRPTNAAIAAKYASQFPGLKL
CCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHCCCEEE
FKIDDAFGGWKNAHKLHFADGGTFDQIYQK
EEECCCCCCCCCCCEEEECCCCCHHHHHCC
>Mature Secondary Structure
MKAWITTSLTAILLWGGNAWGDKTILNVSYDPTRELYQQFNSAFAKHWESKTGEKIAIKQ
CCCEEEECEEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEEEEE
SHGGSGKQARSVIDGLDADVVTLALAKDIDEIAEKASLLPADWQKRLPHNSTPYTSTIVL
CCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCCCEEEEE
LVRKGNPKNIKDWDDLVKPGVAVITPNPKTSGGARWNYLAAWEYGKRTYGDDAKAKEFVA
EEECCCCCCCCCHHHHHCCCEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHH
KLYRNVPVLDSGARGSTTTFVERGIGDVFISWENEAFLAIRELGPDKFEIVAPSLSILAE
HHHHCCCCCCCCCCCCHHHHHHCCCCEEEEEECCCEEEEEECCCCCCEEEECCCHHHHCC
PSVAVVDKVADKKGTRTIAEAYLQYLYSDTGQEIAAKNFYRPTNAAIAAKYASQFPGLKL
CCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHCCCEEE
FKIDDAFGGWKNAHKLHFADGGTFDQIYQK
EEECCCCCCCCCCCEEEECCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]