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The map label for this gene is cysU [H]

Identifier: 82701639

GI number: 82701639

Start: 570300

End: 571136

Strand: Reverse

Name: cysU [H]

Synonym: Nmul_A0505

Alternate gene names: 82701639

Gene position: 571136-570300 (Counterclockwise)

Preceding gene: 82701640

Following gene: 82701638

Centisome position: 17.94

GC content: 55.2

Gene sequence:

>837_bases
ATGGGCACGTTCAGGCAACACAGCGTTCTGCCCGGATTCAATCTGGCGCTTGGATTTACGTTGCTTTATCTGAGCCTGAT
TGTTCTGATTCCAATCTCGGCAGCATTTGTTCGCACTGCAGAACTCACCTTGCCCGAATTCTGGGATGTGGTGACGACAC
CAAGGGTATTGGCTTCCTATCGGTTGACATTTGGGGCCTCATTCGCAGCGGCGCTGCTCAATGCGATATTCGGGCTCCTG
GTTGCGTGGGTGCTGGTGCGTTACCACTTTCCAGGAAAGAAGCTGATCGATGCGCTGGTAGACCTTCCATTTGCCCTGCC
CACCGCGGTAGCCGGTATTGCCCTGACAGCGCTCTATGCGGGGAACGGGTGGTTCGGCCAGTATCTGGAGCCGGTTGGCC
TCAAGGTTGCCTTTACGCCAGCAGGGGTCTTCGTGGCGCTGACGTTTATCGGATTGCCATTTGTAGTGCGAACGGTGCAA
CCTGTGCTGGAAGACCTCGAACCTGAACTGGAAGAAGCAGCAGCTACGCTCGGCGCGACCCGCTTGCACACATTCCTGCG
AATCATTTTTCCCGCGATTTTTCCGGCATTGATAACCGGCTTTGCGCTTGCCTTTGCCCGTGCGATCGGGGAATACGGGT
CCGTTATTTTTATTGCGGGCAACATGCCGATGATTTCCGAAATCACGCCGCTCCTGATCATTACCAAGCTCGAGCAGTAC
GACTATGCAGGCGCTACGGCGCTCGCCGTGGTCATGCTCGTGATTTCTTTCGTGTTGCTCCTGATCATTAACCTGCTGCA
ATGGTGGAGTCGCAGCCGGAGTTCATCGACAGTATGA

Upstream 100 bases:

>100_bases
TTGTTCATACCTGCTGCAACGACAGAGTACCGAAGCGCAGAGGCTTCCTCCCGGCGCATCCGGACGCTGCAGCACTATAT
CGACGCGGCGAGAATCTGAA

Downstream 100 bases:

>100_bases
GCATGAGGATATCCTGATGACTGCTATTGCTCTTCCGTTGCCCGTCCCTTCAGGCCGGGTGCGGCCCGTCGAGGAATCCG
CGTGGGTGCGGCGGTCCCTG

Product: sulfate ABC transporter, permease CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MGTFRQHSVLPGFNLALGFTLLYLSLIVLIPISAAFVRTAELTLPEFWDVVTTPRVLASYRLTFGASFAAALLNAIFGLL
VAWVLVRYHFPGKKLIDALVDLPFALPTAVAGIALTALYAGNGWFGQYLEPVGLKVAFTPAGVFVALTFIGLPFVVRTVQ
PVLEDLEPELEEAAATLGATRLHTFLRIIFPAIFPALITGFALAFARAIGEYGSVIFIAGNMPMISEITPLLIITKLEQY
DYAGATALAVVMLVISFVLLLIINLLQWWSRSRSSSTV

Sequences:

>Translated_278_residues
MGTFRQHSVLPGFNLALGFTLLYLSLIVLIPISAAFVRTAELTLPEFWDVVTTPRVLASYRLTFGASFAAALLNAIFGLL
VAWVLVRYHFPGKKLIDALVDLPFALPTAVAGIALTALYAGNGWFGQYLEPVGLKVAFTPAGVFVALTFIGLPFVVRTVQ
PVLEDLEPELEEAAATLGATRLHTFLRIIFPAIFPALITGFALAFARAIGEYGSVIFIAGNMPMISEITPLLIITKLEQY
DYAGATALAVVMLVISFVLLLIINLLQWWSRSRSSSTV
>Mature_277_residues
GTFRQHSVLPGFNLALGFTLLYLSLIVLIPISAAFVRTAELTLPEFWDVVTTPRVLASYRLTFGASFAAALLNAIFGLLV
AWVLVRYHFPGKKLIDALVDLPFALPTAVAGIALTALYAGNGWFGQYLEPVGLKVAFTPAGVFVALTFIGLPFVVRTVQP
VLEDLEPELEEAAATLGATRLHTFLRIIFPAIFPALITGFALAFARAIGEYGSVIFIAGNMPMISEITPLLIITKLEQYD
YAGATALAVVMLVISFVLLLIINLLQWWSRSRSSSTV

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=262, Percent_Identity=50.763358778626, Blast_Score=258, Evalue=4e-70,
Organism=Escherichia coli, GI87082099, Length=252, Percent_Identity=32.1428571428571, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1786980, Length=215, Percent_Identity=34.8837209302326, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1786254, Length=211, Percent_Identity=28.9099526066351, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787369, Length=216, Percent_Identity=25.9259259259259, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787368, Length=212, Percent_Identity=30.188679245283, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30164; Mature: 30033

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTFRQHSVLPGFNLALGFTLLYLSLIVLIPISAAFVRTAELTLPEFWDVVTTPRVLASY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHH
RLTFGASFAAALLNAIFGLLVAWVLVRYHFPGKKLIDALVDLPFALPTAVAGIALTALYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHC
GNGWFGQYLEPVGLKVAFTPAGVFVALTFIGLPFVVRTVQPVLEDLEPELEEAAATLGAT
CCCCHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
RLHTFLRIIFPAIFPALITGFALAFARAIGEYGSVIFIAGNMPMISEITPLLIITKLEQY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHH
DYAGATALAVVMLVISFVLLLIINLLQWWSRSRSSSTV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
GTFRQHSVLPGFNLALGFTLLYLSLIVLIPISAAFVRTAELTLPEFWDVVTTPRVLASY
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCHHHHHHH
RLTFGASFAAALLNAIFGLLVAWVLVRYHFPGKKLIDALVDLPFALPTAVAGIALTALYA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHC
GNGWFGQYLEPVGLKVAFTPAGVFVALTFIGLPFVVRTVQPVLEDLEPELEEAAATLGAT
CCCCHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
RLHTFLRIIFPAIFPALITGFALAFARAIGEYGSVIFIAGNMPMISEITPLLIITKLEQY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHH
DYAGATALAVVMLVISFVLLLIINLLQWWSRSRSSSTV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]