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The map label for this gene is cysW [H]

Identifier: 82701638

GI number: 82701638

Start: 569402

End: 570283

Strand: Reverse

Name: cysW [H]

Synonym: Nmul_A0504

Alternate gene names: 82701638

Gene position: 570283-569402 (Counterclockwise)

Preceding gene: 82701639

Following gene: 82701637

Centisome position: 17.91

GC content: 55.33

Gene sequence:

>882_bases
ATGACTGCTATTGCTCTTCCGTTGCCCGTCCCTTCAGGCCGGGTGCGGCCCGTCGAGGAATCCGCGTGGGTGCGGCGGTC
CCTGATCGGGCTTGCGCTGGCCTTTCTTACACTCTTTCTCTTCGTACCCCTGATATCGGTTTTCTACGAAGCGCTCAAGA
AAGGCTGGGATGTTTACATTGCCGCAATTACCGAGCCGGATGCGGTGTCCGCCATTCAGCTTACCCTGACCGCAGCCGCC
ATTGCAGTTCCGCTTAATCTGGTATTCGGCATAGCGGCAGCATGGGCAGTTGCCAAGTTTGAGTTCCGCGGCAAAAACCT
CCTGGTCACGCTCATCGATTTGCCCTTTTCGGTCTCACCTGTCGTGTCCGGGCTCATCTATGTGCTTATTTTCGGCTTGC
AGGGTTGGCTGGGCCCGTGGCTGGCGGAACACGACATCAAGATTATCTTTGCGGTGCCCGGTATTGTGCTGGCGACCATA
TTCGTCACGGTTCCCTTTATCGCACGCGAACTGATTCCCCTGATGCAATCCCAAGGAGCCGAAGAGGAAGAAGCGGCCGT
TGTACTCGGGGCGAACGGCTGGCAGATATTCTTCCGTGTAACGCTTCCCAATATTAAATGGGGCCTGCTTTACGGCGTCA
TCCTTTGCAATGCGCGGGCAATGGGAGAGTTTGGTGCAGTATCGGTGGTATCCGGGCATATCCGCGGGAGCACCAATACC
TTGCCGCTGCATGTCGAGATTCTTTATAACGAATATAACTTCGCGGCCGCCTTTGCCGTGGCGTCGCTGCTCGCCCTGCT
CGCACTGGTCACATTGGCATTGAAGACTATCATTGAATCAAAAACCCGGGAAAAACCGGAAACCGCAGGAATACAAGAAT
GA

Upstream 100 bases:

>100_bases
ATGCTCGTGATTTCTTTCGTGTTGCTCCTGATCATTAACCTGCTGCAATGGTGGAGTCGCAGCCGGAGTTCATCGACAGT
ATGAGCATGAGGATATCCTG

Downstream 100 bases:

>100_bases
GCATAGAAATTCGTAACCTTTCCAAACAATTCGGCACGTTTTCCGCGCTACGCGACGTCAGCCTGGAAGTAAAGTCCGGT
GAACTGCTCGCGCTGCTCGG

Product: sulfate ABC transporter, permease CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MTAIALPLPVPSGRVRPVEESAWVRRSLIGLALAFLTLFLFVPLISVFYEALKKGWDVYIAAITEPDAVSAIQLTLTAAA
IAVPLNLVFGIAAAWAVAKFEFRGKNLLVTLIDLPFSVSPVVSGLIYVLIFGLQGWLGPWLAEHDIKIIFAVPGIVLATI
FVTVPFIARELIPLMQSQGAEEEEAAVVLGANGWQIFFRVTLPNIKWGLLYGVILCNARAMGEFGAVSVVSGHIRGSTNT
LPLHVEILYNEYNFAAAFAVASLLALLALVTLALKTIIESKTREKPETAGIQE

Sequences:

>Translated_293_residues
MTAIALPLPVPSGRVRPVEESAWVRRSLIGLALAFLTLFLFVPLISVFYEALKKGWDVYIAAITEPDAVSAIQLTLTAAA
IAVPLNLVFGIAAAWAVAKFEFRGKNLLVTLIDLPFSVSPVVSGLIYVLIFGLQGWLGPWLAEHDIKIIFAVPGIVLATI
FVTVPFIARELIPLMQSQGAEEEEAAVVLGANGWQIFFRVTLPNIKWGLLYGVILCNARAMGEFGAVSVVSGHIRGSTNT
LPLHVEILYNEYNFAAAFAVASLLALLALVTLALKTIIESKTREKPETAGIQE
>Mature_292_residues
TAIALPLPVPSGRVRPVEESAWVRRSLIGLALAFLTLFLFVPLISVFYEALKKGWDVYIAAITEPDAVSAIQLTLTAAAI
AVPLNLVFGIAAAWAVAKFEFRGKNLLVTLIDLPFSVSPVVSGLIYVLIFGLQGWLGPWLAEHDIKIIFAVPGIVLATIF
VTVPFIARELIPLMQSQGAEEEEAAVVLGANGWQIFFRVTLPNIKWGLLYGVILCNARAMGEFGAVSVVSGHIRGSTNTL
PLHVEILYNEYNFAAAFAVASLLALLALVTLALKTIIESKTREKPETAGIQE

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=278, Percent_Identity=52.158273381295, Blast_Score=296, Evalue=1e-81,
Organism=Escherichia coli, GI1788764, Length=229, Percent_Identity=37.117903930131, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI1786980, Length=225, Percent_Identity=31.1111111111111, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31651; Mature: 31520

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIALPLPVPSGRVRPVEESAWVRRSLIGLALAFLTLFLFVPLISVFYEALKKGWDVYI
CCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
AAITEPDAVSAIQLTLTAAAIAVPLNLVFGIAAAWAVAKFEFRGKNLLVTLIDLPFSVSP
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEEECCCCHHH
VVSGLIYVLIFGLQGWLGPWLAEHDIKIIFAVPGIVLATIFVTVPFIARELIPLMQSQGA
HHHHHHHHHHHHHHHHHCCHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EEEEAAVVLGANGWQIFFRVTLPNIKWGLLYGVILCNARAMGEFGAVSVVSGHIRGSTNT
CCCCCEEEEECCCEEEEEEEECCCCHHHHHHHHHHHCCHHCCCCCCEEEEEEEECCCCCC
LPLHVEILYNEYNFAAAFAVASLLALLALVTLALKTIIESKTREKPETAGIQE
EEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TAIALPLPVPSGRVRPVEESAWVRRSLIGLALAFLTLFLFVPLISVFYEALKKGWDVYI
CEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
AAITEPDAVSAIQLTLTAAAIAVPLNLVFGIAAAWAVAKFEFRGKNLLVTLIDLPFSVSP
EEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEEEECCCCHHH
VVSGLIYVLIFGLQGWLGPWLAEHDIKIIFAVPGIVLATIFVTVPFIARELIPLMQSQGA
HHHHHHHHHHHHHHHHHCCHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EEEEAAVVLGANGWQIFFRVTLPNIKWGLLYGVILCNARAMGEFGAVSVVSGHIRGSTNT
CCCCCEEEEECCCEEEEEEEECCCCHHHHHHHHHHHCCHHCCCCCCEEEEEEEECCCCCC
LPLHVEILYNEYNFAAAFAVASLLALLALVTLALKTIIESKTREKPETAGIQE
EEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]