Click here to switch to the map view.

The map label for this gene is rpoS [H]

Identifier: 82701632

GI number: 82701632

Start: 556677

End: 557630

Strand: Direct

Name: rpoS [H]

Synonym: Nmul_A0498

Alternate gene names: 82701632

Gene position: 556677-557630 (Clockwise)

Preceding gene: 82701631

Following gene: 82701633

Centisome position: 17.48

GC content: 50.63

Gene sequence:

>954_bases
ATGATGAACCAGCAAGAATACACTGACGAAGAAGAACTCTTCGAGAAGGAGGATTCACTGGAGCAGGATGATGAGGCTGC
CGACACGCCCTCCAGCGCAATAACGGACACATCCAACGACTTTCTATTGACCGATGTTACTCAAATCTATTTCAACGAGA
TTGGCCATAATGCCCTGCTGACCCCGAAGGAAGAAGCGGAACTGACGCGCCGCGTCAGACAGAACGATTTTGCCGCACGT
CAGAAAATGATTGAGTGTAATTTGCGACTGGTGGTCAATATAGCAAAACATTATACCAACCATGGGGTTGCCTTGCTCGA
CCTGATAGAAGAAGGCAACATGGGGATGATGCACGCTCTTGATAAATTCGATCCGGAACGAGGATTCCGTTTTTCGACCT
ATGCGACTTGGTGGATACGACAGAATATCGAGCGTTGCATCATGAATCAGTCGCGGACTATCCGTCTTCCGGTCCATGTC
ATCAAGGATTTGAACATCATATTGCAGGCCGCCCGCCACCTTGAAGCCCATACGGGAAAAGACCCCAAAGCGGAGGATGT
GGCTCATCTGCTGGGGCGGACAGTCAAGGAAGTGCAAAGAATGTTGTCTTTGAACCAGAGCATGATGTCATTGGACGCGC
CGCTCGATGTGGATCCCTTGCTGTCAATCGGTGATGCAATTGCAGATGACCAGAGTCCCGGCCCGGATCTTATGTGGGAG
CAGTCCGAGATAAAAGGTCGCGTACAGGATTGGTTGAACCGGTTAAATGATAAACAGCGCTATATAATCGAGCAGCGCTA
CGGGCTCAACGGCTATGAAACGCAAACTCTGGAGCAACTGGCTGCAAGTCTCGATCTTACACGCGAACGCGTGCGCCAGA
TTCAACTGGAAGCGCTGCAAACCTTGCGCATGATACTGAGGTGCGAGGGCGTATCGAAGGAGGGAGTATTTTGA

Upstream 100 bases:

>100_bases
CGGTCTTGTCAAGCTCCATTTCGAAATTCGGAAGAATGGCAAACCGGTCGATCCTCTGAAATATTTACCGGGTATTTCCG
GATGAATTGAGGATGAGCTC

Downstream 100 bases:

>100_bases
GGAGCCGCTGACCAAATCTTCCATAACGCGCGTTGCGCCTCCGGGGATTTATTCAGGGGCTCCTCAAGTTTTCAACTTCC
TGGAGTTTTCAACCCCCAGG

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MMNQQEYTDEEELFEKEDSLEQDDEAADTPSSAITDTSNDFLLTDVTQIYFNEIGHNALLTPKEEAELTRRVRQNDFAAR
QKMIECNLRLVVNIAKHYTNHGVALLDLIEEGNMGMMHALDKFDPERGFRFSTYATWWIRQNIERCIMNQSRTIRLPVHV
IKDLNIILQAARHLEAHTGKDPKAEDVAHLLGRTVKEVQRMLSLNQSMMSLDAPLDVDPLLSIGDAIADDQSPGPDLMWE
QSEIKGRVQDWLNRLNDKQRYIIEQRYGLNGYETQTLEQLAASLDLTRERVRQIQLEALQTLRMILRCEGVSKEGVF

Sequences:

>Translated_317_residues
MMNQQEYTDEEELFEKEDSLEQDDEAADTPSSAITDTSNDFLLTDVTQIYFNEIGHNALLTPKEEAELTRRVRQNDFAAR
QKMIECNLRLVVNIAKHYTNHGVALLDLIEEGNMGMMHALDKFDPERGFRFSTYATWWIRQNIERCIMNQSRTIRLPVHV
IKDLNIILQAARHLEAHTGKDPKAEDVAHLLGRTVKEVQRMLSLNQSMMSLDAPLDVDPLLSIGDAIADDQSPGPDLMWE
QSEIKGRVQDWLNRLNDKQRYIIEQRYGLNGYETQTLEQLAASLDLTRERVRQIQLEALQTLRMILRCEGVSKEGVF
>Mature_317_residues
MMNQQEYTDEEELFEKEDSLEQDDEAADTPSSAITDTSNDFLLTDVTQIYFNEIGHNALLTPKEEAELTRRVRQNDFAAR
QKMIECNLRLVVNIAKHYTNHGVALLDLIEEGNMGMMHALDKFDPERGFRFSTYATWWIRQNIERCIMNQSRTIRLPVHV
IKDLNIILQAARHLEAHTGKDPKAEDVAHLLGRTVKEVQRMLSLNQSMMSLDAPLDVDPLLSIGDAIADDQSPGPDLMWE
QSEIKGRVQDWLNRLNDKQRYIIEQRYGLNGYETQTLEQLAASLDLTRERVRQIQLEALQTLRMILRCEGVSKEGVF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in acid resistance [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=306, Percent_Identity=48.0392156862745, Blast_Score=301, Evalue=5e-83,
Organism=Escherichia coli, GI1789448, Length=253, Percent_Identity=37.5494071146245, Blast_Score=184, Evalue=6e-48,
Organism=Escherichia coli, GI1789871, Length=290, Percent_Identity=27.5862068965517, Blast_Score=98, Evalue=8e-22,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 36545; Mature: 36545

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMNQQEYTDEEELFEKEDSLEQDDEAADTPSSAITDTSNDFLLTDVTQIYFNEIGHNALL
CCCCCCCCCHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCC
TPKEEAELTRRVRQNDFAARQKMIECNLRLVVNIAKHYTNHGVALLDLIEEGNMGMMHAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHH
DKFDPERGFRFSTYATWWIRQNIERCIMNQSRTIRLPVHVIKDLNIILQAARHLEAHTGK
HHCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCC
DPKAEDVAHLLGRTVKEVQRMLSLNQSMMSLDAPLDVDPLLSIGDAIADDQSPGPDLMWE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCC
QSEIKGRVQDWLNRLNDKQRYIIEQRYGLNGYETQTLEQLAASLDLTRERVRQIQLEALQ
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLRMILRCEGVSKEGVF
HHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MMNQQEYTDEEELFEKEDSLEQDDEAADTPSSAITDTSNDFLLTDVTQIYFNEIGHNALL
CCCCCCCCCHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCC
TPKEEAELTRRVRQNDFAARQKMIECNLRLVVNIAKHYTNHGVALLDLIEEGNMGMMHAL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHH
DKFDPERGFRFSTYATWWIRQNIERCIMNQSRTIRLPVHVIKDLNIILQAARHLEAHTGK
HHCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCC
DPKAEDVAHLLGRTVKEVQRMLSLNQSMMSLDAPLDVDPLLSIGDAIADDQSPGPDLMWE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCC
QSEIKGRVQDWLNRLNDKQRYIIEQRYGLNGYETQTLEQLAASLDLTRERVRQIQLEALQ
HHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TLRMILRCEGVSKEGVF
HHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7959068; 10984043 [H]