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The map label for this gene is pcm2

Identifier: 82701630

GI number: 82701630

Start: 554904

End: 555539

Strand: Direct

Name: pcm2

Synonym: Nmul_A0496

Alternate gene names: 82701630

Gene position: 554904-555539 (Clockwise)

Preceding gene: 82701629

Following gene: 82701631

Centisome position: 17.43

GC content: 56.6

Gene sequence:

>636_bases
ATGACTTCACAGCGGACGCGTGCGCGCATGGTTGAGCGACTGTGCACGCAGGGGGTCACGGATGAAGCAGTGCTCACAGC
CATGATTACCGTTCCTCGTCACATCTTTGTGGAGGAAGTGCTGGCAAGCCGTGCCTACGACGACGTCGCCTTGCCGATCA
ATTTCGAGCAGACGATTTCGAACCCGTTCACCGTGGCGAGGATGAGTCAGTTGCTGCGCGCAGGTTCCAATCCGGGCAAG
GTGCTTGAGATTGGCACGGGCTCCGGCTATCAGACTGCGATACTGGCGCAGTTCTCCCGGGAAGTCTATTCGGTCGAGCG
TATCGGGCCTCTGCTGACGCGCACTCGCGGGCGGCTAAGAGAGTTACGCATCAACAATGTGCGCCTGAAGCATGCTGACG
GTCTGCTTGGCCTTTCCGAGGCAGCGCCGTTTGACGGAATCATTCTCACGGCTGCAACGCCGCACATGCCTCCGGCACTA
CTTGAACAATTAGCCATTCGTGGCAGAATGGTATTTCCCAGGGGAACCAGCGAGCAAGTTTTGTGCGTTATCGAACGCAA
CTTGCAAGGTTATACTGAGACAATACTGGACGAAGTCAAGTTTGTCCCAATGATACCGGGGATCTGGCAGCGTTAG

Upstream 100 bases:

>100_bases
GTAAAGGAATGGCTGAATAGTCACGCTCTTGGCAGCACGCGGAGCTGAAGGCTGTGTTCAAGGCAGATCGGGATTGAACG
TCCGCCATTCCGGAATCGGC

Downstream 100 bases:

>100_bases
GAAACCGCAACGATATCGAGCAAGGGAAACGAGACAACAATGAGCGCTACACGAATCAGGGATATGAGTCTGAACAGTAA
TCTTTCCGGTCTGGGCAAAG

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase 2; Protein L-isoaspartyl methyltransferase 2; Protein-beta-aspartate methyltransferase 2; PIMT 2

Number of amino acids: Translated: 211; Mature: 210

Protein sequence:

>211_residues
MTSQRTRARMVERLCTQGVTDEAVLTAMITVPRHIFVEEVLASRAYDDVALPINFEQTISNPFTVARMSQLLRAGSNPGK
VLEIGTGSGYQTAILAQFSREVYSVERIGPLLTRTRGRLRELRINNVRLKHADGLLGLSEAAPFDGIILTAATPHMPPAL
LEQLAIRGRMVFPRGTSEQVLCVIERNLQGYTETILDEVKFVPMIPGIWQR

Sequences:

>Translated_211_residues
MTSQRTRARMVERLCTQGVTDEAVLTAMITVPRHIFVEEVLASRAYDDVALPINFEQTISNPFTVARMSQLLRAGSNPGK
VLEIGTGSGYQTAILAQFSREVYSVERIGPLLTRTRGRLRELRINNVRLKHADGLLGLSEAAPFDGIILTAATPHMPPAL
LEQLAIRGRMVFPRGTSEQVLCVIERNLQGYTETILDEVKFVPMIPGIWQR
>Mature_210_residues
TSQRTRARMVERLCTQGVTDEAVLTAMITVPRHIFVEEVLASRAYDDVALPINFEQTISNPFTVARMSQLLRAGSNPGKV
LEIGTGSGYQTAILAQFSREVYSVERIGPLLTRTRGRLRELRINNVRLKHADGLLGLSEAAPFDGIILTAATPHMPPALL
EQLAIRGRMVFPRGTSEQVLCVIERNLQGYTETILDEVKFVPMIPGIWQR

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=180, Percent_Identity=29.4444444444444, Blast_Score=70, Evalue=9e-13,
Organism=Escherichia coli, GI1789100, Length=207, Percent_Identity=45.8937198067633, Blast_Score=173, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI71983477, Length=219, Percent_Identity=29.2237442922374, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI17981723, Length=213, Percent_Identity=28.169014084507, Blast_Score=67, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT2_NITMU (Q2YBR7)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411196.1
- ProteinModelPortal:   Q2YBR7
- SMR:   Q2YBR7
- STRING:   Q2YBR7
- GeneID:   3784859
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0496
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   YSVERIR
- PhylomeDB:   Q2YBR7
- ProtClustDB:   CLSK585560
- BioCyc:   NMUL323848:NMUL_A0496-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 23486; Mature: 23354

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSQRTRARMVERLCTQGVTDEAVLTAMITVPRHIFVEEVLASRAYDDVALPINFEQTIS
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHC
NPFTVARMSQLLRAGSNPGKVLEIGTGSGYQTAILAQFSREVYSVERIGPLLTRTRGRLR
CCHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELRINNVRLKHADGLLGLSEAAPFDGIILTAATPHMPPALLEQLAIRGRMVFPRGTSEQV
HHHHCCEEEECCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCEEECCCCCCCCE
LCVIERNLQGYTETILDEVKFVPMIPGIWQR
EEEEHHCCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TSQRTRARMVERLCTQGVTDEAVLTAMITVPRHIFVEEVLASRAYDDVALPINFEQTIS
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHC
NPFTVARMSQLLRAGSNPGKVLEIGTGSGYQTAILAQFSREVYSVERIGPLLTRTRGRLR
CCHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELRINNVRLKHADGLLGLSEAAPFDGIILTAATPHMPPALLEQLAIRGRMVFPRGTSEQV
HHHHCCEEEECCCCCCCCCCCCCCCCEEEECCCCCCCHHHHHHHHHCCEEECCCCCCCCE
LCVIERNLQGYTETILDEVKFVPMIPGIWQR
EEEEHHCCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA