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The map label for this gene is proS

Identifier: 82701584

GI number: 82701584

Start: 499228

End: 500937

Strand: Direct

Name: proS

Synonym: Nmul_A0450

Alternate gene names: 82701584

Gene position: 499228-500937 (Clockwise)

Preceding gene: 82701573

Following gene: 82701588

Centisome position: 15.68

GC content: 54.33

Gene sequence:

>1710_bases
ATGCGAGCATCAGGTTTTTTCATTTCGACACTCAAGGAAGCCCCCGCCGAAGCGGAATTGATCAGCCACAAGCTGATGCT
GAGGGCCGGTATCATCCGGCGGCTGGGGAGCGGTCTCTACACGTGGATGCCCCTCGGCTTGAAGGTTCTGCGCAAAGTTG
AAAACATCGTGCGGGAGGAAATGGATGCCGCGGGCGCACTGGAACTGCTGATGCCGGCTGTGCAGCCTGCGGAGCTATGG
CGGGAAACAGGGCGATGGGACGTCTTTGGTCCCCAGATGTTGAAAATTAGAGACAGACACGAGCGCGATTTCTGTTTTGG
TCCAACCCATGAAGAGGTCATAACGGATATTGCACGGCGTGAAATCAAGAGTTACCGGCAGTTACCTCTCAATTTTTATC
AGATACAAACCAAATTTCGCGACGAGGTTCGCCCCCGCTTCGGCGTCATGCGCGCGCGCGAGTTCGTGATGAAAGATGCC
TATTCGTTCCACACCGATATACCCAGCCTGGAAGAGACCTATCAGGCCATGCATGTGGCCTATTGCCGGATATTCGATCG
CCTGGGGCTGAAGTTTCGTCCCGTCAAGGCCGATACGGGTGCGATTGGTGGCAGCAGTTCGCATGAATTTCATGTCCTGG
CCGATTCCGGCGAAGACGCCATCGCTTTTTGTTCCGATTCCGATTACGCAGCGAACGTTGAAATGGCCGAGTCGTTGCCG
CCAGCAGGACTGCGGGAGGCTGCGGCGGGCGAGATGCAGAAAGTGCGAACAATCGCCCAAAAGACATGCGAAGAGGTTGC
TGCCTATCTCAATGTATCCATCGAGCAGACGGTAAAAACGCTGGCGGTCATGGCCAATGGCGGAATGCATCTTTTGCTGC
TGCGTGGCGATCATCATCTCAACGAGACAAAAGTTCGAAAGATTCCTTTTCTTTCCGATTTCCGGCTTGCCAGCGAAGAA
GAAATTCGCACCGAAACAGGATGTCTTCCCGGGTTTATCGGCCCAGCCGGATTGTCTCTTCCGGTTATCGCAGACCTTAC
CGTAGCCACCATGAGTAACTTTGTGTGCGGCGCCAACGAAGAGGATTACCATCTCGTCAACGTCAATTTCGGGCGTGATC
TGAAAGAGCCGGATCATGTTTTCGATATCCGCAACGTGGTTTCCGGTGACTTGTCGCCGGACGGAAAGGGTAAGCTGGAA
ATCTGTCGCGGCATAGAAGTCGGCCATATTTTCCAATTGCTCACAAAGTATTCGGAAGCGATGAAAGCCAATTATCTCGA
TGAATCCGGGCAAGCGCGTCCCATGGAAATGGGCTGCTACGGGATCGGGGTTTCACGTATTGTGGCAGCTGCCATCGAGC
AGAACCATGACGAGCGCGGCATTATATTTCCCGCGGCAATGGCGCCATTTCAGGTAGTCATCATTCCGATCGGGTTGAAG
AAGAATGCAGAAGTGAGGGCTGAGGCGGAGAAACTATACGCGACGCTTTCCAGTGTCGGCATCGAGGTTCTGCTCGACGA
CCGGGATGACCGCCCCGGTGTCATGTTCGCCGACATGGAACTGATCGGTATTCCTCACCGGGTTGTCGTCGGCGAGCGGG
GCTTGAAGGAAGGAAATGCCGAGTATCGCGGGCGGCGTGACGAAAAATCGGAGGTCGTCCCCCTTCCCGAGATCGCAGAT
TTTATAAAATCAAAATTAGCCGGGGGTTGA

Upstream 100 bases:

>100_bases
CTGCATTATTGTTTGAACACTTTGCTTTTCAGATTCCTCATAGCCAACCTGCGCTGCTTTTCAGAGTAAAATCGGAGATT
GGACTTGTTATGGACAGAAT

Downstream 100 bases:

>100_bases
CGAGAGAACCTCCTCAGGTTTGCCGGCCTGATGGATGTTCAGCCCGACCGTAGGCCGATCCGAGGAGGGCAAGGCTGAGG
GCTGGAGAATTTTGCTCCGC

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 569; Mature: 569

Protein sequence:

>569_residues
MRASGFFISTLKEAPAEAELISHKLMLRAGIIRRLGSGLYTWMPLGLKVLRKVENIVREEMDAAGALELLMPAVQPAELW
RETGRWDVFGPQMLKIRDRHERDFCFGPTHEEVITDIARREIKSYRQLPLNFYQIQTKFRDEVRPRFGVMRAREFVMKDA
YSFHTDIPSLEETYQAMHVAYCRIFDRLGLKFRPVKADTGAIGGSSSHEFHVLADSGEDAIAFCSDSDYAANVEMAESLP
PAGLREAAAGEMQKVRTIAQKTCEEVAAYLNVSIEQTVKTLAVMANGGMHLLLLRGDHHLNETKVRKIPFLSDFRLASEE
EIRTETGCLPGFIGPAGLSLPVIADLTVATMSNFVCGANEEDYHLVNVNFGRDLKEPDHVFDIRNVVSGDLSPDGKGKLE
ICRGIEVGHIFQLLTKYSEAMKANYLDESGQARPMEMGCYGIGVSRIVAAAIEQNHDERGIIFPAAMAPFQVVIIPIGLK
KNAEVRAEAEKLYATLSSVGIEVLLDDRDDRPGVMFADMELIGIPHRVVVGERGLKEGNAEYRGRRDEKSEVVPLPEIAD
FIKSKLAGG

Sequences:

>Translated_569_residues
MRASGFFISTLKEAPAEAELISHKLMLRAGIIRRLGSGLYTWMPLGLKVLRKVENIVREEMDAAGALELLMPAVQPAELW
RETGRWDVFGPQMLKIRDRHERDFCFGPTHEEVITDIARREIKSYRQLPLNFYQIQTKFRDEVRPRFGVMRAREFVMKDA
YSFHTDIPSLEETYQAMHVAYCRIFDRLGLKFRPVKADTGAIGGSSSHEFHVLADSGEDAIAFCSDSDYAANVEMAESLP
PAGLREAAAGEMQKVRTIAQKTCEEVAAYLNVSIEQTVKTLAVMANGGMHLLLLRGDHHLNETKVRKIPFLSDFRLASEE
EIRTETGCLPGFIGPAGLSLPVIADLTVATMSNFVCGANEEDYHLVNVNFGRDLKEPDHVFDIRNVVSGDLSPDGKGKLE
ICRGIEVGHIFQLLTKYSEAMKANYLDESGQARPMEMGCYGIGVSRIVAAAIEQNHDERGIIFPAAMAPFQVVIIPIGLK
KNAEVRAEAEKLYATLSSVGIEVLLDDRDDRPGVMFADMELIGIPHRVVVGERGLKEGNAEYRGRRDEKSEVVPLPEIAD
FIKSKLAGG
>Mature_569_residues
MRASGFFISTLKEAPAEAELISHKLMLRAGIIRRLGSGLYTWMPLGLKVLRKVENIVREEMDAAGALELLMPAVQPAELW
RETGRWDVFGPQMLKIRDRHERDFCFGPTHEEVITDIARREIKSYRQLPLNFYQIQTKFRDEVRPRFGVMRAREFVMKDA
YSFHTDIPSLEETYQAMHVAYCRIFDRLGLKFRPVKADTGAIGGSSSHEFHVLADSGEDAIAFCSDSDYAANVEMAESLP
PAGLREAAAGEMQKVRTIAQKTCEEVAAYLNVSIEQTVKTLAVMANGGMHLLLLRGDHHLNETKVRKIPFLSDFRLASEE
EIRTETGCLPGFIGPAGLSLPVIADLTVATMSNFVCGANEEDYHLVNVNFGRDLKEPDHVFDIRNVVSGDLSPDGKGKLE
ICRGIEVGHIFQLLTKYSEAMKANYLDESGQARPMEMGCYGIGVSRIVAAAIEQNHDERGIIFPAAMAPFQVVIIPIGLK
KNAEVRAEAEKLYATLSSVGIEVLLDDRDDRPGVMFADMELIGIPHRVVVGERGLKEGNAEYRGRRDEKSEVVPLPEIAD
FIKSKLAGG

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily

Homologues:

Organism=Homo sapiens, GI34303926, Length=228, Percent_Identity=43.421052631579, Blast_Score=221, Evalue=2e-57,
Organism=Escherichia coli, GI1786392, Length=573, Percent_Identity=58.9877835951134, Blast_Score=697, Evalue=0.0,
Organism=Escherichia coli, GI1788013, Length=163, Percent_Identity=26.9938650306748, Blast_Score=64, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI115532348, Length=246, Percent_Identity=37.3983739837398, Blast_Score=174, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI193203271, Length=90, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320931, Length=548, Percent_Identity=30.8394160583942, Blast_Score=240, Evalue=4e-64,
Organism=Drosophila melanogaster, GI24656200, Length=238, Percent_Identity=38.655462184874, Blast_Score=184, Evalue=1e-46,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_NITMU (Q2YBW3)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411150.1
- ProteinModelPortal:   Q2YBW3
- SMR:   Q2YBW3
- STRING:   Q2YBW3
- GeneID:   3785918
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0450
- eggNOG:   COG0442
- HOGENOM:   HBG403504
- OMA:   DFVLGPT
- PhylomeDB:   Q2YBW3
- ProtClustDB:   PRK09194
- BioCyc:   NMUL323848:NMUL_A0450-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01569
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.90.960.10
- PRINTS:   PR01046
- TIGRFAMs:   TIGR00409

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK; SSF52954 Anticodon_bd; SSF55826 YbaK/aa-tRNA-synth-assoc-reg

EC number: =6.1.1.15

Molecular weight: Translated: 63313; Mature: 63313

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRASGFFISTLKEAPAEAELISHKLMLRAGIIRRLGSGLYTWMPLGLKVLRKVENIVREE
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
MDAAGALELLMPAVQPAELWRETGRWDVFGPQMLKIRDRHERDFCFGPTHEEVITDIARR
HHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
EIKSYRQLPLNFYQIQTKFRDEVRPRFGVMRAREFVMKDAYSFHTDIPSLEETYQAMHVA
HHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
YCRIFDRLGLKFRPVKADTGAIGGSSSHEFHVLADSGEDAIAFCSDSDYAANVEMAESLP
HHHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCHHHHCCC
PAGLREAAAGEMQKVRTIAQKTCEEVAAYLNVSIEQTVKTLAVMANGGMHLLLLRGDHHL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEECCCCC
NETKVRKIPFLSDFRLASEEEIRTETGCLPGFIGPAGLSLPVIADLTVATMSNFVCGANE
CCCHHHCCCCHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
EDYHLVNVNFGRDLKEPDHVFDIRNVVSGDLSPDGKGKLEICRGIEVGHIFQLLTKYSEA
CCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHH
MKANYLDESGQARPMEMGCYGIGVSRIVAAAIEQNHDERGIIFPAAMAPFQVVIIPIGLK
HHHHCCCCCCCCCCCCCCEECCCHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEECCC
KNAEVRAEAEKLYATLSSVGIEVLLDDRDDRPGVMFADMELIGIPHRVVVGERGLKEGNA
CCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEECCCEECCCCEEEECCCCCCCCCH
EYRGRRDEKSEVVPLPEIADFIKSKLAGG
HHCCCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MRASGFFISTLKEAPAEAELISHKLMLRAGIIRRLGSGLYTWMPLGLKVLRKVENIVREE
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
MDAAGALELLMPAVQPAELWRETGRWDVFGPQMLKIRDRHERDFCFGPTHEEVITDIARR
HHHHHHHHHHHCCCCHHHHHHHCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
EIKSYRQLPLNFYQIQTKFRDEVRPRFGVMRAREFVMKDAYSFHTDIPSLEETYQAMHVA
HHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
YCRIFDRLGLKFRPVKADTGAIGGSSSHEFHVLADSGEDAIAFCSDSDYAANVEMAESLP
HHHHHHHCCCEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEEECCCCCCCCCHHHHCCC
PAGLREAAAGEMQKVRTIAQKTCEEVAAYLNVSIEQTVKTLAVMANGGMHLLLLRGDHHL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEEEEEEECCCCC
NETKVRKIPFLSDFRLASEEEIRTETGCLPGFIGPAGLSLPVIADLTVATMSNFVCGANE
CCCHHHCCCCHHCCCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCC
EDYHLVNVNFGRDLKEPDHVFDIRNVVSGDLSPDGKGKLEICRGIEVGHIFQLLTKYSEA
CCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHCCCCCHHHHHHHHHHHHHH
MKANYLDESGQARPMEMGCYGIGVSRIVAAAIEQNHDERGIIFPAAMAPFQVVIIPIGLK
HHHHCCCCCCCCCCCCCCEECCCHHHHHHHHHHCCCCCCCEEEECCCCCEEEEEEEECCC
KNAEVRAEAEKLYATLSSVGIEVLLDDRDDRPGVMFADMELIGIPHRVVVGERGLKEGNA
CCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEECCCEECCCCEEEECCCCCCCCCH
EYRGRRDEKSEVVPLPEIADFIKSKLAGG
HHCCCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA