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The map label for this gene is hisZ

Identifier: 82701576

GI number: 82701576

Start: 491672

End: 492853

Strand: Reverse

Name: hisZ

Synonym: Nmul_A0442

Alternate gene names: 82701576

Gene position: 492853-491672 (Counterclockwise)

Preceding gene: 82701577

Following gene: 82701575

Centisome position: 15.48

GC content: 55.84

Gene sequence:

>1182_bases
ATGAATATGAGTGCATGGGCTCTTCCCGAATACATTGAGGACATTCTGCCCGCTGAAGCGCTGAAGATAGAAATGATGCG
CCGGCGGGTATTGGACTGGCTGTTTGTGAATGGCTACGAACTCGTGGGGCCGCCGTTGCTGGAATATGTGGAATCGCTTC
TCACTGGCAGCGGAGGGCAAATGAATTTGCGCGTACTTAAGGTGGTGGATCAACTGAGCGGCCGCATGATGGGCTTGCGC
GCGGACATGACGCCACAGGTGGCTCGCATCGATGCACACCTTCTGAACCGGAAGGGCATTACCCGACTTTGTTATGCGGG
CAGCGTGCTGCACGCACGGCCGTCGGGCCTGACTCGGACCCGGGAACCGTTGCAGATCGGTGCGGAACTTTACGGTCATC
AAGGACTTGAAAGCGATCTGGAAATCCAGCGTTTGATGCTGCAATCTCTCGCAATCGCCGGTGTGGGAAACATACACCTG
GATCTCGGGCATGTCGCGGTATTTCGCGGCTTGATCAGAAGTACAGGTATCTCCCCGGACCTGGAAATGGAATTGTCCGG
GGCCTTGCAGGGGAAGGATAAGGCAGCATTGAAGGAGCTCTGCGCCGGGCTGAAAAAACAGGTGGATGCTTCGGTGCGGG
AGGCGCTCCAGCTGCTACCCGAGCTCTACGGGGACGAGAATGTGTTGACATTGGCGCGCAGCGCCCTCCCGTCCTACCCA
GGAATCATGAAGGCGCTGGACGAACTCGAAATGGTAGCGAGCGAATTGAGCCCGCTGGTGGATACATTGGCATTCGATCT
CGCCGACTTGCGCGGATATCATTACCACAGCGGCATGGTCTTCGCCGCATATACCGACAACTGTCCCAATGCAATCGCAG
TGGGTGGACGCTACGACGAAGTTGGAAAGGCATTCGGGAGAGCGCGACCCGCAACCGGTTTCAGCATGGATCTGCGCGAG
TTATCCGGTTTGATGTCCTCGGACTCTCATCCAAGAGGAATTCTGGCCCCTTTCATAAAGGAGGACAAAGCGCTGGAAAA
GAAAATCGAACAGCTTCGTAACGAAGGCCAGATCGTGATAGTTGCCCTGCCCGGCCATGAGAATGACGCCGGCTCCTTCA
ATTGCGATAAAAAACTCGTATCGGAAAACGGTGTGTGGTCGATAGCGGATGCACTGATCTGA

Upstream 100 bases:

>100_bases
GACCTCCATGCTGGTCGGGCTGCTCCTGCTCTATCTTGTGAAATAATTCTTTCCATACCCTCCATACCCGCCTTTATCCT
GTCTTGATCACGACCCCGAC

Downstream 100 bases:

>100_bases
ATGCCAGTCCTTAGCCAGCTTTAGGAAGGAAAGTTCTTCCTCCCTTTACCTTTCAAACATTACAGAGGTTTAAACGTTAA
AGAAGCGATATGAGCAAAAA

Product: ATP phosphoribosyltransferase regulatory subunit

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MNMSAWALPEYIEDILPAEALKIEMMRRRVLDWLFVNGYELVGPPLLEYVESLLTGSGGQMNLRVLKVVDQLSGRMMGLR
ADMTPQVARIDAHLLNRKGITRLCYAGSVLHARPSGLTRTREPLQIGAELYGHQGLESDLEIQRLMLQSLAIAGVGNIHL
DLGHVAVFRGLIRSTGISPDLEMELSGALQGKDKAALKELCAGLKKQVDASVREALQLLPELYGDENVLTLARSALPSYP
GIMKALDELEMVASELSPLVDTLAFDLADLRGYHYHSGMVFAAYTDNCPNAIAVGGRYDEVGKAFGRARPATGFSMDLRE
LSGLMSSDSHPRGILAPFIKEDKALEKKIEQLRNEGQIVIVALPGHENDAGSFNCDKKLVSENGVWSIADALI

Sequences:

>Translated_393_residues
MNMSAWALPEYIEDILPAEALKIEMMRRRVLDWLFVNGYELVGPPLLEYVESLLTGSGGQMNLRVLKVVDQLSGRMMGLR
ADMTPQVARIDAHLLNRKGITRLCYAGSVLHARPSGLTRTREPLQIGAELYGHQGLESDLEIQRLMLQSLAIAGVGNIHL
DLGHVAVFRGLIRSTGISPDLEMELSGALQGKDKAALKELCAGLKKQVDASVREALQLLPELYGDENVLTLARSALPSYP
GIMKALDELEMVASELSPLVDTLAFDLADLRGYHYHSGMVFAAYTDNCPNAIAVGGRYDEVGKAFGRARPATGFSMDLRE
LSGLMSSDSHPRGILAPFIKEDKALEKKIEQLRNEGQIVIVALPGHENDAGSFNCDKKLVSENGVWSIADALI
>Mature_393_residues
MNMSAWALPEYIEDILPAEALKIEMMRRRVLDWLFVNGYELVGPPLLEYVESLLTGSGGQMNLRVLKVVDQLSGRMMGLR
ADMTPQVARIDAHLLNRKGITRLCYAGSVLHARPSGLTRTREPLQIGAELYGHQGLESDLEIQRLMLQSLAIAGVGNIHL
DLGHVAVFRGLIRSTGISPDLEMELSGALQGKDKAALKELCAGLKKQVDASVREALQLLPELYGDENVLTLARSALPSYP
GIMKALDELEMVASELSPLVDTLAFDLADLRGYHYHSGMVFAAYTDNCPNAIAVGGRYDEVGKAFGRARPATGFSMDLRE
LSGLMSSDSHPRGILAPFIKEDKALEKKIEQLRNEGQIVIVALPGHENDAGSFNCDKKLVSENGVWSIADALI

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_NITMU (Q2YBX1)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411142.1
- ProteinModelPortal:   Q2YBX1
- STRING:   Q2YBX1
- GeneID:   3785910
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0442
- eggNOG:   COG3705
- HOGENOM:   HBG616575
- OMA:   DLRGYHY
- ProtClustDB:   PRK12292
- BioCyc:   NMUL323848:NMUL_A0442-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR004517
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00443

Pfam domain/function: NA

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 42932; Mature: 42932

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMSAWALPEYIEDILPAEALKIEMMRRRVLDWLFVNGYELVGPPLLEYVESLLTGSGGQ
CCCCHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHCCCCCC
MNLRVLKVVDQLSGRMMGLRADMTPQVARIDAHLLNRKGITRLCYAGSVLHARPSGLTRT
CHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHCCHHCCCCCCCCCC
REPLQIGAELYGHQGLESDLEIQRLMLQSLAIAGVGNIHLDLGHVAVFRGLIRSTGISPD
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCC
LEMELSGALQGKDKAALKELCAGLKKQVDASVREALQLLPELYGDENVLTLARSALPSYP
CCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
GIMKALDELEMVASELSPLVDTLAFDLADLRGYHYHSGMVFAAYTDNCPNAIAVGGRYDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCEEEEEECCCCCCCEEECCCHHH
VGKAFGRARPATGFSMDLRELSGLMSSDSHPRGILAPFIKEDKALEKKIEQLRNEGQIVI
HHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
VALPGHENDAGSFNCDKKLVSENGVWSIADALI
EEECCCCCCCCCCCCHHHHHCCCCCCHHHHHCC
>Mature Secondary Structure
MNMSAWALPEYIEDILPAEALKIEMMRRRVLDWLFVNGYELVGPPLLEYVESLLTGSGGQ
CCCCHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHCCCHHHHHHHHHHCCCCCC
MNLRVLKVVDQLSGRMMGLRADMTPQVARIDAHLLNRKGITRLCYAGSVLHARPSGLTRT
CHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHCCHHCCCCCCCCCC
REPLQIGAELYGHQGLESDLEIQRLMLQSLAIAGVGNIHLDLGHVAVFRGLIRSTGISPD
CHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCC
LEMELSGALQGKDKAALKELCAGLKKQVDASVREALQLLPELYGDENVLTLARSALPSYP
CCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCC
GIMKALDELEMVASELSPLVDTLAFDLADLRGYHYHSGMVFAAYTDNCPNAIAVGGRYDE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCEEEEEECCCCCCCEEECCCHHH
VGKAFGRARPATGFSMDLRELSGLMSSDSHPRGILAPFIKEDKALEKKIEQLRNEGQIVI
HHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEE
VALPGHENDAGSFNCDKKLVSENGVWSIADALI
EEECCCCCCCCCCCCHHHHHCCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA