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The map label for this gene is purA

Identifier: 82701575

GI number: 82701575

Start: 490275

End: 491582

Strand: Reverse

Name: purA

Synonym: Nmul_A0441

Alternate gene names: 82701575

Gene position: 491582-490275 (Counterclockwise)

Preceding gene: 82701576

Following gene: 82701574

Centisome position: 15.44

GC content: 57.03

Gene sequence:

>1308_bases
ATGAGCAAAAATGTAGTTGTCATAGGAACCCAGTGGGGTGATGAGGGTAAGGGCAAGATCGTGGACTGGCTTACGGATCA
GGCGCAGGGCGTGGTGCGCTTCCAGGGAGGACACAACGCGGGTCATACGCTTGTCATTGGTGGCAAGCAGACCGTGCTAC
ACCTGATTCCTTCCGGAATTCTCCGTAAGGATGTCGCCTGCTATATCGGTAATGGCGTCGTTGTGTCGCCACAGGCGCTT
CTGGATGAAGTCGGCATGCTGGAGCGCGCGGGCATCGATGTGCTCAGCCGGCTCCGTATCAGCGAGGCTTGTCCTCTCAT
TCTACCCTGTCATGTGGCCCTGGATAACGCGCGGGAGATCGCCAGAGGATTGGGTAAAATCGGCACGACCGGGCGGGGTA
TTGGGCCGGCATATGAGGACAAGGTGGCGCGGCGCGCCGTCCGTTTGCAGGACTTGTTTCATCGTGACCGCTTCGCGGCA
AAGCTGGGCGAGATACTGGATTATCACAATTTCGTGTTGAAGAACTATTTCCAATCGCCAGTCGTCGATTTTCAGCAAAC
CATGGACGAGACGCTCTCACTGGTGGAACGCATCCGCCCCATGGTTGCCGATGTGCCGCGGCTTTTATTCGAAGCCAACC
GGGCAGGCGCGAATCTTCTGTTTGAGGGGGCGCAAGGAGCACTGCTCGACATTGATCACGGCACTTACCCGTTTGTTACG
TCCAGCAATTGCATTGCCGGCGCCGCAACAACAGGAAGTGGAATCGGCCCCCAGATGTTGCACTATGTGTTGGGGATCAC
CAAGGCCTATACCACGCGCGTGGGGGCGGGGCCCTTTCCAACCGAACTGGATGATGATGTCGGCAGGCATCTTGCCAAGC
GCGGCAATGAATTCGGGGCAACCACGGGGCGTCCGCGACGCTGCGGCTGGTTCGATGCCGCCGCCCTGAAGCGCTCCATC
CAGATCAATGGCGTCTCCGGATTGTGTGTCACGAAGCTCGATGTGATGGATGGGGTTGAAACCCTGCGGCTGGGCGTGGG
CTATAAAATGATGGGAAAAGGAGAGGAAGAAAAATTTAGTGCCATCATGCCGGTAGGTGCAGAGGAACTTGCCTCCTGCG
AACCCGTTTACGAGGAAATGCCCGGATGGAGCGGAAGCACCGTGGGTATTCGGAATTTCGAACAACTGCCCATGGCTGCG
CGCAACTATCTCAAGCGTATGGAAGAAGTGTGTGAGGTATCGATAGACATGATATCGACCGGACCGGATCGGGAAGAAAC
GATCGTGTTGCGGCACCCTTTCGAGTAG

Upstream 100 bases:

>100_bases
CACTGATCTGAATGCCAGTCCTTAGCCAGCTTTAGGAAGGAAAGTTCTTCCTCCCTTTACCTTTCAAACATTACAGAGGT
TTAAACGTTAAAGAAGCGAT

Downstream 100 bases:

>100_bases
CTGGAAAGCCTGCCGCCCGATTAGACAGAGGGACAGACGGTGCAGGAACAGAAGCCTCCGTCACACGGGCAGATTACCAG
AACCGGTCGAACCAGTATGA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSKNVVVIGTQWGDEGKGKIVDWLTDQAQGVVRFQGGHNAGHTLVIGGKQTVLHLIPSGILRKDVACYIGNGVVVSPQAL
LDEVGMLERAGIDVLSRLRISEACPLILPCHVALDNAREIARGLGKIGTTGRGIGPAYEDKVARRAVRLQDLFHRDRFAA
KLGEILDYHNFVLKNYFQSPVVDFQQTMDETLSLVERIRPMVADVPRLLFEANRAGANLLFEGAQGALLDIDHGTYPFVT
SSNCIAGAATTGSGIGPQMLHYVLGITKAYTTRVGAGPFPTELDDDVGRHLAKRGNEFGATTGRPRRCGWFDAAALKRSI
QINGVSGLCVTKLDVMDGVETLRLGVGYKMMGKGEEEKFSAIMPVGAEELASCEPVYEEMPGWSGSTVGIRNFEQLPMAA
RNYLKRMEEVCEVSIDMISTGPDREETIVLRHPFE

Sequences:

>Translated_435_residues
MSKNVVVIGTQWGDEGKGKIVDWLTDQAQGVVRFQGGHNAGHTLVIGGKQTVLHLIPSGILRKDVACYIGNGVVVSPQAL
LDEVGMLERAGIDVLSRLRISEACPLILPCHVALDNAREIARGLGKIGTTGRGIGPAYEDKVARRAVRLQDLFHRDRFAA
KLGEILDYHNFVLKNYFQSPVVDFQQTMDETLSLVERIRPMVADVPRLLFEANRAGANLLFEGAQGALLDIDHGTYPFVT
SSNCIAGAATTGSGIGPQMLHYVLGITKAYTTRVGAGPFPTELDDDVGRHLAKRGNEFGATTGRPRRCGWFDAAALKRSI
QINGVSGLCVTKLDVMDGVETLRLGVGYKMMGKGEEEKFSAIMPVGAEELASCEPVYEEMPGWSGSTVGIRNFEQLPMAA
RNYLKRMEEVCEVSIDMISTGPDREETIVLRHPFE
>Mature_434_residues
SKNVVVIGTQWGDEGKGKIVDWLTDQAQGVVRFQGGHNAGHTLVIGGKQTVLHLIPSGILRKDVACYIGNGVVVSPQALL
DEVGMLERAGIDVLSRLRISEACPLILPCHVALDNAREIARGLGKIGTTGRGIGPAYEDKVARRAVRLQDLFHRDRFAAK
LGEILDYHNFVLKNYFQSPVVDFQQTMDETLSLVERIRPMVADVPRLLFEANRAGANLLFEGAQGALLDIDHGTYPFVTS
SNCIAGAATTGSGIGPQMLHYVLGITKAYTTRVGAGPFPTELDDDVGRHLAKRGNEFGATTGRPRRCGWFDAAALKRSIQ
INGVSGLCVTKLDVMDGVETLRLGVGYKMMGKGEEEKFSAIMPVGAEELASCEPVYEEMPGWSGSTVGIRNFEQLPMAAR
NYLKRMEEVCEVSIDMISTGPDREETIVLRHPFE

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI22748717, Length=435, Percent_Identity=42.9885057471264, Blast_Score=334, Evalue=1e-91,
Organism=Homo sapiens, GI34577063, Length=433, Percent_Identity=41.3394919168591, Blast_Score=315, Evalue=5e-86,
Organism=Homo sapiens, GI40316944, Length=402, Percent_Identity=41.7910447761194, Blast_Score=296, Evalue=2e-80,
Organism=Escherichia coli, GI1790620, Length=435, Percent_Identity=60.4597701149425, Blast_Score=547, Evalue=1e-157,
Organism=Caenorhabditis elegans, GI25146553, Length=437, Percent_Identity=42.1052631578947, Blast_Score=315, Evalue=3e-86,
Organism=Caenorhabditis elegans, GI25146555, Length=437, Percent_Identity=42.1052631578947, Blast_Score=315, Evalue=3e-86,
Organism=Saccharomyces cerevisiae, GI6324109, Length=434, Percent_Identity=38.7096774193548, Blast_Score=305, Evalue=1e-83,
Organism=Drosophila melanogaster, GI21356033, Length=429, Percent_Identity=42.1911421911422, Blast_Score=322, Evalue=3e-88,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_NITMU (Q2YBX2)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411141.1
- ProteinModelPortal:   Q2YBX2
- SMR:   Q2YBX2
- STRING:   Q2YBX2
- GeneID:   3785909
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0441
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   YVLGIIK
- PhylomeDB:   Q2YBX2
- ProtClustDB:   PRK01117
- BioCyc:   NMUL323848:NMUL_A0441-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47354; Mature: 47223

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 14-14 ACT_SITE 42-42 BINDING 130-130 BINDING 144-144 BINDING 225-225 BINDING 240-240 BINDING 304-304 BINDING 306-306

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNVVVIGTQWGDEGKGKIVDWLTDQAQGVVRFQGGHNAGHTLVIGGKQTVLHLIPSGI
CCCCEEEEEECCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHH
LRKDVACYIGNGVVVSPQALLDEVGMLERAGIDVLSRLRISEACPLILPCHVALDNAREI
HHHHHHEEECCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHH
ARGLGKIGTTGRGIGPAYEDKVARRAVRLQDLFHRDRFAAKLGEILDYHNFVLKNYFQSP
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
VVDFQQTMDETLSLVERIRPMVADVPRLLFEANRAGANLLFEGAQGALLDIDHGTYPFVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCCCCCCEE
SSNCIAGAATTGSGIGPQMLHYVLGITKAYTTRVGAGPFPTELDDDVGRHLAKRGNEFGA
CCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCC
TTGRPRRCGWFDAAALKRSIQINGVSGLCVTKLDVMDGVETLRLGVGYKMMGKGEEEKFS
CCCCCCCCCCHHHHHHHHEEEECCCCCCHHHHHHHHHCHHHHHHCCCEEECCCCCHHHHH
AIMPVGAEELASCEPVYEEMPGWSGSTVGIRNFEQLPMAARNYLKRMEEVCEVSIDMIST
EECCCCHHHHHCCCHHHHHCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCC
GPDREETIVLRHPFE
CCCCCCEEEEECCCC
>Mature Secondary Structure 
SKNVVVIGTQWGDEGKGKIVDWLTDQAQGVVRFQGGHNAGHTLVIGGKQTVLHLIPSGI
CCCEEEEEECCCCCCCCCCHHHHHHCCCCEEEEECCCCCCCEEEECCHHHHHHHHHHHH
LRKDVACYIGNGVVVSPQALLDEVGMLERAGIDVLSRLRISEACPLILPCHVALDNAREI
HHHHHHEEECCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHCCHHHH
ARGLGKIGTTGRGIGPAYEDKVARRAVRLQDLFHRDRFAAKLGEILDYHNFVLKNYFQSP
HHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCH
VVDFQQTMDETLSLVERIRPMVADVPRLLFEANRAGANLLFEGAQGALLDIDHGTYPFVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCCCCCCEE
SSNCIAGAATTGSGIGPQMLHYVLGITKAYTTRVGAGPFPTELDDDVGRHLAKRGNEFGA
CCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCC
TTGRPRRCGWFDAAALKRSIQINGVSGLCVTKLDVMDGVETLRLGVGYKMMGKGEEEKFS
CCCCCCCCCCHHHHHHHHEEEECCCCCCHHHHHHHHHCHHHHHHCCCEEECCCCCHHHHH
AIMPVGAEELASCEPVYEEMPGWSGSTVGIRNFEQLPMAARNYLKRMEEVCEVSIDMIST
EECCCCHHHHHCCCHHHHHCCCCCCCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHCC
GPDREETIVLRHPFE
CCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA