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The map label for this gene is rsmE [H]

Identifier: 82701513

GI number: 82701513

Start: 415519

End: 416286

Strand: Direct

Name: rsmE [H]

Synonym: Nmul_A0379

Alternate gene names: 82701513

Gene position: 415519-416286 (Clockwise)

Preceding gene: 82701506

Following gene: 82701514

Centisome position: 13.05

GC content: 55.86

Gene sequence:

>768_bases
ATGACGCTTCCCCGCTTTTACTGCCCTATCGAAATTCTTGCGGGACAGACCCTGGAATTGCCCTCAGCAGTTGCGCATCA
TGCCGCTCGCGTCCTGAGACTCGAGGCAGGCGATGAAGTCGTGTTGTTCAATGGCGACGGTGGAGAATTCACGTGTGTCA
TTTTGCGAGCCAGCAAGCAGGGTGTGGATGTTGTCGTCGAAAGGCATGTACAGGTCGAGCGCGAATCCCCGCTTACCATT
CTGCTTGCTCAAGCGGTGTGCATGGGTGAAAAGATGGACTGGATCGTGCAGAAAGCAGTGGAACTGGGTGTAACCCGGAT
TCAGCCTCTCATTACCAAGCAGAGTGTCGTGAGACTTGGCGGCGAACGCGGGGAAAAACGCGTGAGCCATTTGCAAAAAG
TCGTTATCTCCGCATGCGAGCAATGCGGCAGAAACCATGTGCCGGAAGTGCTGCCGCTCGCGACCCTCCGGAACTGGCTG
GGTAGGCAAACCGAAGCACGCAGAATGTCCAGTGACAGTCTTTCCCCCGATGCTTGCTTCATGCTCTCGCCTTTTGCAAC
AAAGGGTTTGCGGGATTTCAAGCACTCTTCGAATATCGGCTCGATCATTTTGCTCGTGGGGCCCGAAGGAGGGTTTACCC
AGGATGAGGAAGCGGCTGCAATGGTTGCGGGTTTCATTCCATTGCGTCTGGGTCACCGCATACTGCGAACGGAGAGCGCC
GCACTGGCGGCAGTGGGAGCAATGCAGGCGCTATGGGGTGATTATTGA

Upstream 100 bases:

>100_bases
CGGCCATCTCGACAGCGTTTGTTCAAGCAGGACTGCTGAGACCCAATACTATCATGAGAAAGGCGCATTGATCGCTCCAC
CGTCCGATACTGAATATGCA

Downstream 100 bases:

>100_bases
TCATGGATTTTGTGACCTGGTTCCGGTCGGTAGCGCCTTATATCAACAGCTTCCGTCATAAAGTTTTCGTAGTCGCTTTC
GGCGGGGAAGTCGTTGCGGA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MTLPRFYCPIEILAGQTLELPSAVAHHAARVLRLEAGDEVVLFNGDGGEFTCVILRASKQGVDVVVERHVQVERESPLTI
LLAQAVCMGEKMDWIVQKAVELGVTRIQPLITKQSVVRLGGERGEKRVSHLQKVVISACEQCGRNHVPEVLPLATLRNWL
GRQTEARRMSSDSLSPDACFMLSPFATKGLRDFKHSSNIGSIILLVGPEGGFTQDEEAAAMVAGFIPLRLGHRILRTESA
ALAAVGAMQALWGDY

Sequences:

>Translated_255_residues
MTLPRFYCPIEILAGQTLELPSAVAHHAARVLRLEAGDEVVLFNGDGGEFTCVILRASKQGVDVVVERHVQVERESPLTI
LLAQAVCMGEKMDWIVQKAVELGVTRIQPLITKQSVVRLGGERGEKRVSHLQKVVISACEQCGRNHVPEVLPLATLRNWL
GRQTEARRMSSDSLSPDACFMLSPFATKGLRDFKHSSNIGSIILLVGPEGGFTQDEEAAAMVAGFIPLRLGHRILRTESA
ALAAVGAMQALWGDY
>Mature_254_residues
TLPRFYCPIEILAGQTLELPSAVAHHAARVLRLEAGDEVVLFNGDGGEFTCVILRASKQGVDVVVERHVQVERESPLTIL
LAQAVCMGEKMDWIVQKAVELGVTRIQPLITKQSVVRLGGERGEKRVSHLQKVVISACEQCGRNHVPEVLPLATLRNWLG
RQTEARRMSSDSLSPDACFMLSPFATKGLRDFKHSSNIGSIILLVGPEGGFTQDEEAAAMVAGFIPLRLGHRILRTESAA
LAAVGAMQALWGDY

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=254, Percent_Identity=38.1889763779528, Blast_Score=168, Evalue=3e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27804; Mature: 27672

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLPRFYCPIEILAGQTLELPSAVAHHAARVLRLEAGDEVVLFNGDGGEFTCVILRASKQ
CCCCCCCCCHHHHCCCCCCCHHHHHHHHHHHEEEECCCEEEEEECCCCEEEEEEEECCCC
GVDVVVERHVQVERESPLTILLAQAVCMGEKMDWIVQKAVELGVTRIQPLITKQSVVRLG
CHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GERGEKRVSHLQKVVISACEQCGRNHVPEVLPLATLRNWLGRQTEARRMSSDSLSPDACF
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEE
MLSPFATKGLRDFKHSSNIGSIILLVGPEGGFTQDEEAAAMVAGFIPLRLGHRILRTESA
EECHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ALAAVGAMQALWGDY
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLPRFYCPIEILAGQTLELPSAVAHHAARVLRLEAGDEVVLFNGDGGEFTCVILRASKQ
CCCCCCCCHHHHCCCCCCCHHHHHHHHHHHEEEECCCEEEEEECCCCEEEEEEEECCCC
GVDVVVERHVQVERESPLTILLAQAVCMGEKMDWIVQKAVELGVTRIQPLITKQSVVRLG
CHHHHHHHHHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GERGEKRVSHLQKVVISACEQCGRNHVPEVLPLATLRNWLGRQTEARRMSSDSLSPDACF
CCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCEE
MLSPFATKGLRDFKHSSNIGSIILLVGPEGGFTQDEEAAAMVAGFIPLRLGHRILRTESA
EECHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
ALAAVGAMQALWGDY
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8332061 [H]