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The map label for this gene is rlmH

Identifier: 82701496

GI number: 82701496

Start: 393886

End: 394356

Strand: Direct

Name: rlmH

Synonym: Nmul_A0362

Alternate gene names: 82701496

Gene position: 393886-394356 (Clockwise)

Preceding gene: 82701495

Following gene: 82701497

Centisome position: 12.37

GC content: 56.26

Gene sequence:

>471_bases
ATGAAATTCCTCGTTTATGCGGTGGGACATAAAATGCCGGAGTGGATTGCCGCGGGTTTTCAGGAATATGCCAAACGGAT
GCCACGTGAGGCGAATATCGAGCTTCTGGAAATCAAGCCTGAGAGACGGGATAGCGGAAAAAAAGTGGAACAGCTGCTGG
CCGCCGAGGGCGCACGTATCCGTGCGCTGCTGCCTTCCAATTGTCGGCTTGTGGTAATGGACGAGCGCGGGAGCCAATGG
ACTACCGCCGGTTTGGCCCATGCCATTGGCAGTTGGATGAAGGATGGAGGTGACACCGCGTTCCTCATTGGCGGTGCCGA
CGGGCTAGATCCGGCCCTCAGGAATGCGGCGGATGAAGTCCTGGCGTTATCTGCCCTGACACTTCCTCATGGGCTGGTTC
GAATTCTGCTGGCGGAACAACTCTACCGCGCGATATCGCTGATCAAGGGTCATCCTTATCATCGCGCATAA

Upstream 100 bases:

>100_bases
ATATGGGAGCGAGCGCCAAGTTCCCCGCGTGGGCGTCTCTCGAACAAACTCTTCCCGGAGCATTGGATAGGCGTGTTTTC
AGAATGAAATTCCTTGTCGC

Downstream 100 bases:

>100_bases
AAAATAACAATGTAACAGAAGTAACTGCGCTCCCGTAAGCATTCACTCCGCATGACTTTTGTGGAAAATCGCATCTATCT
TGCTTCCCGCAGTCCCCGCC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MKFLVYAVGHKMPEWIAAGFQEYAKRMPREANIELLEIKPERRDSGKKVEQLLAAEGARIRALLPSNCRLVVMDERGSQW
TTAGLAHAIGSWMKDGGDTAFLIGGADGLDPALRNAADEVLALSALTLPHGLVRILLAEQLYRAISLIKGHPYHRA

Sequences:

>Translated_156_residues
MKFLVYAVGHKMPEWIAAGFQEYAKRMPREANIELLEIKPERRDSGKKVEQLLAAEGARIRALLPSNCRLVVMDERGSQW
TTAGLAHAIGSWMKDGGDTAFLIGGADGLDPALRNAADEVLALSALTLPHGLVRILLAEQLYRAISLIKGHPYHRA
>Mature_156_residues
MKFLVYAVGHKMPEWIAAGFQEYAKRMPREANIELLEIKPERRDSGKKVEQLLAAEGARIRALLPSNCRLVVMDERGSQW
TTAGLAHAIGSWMKDGGDTAFLIGGADGLDPALRNAADEVLALSALTLPHGLVRILLAEQLYRAISLIKGHPYHRA

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=155, Percent_Identity=45.8064516129032, Blast_Score=141, Evalue=2e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_NITMU (Q2YC51)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411062.1
- ProteinModelPortal:   Q2YC51
- SMR:   Q2YC51
- STRING:   Q2YC51
- GeneID:   3784554
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0362
- eggNOG:   COG1576
- HOGENOM:   HBG622807
- OMA:   TANHPYH
- PhylomeDB:   Q2YC51
- BioCyc:   NMUL323848:NMUL_A0362-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR016051
- InterPro:   IPR003742
- PIRSF:   PIRSF004505
- TIGRFAMs:   TIGR00246

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 17154; Mature: 17154

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLVYAVGHKMPEWIAAGFQEYAKRMPREANIELLEIKPERRDSGKKVEQLLAAEGARI
CCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEE
RALLPSNCRLVVMDERGSQWTTAGLAHAIGSWMKDGGDTAFLIGGADGLDPALRNAADEV
EEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
LALSALTLPHGLVRILLAEQLYRAISLIKGHPYHRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKFLVYAVGHKMPEWIAAGFQEYAKRMPREANIELLEIKPERRDSGKKVEQLLAAEGARI
CCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCEE
RALLPSNCRLVVMDERGSQWTTAGLAHAIGSWMKDGGDTAFLIGGADGLDPALRNAADEV
EEECCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
LALSALTLPHGLVRILLAEQLYRAISLIKGHPYHRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA