Click here to switch to the map view.

The map label for this gene is aceF [H]

Identifier: 82701493

GI number: 82701493

Start: 391164

End: 392516

Strand: Direct

Name: aceF [H]

Synonym: Nmul_A0359

Alternate gene names: 82701493

Gene position: 391164-392516 (Clockwise)

Preceding gene: 82701492

Following gene: 82701494

Centisome position: 12.28

GC content: 54.99

Gene sequence:

>1353_bases
GTGGCGGAGACTAAACAGGTATTGATTCCCGATATTGGCGACTTTAAAGACGTTCCCATAATAGAAGTGCTGGTGAAAGC
CGGCGATTCGATCAAGGCGGAAGACTCCCTGATTGTGCTTGAATCGGACAAGGCCACCATAGAAGTGCCTTCTCCTTTTG
CTGGCATAATCAGAGAGTTATCCGTAAAGGTGGGCGATAAGGTATCGGAAGGTTCGCCCATCCTGACACTGGAAGCTTCA
GAAGCGGAGCAAGCGCCGCCCGCTGAGCCACGAGAAGCGGCACCCGCTTCAACGCCGGCTCCCGCTCCAACCACCGCTTC
TCCGGAACAGGCGCCGCGGCCGGCGACGCAACCTCGTGCGCAATCCCAATCTTCTGCGCAACCCCAATCATCGGGTTCTT
CTCCACGCTCTGCATTCGTTCCTTCTCCGATAGATGAAGCTACGTTTGCGAAGGCACACGCCAGTCCTTCGGTTCGACGC
TTTGCGCGCGAACTTGGCGTGAATCTGGGGCTGGTAAAGGGTAGCGGTGCCAAGCAGCGCATTCTCAAGGAAGATGTGCA
GTCTTTTGTCAAGACTGAACTCTCCAAGCCAAGGGGTAGCGGGACCGAGCTCAATCTGCTGCCCTGGCCTCAACCCGATT
TTGCGAAATTCGGTCCCGTGGAATTTAAGCCGCTATCGCGGATCAAAAAAATATCCGGAGCGAATCTGCACCGCAACTGG
GTCATGATTCCACACGTGACGCAGTTCGACGAAGCGGATATTACCGAGCTGGAAACCCTGCGCAAGGAAACAAACGAATC
TTCAAAAGAAGAAGGGGTGAAAGTTACTCTGCTCGCGTTTCTCCTGCGGGCTTCGATAGCGGCCCTAAAGAAGTTTCCCG
AGTTCAATGCCTCGCTGACCAGTGAAGGTGATGAAATGAATCTCGTGGTCAAGAATTATTACCATCTCGGCTTTGCAGCG
GATACACCTCATGGACTGGTGGTCCCCGTGATTCGGGATGTGGAAAAGAAAGGGGTCATCGCCATTGCCAAGGAAATGTC
TGATCTCGCAGCTTCGGCGCGGGCAGGCAAACTCAAGCCCACCGATATGCAGGGGGCGAGTTTTACCATTTCCAGCCTCG
GGGGCATCGGCGGCACTGCGTTCACGCCCATTATCAATGCGCCGGAAGTGGCGATTCTCGGTGTCTCGCGCGCAGTGATG
AAGCCTGTTTATCGGGATGGCGAATTTGTCCCGCGCCTGATGCTGCCATTATCCCTTTCCTATGATCATAGAGTAATCGA
CGGGGCGACAGCAGCGCGCTTTACGACGCACCTGGTCGAAGTGCTGGCTGATCTGCGTCGTGTGCTGTTGTAA

Upstream 100 bases:

>100_bases
CTGCACTACCAGGAGTCGGAGCGCTTGAAAGGCGGGATCCTCCTGCTGTGATCATTTCGTTGTGGAAAGCCGGGATTATC
TAATACGAGAAGGAAGAGGC

Downstream 100 bases:

>100_bases
AACACAGATTATTCATGGAAAGCGGGGAAGGCAAGTGAAGACAGCTGCCGGCTTTTTTGTCCGCTTGAGCAGCTTCGCAT
GACCAGCGTTGTTCCCGCCC

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 450; Mature: 449

Protein sequence:

>450_residues
MAETKQVLIPDIGDFKDVPIIEVLVKAGDSIKAEDSLIVLESDKATIEVPSPFAGIIRELSVKVGDKVSEGSPILTLEAS
EAEQAPPAEPREAAPASTPAPAPTTASPEQAPRPATQPRAQSQSSAQPQSSGSSPRSAFVPSPIDEATFAKAHASPSVRR
FARELGVNLGLVKGSGAKQRILKEDVQSFVKTELSKPRGSGTELNLLPWPQPDFAKFGPVEFKPLSRIKKISGANLHRNW
VMIPHVTQFDEADITELETLRKETNESSKEEGVKVTLLAFLLRASIAALKKFPEFNASLTSEGDEMNLVVKNYYHLGFAA
DTPHGLVVPVIRDVEKKGVIAIAKEMSDLAASARAGKLKPTDMQGASFTISSLGGIGGTAFTPIINAPEVAILGVSRAVM
KPVYRDGEFVPRLMLPLSLSYDHRVIDGATAARFTTHLVEVLADLRRVLL

Sequences:

>Translated_450_residues
MAETKQVLIPDIGDFKDVPIIEVLVKAGDSIKAEDSLIVLESDKATIEVPSPFAGIIRELSVKVGDKVSEGSPILTLEAS
EAEQAPPAEPREAAPASTPAPAPTTASPEQAPRPATQPRAQSQSSAQPQSSGSSPRSAFVPSPIDEATFAKAHASPSVRR
FARELGVNLGLVKGSGAKQRILKEDVQSFVKTELSKPRGSGTELNLLPWPQPDFAKFGPVEFKPLSRIKKISGANLHRNW
VMIPHVTQFDEADITELETLRKETNESSKEEGVKVTLLAFLLRASIAALKKFPEFNASLTSEGDEMNLVVKNYYHLGFAA
DTPHGLVVPVIRDVEKKGVIAIAKEMSDLAASARAGKLKPTDMQGASFTISSLGGIGGTAFTPIINAPEVAILGVSRAVM
KPVYRDGEFVPRLMLPLSLSYDHRVIDGATAARFTTHLVEVLADLRRVLL
>Mature_449_residues
AETKQVLIPDIGDFKDVPIIEVLVKAGDSIKAEDSLIVLESDKATIEVPSPFAGIIRELSVKVGDKVSEGSPILTLEASE
AEQAPPAEPREAAPASTPAPAPTTASPEQAPRPATQPRAQSQSSAQPQSSGSSPRSAFVPSPIDEATFAKAHASPSVRRF
ARELGVNLGLVKGSGAKQRILKEDVQSFVKTELSKPRGSGTELNLLPWPQPDFAKFGPVEFKPLSRIKKISGANLHRNWV
MIPHVTQFDEADITELETLRKETNESSKEEGVKVTLLAFLLRASIAALKKFPEFNASLTSEGDEMNLVVKNYYHLGFAAD
TPHGLVVPVIRDVEKKGVIAIAKEMSDLAASARAGKLKPTDMQGASFTISSLGGIGGTAFTPIINAPEVAILGVSRAVMK
PVYRDGEFVPRLMLPLSLSYDHRVIDGATAARFTTHLVEVLADLRRVLL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=451, Percent_Identity=29.9334811529933, Blast_Score=175, Evalue=6e-44,
Organism=Homo sapiens, GI31711992, Length=431, Percent_Identity=31.322505800464, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI19923748, Length=228, Percent_Identity=34.2105263157895, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI203098816, Length=218, Percent_Identity=30.2752293577982, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI203098753, Length=218, Percent_Identity=30.2752293577982, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI260898739, Length=188, Percent_Identity=30.3191489361702, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1786305, Length=454, Percent_Identity=47.5770925110132, Blast_Score=403, Evalue=1e-113,
Organism=Escherichia coli, GI1786946, Length=446, Percent_Identity=29.1479820627803, Blast_Score=171, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI17537937, Length=436, Percent_Identity=29.3577981651376, Blast_Score=177, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI17560088, Length=436, Percent_Identity=30.9633027522936, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI25146366, Length=207, Percent_Identity=37.1980676328502, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17538894, Length=310, Percent_Identity=30.6451612903226, Blast_Score=123, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6320352, Length=431, Percent_Identity=29.4663573085847, Blast_Score=160, Evalue=4e-40,
Organism=Saccharomyces cerevisiae, GI6324258, Length=383, Percent_Identity=26.3707571801567, Blast_Score=107, Evalue=5e-24,
Organism=Drosophila melanogaster, GI18859875, Length=437, Percent_Identity=30.4347826086957, Blast_Score=175, Evalue=7e-44,
Organism=Drosophila melanogaster, GI24645909, Length=211, Percent_Identity=35.0710900473934, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI20129315, Length=227, Percent_Identity=30.3964757709251, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24582497, Length=227, Percent_Identity=30.3964757709251, Blast_Score=118, Evalue=7e-27,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 48256; Mature: 48125

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETKQVLIPDIGDFKDVPIIEVLVKAGDSIKAEDSLIVLESDKATIEVPSPFAGIIREL
CCCCCCEECCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCEEECCCCHHHHHHHH
SVKVGDKVSEGSPILTLEASEAEQAPPAEPREAAPASTPAPAPTTASPEQAPRPATQPRA
HHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
QSQSSAQPQSSGSSPRSAFVPSPIDEATFAKAHASPSVRRFARELGVNLGLVKGSGAKQR
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCEEEEEECCCHHHH
ILKEDVQSFVKTELSKPRGSGTELNLLPWPQPDFAKFGPVEFKPLSRIKKISGANLHRNW
HHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCE
VMIPHVTQFDEADITELETLRKETNESSKEEGVKVTLLAFLLRASIAALKKFPEFNASLT
EEECCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
SEGDEMNLVVKNYYHLGFAADTPHGLVVPVIRDVEKKGVIAIAKEMSDLAASARAGKLKP
CCCCCEEEEEEEEEEEEEECCCCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCC
TDMQGASFTISSLGGIGGTAFTPIINAPEVAILGVSRAVMKPVYRDGEFVPRLMLPLSLS
CCCCCCEEEHHHCCCCCCCHHCCCCCCCCEEEEECHHHHHHHHHCCCCHHHHHHCCEECC
YDHRVIDGATAARFTTHLVEVLADLRRVLL
CCCEEECCCHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
AETKQVLIPDIGDFKDVPIIEVLVKAGDSIKAEDSLIVLESDKATIEVPSPFAGIIREL
CCCCCEECCCCCCCCCCHHHHHHHHCCCCCCCCCCEEEEECCCCEEECCCCHHHHHHHH
SVKVGDKVSEGSPILTLEASEAEQAPPAEPREAAPASTPAPAPTTASPEQAPRPATQPRA
HHHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
QSQSSAQPQSSGSSPRSAFVPSPIDEATFAKAHASPSVRRFARELGVNLGLVKGSGAKQR
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHCCEEEEEECCCHHHH
ILKEDVQSFVKTELSKPRGSGTELNLLPWPQPDFAKFGPVEFKPLSRIKKISGANLHRNW
HHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCE
VMIPHVTQFDEADITELETLRKETNESSKEEGVKVTLLAFLLRASIAALKKFPEFNASLT
EEECCCCCCCCCCHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCC
SEGDEMNLVVKNYYHLGFAADTPHGLVVPVIRDVEKKGVIAIAKEMSDLAASARAGKLKP
CCCCCEEEEEEEEEEEEEECCCCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCC
TDMQGASFTISSLGGIGGTAFTPIINAPEVAILGVSRAVMKPVYRDGEFVPRLMLPLSLS
CCCCCCEEEHHHCCCCCCCHHCCCCCCCCEEEEECHHHHHHHHHCCCCHHHHHHCCEECC
YDHRVIDGATAARFTTHLVEVLADLRRVLL
CCCEEECCCHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]