Click here to switch to the map view.

The map label for this gene is folD

Identifier: 82701491

GI number: 82701491

Start: 387357

End: 388223

Strand: Direct

Name: folD

Synonym: Nmul_A0357

Alternate gene names: 82701491

Gene position: 387357-388223 (Clockwise)

Preceding gene: 82701490

Following gene: 82701492

Centisome position: 12.16

GC content: 56.06

Gene sequence:

>867_bases
ATGAGCGCGAAGATCATTGACGGCAAGAGGTTGAGCCAGGAGGTGAGGGCGGAGTGCCGGGCACGCGCAGCGCGTTTGGC
GGAAAAAGGTACCCAGCCAGGACTGGCGGTGGTTATCGTGGGAGACAATCCGGCTTCCAGGGTGTATGTGCGAAACAAGG
CTAAGGCCTGTGGCGAAACGGGCATCTATTCCGAAGTCCACGAATTCCCGGAAAATGCCAACCAGGATGAGGTGATTGGA
CGCATTCAGGAATTGAACGGGAATCCCGGAATTCACGGCATCCTCGTTCAATTGCCTCTGCCGCGGCATTTCGACAGCAG
CAGAGTCACCGAGTCTGTGGCCGTGGAAAAAGATGTGGATGGCTTCCATCTTTATAATGTAGGCGCACTTGTAACCGGTA
GCAGCATTTTTCCGCCTTGCACGCCTAACGGCGTGATGAAAATGCTGGAAAAATATGATATTCCGATCGAGGGGCAGCAT
GCGGTAATAGTAGGCCGCAGTAATATCGTCGGTAAACCGATGGCGCTGATGCTGCTGCAAAAAGGCGCAACCGTTACCAT
CTGCACGTCCAGAACGCGGAATCTGGCGGAACACACCAGCCGCGCCGATATTCTGATAGTTGCTGCGGGCAAGCCGCGCC
TGATCACCGCCATGATGGTGAAACCGGGAGCGACAGTAATAGACGTCGGCATCAACCGGCTGTCTGACGGGAAGCTGGCG
GGAGATGTGGACTTTGAATCGGTCAAGGAAAAGGCGGGTTACATCACGCCTGTGCCGGGGGGAGTAGGTCCGATGACCAT
TGCGATGCTGCTGTGCAACACCGTTGTGGCAGCCGAGCAGGCGCACGCCCGATTACAGGGTAATTGA

Upstream 100 bases:

>100_bases
TGCAGGGTTTCGCAGGATATACAGCCAGAGTGAAAGATACGAACAGCCGGAGCATTTTACGTCATTCGCCCGTTCTCTGC
CCTGCCGCGCCCGGACCATA

Downstream 100 bases:

>100_bases
CAGCGCCTCTATCACATCGGGCAAGCGCCCGACAATTTCGAGACAAGACATGGAACCTATACCGGATATAGACCCGACTG
AAACCCAGGAATGGCTGGAG

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase

Number of amino acids: Translated: 288; Mature: 287

Protein sequence:

>288_residues
MSAKIIDGKRLSQEVRAECRARAARLAEKGTQPGLAVVIVGDNPASRVYVRNKAKACGETGIYSEVHEFPENANQDEVIG
RIQELNGNPGIHGILVQLPLPRHFDSSRVTESVAVEKDVDGFHLYNVGALVTGSSIFPPCTPNGVMKMLEKYDIPIEGQH
AVIVGRSNIVGKPMALMLLQKGATVTICTSRTRNLAEHTSRADILIVAAGKPRLITAMMVKPGATVIDVGINRLSDGKLA
GDVDFESVKEKAGYITPVPGGVGPMTIAMLLCNTVVAAEQAHARLQGN

Sequences:

>Translated_288_residues
MSAKIIDGKRLSQEVRAECRARAARLAEKGTQPGLAVVIVGDNPASRVYVRNKAKACGETGIYSEVHEFPENANQDEVIG
RIQELNGNPGIHGILVQLPLPRHFDSSRVTESVAVEKDVDGFHLYNVGALVTGSSIFPPCTPNGVMKMLEKYDIPIEGQH
AVIVGRSNIVGKPMALMLLQKGATVTICTSRTRNLAEHTSRADILIVAAGKPRLITAMMVKPGATVIDVGINRLSDGKLA
GDVDFESVKEKAGYITPVPGGVGPMTIAMLLCNTVVAAEQAHARLQGN
>Mature_287_residues
SAKIIDGKRLSQEVRAECRARAARLAEKGTQPGLAVVIVGDNPASRVYVRNKAKACGETGIYSEVHEFPENANQDEVIGR
IQELNGNPGIHGILVQLPLPRHFDSSRVTESVAVEKDVDGFHLYNVGALVTGSSIFPPCTPNGVMKMLEKYDIPIEGQHA
VIVGRSNIVGKPMALMLLQKGATVTICTSRTRNLAEHTSRADILIVAAGKPRLITAMMVKPGATVIDVGINRLSDGKLAG
DVDFESVKEKAGYITPVPGGVGPMTIAMLLCNTVVAAEQAHARLQGN

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family

Homologues:

Organism=Homo sapiens, GI94721354, Length=294, Percent_Identity=46.5986394557823, Blast_Score=243, Evalue=1e-64,
Organism=Homo sapiens, GI222418558, Length=294, Percent_Identity=46.2585034013605, Blast_Score=241, Evalue=4e-64,
Organism=Homo sapiens, GI222136639, Length=296, Percent_Identity=41.8918918918919, Blast_Score=231, Evalue=4e-61,
Organism=Homo sapiens, GI36796743, Length=206, Percent_Identity=26.6990291262136, Blast_Score=82, Evalue=6e-16,
Organism=Escherichia coli, GI1786741, Length=283, Percent_Identity=51.2367491166078, Blast_Score=282, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI17568735, Length=301, Percent_Identity=40.8637873754153, Blast_Score=189, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6319558, Length=286, Percent_Identity=46.5034965034965, Blast_Score=265, Evalue=9e-72,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=41.6107382550336, Blast_Score=228, Evalue=7e-61,
Organism=Saccharomyces cerevisiae, GI6322933, Length=291, Percent_Identity=26.4604810996564, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17136816, Length=294, Percent_Identity=48.9795918367347, Blast_Score=254, Evalue=6e-68,
Organism=Drosophila melanogaster, GI17136818, Length=294, Percent_Identity=48.9795918367347, Blast_Score=254, Evalue=6e-68,
Organism=Drosophila melanogaster, GI24645718, Length=296, Percent_Identity=44.9324324324324, Blast_Score=229, Evalue=1e-60,
Organism=Drosophila melanogaster, GI17137370, Length=296, Percent_Identity=44.9324324324324, Blast_Score=229, Evalue=1e-60,
Organism=Drosophila melanogaster, GI62472483, Length=296, Percent_Identity=44.9324324324324, Blast_Score=229, Evalue=1e-60,
Organism=Drosophila melanogaster, GI45551871, Length=296, Percent_Identity=44.9324324324324, Blast_Score=229, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): FOLD_NITMU (Q2YC56)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411057.1
- ProteinModelPortal:   Q2YC56
- SMR:   Q2YC56
- STRING:   Q2YC56
- GeneID:   3784549
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0357
- eggNOG:   COG0190
- HOGENOM:   HBG328751
- OMA:   GIDNTKV
- PhylomeDB:   Q2YC56
- ProtClustDB:   PRK14189
- BioCyc:   NMUL323848:NMUL_A0357-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01576
- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631
- Gene3D:   G3DSA:3.40.50.720
- PRINTS:   PR00085

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C

EC number: =1.5.1.5; =3.5.4.9

Molecular weight: Translated: 30692; Mature: 30561

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00766 THF_DHG_CYH_1; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAKIIDGKRLSQEVRAECRARAARLAEKGTQPGLAVVIVGDNPASRVYVRNKAKACGET
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEECCCHHHCCCC
GIYSEVHEFPENANQDEVIGRIQELNGNPGIHGILVQLPLPRHFDSSRVTESVAVEKDVD
CCHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCC
GFHLYNVGALVTGSSIFPPCTPNGVMKMLEKYDIPIEGQHAVIVGRSNIVGKPMALMLLQ
CEEEEECCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHH
KGATVTICTSRTRNLAEHTSRADILIVAAGKPRLITAMMVKPGATVIDVGINRLSDGKLA
CCCEEEEECCCCHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCEEEEECCCCCCCCCEE
GDVDFESVKEKAGYITPVPGGVGPMTIAMLLCNTVVAAEQAHARLQGN
CCCCHHHHHHHCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SAKIIDGKRLSQEVRAECRARAARLAEKGTQPGLAVVIVGDNPASRVYVRNKAKACGET
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEECCCHHHCCCC
GIYSEVHEFPENANQDEVIGRIQELNGNPGIHGILVQLPLPRHFDSSRVTESVAVEKDVD
CCHHHHHHCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHCCCCC
GFHLYNVGALVTGSSIFPPCTPNGVMKMLEKYDIPIEGQHAVIVGRSNIVGKPMALMLLQ
CEEEEECCEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCHHHHHHHH
KGATVTICTSRTRNLAEHTSRADILIVAAGKPRLITAMMVKPGATVIDVGINRLSDGKLA
CCCEEEEECCCCHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCEEEEECCCCCCCCCEE
GDVDFESVKEKAGYITPVPGGVGPMTIAMLLCNTVVAAEQAHARLQGN
CCCCHHHHHHHCCEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA