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The map label for this gene is ilvD

Identifier: 82701480

GI number: 82701480

Start: 378025

End: 379698

Strand: Reverse

Name: ilvD

Synonym: Nmul_A0346

Alternate gene names: 82701480

Gene position: 379698-378025 (Counterclockwise)

Preceding gene: 82701488

Following gene: 82701479

Centisome position: 11.92

GC content: 58.96

Gene sequence:

>1674_bases
ATGTCAGATAACCAGCGCAGCCGGGCAATCACCCAGGGGGCGGAACGTACCCCCAACCGCGCGATGCTGCGGGCCGTAGG
TTTCAGCGACAACGATTTCGACAAACCAATCGTCGGCGTGGCCAATGGCTTTTCCACCATCACACCCTGCAACAAGGGAT
TGAACGAACTGGCGCTGGCTGCCGAGCAGGCATTGAAACAGGCGGCTGCGATGCCCCAGATGTTCGGTACCATCACCGTT
TCCGATGGCATCTCCATGGGAACGGAGGGCATGAAGTATTCACTGGTATCCCGCGAAGTCATTGCCGACTCGATCGAAAC
GGCGGTGCAGGCCGAAAGCATGGATGGCGTGATCGCCATCGGGGGCTGTGACAAGAACATGCCAGGAGCAATGATCGCCA
TTGCGCGCATGAATGTTCCCGCAATTTTTGTTTATGGCGGAACGATTAAACCGGGCCACTATAAAGGAAAGGATCTCACT
ATTGTCAGCGCATTCGAAGCCGTCGGACAATATACCTCCCACAAGATAGACGCGAAAGAACTGCTCGAAGTGGAGCGCCA
CGCCTGTCCGGGAGCCGGCTCCTGCGGAGGCATGTTCACTGCCAATACGATGTCGTCCGCTTTCGAAGCGATGGGCATGA
GTCTCCCCTATTCTTCCACGATGGCCGCGGAGGATGGCGAGAAGCTAACCAGCGCGGCGCGCTCCGCGGAAGTGCTCGTG
GATGCCATCAGGAAACAGATTCGCCCGCGCGACATCATTACCCGCAAGTCCATTGAAAACGCGATCGCGGTGATCATGGC
CGTGGGTGGTTCCACCAATGCTGTTTTGCACTTCCTCGCCATTGCCCATGCGGCCGAAGTGACACTCACCATCGATGATT
TTGAACGCATGCGCGGCAAAGTGCCGGTACTGTGCGATCTCAAACCTTCGGGACGGTACGTTGCCACCGACCTGCACAAG
GCAGGGGGCATTCCCCAGGTCATGAAAATGCTGCTCGACCACGGCCTGTTGCATGGCGACTGCATCACCATCAGCGGACA
GACCATTGCCGAGATATTGAAGGATGTGCCGTCAGAGCCTCGCGAAGATCAGGATGTCATTCGCCAGTGGGACAACCCGT
TGTACGTCCAGGGCCACCTCGCCATACTCAAGGGCAATCTCGCCCCAGAAGGGTGCGTGGCGAAAATCACCGGGGTGAAA
TCCCCAAAAATTACCGGACCGGCACGCGTATTCGATTCGGAAGAAGCCTGCATGGCAGCTATCCTTGCGCGGGAGATCCA
GCCCGGCGACGTGGTGGTGATACGCTATGAGGGACCCAAGGGCGGCCCCGGCATGCGGGAAATGCTGTCTCCCACTTCCG
CGCTTATTGGCGAGGGACTCGGAGATTCGGTGGGCCTGATCACCGATGGACGATTCTCCGGGGGCACCTATGGAATGGTG
GTTGGACACGTGGCGCCGGAGGCGTTCGTAGGCGGGACCATTGCGCTGGTGCGAGAAGGCGATTCGATTACCATAGACGC
CGAGCAACGGCTGCTGCAGCTCAACATTCCCGGAGATGAGCTGGCTCGGCGCCGGGCTGAATGGCAACCGCCCCATCCGC
GCTACACCCGGGGAGTGCTCGCTAAATATTCGAAGCTCGTCTCGAGTGCCAGCCGCGGCGCAATCACCGACTAG

Upstream 100 bases:

>100_bases
TTTCCTCACCGCTTCGCAGCCGCCACGGGCGCTTCTTCCCCTGACCGGAATTCGCCAGAATTTGCCTGCATTCCCGTTTT
TTCAATCCAAGGAGTAACTC

Downstream 100 bases:

>100_bases
TCCGTTTTTTCCACCACAAAGCCATGCCCAACCATCTTGCCGGTGAAACCAGCCCCTACCTGCTGCAGCATGCAGATAAT
CCTGTCGACTGGTATCCCTG

Product: dihydroxy-acid dehydratase

Products: NA

Alternate protein names: DAD

Number of amino acids: Translated: 557; Mature: 556

Protein sequence:

>557_residues
MSDNQRSRAITQGAERTPNRAMLRAVGFSDNDFDKPIVGVANGFSTITPCNKGLNELALAAEQALKQAAAMPQMFGTITV
SDGISMGTEGMKYSLVSREVIADSIETAVQAESMDGVIAIGGCDKNMPGAMIAIARMNVPAIFVYGGTIKPGHYKGKDLT
IVSAFEAVGQYTSHKIDAKELLEVERHACPGAGSCGGMFTANTMSSAFEAMGMSLPYSSTMAAEDGEKLTSAARSAEVLV
DAIRKQIRPRDIITRKSIENAIAVIMAVGGSTNAVLHFLAIAHAAEVTLTIDDFERMRGKVPVLCDLKPSGRYVATDLHK
AGGIPQVMKMLLDHGLLHGDCITISGQTIAEILKDVPSEPREDQDVIRQWDNPLYVQGHLAILKGNLAPEGCVAKITGVK
SPKITGPARVFDSEEACMAAILAREIQPGDVVVIRYEGPKGGPGMREMLSPTSALIGEGLGDSVGLITDGRFSGGTYGMV
VGHVAPEAFVGGTIALVREGDSITIDAEQRLLQLNIPGDELARRRAEWQPPHPRYTRGVLAKYSKLVSSASRGAITD

Sequences:

>Translated_557_residues
MSDNQRSRAITQGAERTPNRAMLRAVGFSDNDFDKPIVGVANGFSTITPCNKGLNELALAAEQALKQAAAMPQMFGTITV
SDGISMGTEGMKYSLVSREVIADSIETAVQAESMDGVIAIGGCDKNMPGAMIAIARMNVPAIFVYGGTIKPGHYKGKDLT
IVSAFEAVGQYTSHKIDAKELLEVERHACPGAGSCGGMFTANTMSSAFEAMGMSLPYSSTMAAEDGEKLTSAARSAEVLV
DAIRKQIRPRDIITRKSIENAIAVIMAVGGSTNAVLHFLAIAHAAEVTLTIDDFERMRGKVPVLCDLKPSGRYVATDLHK
AGGIPQVMKMLLDHGLLHGDCITISGQTIAEILKDVPSEPREDQDVIRQWDNPLYVQGHLAILKGNLAPEGCVAKITGVK
SPKITGPARVFDSEEACMAAILAREIQPGDVVVIRYEGPKGGPGMREMLSPTSALIGEGLGDSVGLITDGRFSGGTYGMV
VGHVAPEAFVGGTIALVREGDSITIDAEQRLLQLNIPGDELARRRAEWQPPHPRYTRGVLAKYSKLVSSASRGAITD
>Mature_556_residues
SDNQRSRAITQGAERTPNRAMLRAVGFSDNDFDKPIVGVANGFSTITPCNKGLNELALAAEQALKQAAAMPQMFGTITVS
DGISMGTEGMKYSLVSREVIADSIETAVQAESMDGVIAIGGCDKNMPGAMIAIARMNVPAIFVYGGTIKPGHYKGKDLTI
VSAFEAVGQYTSHKIDAKELLEVERHACPGAGSCGGMFTANTMSSAFEAMGMSLPYSSTMAAEDGEKLTSAARSAEVLVD
AIRKQIRPRDIITRKSIENAIAVIMAVGGSTNAVLHFLAIAHAAEVTLTIDDFERMRGKVPVLCDLKPSGRYVATDLHKA
GGIPQVMKMLLDHGLLHGDCITISGQTIAEILKDVPSEPREDQDVIRQWDNPLYVQGHLAILKGNLAPEGCVAKITGVKS
PKITGPARVFDSEEACMAAILAREIQPGDVVVIRYEGPKGGPGMREMLSPTSALIGEGLGDSVGLITDGRFSGGTYGMVV
GHVAPEAFVGGTIALVREGDSITIDAEQRLLQLNIPGDELARRRAEWQPPHPRYTRGVLAKYSKLVSSASRGAITD

Specific function: Valine and isoleucine biosynthesis; fourth step. [C]

COG id: COG0129

COG function: function code EG; Dihydroxyacid dehydratase/phosphogluconate dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ilvD/edd family

Homologues:

Organism=Escherichia coli, GI48994964, Length=606, Percent_Identity=38.6138613861386, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1786464, Length=469, Percent_Identity=36.4605543710021, Blast_Score=215, Evalue=5e-57,
Organism=Escherichia coli, GI1788157, Length=482, Percent_Identity=33.402489626556, Blast_Score=214, Evalue=2e-56,
Organism=Escherichia coli, GI2367371, Length=512, Percent_Identity=33.59375, Blast_Score=200, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6322476, Length=562, Percent_Identity=49.4661921708185, Blast_Score=542, Evalue=1e-155,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ILVD_NITMU (Q2YC67)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_411046.1
- ProteinModelPortal:   Q2YC67
- SMR:   Q2YC67
- STRING:   Q2YC67
- GeneID:   3785971
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A0346
- eggNOG:   COG0129
- HOGENOM:   HBG671001
- OMA:   NMPGAMI
- PhylomeDB:   Q2YC67
- ProtClustDB:   PRK00911
- BioCyc:   NMUL323848:NMUL_A0346-MONOMER
- HAMAP:   MF_00012
- InterPro:   IPR015928
- InterPro:   IPR004404
- InterPro:   IPR000581
- InterPro:   IPR020558
- PANTHER:   PTHR21000
- TIGRFAMs:   TIGR00110

Pfam domain/function: PF00920 ILVD_EDD; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.9

Molecular weight: Translated: 59121; Mature: 58989

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00886 ILVD_EDD_1; PS00887 ILVD_EDD_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNQRSRAITQGAERTPNRAMLRAVGFSDNDFDKPIVGVANGFSTITPCNKGLNELALA
CCCCHHHHHHHHCCCCCCCHHHHEEECCCCCCCCCCEEECCCCCCCCCCHHCCHHHHHHH
AEQALKQAAAMPQMFGTITVSDGISMGTEGMKYSLVSREVIADSIETAVQAESMDGVIAI
HHHHHHHHHHCHHHHEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEE
GGCDKNMPGAMIAIARMNVPAIFVYGGTIKPGHYKGKDLTIVSAFEAVGQYTSHKIDAKE
CCCCCCCCCCEEEEEECCCCEEEEECCEECCCCCCCCCEEEEHHHHHHHHHHCCCCCHHH
LLEVERHACPGAGSCGGMFTANTMSSAFEAMGMSLPYSSTMAAEDGEKLTSAARSAEVLV
HHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
DAIRKQIRPRDIITRKSIENAIAVIMAVGGSTNAVLHFLAIAHAAEVTLTIDDFERMRGK
HHHHHHCCCHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHCEEEEEHHHHHHHCCC
VPVLCDLKPSGRYVATDLHKAGGIPQVMKMLLDHGLLHGDCITISGQTIAEILKDVPSEP
CCEEEECCCCCCEEEEHHHHHCCCHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCCC
REDQDVIRQWDNPLYVQGHLAILKGNLAPEGCVAKITGVKSPKITGPARVFDSEEACMAA
CCHHHHHHHCCCCEEEEEEEEEEECCCCCCCCEEEEECCCCCCCCCCHHHCCCHHHHHHH
ILAREIQPGDVVVIRYEGPKGGPGMREMLSPTSALIGEGLGDSVGLITDGRFSGGTYGMV
HHHHCCCCCCEEEEEECCCCCCCCHHHHHCHHHHHHHCCCCCCCCEEECCCCCCCEEEEE
VGHVAPEAFVGGTIALVREGDSITIDAEQRLLQLNIPGDELARRRAEWQPPHPRYTRGVL
EECCCCHHHCCCEEEEEECCCEEEEECCCEEEEEECCHHHHHHHHCCCCCCCCHHHHHHH
AKYSKLVSSASRGAITD
HHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SDNQRSRAITQGAERTPNRAMLRAVGFSDNDFDKPIVGVANGFSTITPCNKGLNELALA
CCCHHHHHHHHCCCCCCCHHHHEEECCCCCCCCCCEEECCCCCCCCCCHHCCHHHHHHH
AEQALKQAAAMPQMFGTITVSDGISMGTEGMKYSLVSREVIADSIETAVQAESMDGVIAI
HHHHHHHHHHCHHHHEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCEEEE
GGCDKNMPGAMIAIARMNVPAIFVYGGTIKPGHYKGKDLTIVSAFEAVGQYTSHKIDAKE
CCCCCCCCCCEEEEEECCCCEEEEECCEECCCCCCCCCEEEEHHHHHHHHHHCCCCCHHH
LLEVERHACPGAGSCGGMFTANTMSSAFEAMGMSLPYSSTMAAEDGEKLTSAARSAEVLV
HHHHHHHCCCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
DAIRKQIRPRDIITRKSIENAIAVIMAVGGSTNAVLHFLAIAHAAEVTLTIDDFERMRGK
HHHHHHCCCHHHHHHHHHHHHEEEEEEECCCHHHHHHHHHHHHHCEEEEEHHHHHHHCCC
VPVLCDLKPSGRYVATDLHKAGGIPQVMKMLLDHGLLHGDCITISGQTIAEILKDVPSEP
CCEEEECCCCCCEEEEHHHHHCCCHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHCCCCC
REDQDVIRQWDNPLYVQGHLAILKGNLAPEGCVAKITGVKSPKITGPARVFDSEEACMAA
CCHHHHHHHCCCCEEEEEEEEEEECCCCCCCCEEEEECCCCCCCCCCHHHCCCHHHHHHH
ILAREIQPGDVVVIRYEGPKGGPGMREMLSPTSALIGEGLGDSVGLITDGRFSGGTYGMV
HHHHCCCCCCEEEEEECCCCCCCCHHHHHCHHHHHHHCCCCCCCCEEECCCCCCCEEEEE
VGHVAPEAFVGGTIALVREGDSITIDAEQRLLQLNIPGDELARRRAEWQPPHPRYTRGVL
EECCCCHHHCCCEEEEEECCCEEEEECCCEEEEEECCHHHHHHHHCCCCCCCCHHHHHHH
AKYSKLVSSASRGAITD
HHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA